{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,4]],"date-time":"2025-12-04T06:57:27Z","timestamp":1764831447125,"version":"3.46.0"},"reference-count":57,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T00:00:00Z","timestamp":1764633600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["25-44-01025"],"award-info":[{"award-number":["25-44-01025"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012190","name":"Ministry of Science and Higher Education of the Russian Federation","doi-asserted-by":"crossref","award":["122042700044-6"],"award-info":[{"award-number":["122042700044-6"]}],"id":[{"id":"10.13039\/501100012190","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Phytochrome-interacting factors (PIFs) are key transcriptional regulators of phytochrome signalling that coordinate photomorphogenesis and photosynthesis under different environmental conditions. PIFs play an important role in this regulation and act mainly as negative regulators of photomorphogenesis, but under high-intensity light (HIL), their functions can also include adaptive roles. We investigated the contribution of individual PIFs to the adaptation of the photosynthetic apparatus in wild-type A. thaliana and pif4, pif5, pif4pif5, and pif1pif3pif4pif5 mutants exposed to HIL for 0, 16, 32, or 48 h. Chlorophyll fluorescence parameters (Y(II), Fv\/Fm, NPQ), net photosynthesis (Pn), transpiration rates, stomatal conductance (gS), pigment contents and the expression of key genes were evaluated. The response of plants to HIL varied depending on the duration of exposure. After 16 h of irradiation, the greatest reductions in Pn and gS were observed in the pif4pif5 and pif1pif3pif4pif5 mutants, whereas after 48 h, the decreases were most pronounced in the pif4, pif5, and pif4pif5 mutants. After 16 h of HIL exposure, the absence of pif4 and pif5 did not substantially alter the chlorophyll fluorescence parameters. However, after 48 h, both Y(II) and Fv\/Fm were lower in these mutants than in the wild type, indicating changes in PSII functional status rather than direct reductions in photochemical quantum efficiency. At 16 h, chlorophyll levels were the highest in pif5 and WT, whereas anthocyanin and UV-absorbing pigment (UAP) levels were the highest in pif4, pif5 and WT. After 48 h, the highest levels of any pigments were detected in the WT and the pif1pif3pif4pif5 mutant. These results suggest that the accumulation of anthocyanins and UAPs under HIL is likely associated with the regulation of transcription factors, such as PIFs, de-etiolated 1 (DET1), constitutive photomorphogenic 1 (COP1), and elongated hypocotyl 5 (HY5). During prolonged HIL exposure, the absence of PIF4 and PIF5 has a critical impact on photosynthesis and the accumulation of photosynthetic pigments, whereas the simultaneous loss of PIF1, PIF3, PIF4, and PIF5 is less detrimental. This finding likely indicates opposite roles of PIF1 and PIF3 in the above-described processes, on the one hand, and PIF4 and PIF5, on the other hand, under HIL conditions.<\/jats:p>","DOI":"10.3390\/ijms262311660","type":"journal-article","created":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T08:49:22Z","timestamp":1764665362000},"page":"11660","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Involvement of Phytochrome-Interacting Factors in High-Irradiance Adaptation"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9945-5504","authenticated-orcid":false,"given":"Pavel","family":"Pashkovskiy","sequence":"first","affiliation":[{"name":"K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Moscow 127276, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-4660-8449","authenticated-orcid":false,"given":"Anna","family":"Abramova","sequence":"additional","affiliation":[{"name":"K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Moscow 127276, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8548-2261","authenticated-orcid":false,"given":"Alexandra","family":"Khudyakova","sequence":"additional","affiliation":[{"name":"Institute of Basic Biological Problems, Russian Academy of Sciences, Pushchino 142290, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5853-7834","authenticated-orcid":false,"given":"Mikhail","family":"Vereshchagin","sequence":"additional","affiliation":[{"name":"K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Moscow 127276, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vladimir","family":"Kuznetsov","sequence":"additional","affiliation":[{"name":"K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Moscow 