{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,25]],"date-time":"2025-11-25T15:43:12Z","timestamp":1764085392663,"version":"3.45.0"},"reference-count":60,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2025,11,25]],"date-time":"2025-11-25T00:00:00Z","timestamp":1764028800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Chronic myeloid leukemia (CML) is a myeloproliferative neoplasm characterized by uncontrolled proliferation of myeloid cells. MicroRNAs (miRNAs), small noncoding RNAs, regulate post-transcriptional gene expression by degrading target mRNAs or repressing translation. Dysregulated miRNA expression has been implicated in various malignancies, including CML, where they can function as oncogenes or tumor suppressors. This study aimed to investigate the relationship between miR-122-5p and cell division cycle 25A (CDC25A) in CML and to elucidate the regulatory mechanisms of miR-122-5p. This study integrates bioinformatics analysis with in vitro RT-qPCR validation in K562 chronic myeloid leukemia cells to explore the potential regulatory relationship between miR-122-5p and CDC25A. mRNA expression profiles were retrieved from the GSE100026 dataset in the Gene Expression Omnibus (GEO), and differentially expressed genes were identified using GEO2R. Quantitative real-time PCR (RT-qPCR) was performed to measure miR-122-5p, CDC25A, and cyclin-dependent kinase 4 (CDK4) expression levels. Bioinformatics analyses (miRNeT, miRDIP, TargetScan, BioGPS, GeneMANIA, STRING) were applied to predict molecular interactions and functional pathways. Public RNA-seq datasets and in silico tools were used to prioritize candidates; RT-qPCR in a single CML cell line (K562) provided in vitro expression validation. In K562 cells, miR-122-5p expression was significantly reduced, while CDC25A and CDK4 were markedly upregulated. Bioinformatics tools confirmed CDC25A as a potential miR-122-5p target. Functional enrichment indicated CDC25A involvement in cell cycle regulation and apoptosis. These findings suggest that miR-122-5p functions as a tumor suppressor in CML by targeting CDC25A. Modulating the miR-122-5p\/CDC25A axis may provide potential molecular targets for inhibiting CML progression through regulation of cell cycle pathways. Findings are exploratory and based on bioinformatics with limited in vitro expression confirmation; functional studies are required to establish causality.<\/jats:p>","DOI":"10.3390\/ijms262311401","type":"journal-article","created":{"date-parts":[[2025,11,25]],"date-time":"2025-11-25T15:29:47Z","timestamp":1764084587000},"page":"11401","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Identification of a Novel miR-122-5p\/CDC25A Axis and Potential Therapeutic Targets for Chronic Myeloid Leukemia"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5089-0836","authenticated-orcid":false,"given":"Serap","family":"Ozer Yaman","sequence":"first","affiliation":[{"name":"Department of Medical Biochemistry, Trabzon Kanuni Training and Research Hospital, Faculty of Medicine, Trabzon University, 61030 Trabzon, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2503-1228","authenticated-orcid":false,"given":"Nina","family":"Petrovi\u0107","sequence":"additional","affiliation":[{"name":"Laboratory for Radiobiology and Molecular Genetics, \u201cVIN\u010cA\u201d Institute of Nuclear Sciences-National Institute of the Republic of Serbia, University of Belgrade, 11000 Belgrade, Serbia"},{"name":"Department of Experimental Oncology, Institute for Oncology and Radiology of Serbia, 11000 Belgrade, Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Selcuk","family":"Yaman","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry, Trabzon Kanuni Training and Research Hospital, Faculty of Medicine, Trabzon University, 61030 Trabzon, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Osman","family":"Akidan","sequence":"additional","affiliation":[{"name":"Department of Hematology, Meng\u00fccek Gazi Education and Research Hospital, 24100 Erzincan, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahmet","family":"Cimbek","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Trabzon Kanuni Training and Research Hospital, Faculty of Medicine, Trabzon University, 61030 Trabzon, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gulsah","family":"Baycelebi","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Trabzon Kanuni Training and Research Hospital, Faculty of Medicine, Trabzon University, 61030 