{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T03:24:49Z","timestamp":1782789889112,"version":"3.54.5"},"reference-count":70,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T00:00:00Z","timestamp":1765929600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"University-level research project of the Naval Medical University","award":["2024QN019"],"award-info":[{"award-number":["2024QN019"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Prolonged periods of sailing may contribute to the development of functional constipation, which can significantly impair an individual\u2019s work efficiency. Currently, the efficacy of Bifidobacteria in treating functional constipation is gaining recognition. However, since the therapeutic effects of Bifidobacteria are strain-specific, further research is required on strains isolated from pre-voyage fecal samples. This study examines the role of gut microbiota in post-stroke constipation, aiming to identify specific microbial biomarkers for the development of targeted therapeutic strategies. B. adolescentis was identified through metagenomic analysis and subsequently isolated for validation. In the experimental group (EG), C57BL\/6J mice received fecal suspension treatment following a 12-day navigation period, which was subsequently followed by a 12-day oral administration of B. adolescentis. After treatment, EG significantly improved fecal volume, intestinal motility, and goblet cells; reversed microbial ecological imbalance; reduced pathogens (E. coli and Klebsiella) by restoring arginine\/bile acid metabolism, decreasing Tauro-ursodeoxycholic acid (TUDCA) content, 5-Hydroxytryptamine 4 Receptor (5-HT4R)\/Slc8a1 signaling, and Ca2+ signaling pathway; and restoring beneficial species (B. adolescentis, Pseudomonas aeruginosa). This study provides new insights into probiotics in improving human intestinal health.<\/jats:p>","DOI":"10.3390\/ijms262412142","type":"journal-article","created":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T13:42:52Z","timestamp":1765978972000},"page":"12142","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Bifidobacterium adolescentis Strengthens Gut Barrier in Post-Voyage Functional Constipation"],"prefix":"10.3390","volume":"26","author":[{"given":"Huidie","family":"Zhao","sequence":"first","affiliation":[{"name":"Center of Translational Medicine Research, Naval Medical University, Shanghai 200433, China"},{"name":"College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongli","family":"Wang","sequence":"additional","affiliation":[{"name":"Center of Translational Medicine Research, Naval Medical University, Shanghai 200433, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyuan","family":"Zhao","sequence":"additional","affiliation":[{"name":"Center of Translational Medicine Research, Naval Medical University, Shanghai 200433, China"},{"name":"College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yishan","family":"Song","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong","family":"Liang","sequence":"additional","affiliation":[{"name":"Center of Translational Medicine Research, Naval Medical University, Shanghai 200433, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuhao","family":"Ma","sequence":"additional","affiliation":[{"name":"Center of Translational Medicine Research, Naval Medical University, Shanghai 200433, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheng","family":"Xu","sequence":"additional","affiliation":[{"name":"Center of Translational Medicine Research, Naval Medical University, Shanghai 200433, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,12,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e044633","DOI":"10.1136\/bmjopen-2020-044633","article-title":"Incidence of occupational injuries and diseases among seafarers: A descriptive epidemiological study based on contacts from onboard ships to the Italian Telemedical Maritime Assistance Service in Rome, Italy","volume":"11","author":"Sagaro","year":"2021","journal-title":"BMJ Open"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"761","DOI":"10.7326\/M15-3118","article-title":"Acupuncture for chronic severe functional constipation: A randomized trial","volume":"165","author":"Liu","year":"2016","journal-title":"Ann. Intern. Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1111\/j.1572-0241.2004.04043.x","article-title":"Psychosocial factors are linked to functional gastrointestinal disorders: A population based nested case-control study","volume":"99","author":"Locke","year":"2004","journal-title":"Am. J. Gastroenterol."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Brooks, S.K., and Greenberg, N. (2022). Mental health and psychological wellbeing of maritime personnel: A systematic review. BMC Psychol., 10.","DOI":"10.1186\/s40359-022-00850-4"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"e14207","DOI":"10.1111\/jsr.14207","article-title":"Sleep health among US Navy afloat versus ashore personnel in the Millennium Cohort Study","volume":"33","author":"Jacobson","year":"2024","journal-title":"J. Sleep Res."