{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T19:36:16Z","timestamp":1787859376181,"version":"build-2784847793"},"reference-count":57,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2025,11,27]],"date-time":"2025-11-27T00:00:00Z","timestamp":1764201600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["32302749"],"award-info":[{"award-number":["32302749"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"PhD Scientific Research and Innovation Foundation of Sanya Yazhou Bay Science and Technology City","award":["HSPHDSRF-2023-05-006-ZJ"],"award-info":[{"award-number":["HSPHDSRF-2023-05-006-ZJ"]}]},{"name":"Science and Technology Talent and Platform Program","award":["202505AF350008"],"award-info":[{"award-number":["202505AF350008"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Global climate change exacerbates heat stress, a major threat to male fertility in livestock. As Sertoli cells (SCs) are essential for spermatogenesis but are highly vulnerable to heat, understanding the underlying metabolic dysfunction is critical. This study investigated heat-induced metabolic dysregulation in goat SCs and evaluated the protective effects of melatonin. Through integrated transcriptomics and stable isotope-assisted metabolic flux analysis (MFA), we mapped alterations in gene expression and central carbon metabolism. Heat stress provoked mitochondrial dysfunction and oxidative damage. Metabolic flux analysis revealed suppressed mitochondrial oxidative metabolism, evidenced by reduced glucose-derived carbon incorporation into TCA cycle intermediates. Transcriptomics identified key perturbations in energy metabolism and sphingolipid signaling. Melatonin pretreatment restored mitochondrial function and redox homeostasis, and triggered a protective metabolic reprogramming that enhanced cell viability. Our findings demonstrate that melatonin mitigates heat stress by preserving mitochondrial integrity and redox homeostasis, providing mechanistic insights into male infertility and suggesting melatonin as a promising therapeutic for enhancing livestock reproductive resilience under climate stress.<\/jats:p>","DOI":"10.3390\/ijms262311475","type":"journal-article","created":{"date-parts":[[2025,11,27]],"date-time":"2025-11-27T10:25:16Z","timestamp":1764239116000},"page":"11475","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Melatonin Rescues Heat Stress-Induced Suppression of TCA Cycle and Mitochondrial Damage in Goat Sertoli Cells"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3349-2692","authenticated-orcid":false,"given":"Guang","family":"Yang","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology, Sanya Institute of China Agricultural University, Sanya 572025, China"},{"name":"State Key Laboratory of Farm Animal Biotech Breeding, Frontiers Science Center for Molecular Design Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yilin","family":"Shan","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Sanya Institute of China Agricultural University, Sanya 572025, China"},{"name":"State Key Laboratory of Farm Animal Biotech Breeding, Frontiers Science Center for Molecular Design Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Sanya Institute of China Agricultural University, Sanya 572025, China"},{"name":"State Key Laboratory of Farm Animal Biotech Breeding, Frontiers Science Center for Molecular Design Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0336-0955","authenticated-orcid":false,"given":"Hao","family":"Wu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Farm Animal Biotech Breeding, Frontiers Science Center for Molecular Design Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanyuan","family":"Lei","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Sanya Institute of China Agricultural University, Sanya 572025, China"},{"name":"State Key Laboratory of Farm Animal Biotech Breeding, Frontiers Science Center for Molecular Design Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yichong","family":"Sun","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Sanya Institute of China Agricultural University, Sanya 572025, China"},{"name":"State Key Laboratory of Farm Animal Biotech Breeding, Frontiers Science Center for Molecular Design Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pengyun","family":"Ji","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Sanya Institute of China Agricultural University, Sanya 572025, China"},{"name":"State Key Laboratory of Farm Animal Biotech Breeding, Frontiers Science Center for