127276, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vladimir D.","family":"Kreslavski","sequence":"additional","affiliation":[{"name":"Institute of Basic Biological Problems, Russian Academy of Sciences, Pushchino 142290, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,12,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1007\/s11099-017-0754-8","article-title":"Response of Photosynthetic Apparatus in Arabidopsis thaliana L. Mutant Deficient in Phytochrome A and B to UV-B","volume":"56","author":"Kreslavski","year":"2018","journal-title":"Photosynthetica"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1016\/j.semcdb.2019.03.007","article-title":"Plant Photoreceptors: Multi-Functional Sensory Proteins and Their Signaling Networks","volume":"Volume 92","author":"Paik","year":"2019","journal-title":"Seminars in Cell & Developmental Biology"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2221","DOI":"10.1111\/nph.70572","article-title":"Light Signal Transduction in Plants: Insights from Phytochrome Nuclear Translocation and Photobody Formation","volume":"248","author":"Chang","year":"2025","journal-title":"New Phytol."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Wu, W., Chen, L., Liang, R., Huang, S., Li, X., Huang, B., Luo, H., Zhang, M., Wang, X., and Zhu, H. (2025). The Role of Light in Regulating Plant Growth, Development and Sugar Metabolism: A Review. Front. Plant Sci., 15.","DOI":"10.3389\/fpls.2024.1507628"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"4819","DOI":"10.1093\/plcell\/koae249","article-title":"Plant Phytochrome Interactions Decode Light and Temperature Signals","volume":"36","author":"Yi","year":"2024","journal-title":"Plant Cell"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"104704","DOI":"10.1016\/j.envexpbot.2021.104704","article-title":"Photomorphogenesis in Plants: The Central Role of Phytochrome Interacting Factors (PIFs)","volume":"194","author":"Wang","year":"2022","journal-title":"Environ. Exp. Bot."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1007\/s10059-013-0135-5","article-title":"Phytochrome-Interacting Factors Have Both Shared and Distinct Biological Roles","volume":"35","author":"Jeong","year":"2013","journal-title":"Mol. Cells"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2679","DOI":"10.1105\/tpc.113.112342","article-title":"Multisite Light-Induced Phosphorylation of the Transcription Factor PIF3 Is Necessary for Both Its Rapid Degradation and Concomitant Negative Feedback Modulation of Photoreceptor phyB Levels in Arabidopsis","volume":"25","author":"Ni","year":"2013","journal-title":"Plant Cell"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1388","DOI":"10.1105\/tpc.16.00125","article-title":"PIF1-Interacting Transcription Factors and Their Binding Sequence Elements Determine the in Vivo Targeting Sites of PIF1","volume":"28","author":"Kim","year":"2016","journal-title":"Plant Cell"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"3045","DOI":"10.1105\/tpc.104.025163","article-title":"PIL5, a Phytochrome-Interacting Basic Helix-Loop-Helix Protein, Is a Key Negative Regulator of Seed Germination in Arabidopsis thaliana [W]","volume":"16","author":"Oh","year":"2004","journal-title":"Plant Cell"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1038\/nature10928","article-title":"Transcription Factor PIF4 Controls the Thermosensory Activation of Flowering","volume":"484","author":"Kumar","year":"2012","journal-title":"Nature"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1038\/nature06520","article-title":"A Molecular Framework for Light and Gibberellin Control of Cell Elongation","volume":"451","author":"Pontin","year":"2008","journal-title":"Nature"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"734","DOI":"10.1093\/mp\/sss031","article-title":"Phytochrome Signaling in Green Arabidopsis Seedlings: Impact Assessment of a Mutually Negative phyB\u2013PIF Feedback Loop","volume":"5","author":"Leivar","year":"2012","journal-title":"Mol. Plant"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"4005","DOI":"10.1038\/s41467-019-11882-7","article-title":"PIF Transcription Factors Link a Neighbor Threat Cue to Accelerated Reproduction in Arabidopsis","volume":"10","author":"Fiorucci","year":"2019","journal-title":"Nat. Commun."