{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T08:12:04Z","timestamp":1777709524401,"version":"3.51.4"},"reference-count":133,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2025,12,15]],"date-time":"2025-12-15T00:00:00Z","timestamp":1765756800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002383","name":"Ongoing Research Funding program","doi-asserted-by":"publisher","award":["ORF-2025-431"],"award-info":[{"award-number":["ORF-2025-431"]}],"id":[{"id":"10.13039\/501100002383","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Ulcerative colitis represents a chronic inflammatory bowel disease with limited therapeutic options due to inadequate efficacy and adverse effects of current treatments. This study investigated the therapeutic potential of Beaucarnea recurvata leaf extract (BRLE) against ulcerative colitis using integrated computational and experimental approaches to address the need for safer, multi-targeted interventions. Phytochemical profiling was performed using UPLC-ESI-MS\/MS analysis. Network pharmacology and molecular docking predicted therapeutic targets and mechanisms. In vivo validation employed an acetic acid-induced ulcerative colitis rat model with BRLE treatment at 100, 200, and 400 mg\/kg doses, evaluating clinical parameters, histopathology, oxidative stress markers, inflammatory cytokines, and protein expression. UPLC-ESI-MS\/MS revealed diverse bioactive compounds including steroidal saponins, triterpenes, and flavonoids. Network pharmacology identified 24 hub targets, and molecular docking revealed strong binding affinities (\u22126.5 to \u22129.1 kcal\/mol) between BRLE compounds and inflammatory proteins including EGFR, SRC, STAT3, and AKT1. BRLE at 200 mg\/kg significantly improved disease activity, restored glutathione levels, reduced malondialdehyde, normalized IL-10 and TNF-\u03b1 levels, downregulated EGFR, SRC, STAT3, and AKT1 expression, and enhanced mucosal healing with reduced inflammatory infiltration. BRLE demonstrates significant anti-inflammatory, antioxidant, and tissue-protection effects through multi-target mechanisms, representing a promising therapeutic intervention for ulcerative colitis treatment. Further studies in chronic models, pharmacokinetic assessments, and clinical trials are needed to support its translation into therapeutic use.<\/jats:p>","DOI":"10.3390\/ijms262412053","type":"journal-article","created":{"date-parts":[[2025,12,15]],"date-time":"2025-12-15T13:32:37Z","timestamp":1765805557000},"page":"12053","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Therapeutic Potential of Beaucarnea recurvata Leaf Extract Against Ulcerative Colitis: Integrating Phytochemical Profiling, Network Pharmacology, and Experimental Validation"],"prefix":"10.3390","volume":"26","author":[{"given":"Nora","family":"Tawfeek","sequence":"first","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy, Zagazig University, Zagazig 44519, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raha","family":"Orfali","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3318-9861","authenticated-orcid":false,"given":"Shagufta","family":"Perveen","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, University of Wisconsin-Madison, Madison, WI 53706, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Safina","family":"Ghafar","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4434-2075","authenticated-orcid":false,"given":"Eman","family":"Fikry","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy, Zagazig University, Zagazig 44519, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2437-1773","authenticated-orcid":false,"given":"Mahmoud H.","family":"Elbatreek","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, Faculty of Pharmacy, Zagazig University, Zagazig 44519, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8658-1296","authenticated-orcid":false,"given":"Samar S.","family":"Elbaramawi","sequence":"additional","affiliation":[{"name":"Department of Medicinal Chemistry, Faculty of Pharmacy, Zagazig University, Zagazig 44519, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maher M.","family":"El-Domiaty","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy, Zagazig University, Zagazig 44519, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8574-9608","authenticated-orcid":false,"given":"Azza M.","family":"El-Shafae","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy, Zagazig University, Zagazig 44519, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,12,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"F1000-Faculty Rev-294","DOI":"10.12688\/f1000research.20805.1","article-title":"Ulcerative colitis: Recent advances in the understanding of disease pathogenesis","volume":"9","author":"Porter","year":"2020","journal-title":"F1000Research"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Calvez, V., Puca, P., Di Vincenzo, F., Del Gaudio, A., Bartocci, B., Murgiano, M., Iaccarino, J., Parand, E., Napolitano, D., and Pugliese, D. (2025). Novel Insights into the Pathogenesis of Inflammatory Bowel Diseases. Biomedicines, 13.","DOI":"10.3390\/biomedicines13020305"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1093\/ecco-jcc\/jjx008","article-title":"Third European evidence-based consensus on diagnosis and management of ulcerative colitis. Part 1: Definitions, diagnosis, extra-intestinal manifestations, pregnancy, cancer surveillance, surgery, and ileo-anal pouch disorders","volume":"11","author":"Magro","year":"2017","journal-title":"J. Crohn\u2019s Colitis"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"874","DOI":"10.14309\/ajg.0000000000000183","article-title":"A treat-to-target update in ulcerative colitis: A systematic review","volume":"114","author":"Ungaro","year":"2019","journal-title":"Off. J. Am. Coll. Gastroenterol.