{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,16]],"date-time":"2025-12-16T05:16:14Z","timestamp":1765862174877,"version":"3.48.0"},"reference-count":53,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2025,12,12]],"date-time":"2025-12-12T00:00:00Z","timestamp":1765497600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004837","name":"Spanish Ministerio de Ciencia e Innovaci\u00f3n","doi-asserted-by":"crossref","award":["PID2022-136414OB-I00"],"award-info":[{"award-number":["PID2022-136414OB-I00"]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Spanish Ministry of Science and Innovation with funds from the European Union NextGenera-tionEU, from the Recovery, Transformation and Resilience Plan","award":["PRTR-C17.I1"],"award-info":[{"award-number":["PRTR-C17.I1"]}]},{"DOI":"10.13039\/501100010067","name":"Gobierno de Arag\u00f3n","doi-asserted-by":"publisher","award":["B16_23R, B45_23R and GUIA group T53_23R"],"award-info":[{"award-number":["B16_23R, B45_23R and GUIA group T53_23R"]}],"id":[{"id":"10.13039\/501100010067","id-type":"DOI","asserted-by":"publisher"}]},{"name":"CIBER Fisiopatolog\u00eda de la Obesidad y Nutrici\u00f3n","award":["CB06\/03\/1012"],"award-info":[{"award-number":["CB06\/03\/1012"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Cytochrome P450, family 7, subfamily b, polypeptide 1 (CYP7B1) is a widely expressed enzyme involved in the hydroxylation of sterols. Generated by transposon technology in zygotes, male rats lacking Cyp7b1 expression in homozygosis showed an absence of Cyp7b1 mRNA expression in the liver, small intestine, adipose tissue, and muscle. Elevated levels of 25-hydroxycholesterol were found in the liver of mutant rats. After overnight fasting, plasma triglyceride (TG) levels were increased in the homozygous rats. In agreement with this, increased hepatic secretion of very-low-density lipoprotein-TG (VLDL) in fasting rats treated with tyloxapol and decreased low-density receptor protein (LDLr) on the hepatocyte plasma membranes were observed. The decrease in LDLr was not due to decreased mRNA expression but to increased expressions of its proteases (Psck9 and Mylip). RNA sequencing identified Fasn, Igfbp2, and Pcsk9 as targets of the Cyp7b1 absence. However, the hepatic protein contents of IGFBP2 were increased in Cyp7b1-deficient rats, accompanied by a normal glucose tolerance test. HepG2 cells lacking CYP7B1 showed increased expressions of FASN and IGFBP2. These results suggest a role of CYP7B1 in the control of hepatic IGFBP2 and VLDL-TG secretion as a prediabetes sign exerted through 25-hydroxycholesterol and transcriptional or translational mechanisms depending on the species.<\/jats:p>","DOI":"10.3390\/ijms262411994","type":"journal-article","created":{"date-parts":[[2025,12,12]],"date-time":"2025-12-12T16:19:02Z","timestamp":1765556342000},"page":"11994","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The Disruption of Cyp7b1 Controls IGFBP2 and Prediabetes Exerted Through Different Hydroxycholesterol Metabolites"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8100-5596","authenticated-orcid":false,"given":"Roberto","family":"Mart\u00ednez-Beamonte","sequence":"first","affiliation":[{"name":"Departamento de Bioqu\u00edmica y Biolog\u00eda Molecular y Celular, Facultad de Veterinaria, Instituto de Investigaci\u00f3n 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Biol. Chem."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1516","DOI":"10.1111\/j.1742-4658.2011.08432.x","article-title":"Cytochrome P450s in the synthesis of cholesterol and bile acids\u2014From mouse models to human diseases","volume":"279","author":"Lorbek","year":"2012","journal-title":"FEBS J."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/j.livres.2019.05.001","article-title":"The acidic pathway of bile acid synthesis: Not just an alternative pathway","volume":"3","author":"Pandak","year":"2019","journal-title":"Liver Res."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"466","DOI":"10.1016\/j.hbpd.2023.08.009","article-title":"In vivo mouse models to study bile acid synthesis and signaling","volume":"22","author":"Bhattacharya","year":"2023","journal-title":"Hepatobiliary Pancreat. Dis. Int."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1042\/bj3550509","article-title":"cyp7b1 catalyses the 7alpha-hydroxylation of dehydroepiandrosterone and 25-hydroxycholesterol in rat prostate","volume":"355","author":"Martin","year":"2001","journal-title":"Biochem. J."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"16536","DOI":"10.1074\/jbc.M001811200","article-title":"Disruption of the oxysterol 7alpha-hydroxylase gene in mice","volume":"275","author":"Lund","year":"2000","journal-title":"J. Biol. Chem."