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1598","DOI":"10.1093\/mp\/ssu087","article-title":"Combinatorial Complexity in a Transcriptionally Centered Signaling Hub in Arabidopsis","volume":"7","author":"Pfeiffer","year":"2014","journal-title":"Mol. Plant"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Toledo-Ortiz, G., Johansson, H., Lee, K.P., Bou-Torrent, J., Stewart, K., Steel, G., Rodr\u00edguez-Concepci\u00f3n, M., and Halliday, K.J. (2014). The HY5-PIF Regulatory Module Coordinates Light and Temperature Control of Photosynthetic Gene Transcription. PLoS Genet., 10.","DOI":"10.1371\/journal.pgen.1004416"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"981","DOI":"10.1111\/j.1365-313X.2006.03021.x","article-title":"PIF3 Regulates Anthocyanin Biosynthesis in an HY5-Dependent Manner with Both Factors Directly Binding Anthocyanin Biosynthetic Gene Promoters in Arabidopsis: Functional Relationship between PIF3 and HY5","volume":"49","author":"Shin","year":"2007","journal-title":"Plant J."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"414","DOI":"10.1016\/j.molp.2020.02.001","article-title":"PHYTOCHROME-INTERACTING FACTORS Interact with the ABA Receptors PYL8 and PYL9 to Orchestrate ABA Signaling in Darkness","volume":"13","author":"Qi","year":"2020","journal-title":"Mol. Plant"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"106016","DOI":"10.1016\/j.envexpbot.2024.106016","article-title":"PIF4 and Phytohormones Signalling under Abiotic Stress","volume":"228","author":"Arya","year":"2024","journal-title":"Environ. Exp. Bot."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Li, Z.-Y., Ma, N., Zhang, F.-J., Li, L.-Z., Li, H.-J., Wang, X.-F., Zhang, Z., and You, C.-X. (2024). Functions of Phytochrome Interacting Factors (PIFs) in Adapting Plants to Biotic and Abiotic Stresses. IJMS, 25.","DOI":"10.3390\/ijms25042198"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"E6695","DOI":"10.1073\/pnas.1706226114","article-title":"PIF3 Is a Negative Regulator of the CBF Pathway and Freezing Tolerance in Arabidopsis","volume":"114","author":"Jiang","year":"2017","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Pashkovskiy, P., Kreslavski, V., Khudyakova, A., Pojidaeva, E.S., Kosobryukhov, A., Kuznetsov, V., and Allakhverdiev, S.I. (2022). Independent Responses of Photosynthesis and Plant Morphology to Alterations of PIF Proteins and Light-Dependent MicroRNA Contents in Arabidopsis Thaliana Pif Mutants Grown under Lights of Different Spectral Compositions. Cells, 11.","DOI":"10.3390\/cells11243981"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Sun, Z., Li, M., Zhou, Y., Guo, T., Liu, Y., Zhang, H., and Fang, Y. (2018). Coordinated Regulation of Arabidopsis microRNA Biogenesis and Red Light Signaling through Dicer-like 1 and Phytochrome-Interacting Factor 4. PLoS Genet., 14.","DOI":"10.1371\/journal.pgen.1007247"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Semer, J., Navr\u00e1til, M., \u0160punda, V., and \u0160troch, M. (2019). Chlorophyll Fluorescence Parameters to Assess Utilization of Excitation Energy in Photosystem II Independently of Changes in Leaf Absorption. J. Photochem. Photobiol. B Biol., 197.","DOI":"10.1016\/j.jphotobiol.2019.111535"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1007\/s11120-025-01178-x","article-title":"Revisiting the QA Model of Chlorophyll-a Fluorescence Induction: New Perspectives to Monitor the Photochemical Activity and Structural Dynamics of Photosystem II","volume":"163","author":"Garab","year":"2025","journal-title":"Photosynth. Res."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"5458","DOI":"10.1093\/jxb\/erad252","article-title":"New Foundations for the Physical Mechanism of Variable Chlorophyll a Fluorescence. Quantum Efficiency versus the Light-Adapted State of Photosystem II","volume":"74","author":"Garab","year":"2023","journal-title":"J. Exp. Bot."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/j.scienta.2017.04.035","article-title":"Phytochromes Are Key Regulators of Abiotic Stress Responses in Tomato","volume":"222","author":"Gavassi","year":"2017","journal-title":"Sci. Hortic."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/j.envexpbot.2017.05.002","article-title":"Physiological and Biochemical Responses to High Light and Temperature Stress in Plants","volume":"139","author":"Orzechowska","year":"2017","journal-title":"Environ. Exp. Bot."