{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T03:30:19Z","timestamp":1790307019799,"version":"4.1.0"},"reference-count":38,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2025,11,25]],"date-time":"2025-11-25T00:00:00Z","timestamp":1764028800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32261143734"],"award-info":[{"award-number":["32261143734"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010038","name":"Earmarked Fund for China Agriculture Research System","doi-asserted-by":"publisher","award":["CARS-38"],"award-info":[{"award-number":["CARS-38"]}],"id":[{"id":"10.13039\/501100010038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Endometrial stromal cell (ESC) plasticity is critical to embryo survival and conceptus elongation; however, their genetic regulation remains unclear. To elucidate the molecular mechanisms by which ESR1 regulates sheep fecundity through endometrial function, we investigated the effects of ESR1 on ovine ESCs in vitro. Tissue distribution analysis revealed elevated ESR1 expression in the reproductive organs, particularly the endometrium, compared with that in non-reproductive tissues. Both ESR1 mRNA and its encoded ER\u03b1 protein were significantly upregulated in the endometrium of high-fecundity Hu sheep compared to those in the endometrium of low-fecundity Hu sheep. Given the specific localisation of ER\u03b1 in ESCs, we examined the functional roles of ESR1 in these cells in vitro. ESR1 knockdown in ESCs inhibited cell proliferation and induced apoptosis, concomitant with increased Caspase3 and Caspase9 mRNA expression. Furthermore, ESR1 interference triggered G0\/G1-phase cell cycle arrest via the upregulation of P53 and P21 and significantly impaired cell migration capacity. Collectively, these results demonstrate that ESR1 critically regulates ESC proliferation, apoptosis, cell cycle progression, and migration. Our findings establish ESR1 as a key determinant of sheep fecundity and provide mechanistic insights into the high reproductive efficiency of Hu sheep.<\/jats:p>","DOI":"10.3390\/ijms262311410","type":"journal-article","created":{"date-parts":[[2025,11,25]],"date-time":"2025-11-25T16:31:54Z","timestamp":1764088314000},"page":"11410","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["ESR1 Regulates Fecundity and Functions in Sheep Endometrial Stromal Cells"],"prefix":"10.3390","volume":"26","author":[{"given":"Kang","family":"Li","sequence":"first","affiliation":[{"name":"Sanya Research Institute of Nanjing Agricultural University, Nanjing Agricultural University, Sanya 572000, China"},{"name":"Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Hohhot 010030, China"},{"name":"Jiangsu Livestock Embryo Engineering Laboratory, Nanjing Agricultural University, Nanjing 210095, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoxiao","family":"Gao","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8409-5534","authenticated-orcid":false,"given":"Zhibo","family":"Wang","sequence":"additional","affiliation":[{"name":"Sanya Research Institute of Nanjing Agricultural University, Nanjing Agricultural University, Sanya 572000, China"},{"name":"Jiangsu Livestock Embryo Engineering Laboratory, Nanjing Agricultural University, Nanjing 210095, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongxu","family":"Li","sequence":"additional","affiliation":[{"name":"Sanya Research Institute of Nanjing Agricultural University, Nanjing Agricultural University, Sanya 572000, China"},{"name":"Jiangsu Livestock Embryo Engineering Laboratory, Nanjing Agricultural University, Nanjing 210095, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiahe","family":"Guo","sequence":"additional","affiliation":[{"name":"Sanya Research Institute of Nanjing Agricultural University, Nanjing Agricultural University, Sanya 572000, China"},{"name":"Jiangsu Livestock Embryo Engineering Laboratory, Nanjing Agricultural University, Nanjing 210095, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4965-836X","authenticated-orcid":false,"given":"Feng","family":"Wang","sequence":"additional","affiliation":[{"name":"Sanya Research Institute of Nanjing Agricultural University, Nanjing Agricultural University, Sanya 572000, China"},{"name":"Jiangsu Livestock Embryo Engineering Laboratory, Nanjing Agricultural University, Nanjing 210095, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianlong","family":"Guo","sequence":"additional","affiliation":[{"name":"Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Hohhot 010030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,11,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"E588","DOI":"10.1073\/pnas.1118192109","article-title":"Structural basis for a molecular allosteric control mechanism of cofactor binding to nuclear receptors","volume":"109","author":"Osz","year":"2012","journal-title":"Proc. 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