Molecular Design Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5688-3645","authenticated-orcid":false,"given":"Liu","family":"Guoshi","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Sanya Institute of China Agricultural University, Sanya 572025, China"},{"name":"State Key Laboratory of Farm Animal Biotech Breeding, Frontiers Science Center for Molecular Design Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,11,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"392","DOI":"10.1038\/s41586-025-09450-9","article-title":"Systematic attribution of heatwaves to the emissions of carbon majors","volume":"645","author":"Quilcaille","year":"2025","journal-title":"Nature"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"V, R.C., Johnvictor, A.C., and N, P.S. (2025). Comparative analysis of machine learning approaches for heatwave event prediction in India. Sci. Rep., 15.","DOI":"10.1038\/s41598-025-04634-9"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Wen, F., Gao, J., Zhang, G., Guo, S., Zhang, X., Han, S., Feng, X., Chen, X., and Hu, J. (2025). ROS-DRP1-mediated excessive mitochondrial fission and autophagic flux inhibition contribute to heat stress-induced apoptosis in goat Sertoli cells. J. Anim. Sci. Biotechnol., 16.","DOI":"10.1186\/s40104-025-01180-2"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"El-Sherbiny, H.R., Hashem, N.M., and Abdelnaby, E.A. (2023). Coat color affects the resilience against heat stress impacts on testicular hemodynamics, reproductive hormones, and semen quality in Baladi goats. BMC Vet. Res., 19.","DOI":"10.1186\/s12917-023-03653-w"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1093\/molehr\/gaaa002","article-title":"Melatonin protects the mouse testis against heat-induced damage","volume":"26","author":"Zhang","year":"2020","journal-title":"Mol. Hum. Reprod."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"111216","DOI":"10.1016\/j.mce.2021.111216","article-title":"Heat stress response of somatic cells in the testis","volume":"527","author":"Aldahhan","year":"2021","journal-title":"Mol. Cell Endocrinol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"s133","DOI":"10.1017\/S1751731118001167","article-title":"Review: Testicular vascular cone development and its association with scrotal thermoregulation, semen quality and sperm production in bulls","volume":"12","author":"Kastelic","year":"2018","journal-title":"Animal"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1016\/j.theriogenology.2020.08.034","article-title":"Amelioration of heat stress-induced damage to testes and sperm quality","volume":"158","author":"Shahat","year":"2020","journal-title":"Theriogenology"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/j.theriogenology.2019.03.036","article-title":"Heat stress impact on the expression patterns of different reproduction related genes in Malabari goats","volume":"131","author":"Amitha","year":"2019","journal-title":"Theriogenology"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Liu, Q.Q., Li, X., Li, J.H., Zhou, Y., Lei, M.K., Yin, W.Q., Ren, Y.S., Yang, C.H., and Zhang, C.X. (2025). Melatonin Improves Semen Quality by Modulating Oxidative Stress, Endocrine Hormones, and Tryptophan Metabolism of Hu Rams Under Summer Heat Stress and the Non-Reproductive Season. Antioxidants, 14.","DOI":"10.3390\/antiox14060630"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"107513","DOI":"10.1016\/j.celrep.2020.03.077","article-title":"Distinct Roles for Rac1 in Sertoli Cell Function during Testicular Development and Spermatogenesis","volume":"31","author":"Heinrich","year":"2020","journal-title":"Cell Rep."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.molmed.2019.09.006","article-title":"Crosstalk between Sertoli and Germ Cells in Male Fertility","volume":"26","author":"Wu","year":"2020","journal-title":"Trends Mol. Med."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1038\/s41419-017-0201-6","article-title":"Actin nucleator Spire 1 is a regulator of ectoplasmic specialization in the testis","volume":"9","author":"Wen","year":"2018","journal-title":"Cell Death Dis."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"109885","DOI":"10.1016\/j.celrep.2021.109885","article-title":"Cdc42 activity in Sertoli cells is essential for maintenance of spermatogenesis","volume":"37","author":"Heinrich","year":"2021","journal-title":"Cell Rep."