|ACG"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Davila, M.M., and Papada, E. (2023). The Role of Plant-Derived Natural Products in the Management of Inflammatory Bowel Disease-What Is the Clinical Evidence So Far?. Life, 13.","DOI":"10.3390\/life13081703"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1007\/s13659-024-00463-x","article-title":"Natural approaches for the management of ulcerative colitis: Evidence of preclinical and clinical investigations","volume":"14","author":"Subudhi","year":"2024","journal-title":"Nat. Prod. Bioprospecting"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Xu, Z., Chang, L., Xu, Z., and Chang, L. (2017). Asparagaceae. Identification and Control of Common Weeds: Volume 3, Springer.","DOI":"10.1007\/978-981-10-5403-7"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"638","DOI":"10.1111\/1750-3841.13628","article-title":"Saponin profile of wild asparagus species","volume":"82","author":"Gil","year":"2017","journal-title":"J. Food Sci."},{"key":"ref_9","first-page":"1934578X0700200120","article-title":"Steroidal saponins and sapogenins from the Agavaceae family","volume":"2","author":"Tinto","year":"2007","journal-title":"Nat. Prod. Commun."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"094","DOI":"10.7324\/JAPS.2019.90613","article-title":"C-glycosyl flavonoids-rich extract of Dipcadi erythraeum Webb & Berthel. bulbs: Phytochemical and anticancer evaluations","volume":"9","author":"Marzouk","year":"2019","journal-title":"J. Appl. Pharm. Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"129511","DOI":"10.1016\/j.ijbiomac.2024.129511","article-title":"Review of studies on polysaccharides, lignins and small molecular compounds from three Polygonatum Mill. (Asparagaceae) spp. in crude and processed states","volume":"260","author":"Zhang","year":"2024","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"235","DOI":"10.31254\/phyto.2024.13307","article-title":"Dracaena trifasciata (Prain) Mabb.\u2014Traditional use, pharmacognosy, phytochemistry and pharmacology: A comprehensive review","volume":"13","author":"Babu","year":"2024","journal-title":"J. Phytopharm."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"17778","DOI":"10.1038\/s41598-020-74440-y","article-title":"LC\u2013MS\/MS and GC\u2013MS profiling as well as the antimicrobial effect of leaves of selected Yucca species introduced to Egypt","volume":"10","author":"Basam","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_14","first-page":"6647","article-title":"Metabolomic profiling of Sansevieria trifasciata hort ex. Prain leaves and roots by HPLC-PAD-ESI\/MS and its hepatoprotective effect via activation of the NRF2\/ARE signaling pathway in an experimentally induced liver fibrosis rat model","volume":"64","author":"Raslan","year":"2021","journal-title":"Egypt. J. Chem."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Ali, D.E., Bassam, S.M., Elatrebi, S., Habiba, E.S., Allam, E.A., Omar, E.M., Ghareeb, D.A., Abdulmalek, S.A., and Abdel-Sattar, E. (2023). HR LC-MS\/MS metabolomic profiling of Yucca aloifolia fruit and the potential neuroprotective effect on rotenone-induced Parkinson\u2019s disease in rats. PLoS ONE, 18.","DOI":"10.1371\/journal.pone.0282246"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"e18046","DOI":"10.7717\/peerj.18046","article-title":"Phytochemical profiling and antioxidant activity assessment of Bellevalia pseudolongipes via liquid chromatography-high-resolution mass spectrometry","volume":"12","year":"2024","journal-title":"PeerJ"},{"key":"ref_17","first-page":"267","article-title":"Beaucarnea updated","volume":"33","author":"Walker","year":"2015","journal-title":"CactusWorld"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Walker, C. (2020). Beaucarnea Ruscaceae. Monocotyledons, Springer.","DOI":"10.1007\/978-3-662-56486-8_25"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"946","DOI":"10.1016\/j.phytochem.2011.03.004","article-title":"Steroidal saponins from the leaves of Beaucarnea recurvata","volume":"72","author":"Eskander","year":"2011","journal-title":"Phytochemistry"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1609","DOI":"10.1016\/0031-9422(96)00107-0","article-title":"Steroidal saponins from Nolina recurvata stems and their inhibitory activity on cyclic AMP phosphodiesterase","volume":"42","author":"Mimaki","year":"1996","journal-title":"Phytochemistry"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1180","DOI":"10.1248\/cpb.43.1180","article-title":"Three new cholestane bisdesmosides from Nolina recurvata stems and their inhibitory activity on cAMP phosphodiesterase and Na+\/K+ ATPase","volume":"43","author":"Takaashi","year":"1995","journal-title":"Chem. Pharm. Bull."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"2281","DOI":"10.1016\/0040-4020(94)01098-K","article-title":"Recurvosides A-E, new polyhydroxylated steroidal saponins from Nolina recurvata stems","volume":"51","author":"Takaashi","year":"1995","journal-title":"Tetrahedron"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1016\/j.indcrop.2018.08.057","article-title":"Chemical composition and cellular structure of ponytail palm (Beaucarnea recurvata) cork","volume":"124","author":"Miranda","year":"2018","journal-title":"Ind. Crops Prod."