{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T13:21:49Z","timestamp":1785331309290,"version":"3.55.0"},"reference-count":48,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2025,11,25]],"date-time":"2025-11-25T00:00:00Z","timestamp":1764028800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007197","name":"U.S. Public Health Service","doi-asserted-by":"crossref","award":["R01AI169865"],"award-info":[{"award-number":["R01AI169865"]}],"id":[{"id":"10.13039\/100007197","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Presented here are additional explanations for five key points offered by Rojero et al. in their 2025 publication in this journal, regarding characteristics of hyper-aggressive phage 0524phi7-1. These are (i) that the \u201cbypassing of the evolution of host resistance\u201d has been seen in other phages, especially dual-receptor generalist phages; (ii) that the \u201cclearing of semi-turbid plaques\u201d could be due to a phenomenon known as lysis inhibition collapse, (iii) that the \u201cformation of satellite plaques\u201d is reminiscent of the morphology of plaques generated by phage T4 star mutants, (iv) that \u201cmulti-day plaque enlargement\u201d has been seen in other phages such as phage T7 but may also be explained by other phenomena including endolysin release, (v) that suggestions of phage \u201cswimming\u201d could be explained by virion diffusion within empty volumes found within maturing bacterial lawns. In particular, phage plaques that display lysis inhibition can influence the surrounding bacterial lawn well beyond their visible region. This presumably occurs via a reaction\u2013diffusion mechanism whose leading edge of virion diffusion fails to display lysis inhibition, but which leaves in its wake lysis-inhibited bacterial infections that may not lyse in a timely manner. Phage-infected bacteria thus may be found well beyond a plaque\u2019s visible boundaries, along with diffusing endolysin.<\/jats:p>","DOI":"10.3390\/ijms262311368","type":"journal-article","created":{"date-parts":[[2025,11,25]],"date-time":"2025-11-25T08:50:20Z","timestamp":1764060620000},"page":"11368","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Dual-Receptor Recognition, Lysis Inhibition, Endolysin Release, and Reaction\u2013Diffusion as Alternative Explanations. Comment on Rojero et al. Bypassing Evolution of Bacterial Resistance to Phages: The Example of Hyper-Aggressive Phage 0524phi7-1. Int. J. Mol. Sci. 2025, 26, 2914"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9151-2375","authenticated-orcid":false,"given":"Stephen T.","family":"Abedon","sequence":"first","affiliation":[{"name":"Department of Microbiology, The Ohio State University, Mansfield, OH 44906, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,11,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Rojero, M., Weaver-Rosen, M., and Serwer, P. (2025). Bypassing evolution of bacterial resistance to phages: The example of hyper-aggressive phage 0524phi7-1. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms26072914"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1111\/eva.13518","article-title":"Comparison of bacterial suppression by phage cocktails, dual-receptor generalists, and coevolutionarily trained phages","volume":"16","author":"Borin","year":"2023","journal-title":"Evol. Appl."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/genetics\/107.1.1","article-title":"Two-step resistance by Escherichia coli B to bacteriophage T2","volume":"107","author":"Lenski","year":"1984","journal-title":"Genetics"},{"key":"ref_4","first-page":"724","article-title":"Host range mutants of bacteriophage Ox2 can use two different outer membrane proteins of Escherichia coli K-12 as receptors","volume":"159","author":"Morona","year":"1984","journal-title":"J. Bacteriol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"10384","DOI":"10.1128\/JVI.00801-12","article-title":"A multivalent adsorption apparatus explains the broad host range of phage phi92: A comprehensive genomic and structural analysis","volume":"86","author":"Schwarzer","year":"2012","journal-title":"J. Virol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1046\/j.1365-2958.2001.02491.x","article-title":"An ancient player unmasked: T4 rI encodes a t-specific antiholin","volume":"41","author":"Ramanculov","year":"2001","journal-title":"Mol. Microbiol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"7618","DOI":"10.1128\/JB.00854-07","article-title":"The T4 RI antiholin has an N-terminal signal anchor release domain that targets it for degradation by DegP","volume":"189","author":"Tran","year":"2007","journal-title":"J. Bacteriol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"3537","DOI":"10.1128\/JB.00138-11","article-title":"The bacteriophage T4 rapid-lysis genes and their mutational proclivities","volume":"193","author":"Burch","year":"2011","journal-title":"J. Bacteriol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2448","DOI":"10.1128\/JB.00294-16","article-title":"The last r locus unveiled: T4 RIII is a cytoplasmic antiholin","volume":"198","author":"Chen","year":"2016","journal-title":"J. Bacteriol."