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"7189","DOI":"10.1074\/jbc.M116.774471","article-title":"Quantifying intracellular rates of glycolytic and oxidative ATP production and consumption using extracellular flux measurements","volume":"292","author":"Mookerjee","year":"2017","journal-title":"J. Biol. Chem."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Yan, L., Raj, P., Yao, W., and Ying, H. (2019). Glucose Metabolism in Pancreatic Cancer. Cancers, 11.","DOI":"10.3390\/cancers11101460"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1186\/s12967-024-05182-y","article-title":"Heat stress upregulates arachidonic acid to trigger autophagy in sertoli cells via dysfunctional mitochondrial respiratory chain function","volume":"22","author":"Hu","year":"2024","journal-title":"J. Transl. Med."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"e12819","DOI":"10.1111\/jpi.12819","article-title":"Melatonin alleviates the heat stress-induced impairment of Sertoli cells by reprogramming glucose metabolism","volume":"73","author":"Deng","year":"2022","journal-title":"J. Pineal Res."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1002\/mrd.23146","article-title":"Vitamin C mitigates heat damage by reducing oxidative stress, inducing HSP expression in TM4 Sertoli cells","volume":"86","author":"Sun","year":"2019","journal-title":"Mol. Reprod. Dev."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.theriogenology.2024.04.005","article-title":"Glutamate rescues heat stress-induced apoptosis of Sertoli cells by enhancing the activity of antioxidant enzymes and activating the Trx1-Akt pathway in vitro","volume":"223","author":"Cai","year":"2024","journal-title":"Theriogenology"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Liu, D.L., Liu, S.J., Hu, S.Q., Chen, Y.C., and Guo, J. (2022). Probing the Potential Mechanism of Quercetin and Kaempferol against Heat Stress-Induced Sertoli Cell Injury: Through Integrating Network Pharmacology and Experimental Validation. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms231911163"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"100078","DOI":"10.1016\/j.cstres.2025.100078","article-title":"Melatonin ameliorates heat stress-induced oxidative apoptosis in mouse spermatocytes via autophagy and ferroptosis pathways","volume":"30","author":"Lei","year":"2025","journal-title":"Cell Stress Chaperones"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Wu, H., Qin, B., Yang, G., Ji, P., Gao, Y., Zhang, L., Wang, B., and Liu, G. (2025). The Protective Effects of Melatonin on Hainan Black Goats Under Heat Stress: Understanding Its Actions and Mechanisms. Antioxidants, 14.","DOI":"10.3390\/antiox14010044"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"9152","DOI":"10.3168\/jds.2016-11501","article-title":"Modification of embryonic resistance to heat shock in cattle by melatonin and genetic variation in HSPA1L","volume":"99","author":"Ortega","year":"2016","journal-title":"J. Dairy Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.toxlet.2024.09.003","article-title":"OSGIN1 regulates PM(2.5)-induced fibrosis via mediating autophagy in an in vitro model of COPD","volume":"401","author":"Tang","year":"2024","journal-title":"Toxicol. Lett."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Khoi, C.S., Xiao, C.Q., Hung, K.Y., Lin, T.Y., and Chiang, C.K. (2022). Oxidative Stress-Induced Growth Inhibitor (OSGIN1), a Target of X-Box-Binding Protein 1, Protects Palmitic Acid-Induced Vascular Lipotoxicity through Maintaining Autophagy. Biomedicines, 10.","DOI":"10.3390\/biomedicines10050992"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"678","DOI":"10.1038\/s41419-024-07044-3","article-title":"SPHK1 promotes bladder cancer metastasis via PD-L2\/c-Src\/FAK signaling cascade","volume":"15","author":"Kao","year":"2024","journal-title":"Cell Death Dis."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.freeradbiomed.2022.07.001","article-title":"miR-495-3p regulates sphingolipid metabolic reprogramming to induce Sphk1\/ceramide mediated mitophagy and apoptosis in NSCLC","volume":"189","author":"Arora","year":"2022","journal-title":"Free Radic. Biol. Med."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Luo, Y., Xue, H., Gao, Y., Ji, G., and Wu, T. (2025). Sphingosine kinase 2 in cancer: A review of its expression, function, and inhibitor development. Int. J. Biol. Macromol., 306.","DOI":"10.1016\/j.ijbiomac.2025.141392"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1186\/s12967-021-03066-z","article-title":"Identification of circulating sphingosine kinase-related metabolites for prediction of type 2 diabetes","volume":"19","author":"Chen","year":"2021","journal-title":"J. Transl. Med."