Trabzon, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatjana","family":"Srdi\u0107-Raji\u0107","sequence":"additional","affiliation":[{"name":"Department of Experimental Oncology, Institute for Oncology and Radiology of Serbia, 11000 Belgrade, Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8178-3742","authenticated-orcid":false,"given":"Ahmad","family":"\u0160ami","sequence":"additional","affiliation":[{"name":"Cellular and Molecular Radiation Oncology Laboratory, Department of Radiation Oncology, Universitaetsmedizin Mannheim, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany"},{"name":"DKFZ-Hector Cancer Institute, University Medicine Mannheim, 68167 Mannheim, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5919-3295","authenticated-orcid":false,"given":"Sema","family":"Misir","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Pharmacy, Sivas Cumhuriyet University, 58140 Sivas, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,11,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2244856","DOI":"10.1080\/16078454.2023.2244856","article-title":"Bioinformatic analysis of the expression profile and identification of RhoGDI2 as a biomarker in imatinib-resistant K562 cells","volume":"28","author":"Yang","year":"2023","journal-title":"Hematology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1007\/s12185-022-03510-w","article-title":"Genetic landscape of chronic myeloid leukemia","volume":"117","author":"Ochi","year":"2023","journal-title":"Int. J. Hematol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"155161","DOI":"10.1016\/j.prp.2024.155161","article-title":"The impact of the BCR-ABL oncogene in the pathology and treatment of chronic myeloid leukemia","volume":"254","author":"Nsairat","year":"2024","journal-title":"Pathol. Res. Pract."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2191","DOI":"10.1002\/ajh.27443","article-title":"Chronic myeloid leukemia: 2025 update on diagnosis, therapy, and monitoring","volume":"99","author":"Jabbour","year":"2024","journal-title":"Am. J. Hematol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1007\/s00018-023-05036-8","article-title":"miR-181a plays the tumor-suppressor role in chronic myeloid leukemia CD34+ cells partially via SERPINE1","volume":"81","author":"Zhang","year":"2023","journal-title":"Cell Mol. Life Sci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1080\/17474086.2024.2440776","article-title":"Understanding and overcoming resistance to tyrosine kinase inhibitors (TKIs) in Chronic myeloid leukemia (CML)","volume":"18","author":"Scalzulli","year":"2025","journal-title":"Expert. Rev. Hematol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1297","DOI":"10.1080\/10428194.2016.1243676","article-title":"miRNAs in chronic myeloid leukemia: Small molecules, essential function","volume":"58","year":"2017","journal-title":"Leuk. Lymphoma"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Yang, H., Liu, Y., Chen, L., Zhao, J., Guo, M., Zhao, X., Wen, Z., He, Z., Chen, C., and Xu, L. (2023). MiRNA-Based Therapies for Lung Cancer: Opportunities and Challenges?. Biomolecules, 13.","DOI":"10.3390\/biom13060877"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Suri, C., Swarnkar, S., Bhaskar, L.V.K.S., and Verma, H.K. (2024). Non-Coding RNA as a Biomarker in Lung Cancer. Noncoding RNA, 10.","DOI":"10.3390\/ncrna10050050"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"120437","DOI":"10.1016\/j.lfs.2022.120437","article-title":"The role of microRNAs in the development, progression and drug resistance of chronic myeloid leukemia and their potential clinical significance","volume":"296","author":"Navabi","year":"2022","journal-title":"Life Sci."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Nie, Z.Y., Liu, X.J., Zhan, Y., Liu, M.H., Zhang, X.Y., Li, Z.Y., Lu, Y.-Q., Luo, J.-M., and Yang, L. (2019). MiR-140-5p induces cell apoptosis and decreases Warburg effect in chronic myeloid leukemia by targeting SIX1. Biosci. Rep., 39.","DOI":"10.1042\/BSR20190150"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1186\/s12935-023-02868-z","article-title":"MicroRNA-122 in human cancers: From mechanistic to clinical perspectives","volume":"23","author":"Faramin","year":"2023","journal-title":"Cancer Cell Int."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1038\/s41418-021-00845-5","article-title":"A novel CDC25A\/DYRK2 regulatory switch modulates cell cycle and survival","volume":"29","author":"Ponce","year":"2022","journal-title":"Cell Death Differ."