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"e231","DOI":"10.3346\/jkms.2022.37.e231","article-title":"Association of Depression with Susceptibility and Adaptation to Seasickness in the Military Seafarers","volume":"37","author":"Park","year":"2022","journal-title":"J. Korean Med. Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"106","DOI":"10.5603\/IMH.2015.0024","article-title":"Seafarer fatigue: A review of risk factors, consequences for seafarers\u2019 health and safety and options for mitigation","volume":"66","author":"Jepsen","year":"2015","journal-title":"Int. Marit. Health"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1038\/s41579-024-01068-4","article-title":"The interplay between diet and the gut microbiome: Implications for health and disease","volume":"22","author":"Ross","year":"2024","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"e0189922","DOI":"10.1128\/spectrum.01899-22","article-title":"Alterations in the Gut Microbiota and Metabolomics of Seafarers after a Six-Month Sea Voyage","volume":"10","author":"Jiang","year":"2022","journal-title":"Microbiol. Spectr."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"930","DOI":"10.1080\/19490976.2020.1722054","article-title":"Probiotics maintain the intestinal microbiome homeostasis of the sailors during a long sea voyage","volume":"11","author":"Zhang","year":"2020","journal-title":"Gut Microbes"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1594","DOI":"10.1111\/imj.15520","article-title":"The gut microbiota and gut disease","volume":"51","author":"Fehily","year":"2021","journal-title":"Intern. Med. J."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Markowiak-Kope\u0107, P., and \u015ali\u017cewska, K. (2020). The Effect of Probiotics on the Production of Short-Chain Fatty Acids by Human Intestinal Microbiome. Nutrients, 12.","DOI":"10.3390\/nu12041107"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1907271","DOI":"10.1080\/19490976.2021.1907271","article-title":"Completion of the gut microbial epi-bile acid pathway","volume":"13","author":"Doden","year":"2021","journal-title":"Gut Microbes"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1317","DOI":"10.1007\/s42770-024-01276-3","article-title":"Adjunctive efficacy of Bifidobacterium animalis subsp. lactis XLTG11 for functional constipation in children","volume":"55","author":"Chen","year":"2024","journal-title":"Braz. J. Microbiol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"e074557","DOI":"10.1136\/bmjopen-2023-074557","article-title":"Efficacy in bowel movement and change of gut microbiota on adult functional constipation patients treated with probiotics-containing products: A systematic review and meta-analysis","volume":"14","author":"Ding","year":"2024","journal-title":"BMJ Open"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1989","DOI":"10.1016\/j.chom.2023.10.011","article-title":"A key genetic factor governing arabinan utilization in the gut microbiome alleviates constipation","volume":"31","author":"Zhang","year":"2023","journal-title":"Cell Host Microbe"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"5037","DOI":"10.1039\/D1FO04151G","article-title":"Bifidobacterium longum relieves constipation by regulating the intestinal barrier of mice","volume":"13","author":"Wang","year":"2022","journal-title":"Food Funct."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"e70124","DOI":"10.1111\/cpr.70124","article-title":"The Impact of Maternal Gut Dysbiosis on Embryo\/Fetus Development","volume":"58","author":"Fan","year":"2025","journal-title":"Cell Prolif."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"712","DOI":"10.1093\/jpp\/rgad017","article-title":"APETx2 regulates intestinal motility and visceral sensitivity in post-infectious irritable bowel syndrome mice through 5-HT signalling pathway","volume":"75","author":"Yuan","year":"2023","journal-title":"J. Pharm. Pharmacol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"e14026","DOI":"10.1111\/nmo.14026","article-title":"Prokinetic actions of luminally acting 5-HT4 receptor agonists","volume":"33","author":"Konen","year":"2021","journal-title":"Neurogastroenterol. Motil."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1038\/s41422-022-00645-7","article-title":"Gut microbiota drives macrophage-dependent self-renewal of intestinal stem cells via niche enteric serotonergic neurons","volume":"32","author":"Zhu","year":"2022","journal-title":"Cell Res."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"2300615","DOI":"10.1002\/mnfr.202300615","article-title":"Torreya grandis Kernel Oil Alleviates Loperamide-Induced Slow Transit Constipation via Up-Regulating the Colonic Expressions of Occludin\/Claudin-1\/ZO-1 and 5-HT3R\/5-HT4R in BALB\/c Mice","volume":"68","author":"Wang","year":"2024","journal-title":"Mol. Nutr. Food Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"6058","DOI":"10.1039\/D1FO00559F","article-title":"Different Bifidobacterium bifidum strains change the intestinal flora composition of mice via different mechanisms to alleviate loperamide-induced constipation","volume":"12","author":"Chai","year":"2021","journal-title":"Food Funct."