{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T18:00:17Z","timestamp":1792000817302,"version":"4.4.0"},"reference-count":36,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2025,12,11]],"date-time":"2025-12-11T00:00:00Z","timestamp":1765411200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Metropolis GZM","award":["RW\/09\/2025"],"award-info":[{"award-number":["RW\/09\/2025"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Inflammatory bowel disease (IBD), including ulcerative colitis (UC) and Crohn\u2019s disease (CD), is a group of chronic inflammatory disorders characterized by alternating episodes of flares and clinical remission, often leading to intestinal fibrosis. MicroRNAs (miRNAs) are small non-coding RNAs that regulate, among other processes, cell proliferation, inflammation, and fibrosis, all of which are crucial in IBD pathogenesis and healing. Given their role in these mechanisms, miR-103a, miR-145, and miR-191 were selected as promising candidates for IBD diagnostic biomarkers. Serum expressions of miR-103a, miR-145, and miR-191 were analyzed in 47 IBD patients and 30 healthy controls. Expressions were quantified using qPCR and normalized to miR-375-3p. All analyzed miRNAs were significantly upregulated in both UC and CD compared to healthy controls. ROC curve analysis revealed miR-103a as the most promising biomarker, with AUC = 0.893 in UC and AUC = 0.905 in CD. Moreover, miR-103a demonstrated excellent sensitivity and specificity, 89.3% and 80% in UC, and 84.2% and 83.3% in CD, respectively. miR-191 also effectively differentiated UC patients from healthy individuals (AUC = 0.848; sensitivity 89.3%; specificity 80%). Comparable results of diagnostic indicators were obtained in the CD group, however, with lower sensitivity (73.7%). miR-145 showed good ability in differentiating both UC and CD patients with high sensitivity (85.7%; 84.2%) and satisfactory specificity (66.7%; 63.3%). The obtained results indicate the promising diagnostic potential of circulating miR-103a, miR-145, and miR-191 for both UC and CD.<\/jats:p>","DOI":"10.3390\/ijms262411927","type":"journal-article","created":{"date-parts":[[2025,12,11]],"date-time":"2025-12-11T08:39:18Z","timestamp":1765442358000},"page":"11927","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Potential Role of Circulating miR-103a, miR-145 and miR-191 as Diagnostic Biomarkers for Ulcerative Colitis and Crohn\u2019s Disease"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9818-9221","authenticated-orcid":false,"given":"Aleksandra","family":"G\u00f3recka","sequence":"first","affiliation":[{"name":"Department of Clinical Chemistry and Laboratory Diagnostics, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia in Katowice, 41-200 Sosnowiec, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2504-6289","authenticated-orcid":false,"given":"Celina","family":"Kruszniewska-Rajs","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia in Katowice, 41-200 Sosnowiec, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3089-0409","authenticated-orcid":false,"given":"Joanna","family":"Gola","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia in Katowice, 41-200 Sosnowiec, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3976-9189","authenticated-orcid":false,"given":"Tomasz","family":"Roma\u0144czyk","sequence":"additional","affiliation":[{"name":"H-T. Medical Center\u2014Endotherapy, Clinic of Gastroenterology, Silesian Academy, 40-555 Katowice, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5369-8161","authenticated-orcid":false,"given":"Marcin","family":"Roma\u0144czyk","sequence":"additional","affiliation":[{"name":"H-T. Medical Center\u2014Endotherapy, Clinic of Gastroenterology, Silesian Academy, 40-555 Katowice, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2307-5884","authenticated-orcid":false,"given":"Katarzyna","family":"Komosinska-Vassev","sequence":"additional","affiliation":[{"name":"Department of Clinical Chemistry and Laboratory Diagnostics, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia in Katowice, 41-200 Sosnowiec, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,12,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/j.mayocp.2018.09.013","article-title":"Mechanisms of Disease: Inflammatory Bowel Diseases","volume":"94","author":"Ramos","year":"2019","journal-title":"Mayo Clin. 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