{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:52:45Z","timestamp":1792788765493,"version":"4.5.0"},"reference-count":85,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2025,12,18]],"date-time":"2025-12-18T00:00:00Z","timestamp":1766016000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006701","name":"Umm al-Qura University","doi-asserted-by":"publisher","award":["25UQU4310007GSSR05"],"award-info":[{"award-number":["25UQU4310007GSSR05"]}],"id":[{"id":"10.13039\/501100006701","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Acute kidney injury (AKI) is defined as a quick and often reversible decline in renal performance, as shown by elevated creatinine or reduced urine volume. AKI is a common illness, particularly among hospitalized cases, and can be observed in up to 7% of hospital admissions and 30% of ICU admissions. This study was designed to explore the nephroprotective potential of eco-synthesized quercetin\u2013selenium nanoparticles (QUR-SeNPs) against experimentally glycerol-induced rhabdomyolysis leading to AKI. Forty healthy adult male albino rats were employed in the experiment. Animals were randomly distributed equally into five groups: Control: orally administered with normal saline solution. GLY: orally administered with normal saline (0.9% NaCl) for 15 consecutive days, at day 14, animals of this group received a single dose of intramuscular (im.) injection of 50% glycerol (GLY) (10 mg\/kg\/day). GLY and quercetin (GLY&amp;QUR): orally administered with quercetin daily for 15 days (50 mg\/kg\/day), at day 14, animals of this group received a single dose of im. injection of 50% glycerol (10 mg\/kg\/day). GLY&amp;Na2SeO3: orally administered with sodium selenite daily for 15 days (0.5 mg\/kg\/day), at day 14, animals of this group received a single dose of im. injection of 50% glycerol (10 mg\/kg\/day). GLY&amp;QUR-SeNPs: orally administered with selenium nanoparticles synthesized using quercetin daily for 15 days (0.5 mg\/kg\/day), at day 14, animals of this group received a single dose of im. injection of 50% glycerol (10 mg\/kg\/day). Oxidative stress, inflammatory, and apoptotic markers, in addition to histopathological, gene expression, and immunohistochemical analysis, were assessed for all groups. The results demonstrated that QUR-SeNPs effectively ameliorated renal functional, biochemical, and molecular disturbances through their synergistic antioxidant, anti-inflammatory, and anti-apoptotic potential, surpassing the effects of either quercetin or selenium alone. Biosynthesized selenium nanoparticles using QUR-SeNPs demonstrated remarkable nephroprotective activity by normalizing renal biomarkers, restoring antioxidant capacity, inhibiting inflammatory cytokines, and preventing apoptotic damage. The nanoparticle formulation exhibited superior efficacy to either QUR or Se alone, highlighting the synergistic interplay between selenium and quercetin through enhanced bioavailability, redox stability, and molecular targeting.<\/jats:p>","DOI":"10.3390\/ijms262412187","type":"journal-article","created":{"date-parts":[[2025,12,18]],"date-time":"2025-12-18T16:42:05Z","timestamp":1766076125000},"page":"12187","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Nephroprotective Effects of Quercetin\u2013Selenium Nanoparticles Against Glycerol-Induced AKI"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2118-0499","authenticated-orcid":false,"given":"Ahmed M.","family":"Ashour","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Toxicology, College of Pharmacy, Umm Al-Qura University, P.O. Box 13578, Makkah 21955, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali","family":"Khames","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, Faculty of Pharmacy, Sohag University, Sohag 82511, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-1140-8338","authenticated-orcid":false,"given":"Khaled M.","family":"Alam-ElDein","sequence":"additional","affiliation":[{"name":"Molecular Biology and Biotechnology Department, School of Biotechnology, Badr University in Cairo, Cairo 11829, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2568-6059","authenticated-orcid":false,"given":"Ahmed Hassan Ibrahim","family":"Faraag","sequence":"additional","affiliation":[{"name":"Molecular Biology and Biotechnology Department, School of Biotechnology, Badr University in Cairo, Cairo 11829, Egypt"},{"name":"Botany and Microbiology Department, Faculty of Science, Helwan University, Cairo 11795, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nievin Ahmed","family":"Mahran","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Biotechnology, Sinai University, Kantara Branch, Ismailia 41636, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Badriyah","family":"Aljazzaf","sequence":"additional","affiliation":[{"name":"Department of Food Sciences and Nutrition, College of Health Sciences, The Public Authority for Applied Education and Training, Kuwait City 13092, Kuwait"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1361-2933","authenticated-orcid":false,"given":"Rabia","family":"Alghazeer","sequence":"additional","affiliation":[{"name":"Chemistry Department, Faculty of Science, University of Tripoli, Tripoli 50676, Libya"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatma","family":"Akmal","sequence":"additional","affiliation":[{"name":"Human Anatomy and Embryology Department, Faculty of Medicine, Zagazig University, Zagazig 44519, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marwa Ahmed","family":"Mahmoud","sequence":"additional","affiliation":[{"name":"Department of Medical Physiology, Faculty of Medicine, Sohag University, Sohag 82511, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1976-9480","authenticated-orcid":false,"given":"Mohamed H. A.","family":"Gadelmawla","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, Faculty of Biotechnology, Sinai University, Kantara Branch, Ismailia 41636, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,12,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"103938","DOI":"10.1016\/j.drudis.2024.103938","article-title":"Generation of a Drug-Induced Renal Injury List to Facilitate the Development of New Approach Methodologies for Nephrotoxicity","volume":"29","author":"Connor","year":"2024","journal-title":"Drug Discov. Today"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Hosohata, K. (2016). Role of Oxidative Stress in Drug-Induced Kidney Injury. Int. J. Mol. Sci., 17.","DOI":"10.3390\/ijms17111826"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Leng, X.-Y., Liu, C.-N., Wang, S.-C., Peng, H.-D., Wang, D.-G., and Pan, H.-F. (2022). 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