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"152","DOI":"10.47852\/bonviewMEDIN42022711","article-title":"ADME, Molecular Targets, Docking, and Dynamic Simulation Studies of Phytoconstituents of Cymbopogon citratus (DC.)","volume":"1","author":"Ajayi","year":"2024","journal-title":"Medinformatics"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"11","DOI":"10.47852\/bonviewMEDIN42023842","article-title":"In Silico Studies as Support for Natural Products Research","volume":"2","author":"Tessema","year":"2024","journal-title":"Medinformatics"},{"key":"ref_26","first-page":"203","article-title":"LC-MS\/MS analysis of sugars, alditols, and humectants in smokeless tobacco products","volume":"28","author":"Wang","year":"2019","journal-title":"Beitr. Tab. Int."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"2646","DOI":"10.1007\/s13361-017-1786-9","article-title":"New protocol based on UHPLC-MS\/MS for quantitation of metabolites in xylose-fermenting yeasts","volume":"28","author":"Campos","year":"2017","journal-title":"J. Am. Soc. Mass. Spectrom."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"e4636","DOI":"10.1002\/bmc.4636","article-title":"LC\u2013MS\/MS method with chemical derivatization for quantitation of L-threonate in human plasma","volume":"33","author":"Zhang","year":"2019","journal-title":"Biomed. Chromatogr."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"109136","DOI":"10.1016\/j.microc.2023.109136","article-title":"Determination of seventeen sugars and sugar alcohols in fruit juice samples using hydrophilic interaction liquid chromatography-tandem mass spectrometry combining response surface methodology design","volume":"193","author":"Li","year":"2023","journal-title":"Microchem. J."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"\u0160ofranko, J., Mitro, P., Laz\u00farov\u00e1, Z., P\u00e9\u010d, M.J., Bolek, T., P\u00e9\u010dov\u00e1, R., Doh\u00e1l, M., Samo\u0161, M., and Mur\u00edn, R. (2024). Application of Liquid Chromatography Coupled to Mass Spectrometry for Direct Estimation of the Total Levels of Adenosine and Its Catabolites in Human Blood. Pharmaceuticals, 17.","DOI":"10.20944\/preprints202402.0363.v1"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Mohamed, M.E., El-Shafae, A.M., Fikry, E., Elbaramawi, S.S., Elbatreek, M.H., and Tawfeek, N. (2023). Casuarina glauca branchlets\u2019 extract as a potential treatment for ulcerative colitis: Chemical composition, in silico and in vivo studies. Front. Pharmacol., 14.","DOI":"10.3389\/fphar.2023.1322181"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.foodchem.2016.02.009","article-title":"Determination of D-glucaric acid and\/or D-glucaro-1, 4-lacton in different apple varieties through hydrophilic interaction chromatography","volume":"203","author":"Xie","year":"2016","journal-title":"Food Chem."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1356","DOI":"10.1007\/s13197-012-0643-x","article-title":"In vitro antioxidant activity of feruloyl arabinose isolated from maize bran by acid hydrolysis","volume":"51","author":"Lin","year":"2014","journal-title":"J. Food Sci. Technol."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Mohamed, M.E., Tawfeek, N., Elbaramawi, S.S., Elbatreek, M.H., and Fikry, E. (2022). Agathis robusta bark extract protects from renal ischemia-reperfusion injury: Phytochemical, in silico and in vivo studies. Pharmaceuticals, 15.","DOI":"10.3390\/ph15101270"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Shigematsu, Y., Yuasa, M., Ishige, N., Nakajima, H., and Tajima, G. (2021). Development of second-tier liquid chromatography-tandem mass spectrometry analysis for expanded newborn screening in Japan. Int. J. Neonatal Screen., 7.","DOI":"10.3390\/ijns7030044"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Tawfeek, N., Sobeh, M., Hamdan, D.I., Farrag, N., Roxo, M., El-Shazly, A.M., and Wink, M. (2019). Phenolic compounds from Populus alba L. and Salix subserrata Willd. (Salicaceae) counteract oxidative stress in Caenorhabditis elegans. Molecules, 24.","DOI":"10.3390\/molecules24101999"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.jpba.2017.08.015","article-title":"Bioanalysis of monomethyl fumarate in human plasma by a sensitive and rapid LC-MS\/MS method and its pharmacokinetic application LC-MS\/MS determination of monomethyl fumarate in human plasma","volume":"146","author":"Varanasi","year":"2017","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1016\/j.jpba.2016.05.039","article-title":"Validation of UHPLC\u2013MS\/MS methods for the determination of kaempferol and its metabolite 4-hydroxyphenyl acetic acid, and application to in vitro blood-brain barrier and intestinal drug permeability studies","volume":"128","author":"Oufir","year":"2016","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"390","DOI":"10.1016\/j.foodchem.2019.05.068","article-title":"Identification and quantification of new isomers of isopropyl-malic acid in wine by LC-IT and LC-Q-Orbitrap","volume":"294","author":"Ricciutelli","year":"2019","journal-title":"Food Chem."},{"key":"ref_40","first-page":"100915","article-title":"Urinary metabolomic analysis of three fundamental traditional Mongolian Medicine Constitution types using UHPLC-QTOF-MS and nuclear magnetic resonance techniques","volume":"17","author":"Wu","year":"2024","journal-title":"J. Radiat. Res. Appl. Sci."},{"key":"ref_41","first-page":"2175","article-title":"UPLC-QTOF\/MS-assisted chemical profiling of Daucus carota leaf extract and evaluation of its antioxidant, antimicrobial and antibiofilm activities: Evidence from in vitro and in silico studies","volume":"66","author":"Mahmoud","year":"2023","journal-title":"Egypt. J. Chem."