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"23937","DOI":"10.1074\/jbc.M011564200","article-title":"Neurosteroid hydroxylase CYP7B: Vivid reporter activity in dentate gyrus of gene-targeted mice and abolition of a widespread pathway of steroid and oxysterol hydroxylation","volume":"276","author":"Rose","year":"2001","journal-title":"J. Biol. Chem."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1090","DOI":"10.1016\/j.bbalip.2010.05.011","article-title":"Effects of CYP7B1-mediated catalysis on estrogen receptor activation","volume":"1801","author":"Pettersson","year":"2010","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2814","DOI":"10.1073\/pnas.0500198102","article-title":"Early onset of puberty and early ovarian failure in CYP7B1 knockout mice","volume":"102","author":"Omoto","year":"2005","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"13589","DOI":"10.1073\/pnas.162477299","article-title":"An endocrine pathway in the prostate, ERbeta, AR, 5alpha-androstane-3beta,17beta-diol, and CYP7B1, regulates prostate growth","volume":"99","author":"Weihua","year":"2002","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2195","DOI":"10.1016\/S0022-2275(20)32094-0","article-title":"Structure and functions of human oxysterol 7alpha-hydroxylase cDNAs and gene CYP7B1","volume":"40","author":"Wu","year":"1999","journal-title":"J. Lipid Res."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1016\/j.jsbmb.2007.03.026","article-title":"Dehydroepiandrosterone 7alpha-hydroxylation in human tissues: Possible interference with type 1 11beta-hydroxysteroid dehydrogenase-mediated processes","volume":"104","author":"Hennebert","year":"2007","journal-title":"J. Steroid Biochem. Mol. Biol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.jsbmb.2005.12.001","article-title":"Inter-conversion of 7alpha- and 7beta-hydroxy-dehydroepiandrosterone by the human 11beta-hydroxysteroid dehydrogenase type 1","volume":"99","author":"Muller","year":"2006","journal-title":"J. Steroid Biochem. Mol. Biol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1111\/j.1399-0004.2011.01624.x","article-title":"Clinical phenotype variability in patients with hereditary spastic paraplegia type 5 associated with CYP7B1 mutations","volume":"81","author":"Arnoldi","year":"2012","journal-title":"Clin. Genet."},{"key":"ref_15","first-page":"710","article-title":"Hereditary spastic paraparesis due to SPG5\/CYP7B1 mutation with potential therapeutic implications","volume":"38","year":"2023","journal-title":"Neurologia"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1124\/jpet.102.033553","article-title":"Effect of hyperinsulinemia and type 2 diabetes-like hyperglycemia on expression of hepatic cytochrome p450 and glutathione s-transferase isoforms in a New Zealand obese-derived mouse backcross population","volume":"302","author":"Pass","year":"2002","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"38378","DOI":"10.1074\/jbc.M105805200","article-title":"27-hydroxycholesterol is an endogenous ligand for liver X receptor in cholesterol-loaded cells","volume":"276","author":"Fu","year":"2001","journal-title":"J. Biol. Chem."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1185","DOI":"10.1038\/nm1641","article-title":"27-Hydroxycholesterol is an endogenous SERM that inhibits the cardiovascular effects of estrogen","volume":"13","author":"Umetani","year":"2007","journal-title":"Nat. Med."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"16764","DOI":"10.1073\/pnas.0909142106","article-title":"25-Hydroxycholesterol secreted by macrophages in response to Toll-like receptor activation suppresses immunoglobulin A production","volume":"106","author":"Bauman","year":"2009","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"100486","DOI":"10.1016\/j.jlr.2023.100486","article-title":"25-Hydroxycholesterol in health and diseases","volume":"65","author":"Nguyen","year":"2024","journal-title":"J. Lipid Res."},{"key":"ref_21","first-page":"904","article-title":"Note sur l\u2019extirpation des capsules servenales chez les rats albios (Mus Rattus)","volume":"43","author":"Philipeaux","year":"1856","journal-title":"Comptes Rendus Hebd. Seances L\u2019academie Sci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/0076-6879(86)28063-5","article-title":"Comparative analysis of mammalian plasma lipoproteins","volume":"128","author":"Chapman","year":"1986","journal-title":"Methods Enzym."