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Abedon, S.T. (2019). Look who\u2019s talking: T-even phage lysis inhibition, the granddaddy of virus-virus intercellular communication research. Viruses, 11.","DOI":"10.3390\/v11100951"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"8073","DOI":"10.1128\/jb.174.24.8073-8080.1992","article-title":"Lysis of lysis inhibited bacteriophage T4-infected cells","volume":"174","author":"Abedon","year":"1992","journal-title":"J. Bacteriol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1016\/S0922-338X(97)81129-4","article-title":"Synchronized disruption of Escherichia coli cells by T4 phage infection","volume":"83","author":"Asami","year":"1997","journal-title":"J. Ferment. Bioeng."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1017\/S0016672399003833","article-title":"Bacteriophage T4 resistance to lysis-inhibition collapse","volume":"74","author":"Abedon","year":"1999","journal-title":"Genet. Res."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1007\/s11262-010-0532-1","article-title":"A role for accessory genes rI.-1 and rI.1 in the regulation of lysis inhibition by bacteriophage T4","volume":"41","author":"Golec","year":"2010","journal-title":"Virus Genes"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1128\/jvi.8.1.87-94.1971","article-title":"Enlargement of Escherichia coli after bacteriophage infection I. description of phenomenon","volume":"8","author":"Freedman","year":"1971","journal-title":"J. Virol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1128\/jvi.8.1.95-102.1971","article-title":"Enlargement of Escherichia coli after bacteriophage infection II. proposed mechanism","volume":"8","author":"Freedman","year":"1971","journal-title":"J. Virol."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Abedon, S.T. (2008). Impact of spatial structure on phage population growth. Bacteriophage Ecology, Cambridge University Press.","DOI":"10.1017\/CBO9780511541483"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1099\/00221287-18-2-330","article-title":"The properties of a star mutant of phage T2","volume":"18","author":"Symonds","year":"1958","journal-title":"J. Gen. Microbiol."},{"key":"ref_19","first-page":"112","article-title":"Star mutants of the bacteriophage T4B","volume":"7","author":"Krylov","year":"1971","journal-title":"Genetika"},{"key":"ref_20","first-page":"85","article-title":"Genetic and physiological study of amber mutants in gene stII of T4B phage","volume":"8","author":"Krylov","year":"1972","journal-title":"Genetika"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1128\/jvi.15.1.22-26.1975","article-title":"Mutations in the new gene stIlI of bacteriophage T4B suppressing the lysis defect of gene stIl and gene e mutants","volume":"15","author":"Krylov","year":"1975","journal-title":"J. Virol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1111\/j.1469-185X.1946.tb00451.x","article-title":"Bacterial viruses or bacteriophages","volume":"21","year":"1946","journal-title":"Biol. Rev."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1009","DOI":"10.1016\/0006-291X(91)91519-I","article-title":"A quantifiable phenotype of viral propagation","volume":"174","author":"Yin","year":"1991","journal-title":"Biochem. Biophys. Res. Com."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1128\/jb.55.2.257-276.1948","article-title":"Lysis and lysis inhibition with Escherichia coli bacteriophage","volume":"55","author":"Doermann","year":"1948","journal-title":"J. Bacteriol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"e53200","DOI":"10.7554\/eLife.53200","article-title":"Dominant Vibrio cholerae phage exhibits lysis inhibition sensitive to disruption by a defensive phage satellite","volume":"9","author":"Hays","year":"2020","journal-title":"eLife"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Sultan-Alolama, M.I., Amin, A., Vijayan, R., and El-Tarabily, K.A. (2023). Isolation, characterization, and comparative genomic analysis of bacteriophage Ec_MI-02 from pigeon feces infecting Escherichia coli O157:H7. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24119506"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Danis-Wlodarczyk, K.M., Wozniak, D.J., and Abedon, S.T. (2021). Treating bacterial infections with bacteriophage-based enzybiotics: In vitro, in vivo and clinical application. Antibiotics, 10.","DOI":"10.3390\/antibiotics10121497"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"518","DOI":"10.1099\/00221287-18-2-518","article-title":"The mechanism of lysis by phage studied with defective lysogenic bacteria","volume":"18","author":"Jacob","year":"1958","journal-title":"J. Gen. Microbiol."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Guti\u00e9rrez, D., Ruas-Madiedo, P., Mart\u00ednez, B., Rodr\u00edguez, A., and Garc\u00eda, P. (2014). Effective removal of staphylococcal biofilms by the endolysin LysH5. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0107307"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Alreja, A.B., Appel, A.E., Zhu, J.C., Riley, S.P., Gonzalez-Juarbe, N., and Nelson, D.C. (2024). SP-CHAP, an endolysin with enhanced activity against biofilm pneumococci and nasopharyngeal colonization. mBio, 15.","DOI":"10.1128\/mbio.00069-24"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Liu, H., Wei, X., Wang, Z., Huang, X., Li, M., Hu, Z., Zhang, K., Hu, Q., Peng, H., and Shang, W. (2024). LysSYL: A broad-spectrum phage endolysin targeting Staphylococcus species and eradicating S. aureus biofilms. Microb. Cell Fact., 23.","DOI":"10.1186\/s12934-024-02359-4"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"46","DOI":"10.4161\/bact.1.1.13980","article-title":"Lysis from without","volume":"1","author":"Abedon","year":"2011","journal-title":"Bacteriophage"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Xu, H., Bao, X., Hong, W., Wang, A., Wang, K., Dong, H., Hou, J., Govinden, R., Deng, B., and Chenia, H.Y. (2021). Biological characterization and evolution of bacteriophage T7-\u0394holin during the serial passage process. Front. Microbiol., 12.","DOI":"10.3389\/fmicb.2021.705310"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Son, B., Kong, M., Lee, Y., and Ryu, S. (2020). Development of a novel chimeric endolysin, Lys109 with enhanced lytic activity against Staphylococcus aureus. Front. Microbiol., 11.","DOI":"10.3389\/fmicb.2020.615887"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1147","DOI":"10.2217\/fmb.12.97","article-title":"Bacteriophage endolysins as novel antimicrobials","volume":"7","author":"Schmelcher","year":"2012","journal-title":"Future Microbiol."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Nakonieczna, A., Topolska-Wos, A., and Lobocka, M. (2024). New bacteriophage-derived lysins, LysJ and LysF, with the potential to control Bacillus anthracis. Appl. Microbiol. Biotechnol., 108.","DOI":"10.1007\/s00253-023-12839-z"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Rodr\u00edguez-Rubio, L., Chang, W.L., Guti\u00e9rrez, D., Lavigne, R., Mart\u00ednez, B., Rodr\u00edguez, A., Govers, S.K., Aertsen, A., Hirl, C., and Biebl, M. (2016). \u2018Artilysation\u2019 of endolysin \u03bbSa2lys strongly improves its enzymatic and antibacterial activity against streptococci. Sci. Rep., 6.","DOI":"10.1038\/srep35382"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1016\/0022-5193(64)90056-6","article-title":"The growth of viral plaques during the enlargement phase","volume":"6","author":"Koch","year":"1964","journal-title":"J. Theor. Biol."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Abedon, S.T. (2016). Bacteriophage exploitation of bacterial biofilms: Phage preference for less mature targets?. FEMS Microbiol. Lett., 363.","DOI":"10.1093\/femsle\/fnv246"},{"key":"ref_40","unstructured":"Abedon, S.T. (2011). Bacteriophages and Biofilms: Ecology, Phage Therapy, Plaques, Nova Science Publishers."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1540","DOI":"10.1016\/S0006-3495(92)81958-6","article-title":"Replication of viruses in a growing plaque: A reaction-diffusion model","volume":"61","author":"Yin","year":"1992","journal-title":"Biophys. J."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1006\/jtbi.1999.1001","article-title":"Amplification and spread of viruses in a growing plaque","volume":"200","author":"You","year":"1999","journal-title":"J. Theor. Biol."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Bryan, D., El-Shibiny, A., Hobbs, Z., Porter, J., and Kutter, E.M. (2016). Bacteriophage T4 infection of stationary phase E. coli: Life after log from a phage perspective. Front. Microbiol., 7.","DOI":"10.3389\/fmicb.2016.01391"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1093\/genetics\/34.1.44","article-title":"Genetic recombination between host range and plaque-type mutants of bacteriophage in a single bacterial culture","volume":"34","author":"Hershey","year":"1949","journal-title":"Genetics"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Rajnovic, D., Munoz-Berbel, X., and Mas, J. (2019). Fast phage detection and quantification: An optical density-based approach. PLoS ONE, 14.","DOI":"10.1371\/journal.pone.0216292"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1128\/jvi.13.1.186-196.1974","article-title":"Development of coliphage N4: Ultrastructural studies","volume":"13","author":"Schito","year":"1974","journal-title":"J. Virol."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Kim, J., Kim, J., and Ryu, S. (2024). Elucidation of molecular function of phage protein responsible for optimization of host cell lysis. BMC Microbiol., 24.","DOI":"10.1186\/s12866-024-03684-9"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"620","DOI":"10.1093\/genetics\/31.6.620","article-title":"Mutation of bacteriophage with respect to type of plaque","volume":"31","author":"Hershey","year":"1946","journal-title":"Genetics"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/23\/11368\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,27]],"date-time":"2025-11-27T05:17:40Z","timestamp":1764220660000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/23\/11368"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,25]]},"references-count":48,"journal-issue":{"issue":"23","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["ijms262311368"],"URL":"https:\/\/doi.org\/10.3390\/ijms262311368","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,25]]}}}