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"22765","DOI":"10.1038\/s41598-024-72153-0","article-title":"A comparative UPLC-orbitrap-MS-based metabolite profiling of three Pelargonium species cultivated in Egypt","volume":"14","author":"Merghany","year":"2024","journal-title":"Sci. Rep."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"856","DOI":"10.1016\/j.indcrop.2016.09.065","article-title":"Phenolic profiles and antimicrobial activity of various plant resins as potential botanical sources of Serbian propolis","volume":"94","author":"Janakiev","year":"2016","journal-title":"Ind. Crops Prod."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"104232","DOI":"10.1016\/j.arabjc.2022.104232","article-title":"LC-MS\/MS based metabolite profiling and lipase enzyme inhibitory activity of Kaempferia angustifolia Rosc. with different extracting solvents","volume":"15","author":"Rafi","year":"2022","journal-title":"Arab. J. Chem."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Markowska, P., Procaj\u0142o, Z., Wolska, J., Jaroszewski, J.J., and Zi\u00f3\u0142kowski, H. (2021). Development, Validation, and Application of the LC-MS\/MS Method for Determination of 4-Acetamidobenzoic Acid in Pharmacokinetic Pilot Studies in Pigs. Molecules, 26.","DOI":"10.3390\/molecules26154437"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1095","DOI":"10.1002\/rcm.7202","article-title":"Structural characterization of flavonoid C-and O-glycosides in an extract of Adhatoda vasica leaves by liquid chromatography with quadrupole time-of-flight mass spectrometry","volume":"29","author":"Singh","year":"2015","journal-title":"Rapid Commun. Mass. Spectrom."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Bonte, R., Bongaerts, M., Demirdas, S., Langendonk, J.G., Huidekoper, H.H., Williams, M., Onkenhout, W., Jacobs, E.H., Blom, H.J., and Ruijter, G.J. (2019). Untargeted metabolomics-based screening method for inborn errors of metabolism using semi-automatic sample preparation with an UHPLC-orbitrap-MS platform. Metabolites, 9.","DOI":"10.3390\/metabo9120289"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1878","DOI":"10.1139\/v05-214","article-title":"Fragmentation pathways of negative ions produced by electrospray ionization of acyclic dicarboxylic acids and derivatives","volume":"83","author":"Grossert","year":"2005","journal-title":"Can. J. Chem."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Schwarz, M., Weber, F., Dur\u00e1n-Guerrero, E., Castro, R., Rodr\u00edguez-Dodero, M.d.C., Garc\u00eda-Moreno, M.V., Winterhalter, P., and Guill\u00e9n-S\u00e1nchez, D. (2021). HPLC-DAD-MS and antioxidant profile of fractions from amontillado sherry wine obtained using high-speed counter-current chromatography. Foods, 10.","DOI":"10.3390\/foods10010131"},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Tine, Y., Renucci, F., Costa, J., W\u00e9l\u00e9, A., and Paolini, J. (2017). A method for LC-MS\/MS profiling of coumarins in Zanthoxylum zanthoxyloides (Lam.) B. Zepernich and Timler extracts and essential oils. Molecules, 22.","DOI":"10.3390\/molecules22010174"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"9768","DOI":"10.1021\/acs.jafc.3c06309","article-title":"Analysis of Lemon Verbena Polyphenol Metabolome and Its Correlation with Oxidative Stress under Glucotoxic Conditions in Adipocyte","volume":"72","author":"Micol","year":"2024","journal-title":"J. Agric. Food Chem."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"464084","DOI":"10.1016\/j.chroma.2023.464084","article-title":"Isotope-coded derivatization with designed Girard-type reagent as charged isobaric mass tags for non-targeted profiling and discovery of natural aldehydes by liquid chromatography-tandem mass spectrometry","volume":"1702","author":"Huang","year":"2023","journal-title":"J. Chromatogr. A"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"5233","DOI":"10.1038\/s41598-023-32224-0","article-title":"Metabolic profiling, antioxidant, and enzyme inhibition potential of Iris pseudacorus L. from Egypt and Japan: A comparative study","volume":"13","author":"Yehia","year":"2023","journal-title":"Sci. Rep."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Ali, A., Cottrell, J.J., and Dunshea, F.R. (2022). Lc-ms\/ms characterization of phenolic metabolites and their antioxidant activities from australian native plants. Metabolites, 12.","DOI":"10.3390\/metabo12111016"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"887","DOI":"10.1007\/s00216-009-3232-8","article-title":"Phenolic characterization of Northeast Portuguese propolis: Usual and unusual compounds","volume":"396","author":"Estevinho","year":"2010","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1016\/j.jfca.2017.08.003","article-title":"LC\u2013MSn characterization of saponins in mate (Ilex paraguariens, St. Hil) and their quantification by HPLC-DAD","volume":"63","author":"Mateos","year":"2017","journal-title":"J. Food Compos. Anal."