{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T13:21:49Z","timestamp":1785331309426,"version":"3.55.0"},"reference-count":10,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2025,11,27]],"date-time":"2025-11-27T00:00:00Z","timestamp":1764201600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100020065","name":"San Antonio Medical Foundation","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100020065","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000001","name":"US National Science Foundation","doi-asserted-by":"crossref","award":["2409676"],"award-info":[{"award-number":["2409676"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>In this manuscript, we isolate and characterize a phage that displays what we call anti-bacterial hyper-aggressive behavior. This behavior appears ideal for phage therapy of bacterial disease. It includes (1) formation of semi-turbid zones that subsequently clear, (2) formation of miniature satellite plaques, which probably constitute the foundation of the semi-turbid zones, (3) multi-day enlargement of both circular plaques and cleared semi-turbid zones, and (4) non-formation of phage-resistant host colonies. We emphasize the following key details in our response. (1) The semi-turbid zones are asymmetric and occupy an area much larger (2\u201310x) than the area of circular plaques formed on the same Petri plate (unlike semi-turbid plaques associated with other phenomena, such as lysis inhibition and lysogeny). (2) In the manuscript\u2019s Figure 9d, we note that phage 0524phi7-1 destroys mature colonies of the host (unlike the behavior of other aggressive phages). (3) The asymmetry of semi-turbid zones is a point that we should have emphasized (because it implies non-diffusive, energy-requiring phage transport). (4) The input of energy for phage motion can be physical (to which we add some details for two physical effects); our mentioning of phage swimming is a hypothesis (that is, however, still viable).<\/jats:p>","DOI":"10.3390\/ijms262311468","type":"journal-article","created":{"date-parts":[[2025,11,27]],"date-time":"2025-11-27T10:25:16Z","timestamp":1764239116000},"page":"11468","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Reply to Abedon, S.T. Dual-Receptor Recognition, Lysis Inhibition, Endolysin Release, and Reaction\u2013Diffusion as Alternative Explanations. Comment on \u201cRojero et al. Bypassing Evolution of Bacterial Resistance to Phages: The Example of Hyper-Aggressive Phage 0524phi7-1. Int. J. Mol. Sci. 2025, 26, 2914\u201d"],"prefix":"10.3390","volume":"26","author":[{"given":"Maria","family":"Rojero","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Structural Biology, UT Health, San Antonio, TX 78229, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9563-0491","authenticated-orcid":false,"given":"Meagan","family":"Weaver-Rosen","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Structural Biology, UT Health, San Antonio, TX 78229, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8990-3251","authenticated-orcid":false,"given":"Philip","family":"Serwer","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Structural Biology, UT Health, San Antonio, TX 78229, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,11,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Abedon, S.T. (2025). Dual-Receptor Recognition, Lysis Inhibition, Endolysin Release, and Reaction\u2013Diffusion as Alternative Explanations. Comment on Rojero et al. Bypassing Evolution of Bacterial Resistance to Phages: The Example of Hyper-Aggressive Phage 0524phi7-1. Int. J. Mol. Sci. 2025, 26, 2914. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms262311368"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Abedon, S.T. (2019). Look who\u2019s talking: T-even phage lysis Inhibition, the granddaddy of virus-virus intercellular communication research. Viruses, 11.","DOI":"10.3390\/v11100951"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Serwer, P., Wright, E.T., De La Chapa, J., and Gonzales, C.B. (2021). Basics for improved use of phages for therapy. Antibiotics, 10.","DOI":"10.3390\/antibiotics10060723"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"8704","DOI":"10.1128\/JB.01033-07","article-title":"A wall of funnels concentrates swimming bacteria","volume":"189","author":"Galajda","year":"2007","journal-title":"J. Bacteriol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"e2411608121","DOI":"10.1073\/pnas.2411608121","article-title":"Transport and energetics of bacterial rectification","volume":"121","author":"Anand","year":"2024","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_6","unstructured":"Perrin, J. (1916). Atoms, D. Van Nostrand Company."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Ganguly, A., Alessio, B.M., and Gupta, A. (2023). Diffusiophoresis: A novel transport mechanism\u2014Fundamentals, applications, and future opportunities. Front. Sensors, 4.","DOI":"10.3389\/fsens.2023.1322906"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"620","DOI":"10.1093\/genetics\/31.6.620","article-title":"Mutation of bacteriophage with respect to type of plaque","volume":"31","author":"Hershey","year":"1946","journal-title":"Genetics"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/0022-2836(92)90711-R","article-title":"GTPase activity of bacteriophage T4 sheath protein","volume":"223","author":"Serysheva","year":"1992","journal-title":"J. Mol. Biol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1038\/emboj.2009.36","article-title":"The tail sheath structure of bacteriophage T4: A molecular machine for infecting bacteria","volume":"28","author":"Aksyuk","year":"2009","journal-title":"EMBO J."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/23\/11468\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,29]],"date-time":"2025-11-29T05:16:20Z","timestamp":1764393380000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/23\/11468"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,27]]},"references-count":10,"journal-issue":{"issue":"23","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["ijms262311468"],"URL":"https:\/\/doi.org\/10.3390\/ijms262311468","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,27]]}}}