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"392","DOI":"10.1016\/j.bbrc.2019.03.082","article-title":"MiR-365 enhances the radiosensitivity of non-small cell lung cancer cells through targeting CDC25A","volume":"512","author":"Li","year":"2019","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"3526","DOI":"10.1002\/jcp.26906","article-title":"MicroRNA-99a-5p suppresses breast cancer progression and cell-cycle pathway through downregulating CDC25A","volume":"234","author":"Qin","year":"2019","journal-title":"J. Cell Physiol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"7128","DOI":"10.1158\/0008-5472.CAN-05-2552","article-title":"Cell adhesion regulates CDC25A expression and proliferation in acute myeloid leukemia","volume":"66","author":"Ysebaert","year":"2006","journal-title":"Cancer Res."},{"key":"ref_17","first-page":"743","article-title":"Silencing CDC25A inhibits the proliferation of liver cancer cells by downregulating IL-6 in vitro and in vivo","volume":"45","author":"Chen","year":"2020","journal-title":"Int. J. Mol. Med."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"631","DOI":"10.2174\/187152012800617678","article-title":"The role of Cdc25A in the regulation of cell proliferation and apoptosis","volume":"12","author":"Shen","year":"2012","journal-title":"Anticancer Agents Med. Chem."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Dakilah, I., Harb, A., Abu-Gharbieh, E., El-Huneidi, W., Taneera, J., Hamoudi, R., Semreen, M.H., and Bustanji, Y. (2024). Potential of CDC25 phosphatases in cancer research and treatment: Key to precision medicine. Front. Pharmacol., 15.","DOI":"10.3389\/fphar.2024.1324001"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Zhang, J., Liu, Y., Li, Q., Xu, A., Hu, Y., and Sun, C. (2022). Ferroptosis in hematological malignancies and its potential network with abnormal tumor metabolism. Biomed. Pharmacother., 148.","DOI":"10.1016\/j.biopha.2022.112747"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1038\/s41408-023-00853-3","article-title":"Global burden of hematologic malignancies and evolution patterns over the past 30 years","volume":"13","author":"Zhang","year":"2023","journal-title":"Blood Cancer J."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1016\/j.jare.2020.04.016","article-title":"Transcriptome profiling reveals the high incidence of hnRNPA1 exon 8 inclusion in chronic myeloid leukemia","volume":"24","author":"Li","year":"2020","journal-title":"J. Adv. Res."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Chakrabortty, A., Patton, D.J., Smith, B.F., and Agarwal, P. (2023). miRNAs: Potential as biomarkers and therapeutic targets for cancer. Genes, 14.","DOI":"10.20944\/preprints202306.0045.v1"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1038\/cgt.2014.44","article-title":"The malignancy suppression role of miR-23a by targeting the BCR\/ABL oncogene in chromic myeloid leukemia","volume":"21","author":"Xishan","year":"2014","journal-title":"Cancer Gene Ther."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1094","DOI":"10.1016\/j.yexcr.2013.02.002","article-title":"MiR-29b suppresses CML cell proliferation and induces apoptosis via regulation of BCR\/ABL1 protein","volume":"319","author":"Li","year":"2013","journal-title":"Exp. Cell Res."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1007\/s12672-024-01408-z","article-title":"Circ_0001047 inhibits prostate cancer progression and enhances abiraterone sensitivity via miR-122-5p\/FKBP5\/PHLPP1\/AKT axis in vitro","volume":"15","author":"Chen","year":"2024","journal-title":"Discov. Oncol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1007\/s10142-024-01403-1","article-title":"TGF-\u03b21-Induced LINC01094 promotes epithelial-mesenchymal transition in hepatocellular carcinoma through the miR-122-5p\/TGFBR2\u2013SAMD2\u2013SMAD3 Axis","volume":"24","author":"Yang","year":"2024","journal-title":"Funct. Integr. Genomics."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Salzmann, R.J., Garbin, A., Gaffo, E., Elia, C., Martire, G., Bortoluzzi, S., Tondo, A., Muggeo, P., Sala, A., and Pizzi, M. (2024). Extracellular Vesicle miR-122-5p as a Prognostic Biomarker in Pediatric Classical Hodgkin Lymphoma. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms252413243"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1166","DOI":"10.1016\/j.prp.2017.06.017","article-title":"Decreased expression of microRNA-122 is associated with an unfavorable prognosis in childhood acute myeloid leukemia and function analysis indicates a therapeutic potential","volume":"213","author":"Yang","year":"2017","journal-title":"Pathol. Res. Pract."