{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T02:19:16Z","timestamp":1783477156941,"version":"3.55.0"},"reference-count":67,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2025,11,22]],"date-time":"2025-11-22T00:00:00Z","timestamp":1763769600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"European Union\u2014NextGenerationEU\u2014National Recovery and Resilience Plan","award":["ECS00000017"],"award-info":[{"award-number":["ECS00000017"]}]},{"name":"European Union\u2014NextGenerationEU\u2014National Recovery and Resilience Plan","award":["CUP B83C22003920001"],"award-info":[{"award-number":["CUP B83C22003920001"]}]},{"name":"Ministry of University and Research"},{"name":"National Recovery and Resilience Plan (NRRP), and project MNESYS"},{"name":"project MNESYS","award":["PE0000006"],"award-info":[{"award-number":["PE0000006"]}]},{"name":"A Multiscale integrated approach to the study of the nervous system in health and disease","award":["DR. 1553 11.10.2022"],"award-info":[{"award-number":["DR. 1553 11.10.2022"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Brain tumors (BTs), including glioblastoma (GBM) and meningioma (MGM), contribute significantly to the global cancer burden. The microbiome has been implicated in carcinogenesis, yet its role in BTs remains underexplored. We performed 16S rRNA gene sequencing of the gut microbiota (GM) and intratumoral microbiome (ItM) from fresh tissue samples of 9 patients with GBM and 18 with MGM. 12 age- and sex-matched healthy controls (HCs) were also enrolled. GM profiling revealed reduced alpha diversity and distinct microbial communities in BT patients versus HCs. Notably, Verrucomicrobiota and Synergistaceae were enriched, while Lachnospiraceae, Peptostreptococcaceae, and Muribacter spp. were depleted. GBM patients showed reductions in Peptostreptococcaceae and the Eubacterium hallii group, while MGM patients had increased Synergistia and Erysipelatoclostridium. Compared with MGM, GBM patients were enriched in Peptostreptococcales\u2013Tissierellales, Coprobacillus, and Peptoniphilus but depleted in Weissella. Venn analysis revealed 176 genera shared across groups with unique taxa distinguishing tumor patients and HCs. ItM profiling revealed enrichment of Proteobacteria, Actinomycetota, and Campylobacterota in GBM, while MGM contained higher levels of Bacillota and Bacteroidota. GBM tissues harbored Burkholderia-Caballeronia-Paraburkholderia, Helicobacter, and Leifsonia, whereas MGM tissues were dominated by Bacteroides and Blautia. Notably, stool and tumor samples shared 91 genera in GBM and 105 in MGM. This study provides novel insights by (i) characterizing ItM from fresh samples, (ii) comparing ItM profiles of GBM and MGM, (iii) linking GM and ItM within the same patients, and (iv) suggesting potential clinical implications for BT management.<\/jats:p>","DOI":"10.3390\/ijms262311290","type":"journal-article","created":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T09:02:07Z","timestamp":1763974927000},"page":"11290","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Specific Intratumoral Microbiome Signatures in Human Glioblastoma and Meningioma: Evidence for a Gut\u2013Brain Microbial Axis"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0751-6072","authenticated-orcid":false,"given":"Dalila","family":"Mehelleb","sequence":"first","affiliation":[{"name":"University of Bejaia, Faculty of Natural and Life Sciences, Bejaia 06000, Algeria"},{"name":"University of Bejaia, Laboratory of Microbial Ecology, Bejaia 06000, Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4819-9568","authenticated-orcid":false,"given":"Abderezak","family":"Ghidouche","sequence":"additional","affiliation":[{"name":"University of Bejaia, Faculty of Natural and Life Sciences, Bejaia 06000, Algeria"},{"name":"University of Bejaia, Laboratory of Cancer Bioengineering, Bejaia 06000, Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5151-2618","authenticated-orcid":false,"given":"Simone","family":"Baldi","sequence":"additional","affiliation":[{"name":"Department of Experimental and Clinical Medicine, University of Florence, 50134 Florence, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ferhat","family":"Djoudi","sequence":"additional","affiliation":[{"name":"University of Bejaia, Faculty of Natural and Life Sciences, Bejaia 06000, Algeria"},{"name":"University of Bejaia, Laboratory of Microbial Ecology, Bejaia 06000, Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-2267-7551","authenticated-orcid":false,"given":"Sara","family":"Bertorello","sequence":"additional","affiliation":[{"name":"Department of Experimental and Clinical Medicine, University of Florence, 50134 Florence, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8630-8166","authenticated-orcid":false,"given":"Leandro","family":"Di Gloria","sequence":"additional","affiliation":[{"name":"Department of Biomedical, Experimental and Clinical Sciences \u201cMario Serio\u201d, University of Florence, 50134 Florence, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6862-9409","authenticated-orcid":false,"given":"Matteo","family":"Ramazzotti","sequence":"additional","affiliation":[{"name":"Department 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