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"318","DOI":"10.3390\/ijms18020318","article-title":"Bifidobacterium adolescentis Exerts Strain-Specific Effects on Constipation Induced by Loperamide in BALB\/c Mice","volume":"18","author":"Wang","year":"2017","journal-title":"Int. J. Mol. Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1539","DOI":"10.1007\/s00109-021-02125-8","article-title":"AMPK: A key regulator of energy stress and calcium-induced autophagy","volume":"99","author":"Saikia","year":"2021","journal-title":"J. Mol. Med."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1038\/s41579-025-01183-w","article-title":"Gut microbiota-derived short-chain fatty acids and their role in human health and disease","volume":"23","author":"Mukhopadhya","year":"2025","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"e210","DOI":"10.1016\/S2665-9913(20)30004-7","article-title":"Safety and efficacy of metformin in systemic lupus erythematosus: A multicentre, randomised, double-blind, placebo-controlled trial","volume":"2","author":"Sun","year":"2020","journal-title":"Lancet Rheumatol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"121233","DOI":"10.1016\/j.carbpol.2023.121233","article-title":"Arabinoxylan source and xylanase specificity influence the production of oligosaccharides with prebiotic potential","volume":"320","author":"Rudjito","year":"2023","journal-title":"Carbohydr. Polym."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"117418","DOI":"10.1016\/j.carbpol.2020.117418","article-title":"Prevention of loperamide induced constipation in mice by KGM and the mechanisms of different gastrointestinal tract microbiota regulation","volume":"256","author":"Zhang","year":"2021","journal-title":"Carbohydr. Polym."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"991","DOI":"10.1038\/s43587-021-00129-0","article-title":"Bifidobacterium adolescentis regulates catalase activity and host metabolism and improves healthspan and lifespan in multiple species","volume":"1","author":"Chen","year":"2021","journal-title":"Nat. Aging"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"110482","DOI":"10.1016\/j.cbi.2023.110482","article-title":"Galangin as an inflammatory response modulator: An updated overview and therapeutic potential","volume":"378","author":"Thapa","year":"2023","journal-title":"Chem. Biol. Interact."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"McFarland, L.V., Evans, C.T., and Goldstein, E.J.C. (2018). Strain-Specificity and Disease-Specificity of Probiotic Efficacy: A Systematic Review and Meta-Analysis. Front. Med., 5.","DOI":"10.3389\/fmed.2018.00124"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1146\/annurev.nutr.22.110801.140547","article-title":"Regulation of enzymes of the urea cycle and arginine metabolism","volume":"22","author":"Morris","year":"2002","journal-title":"Annu. Rev. Nutr."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1016\/j.jhep.2024.09.032","article-title":"Bile acid metabolism and signalling in liver disease","volume":"82","author":"Fuchs","year":"2025","journal-title":"J. Hepatol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"eado8222","DOI":"10.1126\/sciadv.ado8222","article-title":"Artificial mucus layer formed in response to ROS for the oral treatment of inflammatory bowel disease","volume":"10","author":"Zhang","year":"2024","journal-title":"Sci. Adv."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Kageyama, S., Inoue, R., Hosomi, K., Park, J., Yumioka, H., Suka, T., Kurohashi, Y., Teramoto, K., Syauki, A.Y., and Doi, M. (2021). Effects of Malted Rice Amazake on Constipation Symptoms and Gut Microbiota in Children and Adults with Severe Motor and Intellectual Disabilities: A Pilot Study. Nutrients, 13.","DOI":"10.3390\/nu13124466"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"15087","DOI":"10.1021\/acs.jafc.3c03734","article-title":"Lychee Pulp-Derived Dietary Fiber-Bound Phenolic Complex Upregulates the SCFAs-GPRs-ENS Pathway and Aquaporins in Loperamide-Induced Constipated Mice by Reshaping Gut Microbiome","volume":"71","author":"Dong","year":"2023","journal-title":"J. Agric. Food Chem."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Wang, L., Chen, C., Cui, S., Lee, Y.-K., Wang, G., Zhao, J., Zhang, H., and Chen, W. (2019). Adhesive Bifidobacterium Induced Changes in Cecal Microbiome Alleviated Constipation in Mice. Front. Microbiol., 10.","DOI":"10.3389\/fmicb.2019.01721"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1186\/s12941-023-00630-x","article-title":"High prevalence of Mucosa-Associated extended-spectrum \u03b2-Lactamase-producing Escherichia coli and Klebsiella pneumoniae among Iranain patients with inflammatory bowel disease (IBD)","volume":"22","author":"Kharaghani","year":"2023","journal-title":"Ann. Clin. Microbiol. Antimicrob."