},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Wu, F., Lai, S., Feng, H., Liu, J., Fu, D., Wang, C., Wang, C., Liu, J., Li, Z., and Li, P. (2022). Protective effects of protopanaxatriol saponins on ulcerative colitis in mouse based on UPLC-Q\/TOF-MS serum and colon metabolomics. Molecules, 27.","DOI":"10.3390\/molecules27238346"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"153842","DOI":"10.1016\/j.phymed.2021.153842","article-title":"Anti-inflammatory and modulatory effects of steroidal saponins and sapogenins on cytokines: A review of pre-clinical research","volume":"96","author":"Passos","year":"2022","journal-title":"Phytomedicine"},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Culhuac, E.B., Maggiolino, A., Elghandour, M.M., De Palo, P., and Salem, A.Z. (2023). Antioxidant and anti-inflammatory properties of phytochemicals found in the yucca genus. Antioxidants, 12.","DOI":"10.3390\/antiox12030574"},{"key":"ref_60","first-page":"362","article-title":"The impact of saponins on health-review","volume":"13","author":"Bouzier","year":"2023","journal-title":"Biointerface Res. Appl. Chem."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"4779","DOI":"10.3892\/mmr.2016.5094","article-title":"Ursolic acid protects against ulcerative colitis via anti-inflammatory and antioxidant effects in mice","volume":"13","author":"Liu","year":"2016","journal-title":"Mol. Med. Rep."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1142\/S0192415X21500191","article-title":"Research advances in protective effects of ursolic acid and oleanolic acid against gastrointestinal diseases","volume":"49","author":"Shi","year":"2021","journal-title":"Am. J. Chin. Med."},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Niu, Y., Zhang, J., Shi, D., Zang, W., and Niu, J. (2023). Glycosides as Potential Medicinal Components for Ulcerative Colitis: A Review. Molecules, 28.","DOI":"10.3390\/molecules28135210"},{"key":"ref_64","first-page":"627","article-title":"Anti-inflammatory effect of luteolin-7-O-glucoside via the JAK1\/STAT6\/SOCS1 pathway in ulcerative colitis treatment","volume":"18","author":"Sun","year":"2022","journal-title":"Pharmacogn. Mag."},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Li, C., Tang, Y., Ye, Y., Zuo, M., and Lu, Q. (2023). Potential of natural flavonols and flavanones in the treatment of ulcerative colitis. Front. Pharmacol., 14.","DOI":"10.3389\/fphar.2023.1120616"},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Ginsberg, A. (2012). 4-Amino Salicylic Acid in the Treatment of Ulcerative Colitis: Preliminary Report of a Randomized) Double-Blind Placebo Controlled Trial of Oral 4-ASA. Inflammatory Bowel Diseases 1990: Proceedings of the Third International Symposium on Inflammatory Bowel Diseases, Jerusalem, September 10\u201313, 1989, Springer.","DOI":"10.1007\/978-94-009-1980-8_22"},{"key":"ref_67","first-page":"196","article-title":"The role of aminosalicylates in the treatment of ulcerative colitis","volume":"65","author":"Baert","year":"2002","journal-title":"Acta Gastro-Enterol. Belg."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"2157","DOI":"10.2147\/DDDT.S499284","article-title":"Caffeic Acid Protects Against Ulcerative Colitis via Inhibiting Mitochondrial Apoptosis and Immune Overactivation in Drosophila","volume":"19","author":"Xiu","year":"2025","journal-title":"Drug Des. Dev. Ther."},{"key":"ref_69","doi-asserted-by":"crossref","unstructured":"Zieli\u0144ska, D., Zieli\u0144ski, H., Laparra-Llopis, J.M., Szawara-Nowak, D., Honke, J., and Gim\u00e9nez-Bastida, J.A. (2021). Caffeic acid modulates processes associated with intestinal inflammation. Nutrients, 13.","DOI":"10.3390\/nu13020554"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"102790","DOI":"10.1016\/j.bcab.2023.102790","article-title":"Implications of Asparagus racemosus and Terminalia chebula extracts on oxazolone induced inflammatory bowel disease in Danio rerio (zebrafish)","volume":"51","author":"Kishore","year":"2023","journal-title":"Biocatal. Agric. Biotechnol."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1080\/J157v06n01_02","article-title":"Antisecretory and antiulcer activity of Asparagus racemosus Willd. against indomethacin plus pyloric ligation-induced gastric ulcer in rats","volume":"6","author":"Bhatnagar","year":"2006","journal-title":"J. Herb. Pharmacother."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"142452","DOI":"10.1016\/j.ijbiomac.2025.142452","article-title":"Polysaccharides from Cistanche deserticola mitigate inflammatory bowel disease via modulating intestinal microbiota and SRC\/EGFR\/PI3K\/AKT signaling pathways","volume":"308","author":"Qiao","year":"2025","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"1015","DOI":"10.1007\/s13204-024-03063-w","article-title":"ZnO mesoscale nanoparticles photoluminescence obtained by green synthesis based on Beaucarnea gracilis","volume":"14","year":"2024","journal-title":"Appl. Nanosci."},{"key":"ref_74","doi-asserted-by":"crossref","unstructured":"Ren, J., Yue, B., Wang, H., Zhang, B., Luo, X., Yu, Z., Zhang, J., Ren, Y., Mani, S., and Wang, Z. (2020). Acacetin Ameliorates Experimental Colitis in Mice via Inhibiting Macrophage Inflammatory Response and Regulating the Composition of Gut Microbiota. Front. Physiol., 11.","DOI":"10.3389\/fphys.2020.577237"},{"key":"ref_75","doi-asserted-by":"crossref","unstructured":"Hu, L., Wu, C., Zhang, Z., Liu, M., Maruthi Prasad, E., Chen, Y., and Wang, K. (2019). Pinocembrin Protects Against Dextran Sulfate Sodium-Induced Rats Colitis by Ameliorating Inflammation, Improving Barrier Function and Modulating Gut Microbiota. Front. Physiol., 10.","DOI":"10.3389\/fphys.2019.00908"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"BSR20200986","DOI":"10.1042\/BSR20200986","article-title":"Pinocembrin alleviates ulcerative colitis in mice via regulating gut microbiota, suppressing TLR4\/MD2\/NF-kappaB pathway and promoting intestinal barrier","volume":"40","author":"Yue","year":"2020","journal-title":"Biosci. Rep."