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"2919","DOI":"10.1002\/jcb.26838","article-title":"CDC25A pathway toward tumorigenesis: Molecular targets of CDC25A in cell-cycle regulation","volume":"120","author":"Sadeghi","year":"2019","journal-title":"J. Cell Biochem."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1869","DOI":"10.1002\/2211-5463.12730","article-title":"miR-122-5p modulates the radiosensitivity of cervical cancer cells by regulating cell division cycle 25A (CDC25A)","volume":"9","author":"Ding","year":"2019","journal-title":"FEBS Open Bio."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/S1535-6108(03)00048-5","article-title":"Chk1 regulates the S phase checkpoint by coupling the physiological turnover and ionizing radiation-induced accelerated proteolysis of Cdc25A","volume":"3","author":"Falck","year":"2003","journal-title":"Cancer Cell"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Li, J., Qiao, H., Wu, F., Sun, S., Feng, C., Li, C., Yan, W., Lv, W., Wu, H., and Liu, M. (2022). A novel hypoxia- and lactate metabolism-related signature to predict prognosis and immunotherapy responses for breast cancer by integrating machine learning and bioinformatic analyses. Front. Immunol., 13.","DOI":"10.3389\/fimmu.2022.998140"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Tian, Y., Xing, Y., Zhang, Z., Peng, R., Zhang, L., and Sun, Y. (2020). Bioinformatics Analysis of Key Genes and circRNA-miRNA-mRNA Regulatory Network in Gastric Cancer. Biomed. Res. Int., 2020.","DOI":"10.1155\/2020\/2862701"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1007\/s11912-022-01199-y","article-title":"Targeting apoptosis in cancer","volume":"24","author":"Singh","year":"2022","journal-title":"Curr. Oncol. Rep."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1038\/s41580-021-00404-3","article-title":"Cell cycle control in cancer","volume":"23","author":"Matthews","year":"2022","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"109","DOI":"10.3892\/mmr.2023.12996","article-title":"CDC25A inhibition suppresses cell proliferation and induces G 1\/S-phase cell cycle arrest in nasopharyngeal carcinoma","volume":"27","author":"Wang","year":"2023","journal-title":"Mol. Med. Rep."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1043","DOI":"10.1016\/j.chembiol.2004.07.007","article-title":"Cdc25 Phosphatases and Cancer","volume":"11","author":"Rudolph","year":"2004","journal-title":"Chem. Biol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"993","DOI":"10.1016\/j.molcel.2011.08.021","article-title":"MicroRNA Destabilization Enables Dynamic Regulation of the miR-16 Family in Response to Cell Cycle Changes","volume":"43","author":"Rissland","year":"2011","journal-title":"Mol. Cell"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"7713","DOI":"10.1158\/0008-5472.CAN-07-1083","article-title":"The let-7 microRNA represses cell proliferation pathways in human cells","volume":"67","author":"Johnson","year":"2007","journal-title":"Cancer Res."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"3714","DOI":"10.1172\/JCI34922","article-title":"MicroRNA15a modulates expression of the cell-cycle regulator Cdc25A and affects hepatic cystogenesis in a rat model of polycystic kidney disease","volume":"118","author":"Lee","year":"2008","journal-title":"J. Clin. Investig."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"2388","DOI":"10.1101\/gad.1819009","article-title":"miR-449a and miR-449b are direct transcriptional targets of E2F1 and negatively regulate pRb\u2013E2F1 activity through a feedback loop by targeting CDK6 and CDC25A","volume":"23","author":"Yang","year":"2009","journal-title":"Genes Dev."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"8157","DOI":"10.1158\/0008-5472.CAN-09-1996","article-title":"miR21 negatively regulates CDC25A and cell cylce progression in colpon cancer cells","volume":"69","author":"Wan","year":"2009","journal-title":"Cancer Res."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1016\/j.brainres.2009.11.056","article-title":"MiR-125b is critical for the suppression of human U251 glioma stem cell proliferation","volume":"1312","author":"Shi","year":"2010","journal-title":"Brain Res."