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1969","DOI":"10.1038\/s41467-022-29552-6","article-title":"Microglial NF-\u03baB drives tau spreading and toxicity in a mouse model of tauopathy","volume":"13","author":"Wang","year":"2022","journal-title":"Nat. Commun."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1799","DOI":"10.1038\/s41386-020-0667-2","article-title":"Adverse effects of cannabidiol: A systematic review and meta-analysis of randomized clinical trials","volume":"45","author":"Chesney","year":"2020","journal-title":"Neuropsychopharmacology"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"e0024024","DOI":"10.1128\/cmr.00240-24","article-title":"Recent advances in therapeutic probiotics: Insights from human trials","volume":"38","author":"Cho","year":"2025","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"692","DOI":"10.1159\/000501959","article-title":"Hesperidin Improves Colonic Motility in Loeramide-Induced Constipation Rat Model via 5-Hydroxytryptamine 4R\/cAMP Signaling Pathway","volume":"101","author":"Wu","year":"2019","journal-title":"Digestion"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1080\/13880209.2022.2157841","article-title":"Therapeutic effects of Bombax ceiba flower aqueous extracts against loperamide-induced constipation in mice","volume":"61","author":"Wang","year":"2023","journal-title":"Pharm. Biol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"5882","DOI":"10.1007\/s12035-024-03958-3","article-title":"Total Flavonoids of Aurantii Fructus Immaturus Regulate miR-5100 to Improve Constipation by Targeting Fzd2 to Alleviate Calcium Balance and Autophagy in Interstitial Cells of Cajal","volume":"61","author":"Wen","year":"2024","journal-title":"Mol. Neurobiol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"117787","DOI":"10.1016\/j.biopha.2024.117787","article-title":"The inhibition of SLC8A1 promotes Ca2+-dependent cell death in Gastric Cancer","volume":"182","author":"Laurino","year":"2025","journal-title":"Biomed. Pharmacother."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Yang, Z., Wu, Y., Liu, X., Zhang, M., Peng, J., and Wei, H. (2022). S. boulardii Early Intervention Maintains Gut Microbiome Structure and Promotes Gut Mucosal Barrier Function in Early-Weaned Rats. Nutrients, 14.","DOI":"10.3390\/nu14173485"},{"key":"ref_48","first-page":"708","article-title":"Randomised placebo-controlled double blind multicentric trial on efficacy and safety of Lactobacillus acidophilus LA-5 and Bifidobacterium BB-12 for prevention of antibiotic-associated diarrhoea","volume":"61","author":"Chatterjee","year":"2013","journal-title":"J. Assoc. Physicians India"},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Li, B., Wang, H., Wang, M., Liang, H., Hu, T., Yang, J., Li, S., You, X., Xia, B., and Yuan, Y. (2025). Genome analysis of Bifidobacterium adolescentis and investigation of its effects on inflammation and intestinal barrier function. Front. Microbiol., 15.","DOI":"10.3389\/fmicb.2024.1496280"},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Liu, L., Deng, L., Wei, W., Li, C., Lu, Y., Bai, J., Li, L., Zhang, H., Jin, N., and Li, C. (2023). Lactiplantibacillus plantarum LPJZ-658 Improves Non-Alcoholic Steatohepatitis by Modulating Bile Acid Metabolism and Gut Microbiota in Mice. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms241813997"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"111479","DOI":"10.1016\/j.biopha.2021.111479","article-title":"Rhubarb extract relieves constipation by stimulating mucus production in the colon and altering the intestinal flora","volume":"138","author":"Gao","year":"2021","journal-title":"Biomed. Pharmacother."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"490","DOI":"10.1186\/s12967-021-03152-2","article-title":"Effect of fecal microbiota transplantation in patients with slow transit constipation and the relative mechanisms based on the protein digestion and absorption pathway","volume":"19","author":"Xie","year":"2021","journal-title":"J. Transl. Med."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1293","DOI":"10.1093\/ecco-jcc\/jjac020","article-title":"Role of Nutrient-sensing Receptor GPRC6A in Regulating Colonic Group 3 Innate Lymphoid Cells and Inflamed Mucosal Healing","volume":"16","author":"Hou","year":"2022","journal-title":"J. Crohns Colitis"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"106029","DOI":"10.1016\/j.intimp.2019.106029","article-title":"Arginine accelerates intestinal health through cytokines and intestinal microbiota","volume":"81","author":"Wu","year":"2020","journal-title":"Int. Immunopharmacol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"883","DOI":"10.1111\/jgh.15800","article-title":"Impact of elobixibat on serum and fecal bile acid levels and constipation symptoms in patients with chronic constipation","volume":"37","author":"Nakajima","year":"2022","journal-title":"J. Gastroenterol. Hepatol."