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Didaran, F., Kordrostami, M., Ghasemi-Soloklui, A.A., Pashkovskiy, P., Kreslavski, V., Kuznetsov, V., and Allakhverdiev, S.I. (2024). The Mechanisms of Photoinhibition and Repair in Plants under High Light Conditions and Interplay with Abiotic Stressors. J. Photochem. Photobiol. B Biol., 259.","DOI":"10.1016\/j.jphotobiol.2024.113004"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"28","DOI":"10.3390\/photochem5040028","article-title":"ROS Generation in the Light Reactions of Photosynthesis Triggers Acclimation Signaling to Environmental Stress","volume":"5","author":"Moustaka","year":"2025","journal-title":"Photochem"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1903","DOI":"10.1104\/pp.15.01935","article-title":"Nonphotochemical Chlorophyll Fluorescence Quenching: Mechanism and Effectiveness in Protecting Plants from Photodamage","volume":"170","author":"Ruban","year":"2016","journal-title":"Plant Physiol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1111\/tpj.15297","article-title":"Mechanisms Shaping the Synergism of Zeaxanthin and PsbS in Photoprotective Energy Dissipation in the Photosynthetic Apparatus of Plants","volume":"107","author":"Welc","year":"2021","journal-title":"Plant J."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"4293","DOI":"10.1093\/plcell\/koac244","article-title":"Phytochrome Interacting Factor Regulates Stomatal Aperture by Coordinating Red Light and Abscisic Acid","volume":"34","author":"Li","year":"2022","journal-title":"Plant Cell"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Hasanuzzaman, M., Bhuyan, M.H.M., Zulfiqar, F., Raza, A., Mohsin, S., Mahmud, J., Fujita, M., and Fotopoulos, V. (2020). Reactive Oxygen Species and Antioxidant Defense in Plants under Abiotic Stress: Revisiting the Crucial Role of a Universal Defense Regulator. Antioxidants, 9.","DOI":"10.3390\/antiox9080681"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Solovchenko, A. (2010). Photoprotection in Plants: Optical Screening-Based Mechanisms, Springer Science & Business Media.","DOI":"10.1007\/978-3-642-13887-4"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"11626","DOI":"10.1073\/pnas.0914428107","article-title":"Direct Regulation of Phytoene Synthase Gene Expression and Carotenoid Biosynthesis by Phytochrome-Interacting Factors","volume":"107","author":"Huq","year":"2010","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1105\/tpc.113.120857","article-title":"PIFs: Systems Integrators in Plant Development","volume":"26","author":"Leivar","year":"2014","journal-title":"Plant Cell"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1016\/j.envexpbot.2015.05.012","article-title":"Multiple Functional Roles of Anthocyanins in Plant-Environment Interactions","volume":"119","author":"Landi","year":"2015","journal-title":"Environ. Exp. Bot."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.plaphy.2013.03.014","article-title":"Functional Roles of Flavonoids in Photoprotection: New Evidence, Lessons from the Past","volume":"72","author":"Agati","year":"2013","journal-title":"Plant Physiol. Biochem."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Ponnu, J., and Hoecker, U. (2021). Illuminating the COP1\/SPA Ubiquitin Ligase: Fresh Insights Into Its Structure and Functions During Plant Photomorphogenesis. Front. Plant Sci., 12.","DOI":"10.3389\/fpls.2021.662793"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Ashikhmin, A., Bolshakov, M., Pashkovskiy, P., Vereshchagin, M., Khudyakova, A., Shirshikova, G., Kozhevnikova, A., Kosobryukhov, A., Kreslavski, V., and Kuznetsov, V. (2023). The Adaptive Role of Carotenoids and Anthocyanins in Solanum Lycopersicum Pigment Mutants under High Irradiance. Cells, 12.","DOI":"10.3390\/cells12212569"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"699","DOI":"10.1111\/j.1365-313X.2012.05033.x","article-title":"Phytochrome Interacting Factors 4 and 5 Control Seedling Growth in Changing Light Conditions by Directly Controlling Auxin Signaling","volume":"71","author":"Hornitschek","year":"2012","journal-title":"Plant J."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1105\/tpc.107.052142","article-title":"The Arabidopsis Phytochrome-Interacting Factor PIF7, Together with PIF3 and PIF4, Regulates Responses to Prolonged Red Light by Modulating phyB Levels","volume":"20","author":"Leivar","year":"2008","journal-title":"Plant Cell"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"4577","DOI":"10.1093\/jxb\/erab158","article-title":"PHYTOCHROME INTERACTING FACTORS PIF4 and PIF5 Promote Heat Stress Induced Leaf Senescence in Arabidopsis","volume":"72","author":"Li","year":"2021","journal-title":"J. Exp. Bot."