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"2138","DOI":"10.1091\/mbc.e10-01-0062","article-title":"MiR-322\/424 and -503 are induced during muscle differentiation and promote cell cycle quiescence and differentiation by down-regulation of Cdc25A","volume":"21","author":"Sarkar","year":"2010","journal-title":"Mol. Biol. Cell"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"693","DOI":"10.1038\/ncb2241","article-title":"Control of vertebrate multiciliogenesis by miR-449 through direct repression of the Delta\/Notch pathway","volume":"13","author":"Marcet","year":"2011","journal-title":"Nat. Cell Biol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"800","DOI":"10.1038\/cdd.2013.5","article-title":"CDC25A targeting by miR-483-3p decreases CCND-CDK4\/6 assembly and contributes to cell cycle arrest","volume":"20","author":"Bertero","year":"2013","journal-title":"Cell Death Differ."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1186\/s12935-020-01304-w","article-title":"The role of CDC25C in cell cycle regulation and clinical cancer therapy: A systematic review","volume":"20","author":"Liu","year":"2020","journal-title":"Cancer Cell Int."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1093\/nar\/30.1.207","article-title":"Gene Expression Omnibus: NCBI gene expression and hybridization array data repository","volume":"30","author":"Edgar","year":"2002","journal-title":"Nucleic Acids Res."},{"key":"ref_50","first-page":"776","article-title":"Differential expression and functional analysis of lung cancer gene expression datasets: A systems biology perspective","volume":"18","author":"Bao","year":"2019","journal-title":"Oncol. Lett."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"W244","DOI":"10.1093\/nar\/gkaa467","article-title":"miRNet 2.0: Network-based visual analytics for miRNA functional analysis and systems biology","volume":"48","author":"Chang","year":"2020","journal-title":"Nucleic Acids Res."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"D360","DOI":"10.1093\/nar\/gkx1144","article-title":"MirDIP 4.1\u2014Integrative database of human microRNA target predictions","volume":"46","author":"Tokar","year":"2018","journal-title":"Nucleic Acids Res."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Shirdel, E.A., Xie, W., Mak, T.W., and Jurisica, I. (2011). NAViGaTing the micronome using multiple microRNA prediction databases to identify signalling pathway-associated microRNAs. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0017429"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"eaav1741","DOI":"10.1126\/science.aav1741","article-title":"The biochemical basis of microRNA targeting efficacy","volume":"366","author":"McGeary","year":"2019","journal-title":"Science"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"D313","DOI":"10.1093\/nar\/gkv1104","article-title":"BioGPS: Building your own mash-up of gene annotations and expression profiles","volume":"44","author":"Wu","year":"2016","journal-title":"Nucleic Acids Res."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Wu, C., Orozco, C., Boyer, J., Leglise, M., Goodale, J., Batalov, S., Hodge, C.L., Haase, J., Janes, J., and Huss, J.W. (2009). BioGPS: An extensible and customizable portal for querying and organizing gene annotation resources. Genome. Biol., 10.","DOI":"10.1186\/gb-2009-10-11-r130"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"867","DOI":"10.1182\/blood.2019000611","article-title":"Genomic landscape of neutrophilic leukemias of ambiguous diagnosis","volume":"134","author":"Zhang","year":"2019","journal-title":"Blood"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"D605","DOI":"10.1093\/nar\/gkaa1074","article-title":"The STRING database in 2021: Customizable protein\u2014Protein networks, and functional characterization of user-uploaded gene\/measurement sets","volume":"49","author":"Szklarczyk","year":"2021","journal-title":"Nucleic Acids Res."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1093\/nar\/gkq537","article-title":"The GeneMANIA prediction server: Biological network integration for gene prioritization and predicting gene function","volume":"38","author":"Donaldson","year":"2010","journal-title":"Nucleic Acids Res."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2\u2212\u0394\u0394CT method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/23\/11401\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,25]],"date-time":"2025-11-25T15:34:10Z","timestamp":1764084850000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/23\/11401"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,25]]},"references-count":60,"journal-issue":{"issue":"23","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["ijms262311401"],"URL":"https:\/\/doi.org\/10.3390\/ijms262311401","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,25]]}}}