},{"key":"ref_77","doi-asserted-by":"crossref","unstructured":"Wang, J., Yao, Y., Yao, T., Shi, Q., Zeng, Y., and Li, L. (2024). Hesperetin Alleviated Experimental Colitis via Regulating Ferroptosis and Gut Microbiota. Nutrients, 16.","DOI":"10.3390\/nu16142343"},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"e70229","DOI":"10.1002\/jbt.70229","article-title":"Unraveling the Protective Effect of Hesperetin In Experimentally Induced Colitis: Inhibition of NF-kappaB and NLRP3 Inflammasome Activation","volume":"39","author":"Mohanad","year":"2025","journal-title":"J. Biochem. Mol. Toxicol."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"172992","DOI":"10.1016\/j.ejphar.2020.172992","article-title":"Hesperetin ameliorates DSS-induced colitis by maintaining the epithelial barrier via blocking RIPK3\/MLKL necroptosis signaling","volume":"873","author":"Zhang","year":"2020","journal-title":"Eur. J. Pharmacol."},{"key":"ref_80","doi-asserted-by":"crossref","unstructured":"Zheng, C., Rangsinth, P., Shiu, P.H.T., Wang, W., Li, R., Li, J., Kwan, Y.W., and Leung, G.P.H. (2023). A Review on the Sources, Structures, and Pharmacological Activities of Lucidenic Acids. Molecules, 28.","DOI":"10.3390\/molecules28041756"},{"key":"ref_81","doi-asserted-by":"crossref","unstructured":"Alrumaihi, F., Almatroodi, S.A., Alharbi, H.O.A., Alwanian, W.M., Alharbi, F.A., Almatroudi, A., and Rahmani, A.H. (2024). Pharmacological Potential of Kaempferol, a Flavonoid in the Management of Pathogenesis via Modulation of Inflammation and Other Biological Activities. Molecules, 29.","DOI":"10.3390\/molecules29092007"},{"key":"ref_82","doi-asserted-by":"crossref","unstructured":"Jan, R., Khan, M., Asaf, S., Asif, S., and Kim, K.M. (2022). Bioactivity and Therapeutic Potential of Kaempferol and Quercetin: New Insights for Plant and Human Health. Plants, 11.","DOI":"10.3390\/plants11192623"},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"2065","DOI":"10.1016\/S0006-2952(03)00203-X","article-title":"Novel anti-inflammatory actions of nobiletin, a citrus polymethoxy flavonoid, on human synovial fibroblasts and mouse macrophages","volume":"65","author":"Lin","year":"2003","journal-title":"Biochem. Pharmacol."},{"key":"ref_84","doi-asserted-by":"crossref","unstructured":"Ayeleso, T.B., Matumba, M.G., and Mukwevho, E. (2017). Oleanolic Acid and Its Derivatives: Biological Activities and Therapeutic Potential in Chronic Diseases. Molecules, 22.","DOI":"10.3390\/molecules22111915"},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"8239","DOI":"10.1039\/D0RA09117K","article-title":"Potential therapeutic targets and molecular details of anthocyan-treated inflammatory bowel disease: A systematic bioinformatics analysis of network pharmacology","volume":"11","author":"Lin","year":"2021","journal-title":"RSC Adv."},{"key":"ref_86","doi-asserted-by":"crossref","unstructured":"Kasembeli, M.M., Bharadwaj, U., Robinson, P., and Tweardy, D.J. (2018). Contribution of STAT3 to Inflammatory and Fibrotic Diseases and Prospects for its Targeting for Treatment. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19082299"},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"81","DOI":"10.2967\/jnmt.117.199588","article-title":"Pharmacology, part 1: Introduction to pharmacology and pharmacodynamics","volume":"46","author":"Currie","year":"2018","journal-title":"J. Nucl. Med. Technol."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/j.jsps.2016.07.002","article-title":"An overview of pharmacodynamic modelling, ligand-binding approach and its application in clinical practice","volume":"25","author":"Salahudeen","year":"2017","journal-title":"Saudi Pharm. J."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"2901","DOI":"10.1007\/s10787-023-01369-9","article-title":"Recent advances in anti-inflammatory active components and action mechanisms of natural medicines","volume":"31","author":"Wu","year":"2023","journal-title":"Inflammopharmacology"},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"1127","DOI":"10.1007\/s12272-015-0553-3","article-title":"PF2405, standardized fraction of Scutellaria baicalensis, ameliorates colitis in vitro and in vivo","volume":"38","author":"Jiang","year":"2015","journal-title":"Arch. Pharmacal Res."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"1495","DOI":"10.1038\/s41401-021-00781-7","article-title":"Baicalein ameliorates ulcerative colitis by improving intestinal epithelial barrier via AhR\/IL-22 pathway in ILC3s","volume":"43","author":"Li","year":"2022","journal-title":"Acta Pharmacol. Sin."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"1972","DOI":"10.1007\/s00044-023-03116-9","article-title":"Glutathione and glutathione disulfide\u2014Their biomedical and pharmaceutical applications","volume":"32","author":"Guan","year":"2023","journal-title":"Med. Chem. Res."},{"key":"ref_93","doi-asserted-by":"crossref","unstructured":"Senthilkumar, M., Amaresan, N., and Sankaranarayanan, A. (2021). Estimation of Malondialdehyde (MDA) by Thiobarbituric Acid (TBA) Assay. Plant-Microbe Interactions: Laboratory Techniques, Springer.","DOI":"10.1007\/978-1-0716-1080-0"},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"753","DOI":"10.1038\/s41575-022-00658-y","article-title":"The metabolic nature of inflammatory bowel diseases","volume":"19","author":"Adolph","year":"2022","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"key":"ref_95","doi-asserted-by":"crossref","unstructured":"Yoshimoto, T., and Yoshimoto, T. (2014). Interleukin-10: Cytokines in Anti-inflammation and Tolerance. Cytokine Frontiers: Regulation of Immune Responses in Health and Disease, Springer.","DOI":"10.1007\/978-4-431-54442-5"},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"628","DOI":"10.1038\/s41586-024-07098-5","article-title":"IL-10 constrains sphingolipid metabolism to limit inflammation","volume":"627","author":"York","year":"2024","journal-title":"Nature"},{"key":"ref_97","unstructured":"Rajendram, R., Preedy, V.R., and Patel, V.B. (2023). Tumor Necrosis Factor-Alpha (TNF-Alpha) as a Biomarker in Trauma and Critical Care. Biomarkers in Trauma, Injury and Critical Care, Springer International Publishing."