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Chittimalli, K., Jahan, J., Sakamuri, A., and Jarajapu, Y.P. (2022). Angiotensin-(1-7) Restores Gut Barrier Integrity and Ameliorates Systemic Inflammation in Agin. FASEB J., 36.","DOI":"10.1096\/fasebj.2022.36.S1.R3069"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1765","DOI":"10.1016\/j.cmet.2022.09.024","article-title":"The gut peptide Reg3g links the small intestine microbiome to the regulation of energy balance, glucose levels, and gut function","volume":"34","author":"Shin","year":"2022","journal-title":"Cell Metab."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"734","DOI":"10.1631\/jzus.B2200439","article-title":"Lactobacillus gasseri LA39 promotes hepatic primary bile acid biosynthesis and intestinal secondary bile acid biotransformation","volume":"24","author":"Hu","year":"2023","journal-title":"J. Zhejiang Univ.-Sci. B"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1080\/19490976.2018.1549420","article-title":"In vitro and in vivo characterization of Clostridium scindens bile acid transformations","volume":"10","author":"Marion","year":"2019","journal-title":"Gut Microbes"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"e27472","DOI":"10.1016\/j.heliyon.2024.e27472","article-title":"Untargeted metabolomics uncovers metabolic dysregulation and tissue sensitivity in ACE2 knockout mice","volume":"10","author":"Zhao","year":"2024","journal-title":"Heliyon"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1038\/labinvest.2017.6","article-title":"Tauroursodeoxycholic acid protects bile acid homeostasis under inflammatory conditions and dampens Crohn\u2019s disease-like ileitis","volume":"97","author":"Borsboom","year":"2017","journal-title":"Lab. Investig."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1186\/s40104-022-00713-3","article-title":"Tauroursodeoxycholic acid (TUDCA) improves intestinal barrier function associated with TGR5-MLCK pathway and the alteration of serum metabolites and gut bacteria in weaned piglets","volume":"13","author":"Song","year":"2022","journal-title":"J. Anim. Sci. Biotechnol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1186\/s44149-024-00139-6","article-title":"Taurodeoxycholic acid alleviates diquat-induced intestinal barrier function injury in mice through the upregulation of Nrf2-mediated signaling pathway","volume":"4","author":"Liu","year":"2024","journal-title":"Anim. Dis."},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Ding, M., Yang, B., Khine, W.W.T., Lee, Y.-K., Rahayu, E.S., Ross, R.P., Stanton, C., Zhao, J., Zhang, H., and Chen, W. (2021). The Species-Level Composition of the Fecal Bifidobacterium and Lactobacillus Genera in Indonesian Children Differs from That of Their Mothers. Microorganisms, 9.","DOI":"10.3390\/microorganisms9091995"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.1007\/s00253-020-11084-y","article-title":"Metagenomic identification, purification and characterisation of the Bifidobacterium adolescentis BgaC \u03b2-galactosidase","volume":"105","author":"Mulualem","year":"2021","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"1595","DOI":"10.1039\/C9FO00014C","article-title":"A ropy exopolysaccharide producing strain Bifidobacterium longum subsp. longum YS108R alleviates DSS-induced colitis by maintenance of the mucosal barrier and gut microbiota modulation","volume":"10","author":"Yan","year":"2019","journal-title":"Food Funct."},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Genton, L., Lazarevic, V., Stojanovic, O., Spiljar, M., Djaafar, S., Koessler, T., Dutoit, V., Ga\u00efa, N., Mareschal, J., and Macpherson, A.J. (2021). Metataxonomic and Metabolic Impact of Fecal Microbiota Transplantation from Patients with Pancreatic Cancer into Germ-Free Mice: A Pilot Study. Front. Cell. Infect. Microbiol., 11.","DOI":"10.3389\/fcimb.2021.752889"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"10322","DOI":"10.1038\/s41598-017-10835-8","article-title":"Dysbiosis contributes to chronic constipation development via regulation of serotonin transporter in the intestine","volume":"7","author":"Cao","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"1741","DOI":"10.1053\/j.gastro.2018.08.022","article-title":"FXR-Dependent Modulation of the Human Small Intestinal Microbiome by the Bile Acid Derivative Obeticholic Acid","volume":"155","author":"Friedman","year":"2018","journal-title":"Gastroenterology"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"e00561-20","DOI":"10.1128\/MRA.00561-20","article-title":"Draft Genome Sequence of Bifidobacterium pseudocatenulatum Bif4, Isolated from Healthy Infant Feces","volume":"9","author":"Chander","year":"2020","journal-title":"Microbiol. Resour. Announc."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/24\/12142\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,19]],"date-time":"2025-12-19T05:20:49Z","timestamp":1766121649000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/24\/12142"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,17]]},"references-count":70,"journal-issue":{"issue":"24","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["ijms262412142"],"URL":"https:\/\/doi.org\/10.3390\/ijms262412142","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12,17]]}}}