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1367","DOI":"10.1016\/S0022-2275(20)36966-2","article-title":"Apolipoprotein B mRNA editing in 12 different mammalian species: Hepatic expression is reflected in low concentrations of apoB-containing plasma lipoproteins","volume":"34","author":"Greeve","year":"1993","journal-title":"J. Lipid Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1637","DOI":"10.1016\/S0022-2275(20)35728-X","article-title":"Role of liver in the maintenance of cholesterol and low density lipoprotein homeostasis in different animal species, including humans","volume":"34","author":"Dietschy","year":"1993","journal-title":"J. Lipid Res."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2525","DOI":"10.1007\/s00125-006-0356-7","article-title":"Early diabetes-induced biochemical changes in the retina: Comparison of rat and mouse models","volume":"49","author":"Obrosova","year":"2006","journal-title":"Diabetologia"},{"key":"ref_26","unstructured":"(2025, May 01). NLM Genome Assembly GRCr8, Available online: https:\/\/www.ncbi.nlm.nih.gov\/datasets\/genome\/GCF_036323735.1\/."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"883","DOI":"10.1172\/JCI116663","article-title":"Hypercholesterolemia in low density lipoprotein receptor knockout mice and its reversal by adenovirus-mediated gene delivery","volume":"92","author":"Ishibashi","year":"1993","journal-title":"J. Clin. Investig."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"4431","DOI":"10.1073\/pnas.91.10.4431","article-title":"The two-receptor model of lipoprotein clearance: Tests of the hypothesis in \u201cknockout\u201d mice lacking the low density lipoprotein receptor, apolipoprotein E, or both proteins","volume":"91","author":"Ishibashi","year":"1994","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1262","DOI":"10.1172\/JCI61919","article-title":"Regulation of hepatic LDL receptors by mTORC1 and PCSK9 in mice","volume":"122","author":"Ai","year":"2012","journal-title":"J. Clin. Investig."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"100269","DOI":"10.1016\/j.jlr.2022.100269","article-title":"Associations between insulin-like growth factor binding protein-2 and lipoprotein kinetics in men","volume":"63","author":"Rauzier","year":"2022","journal-title":"J. Lipid Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1035","DOI":"10.1016\/j.bbalip.2017.07.001","article-title":"Hepatic ABCA1 deficiency is associated with delayed apolipoprotein B secretory trafficking and augmented VLDL triglyceride secretion","volume":"1862","author":"Liu","year":"2017","journal-title":"Biochim. Biophys. Acta Mol. Cell Biol. Lipids"},{"key":"ref_32","first-page":"3236","article-title":"Syndecan-1 is the primary heparan sulfate proteoglycan mediating hepatic clearance of triglyceride-rich lipoproteins in mice","volume":"119","author":"Stanford","year":"2009","journal-title":"J. Clin. Investig."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"34414","DOI":"10.1074\/jbc.M113.508812","article-title":"Differential phospholipid substrates and directional transport by ATP-binding cassette proteins ABCA1, ABCA7, and ABCA4 and disease-causing mutants","volume":"288","author":"Quazi","year":"2013","journal-title":"J. Biol. Chem."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"4812","DOI":"10.1038\/s41467-022-32437-3","article-title":"ABCA1 is an extracellular phospholipid translocase","volume":"13","author":"Segrest","year":"2022","journal-title":"Nat. Commun."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"12197","DOI":"10.1074\/jbc.M109.096933","article-title":"Targeted deletion of hepatocyte ABCA1 leads to very low density lipoprotein triglyceride overproduction and low density lipoprotein hypercatabolism","volume":"285","author":"Chung","year":"2010","journal-title":"J. Biol. Chem."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1002\/chem.201202627","article-title":"Structural and dynamic basis of human cytochrome P450 7B1: A survey of substrate selectivity and major active site access channels","volume":"19","author":"Cui","year":"2013","journal-title":"Chemistry"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"11417","DOI":"10.1016\/S0021-9258(19)78140-X","article-title":"Alteration of lipid profiles in plasma of transgenic mice expressing human lipoprotein lipase","volume":"269","author":"Liu","year":"1994","journal-title":"J. Biol. Chem."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"23610","DOI":"10.1016\/S0021-9258(17)31559-4","article-title":"Targeted disruption of the apolipoprotein C-III gene in mice results in hypotriglyceridemia and protection from postprandial hypertriglyceridemia","volume":"269","author":"Maeda","year":"1994","journal-title":"J. Biol. Chem."