},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1038\/ncb1086","article-title":"A physical and functional map of the human TNF-\u03b1\/NF-\u03baB signal transduction pathway","volume":"6","author":"Bouwmeester","year":"2004","journal-title":"Nat. Cell Biol."},{"key":"ref_99","doi-asserted-by":"crossref","unstructured":"Zahid Balouch, F.K. (2022). Epidermal Growth Factor (EGF) in Wound Repair. Therapeutic Proteins Against Human Diseases, Springer Nature.","DOI":"10.1007\/978-981-16-7897-4"},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1038\/s41580-024-00715-1","article-title":"Cellular and molecular mechanisms of skin wound healing","volume":"25","author":"Martin","year":"2024","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1186\/s12929-025-01119-9","article-title":"EGF receptor in organ development, tissue homeostasis and regeneration","volume":"32","author":"Tito","year":"2025","journal-title":"J. Biomed. Sci."},{"key":"ref_102","doi-asserted-by":"crossref","first-page":"512926","DOI":"10.1155\/2012\/512926","article-title":"The role of Src kinase in macrophage-mediated inflammatory responses","volume":"2012","author":"Byeon","year":"2012","journal-title":"Mediat. Inflamm."},{"key":"ref_103","doi-asserted-by":"crossref","first-page":"4591","DOI":"10.1111\/jcmm.17489","article-title":"STAT3 regulates inflammatory cytokine production downstream of TNFR1 by inducing expression of TNFAIP3\/A20","volume":"26","author":"Antonia","year":"2022","journal-title":"J. Cell Mol. Med."},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1111\/j.1749-6632.2009.04911.x","article-title":"Signal transducer and activator of transcription-3, inflammation, and cancer: How intimate is the relationship?","volume":"1171","author":"Aggarwal","year":"2009","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"ref_105","doi-asserted-by":"crossref","first-page":"1303","DOI":"10.1038\/s41598-018-19689-0","article-title":"Defining the Akt1 interactome and its role in regulating the cell cycle","volume":"8","author":"Duggal","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_106","doi-asserted-by":"crossref","first-page":"5443","DOI":"10.1007\/s11030-025-11132-7","article-title":"Structural insights of AKT and its activation mechanism for drug development","volume":"29","author":"Kumar","year":"2025","journal-title":"Mol. Divers."},{"key":"ref_107","doi-asserted-by":"crossref","first-page":"42717","DOI":"10.1038\/srep42717","article-title":"SwissADME: A free web tool to evaluate pharmacokinetics, drug-likeness and medicinal chemistry friendliness of small molecules","volume":"7","author":"Daina","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_108","doi-asserted-by":"crossref","unstructured":"Fikry, E., Orfali, R., Tawfeek, N., Perveen, S., Ghafar, S., El-Domiaty, M.M., and El-Shafae, A.M. (2024). Unveiling the Bioactive Efficacy of Cupressus sempervirens \u2018Stricta\u2019 Essential Oil: Composition, In Vitro Activities, and In Silico Analyses. Pharmaceuticals, 17.","DOI":"10.3390\/ph17081019"},{"key":"ref_109","doi-asserted-by":"crossref","first-page":"W357","DOI":"10.1093\/nar\/gkz382","article-title":"SwissTargetPrediction: Updated data and new features for efficient prediction of protein targets of small molecules","volume":"47","author":"Daina","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"ref_110","doi-asserted-by":"crossref","first-page":"e697","DOI":"10.1002\/cpz1.697","article-title":"UniProt tools: BLAST, align, peptide search, and ID mapping","volume":"3","author":"Zaru","year":"2023","journal-title":"Curr. Protoc."},{"key":"ref_111","doi-asserted-by":"crossref","first-page":"1.30.1","DOI":"10.1002\/cpbi.5","article-title":"The GeneCards Suite: From Gene Data Mining to Disease Genome Sequence Analyses","volume":"54","author":"Stelzer","year":"2016","journal-title":"Curr. Protoc. Bioinform."},{"key":"ref_112","first-page":"D845","article-title":"The DisGeNET knowledge platform for disease genomics: 2019 update","volume":"48","author":"Ronzano","year":"2020","journal-title":"Nucleic Acids Res."},{"key":"ref_113","doi-asserted-by":"crossref","first-page":"1.2.1","DOI":"10.1002\/cpbi.27","article-title":"Searching Online Mendelian Inheritance in Man (OMIM): A Knowledgebase of Human Genes and Genetic Phenotypes","volume":"58","author":"Amberger","year":"2017","journal-title":"Curr. Protoc. Bioinform."},{"key":"ref_114","doi-asserted-by":"crossref","first-page":"D638","DOI":"10.1093\/nar\/gkac1000","article-title":"The STRING database in 2023: Protein\u2013protein association networks and functional enrichment analyses for any sequenced genome of interest","volume":"51","author":"Szklarczyk","year":"2023","journal-title":"Nucleic Acids Res."},{"key":"ref_115","doi-asserted-by":"crossref","first-page":"2498","DOI":"10.1101\/gr.1239303","article-title":"Cytoscape: A software environment for integrated models of biomolecular interaction networks","volume":"13","author":"Shannon","year":"2003","journal-title":"Genome Res."