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"3528","DOI":"10.1093\/brain\/awad033","article-title":"SMPD4 regulates mitotic nuclear envelope dynamics and its loss causes microcephaly and diabetes","volume":"146","author":"Smits","year":"2023","journal-title":"Brain"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1737","DOI":"10.1007\/s00394-017-1456-5","article-title":"Effect of GPR84 deletion on obesity and diabetes development in mice fed long chain or medium chain fatty acid rich diets","volume":"57","author":"Browne","year":"2018","journal-title":"Eur. J. Nutr."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Hellsten, S.V., Tripathi, R., Ceder, M.M., and Fredriksson, R. (2018). Nutritional Stress Induced by Amino Acid Starvation Results in Changes for Slc38 Transporters in Immortalized Hypothalamic Neuronal Cells and Primary Cortex Cells. Front. Mol. Biosci., 5.","DOI":"10.3389\/fmolb.2018.00045"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Han, X., Wang, Z., Shi, L., Wei, Z., Shangguan, J., Shi, L., and Zhao, M. (2025). Spermidine enhances the heat tolerance of Ganoderma lucidum by promoting mitochondrial respiration driven by fatty acid \u03b2-oxidation. Appl. Environ. Microbiol., 91.","DOI":"10.1128\/aem.00979-24"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"F147","DOI":"10.1530\/REP-20-0592","article-title":"REPRODUCTIVE TOXICOLOGY: Environmental exposures, fetal testis development and function: Phthalates and beyond","volume":"162","author":"Li","year":"2021","journal-title":"Reproduction"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1648","DOI":"10.1093\/biolre\/ioz053","article-title":"Formation of organotypic testicular organoids in microwell culture\u2020","volume":"100","author":"Sakib","year":"2019","journal-title":"Biol. Reprod."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Guo, X., Xu, J., Zhao, Y., Wang, J., Fu, T., Richard, M.L., Sokol, H., Wang, M., Li, Y., and Liu, Y. (2024). Melatonin alleviates heat stress-induced spermatogenesis dysfunction in male dairy goats by regulating arachidonic acid metabolism mediated by remodeling the gut microbiota. Microbiome, 12.","DOI":"10.1186\/s40168-024-01942-6"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"2200","DOI":"10.1039\/D2FO03423A","article-title":"Cyanidin-3-O-glucoside and protocatechuic acid alleviate heat stress-induced testicular damage","volume":"14","author":"Cai","year":"2023","journal-title":"Food Funct."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/j.theriogenology.2021.06.024","article-title":"Acute heat stress reduces viability but increases lactate secretion of porcine immature Sertoli cells through transcriptome reprogramming","volume":"173","author":"Yang","year":"2021","journal-title":"Theriogenology"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/j.theriogenology.2019.09.031","article-title":"Oxidative stress mediates heat-induced changes of tight junction proteins in porcine sertoli cells via inhibiting CaMKKbeta-AMPK pathway","volume":"142","author":"Yang","year":"2020","journal-title":"Theriogenology"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"2384564","DOI":"10.1080\/21505594.2024.2384564","article-title":"Porcine reproductive and respiratory syndrome virus infects the reproductive system of male piglets and impairs development of the blood-testis barrier","volume":"15","author":"Huang","year":"2024","journal-title":"Virulence"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1158\/1541-7786.MCR-18-0199","article-title":"De Novo Fatty Acid Synthesis-Driven Sphingolipid Metabolism Promotes Metastatic Potential of Colorectal Cancer","volume":"17","author":"Jafari","year":"2019","journal-title":"Mol. Cancer Res."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Wu, Y., Bai, L., Dai, X., Ba, L., Wan, J., Liang, W., Lin, H., and Fan, Z. (2025). Comparative transcriptomic analysis reveals the reactive oxygen species metabolism involving in melatonin-alleviated chilling injury in postharvest banana fruit. Plant Physiol. Biochem., 222.","DOI":"10.1016\/j.plaphy.2025.109693"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"113437","DOI":"10.1016\/j.ecoenv.2022.113437","article-title":"Melatonin alleviates cadmium toxicity and abiotic stress by promoting glandular trichome development and antioxidant capacity in Nicotiana tabacum","volume":"236","author":"Song","year":"2022","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"71","DOI":"10.3892\/mmr.2023.12958","article-title":"Melatonin attenuates high glucose-induced endothelial cell pyroptosis by activating the Nrf2 pathway to inhibit NLRP3 inflammasome activation","volume":"27","author":"Wang","year":"2023","journal-title":"Mol. Med. Rep."