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1007\/s00335-007-9025-5","article-title":"Generation of rat mutants using a coat color-tagged Sleeping Beauty transposon system","volume":"18","author":"Lu","year":"2007","journal-title":"Mamm. Genome"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1016\/j.cyto.2005.03.002","article-title":"Immune-regulation of the apolipoprotein A-I\/C-III\/A-IV gene cluster in experimental inflammation","volume":"31","author":"Navarro","year":"2005","journal-title":"Cytokine"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"2368","DOI":"10.1161\/01.ATV.19.10.2368","article-title":"Low-cholesterol and high-fat diets reduce atherosclerotic lesion development in ApoE-knockout mice","volume":"19","author":"Calleja","year":"1999","journal-title":"Arter. Thromb. Vasc. Biol."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Martinez-Beamonte, R., Navarro, M.A., Acin, S., Guillen, N., Barranquero, C., Arnal, C., Surra, J., and Osada, J. (2013). Postprandial changes in high density lipoproteins in rats subjected to gavage administration of virgin olive oil. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0055231"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1016\/j.jpba.2017.12.033","article-title":"Determination of total plasma oxysterols by enzymatic hydrolysis, solid phase extraction and liquid chromatography coupled to mass-spectrometry","volume":"150","author":"Mendiara","year":"2018","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1385\/ENDO:19:3:327","article-title":"Effect of high-fat diet on body composition and hormone responses to glucose tolerance tests","volume":"19","author":"Brown","year":"2002","journal-title":"Endocrine"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1016\/j.jbiotec.2010.12.017","article-title":"Selection of reference genes for gene expression studies in rats","volume":"151","author":"Navarro","year":"2011","journal-title":"J. Biotechnol."},{"key":"ref_46","first-page":"158790","article-title":"Hepatic Synaptotagmin 1 is involved in the remodelling of liver plasma- membrane lipid composition and gene expression in male Apoe-deficient mice consuming a Western diet","volume":"1865","author":"Pastor","year":"2020","journal-title":"Biochim. Biophys. Acta Mol. Cell Biol. Lipids"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"882","DOI":"10.1016\/j.jnutbio.2008.08.003","article-title":"Apolipoprotein E determines the hepatic transcriptional profile of dietary maslinic acid in mice","volume":"20","author":"Guillen","year":"2009","journal-title":"J. Nutr. Biochem."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1258","DOI":"10.1038\/sj.gt.3300947","article-title":"Hydrodynamics-based transfection in animals by systemic administration of plasmid DNA","volume":"6","author":"Liu","year":"1999","journal-title":"Gene Ther."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1735","DOI":"10.1089\/10430349950017734","article-title":"High levels of foreign gene expression in hepatocytes after tail vein injections of naked plasmid DNA","volume":"10","author":"Zhang","year":"1999","journal-title":"Hum. Gene Ther."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"10","DOI":"10.14806\/ej.17.1.200","article-title":"Cutadapt removes adapter sequences from high-throughput sequencing reads","volume":"17","author":"Martin","year":"2011","journal-title":"EMBnet J."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Li, B., and Dewey, C.N. (2011). RSEM: Accurate transcript quantification from RNA-Seq data with or without a reference genome. BMC Bioinform., 12.","DOI":"10.1186\/1471-2105-12-323"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1093\/bioinformatics\/btp616","article-title":"edgeR: A Bioconductor package for differential expression analysis of digital gene expression data","volume":"26","author":"Robinson","year":"2010","journal-title":"Bioinformatics"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"E249","DOI":"10.1152\/ajpendo.00199.2019","article-title":"Pgc1a is responsible for the sex differences in hepatic Cidec\/Fsp27beta mRNA expression in hepatic steatosis of mice fed a Western diet","volume":"318","author":"Barranquero","year":"2020","journal-title":"Am. J. Physiol. Endocrinol. Metab."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/24\/11994\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,16]],"date-time":"2025-12-16T05:14:42Z","timestamp":1765862082000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/24\/11994"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,12]]},"references-count":53,"journal-issue":{"issue":"24","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["ijms262411994"],"URL":"https:\/\/doi.org\/10.3390\/ijms262411994","relation":{},"ISSN":["1422-0067"],"issn-type":[{"type":"electronic","value":"1422-0067"}],"subject":[],"published":{"date-parts":[[2025,12,12]]}}}