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"2157","DOI":"10.1105\/tpc.106.042200","article-title":"Functional Profiling Reveals That Only a Small Number of Phytochrome-Regulated Early-Response Genes in Arabidopsis Are Necessary for Optimal Deetiolation","volume":"18","author":"Khanna","year":"2006","journal-title":"Plant Cell"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"3915","DOI":"10.1105\/tpc.107.051508","article-title":"The Basic Helix-Loop-Helix Transcription Factor PIF5 Acts on Ethylene Biosynthesis and Phytochrome Signaling by Distinct Mechanisms","volume":"19","author":"Khanna","year":"2008","journal-title":"Plant Cell"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"7660","DOI":"10.1073\/pnas.0812219106","article-title":"Phytochromes Promote Seedling Light Responses by Inhibiting Four Negatively-Acting Phytochrome-Interacting Factors","volume":"106","author":"Shin","year":"2009","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Zhang, Y., Mayba, O., Pfeiffer, A., Shi, H., Tepperman, J.M., Speed, T.P., and Quail, P.H. (2013). A Quartet of PIF bHLH Factors Provides a Transcriptionally Centered Signaling Hub That Regulates Seedling Morphogenesis through Differential Expression-Patterning of Shared Target Genes in Arabidopsis. PLoS Genet., 9.","DOI":"10.1371\/journal.pgen.1003244"},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Xiang, S., Wu, S., Zhang, H., Mou, M., Chen, Y., Li, D., Wang, H., Chen, L., and Yu, D. (2020). The PIFs Redundantly Control Plant Defense Response against Botrytis Cinerea in Arabidopsis. Plants, 9.","DOI":"10.3390\/plants9091246"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1016\/0076-6879(87)48036-1","article-title":"[34] Chlorophylls and Carotenoids: Pigments of Photosynthetic Biomembranes","volume":"Volume 148","author":"Lichtenthaler","year":"1987","journal-title":"Methods in Enzymology"},{"key":"ref_51","first-page":"201","article-title":"Complementary PS II Quantum Yields Calculated from Simple Fluorescence Parameters Measured by PAM Fluorometry and the Saturation Pulse Method","volume":"1","author":"Klughammer","year":"2008","journal-title":"PAM Appl. Notes"},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Abramova, A., Vereshchagin, M., Kulkov, L., Kreslavski, V.D., Kuznetsov, V.V., and Pashkovskiy, P. (2023). Potential Role of Phytochromes A and B and Cryptochrome 1 in the Adaptation of Solanum Lycopersicum to UV-B Radiation. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms241713142"},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Xiao, Y., Chu, L., Zhang, Y., Bian, Y., Xiao, J., and Xu, D. (2022). HY5: A Pivotal Regulator of Light-Dependent Development in Higher Plants. Front. Plant Sci., 12.","DOI":"10.3389\/fpls.2021.800989"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"963","DOI":"10.1016\/j.molp.2021.03.009","article-title":"DET1-Mediated COP1 Regulation Avoids HY5 Activity over Second-Site Gene Targets to Tune Plant Photomorphogenesis","volume":"14","author":"Bourbousse","year":"2021","journal-title":"Mol. Plant"},{"key":"ref_55","first-page":"59","article-title":"Transcriptional Feedback in Plant Growth and Defense by PIFs, BZR1, HY5, and MYC Transcription Factors","volume":"41","author":"Koene","year":"2022","journal-title":"Plant Mol. Biol. Rep."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Zhou, X., Rao, S., Wrightstone, E., Sun, T., Lui, A.C.W., Welsch, R., and Li, L. (2022). Phytoene Synthase: The Key Rate-Limiting Enzyme of Carotenoid Biosynthesis in Plants. Front. Plant Sci., 13.","DOI":"10.3389\/fpls.2022.884720"},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Gupta, P., and Hirschberg, J. (2022). The Genetic Components of a Natural Color Palette: A Comprehensive List of Carotenoid Pathway Mutations in Plants. Front. Plant Sci., 12.","DOI":"10.3389\/fpls.2021.806184"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/23\/11660\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,4]],"date-time":"2025-12-04T05:14:44Z","timestamp":1764825284000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/23\/11660"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,2]]},"references-count":57,"journal-issue":{"issue":"23","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["ijms262311660"],"URL":"https:\/\/doi.org\/10.3390\/ijms262311660","relation":{},"ISSN":["1422-0067"],"issn-type":[{"type":"electronic","value":"1422-0067"}],"subject":[],"published":{"date-parts":[[2025,12,2]]}}}