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.steroids.2017.08.005","article-title":"Melatonin protects chondrocytes from impairment induced by glucocorticoids via NAD(+)-dependent SIRT1","volume":"126","author":"Yang","year":"2017","journal-title":"Steroids"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"514","DOI":"10.24272\/j.issn.2095-8137.2021.041","article-title":"Melatonin relieves heat-induced spermatocyte apoptosis in mouse testes by inhibition of ATF6 and PERK signaling pathways","volume":"42","author":"Qin","year":"2021","journal-title":"Zool. Res."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1821","DOI":"10.1080\/15548627.2022.2162245","article-title":"The mitophagy receptor BNIP3 is critical for the regulation of metabolic homeostasis and mitochondrial function in the nucleus pulposus cells of the intervertebral disc","volume":"19","author":"Madhu","year":"2023","journal-title":"Autophagy"},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Li, T., Pang, N., He, L., Xu, Y., Fu, X., Tang, Y., Shachar-Hill, Y., and Chen, S. (2022). Re-Programing Glucose Catabolism in the Microalga Chlorella sorokiniana under Light Condition. Biomolecules, 12.","DOI":"10.3390\/biom12070939"},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"An, X., Li, Q., Chen, N., Li, T., Wang, H., Su, M., Shi, H., and Ma, Y. (2022). Effects of Pgam1-mediated glycolysis pathway in Sertoli cells on Spermatogonial stem cells based on transcriptomics and energy metabolomics. Front. Vet. Sci., 9.","DOI":"10.3389\/fvets.2022.992877"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"e13551","DOI":"10.1111\/cpr.13551","article-title":"Omega-3 polyunsaturated fatty acids and its metabolite 12-HEPE rescue busulfan disrupted spermatogenesis via target to GPR120","volume":"57","author":"Jing","year":"2024","journal-title":"Cell Prolif."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Signorini, C., Saso, L., Ghareghomi, S., Telkoparan-Akillilar, P., Collodel, G., and Moretti, E. (2024). Redox Homeostasis and Nrf2-Regulated Mechanisms Are Relevant to Male Infertility. Antioxidants, 13.","DOI":"10.3390\/antiox13020193"},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Shehata, A.M., Saadeldin, I.M., Tukur, H.A., and Habashy, W.S. (2020). Modulation of Heat-Shock Proteins Mediates Chicken Cell Survival against Thermal Stress. Animals, 10.","DOI":"10.3390\/ani10122407"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"5894","DOI":"10.1021\/acs.jproteome.5c00667","article-title":"Metabolic Flux Analysis Uncovers Substrate-Specific Reprogramming and ATP Deficit in CORT-Induced Depressive-like Astrocytes","volume":"24","author":"Zhao","year":"2025","journal-title":"J. Proteome Res."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"788","DOI":"10.1021\/acs.jproteome.1c00516","article-title":"Stable Isotope-Resolved Metabolomics Studies on Corticosteroid-Induced PC12 Cells: A Strategy for Evaluating Glucose Catabolism in an in Vitro Model of Depression","volume":"21","author":"Tian","year":"2022","journal-title":"J. Proteome Res."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"1536","DOI":"10.1016\/j.celrep.2016.06.102","article-title":"Distinct Metabolic States Can Support Self-Renewal and Lipogenesis in Human Pluripotent Stem Cells under Different Culture Conditions","volume":"16","author":"Zhang","year":"2016","journal-title":"Cell Rep."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"145329","DOI":"10.1016\/j.scitotenv.2021.145329","article-title":"(13)C isotope-based metabolic flux analysis revealing cellular landscape of glucose metabolism in human liver cells exposed to perfluorooctanoic acid","volume":"770","author":"Zhang","year":"2021","journal-title":"Sci. Total Environ."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/23\/11475\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,29]],"date-time":"2025-11-29T05:20:34Z","timestamp":1764393634000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/23\/11475"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,27]]},"references-count":57,"journal-issue":{"issue":"23","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["ijms262311475"],"URL":"https:\/\/doi.org\/10.3390\/ijms262311475","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,27]]}}}