},{"key":"ref_116","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.biosystems.2014.11.005","article-title":"CytoNCA: A cytoscape plugin for centrality analysis and evaluation of protein interaction networks","volume":"127","author":"Tang","year":"2015","journal-title":"Biosystems"},{"key":"ref_117","doi-asserted-by":"crossref","unstructured":"Fikry, E., Orfali, R., Perveen, S., Ghaffar, S., El-Shafae, A.M., El-Domiaty, M.M., and Tawfeek, N. (2025). Chemical Composition and Anti-Lung Cancer Activities of Melaleuca quinquenervia Leaf Essential Oil: Integrating Gas Chromatography\u2013Mass Spectrometry (GC\/MS) Profiling, Network Pharmacology, and Molecular Docking. Pharmaceuticals, 18.","DOI":"10.3390\/ph18060771"},{"key":"ref_118","doi-asserted-by":"crossref","unstructured":"Chin, C.-H., Chen, S.-H., Wu, H.-H., Ho, C.-W., Ko, M.-T., and Lin, C.-Y. (2014). cytoHubba: Identifying hub objects and sub-networks from complex interactome. BMC Syst. Biol., 8.","DOI":"10.1186\/1752-0509-8-S4-S11"},{"key":"ref_119","doi-asserted-by":"crossref","first-page":"R60","DOI":"10.1186\/gb-2003-4-9-r60","article-title":"DAVID: Database for annotation, visualization, and integrated discovery","volume":"4","author":"Dennis","year":"2003","journal-title":"Genome Biol."},{"key":"ref_120","doi-asserted-by":"crossref","unstructured":"Wlodawer, A., Dauter, Z., and Jaskolski, M. (2017). Protein Data Bank (PDB): The Single Global Macromolecular Structure Archive. Protein Crystallography: Methods and Protocols, Springer.","DOI":"10.1007\/978-1-4939-7000-1"},{"key":"ref_121","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1021\/jacs.6b08536","article-title":"Broad-Spectrum Kinase Profiling in Live Cells with Lysine-Targeted Sulfonyl Fluoride Probes","volume":"139","author":"Zhao","year":"2017","journal-title":"J. Am. Chem. Soc."},{"key":"ref_122","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1038\/nchembio.1404","article-title":"A conserved water-mediated hydrogen bond network defines bosutinib\u2019s kinase selectivity","volume":"10","author":"Levinson","year":"2014","journal-title":"Nat. Chem. Biol."},{"key":"ref_123","doi-asserted-by":"crossref","first-page":"1343","DOI":"10.1016\/j.bbagen.2019.05.019","article-title":"Unexpected implications of STAT3 acetylation revealed by genetic encoding of acetyl-lysine","volume":"1863","author":"Belo","year":"2019","journal-title":"Biochim. Biophys. Acta Gen. Subj."},{"key":"ref_124","doi-asserted-by":"crossref","first-page":"ra37","DOI":"10.1126\/scisignal.2002618","article-title":"An ATP-site on-off switch that restricts phosphatase accessibility of Akt","volume":"5","author":"Lin","year":"2012","journal-title":"Sci. Signal"},{"key":"ref_125","doi-asserted-by":"crossref","first-page":"1605","DOI":"10.1002\/jcc.20084","article-title":"UCSF Chimera\u2014A visualization system for exploratory research and analysis","volume":"25","author":"Pettersen","year":"2004","journal-title":"J. Comput. Chem."},{"key":"ref_126","doi-asserted-by":"crossref","unstructured":"Fikry, E., Orfali, R., El-Sayed, S.S., Perveen, S., Ghafar, S., El-Shafae, A.M., El-Domiaty, M.M., and Tawfeek, N. (2023). Potential Hepatoprotective Effects of Chamaecyparis lawsoniana against Methotrexate-Induced Liver Injury: Integrated Phytochemical Profiling, Target Network Analysis, and Experimental Validation. Antioxidants, 12.","DOI":"10.3390\/antiox12122118"},{"key":"ref_127","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1186\/1758-2946-3-33","article-title":"Open Babel: An open chemical toolbox","volume":"3","author":"Banck","year":"2011","journal-title":"J. Cheminform"},{"key":"ref_128","doi-asserted-by":"crossref","first-page":"W363","DOI":"10.1093\/nar\/gky473","article-title":"CASTp 3.0: Computed atlas of surface topography of proteins","volume":"46","author":"Tian","year":"2018","journal-title":"Nucleic Acids Res."},{"key":"ref_129","unstructured":"Biovia, D.S. (2021). Discovery Studio Visualizer, v21. 1.0. 20298, Dassault Syst\u00e8mes."},{"key":"ref_130","first-page":"238","article-title":"Clinicopathologic study of dextran sulfate sodium experimental murine colitis","volume":"69","author":"Cooper","year":"1993","journal-title":"Lab. Investig. J. Tech. Methods Pathol."},{"key":"ref_131","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1136\/pgmj.2004.030817","article-title":"Change of nitric oxide in experimental colitis and its inhibition by melatonin in vivo and in vitro","volume":"81","author":"Mei","year":"2005","journal-title":"Postgrad. Med. J."},{"key":"ref_132","unstructured":"Suvarna, K.S., Layton, C., and Bancroft, J.D. (2018). Bancroft\u2019s Theory and Practice of Histological Techniques E-Book, Elsevier Health Sciences."},{"key":"ref_133","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1136\/gut.47.3.404","article-title":"A reproducible grading scale for histological assessment of inflammation in ulcerative colitis","volume":"47","author":"Geboes","year":"2000","journal-title":"Gut"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/24\/12053\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,15]],"date-time":"2025-12-15T13:33:34Z","timestamp":1765805614000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/24\/12053"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,15]]},"references-count":133,"journal-issue":{"issue":"24","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["ijms262412053"],"URL":"https:\/\/doi.org\/10.3390\/ijms262412053","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12,15]]}}}