{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,25]],"date-time":"2025-12-25T05:21:25Z","timestamp":1766640085827,"version":"3.48.0"},"reference-count":75,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2025,12,21]],"date-time":"2025-12-21T00:00:00Z","timestamp":1766275200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003141","name":"Consejo Nacional de Ciencia y Tecnolog\u00eda","doi-asserted-by":"crossref","award":["300727"],"award-info":[{"award-number":["300727"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The Tepetitl\u00e1n reservoir is affected by untreated domestic wastewater, agricultural runoff, and livestock-related discharges, posing risks to aquatic ecosystems. This study assessed its water quality and evaluated toxic effects on adult zebrafish (Danio rerio). Water samples from six sites (A\u2013F) were analyzed for physicochemical parameters and the presence of metals and pharmaceuticals, quantified at concentrations in the \u00b5g L\u22121 range. While most physicochemical parameters complied with Mexican regulations, true color exceeded established limits, and the presence of contaminants indicated environmental deterioration. Zebrafish were exposed to water from each site for 12, 24, 48, 72, and 96 h. Oxidative stress (OS) biomarkers were measured in the brain, gill, gut, and liver at all time points, and gene expression of antioxidant, detoxification, and apoptosis-related genes was assessed at 96 h in these organs. Significant OS was detected across sites, with increased lipid peroxidation, protein carbonylation, and hydroperoxide levels. Although antioxidant enzymes were activated, their response did not fully counteract oxidative damage. Gene expression analysis revealed upregulation of stress- and apoptosis-related genes. These findings demonstrate that the Tepetitl\u00e1n reservoir contains pollutant mixtures capable of inducing oxidative and molecular stress responses in zebrafish, underlining potential ecological risks and the need for mitigation efforts.<\/jats:p>","DOI":"10.3390\/ijms27010075","type":"journal-article","created":{"date-parts":[[2025,12,24]],"date-time":"2025-12-24T14:27:51Z","timestamp":1766586471000},"page":"75","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Multiorgan Oxidative Damage and Gene Expression Modulation in Danio rerio Induced by Pollutants from the Tepetitl\u00e1n Reservoir, Mexico"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2660-2782","authenticated-orcid":false,"given":"Nely","family":"San Juan-Reyes","sequence":"first","affiliation":[{"name":"Laboratorio de Toxicolog\u00eda Ambiental, Facultad de Qu\u00edmica, Universidad Aut\u00f3noma del Estado de M\u00e9xico, Paseo Col\u00f3n intersecci\u00f3n Paseo Tollocan, Colonia Residencial Col\u00f3n, Toluca CP 50120, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7248-3449","authenticated-orcid":false,"given":"Leobardo Manuel","family":"G\u00f3mez-Oliv\u00e1n","sequence":"additional","affiliation":[{"name":"Laboratorio de Toxicolog\u00eda Ambiental, Facultad de Qu\u00edmica, Universidad Aut\u00f3noma del Estado de M\u00e9xico, Paseo Col\u00f3n intersecci\u00f3n Paseo Tollocan, Colonia Residencial Col\u00f3n, Toluca CP 50120, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 Manuel","family":"Orozco-Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Laboratorio de Toxicolog\u00eda Ambiental, Facultad de Qu\u00edmica, Universidad Aut\u00f3noma del Estado de M\u00e9xico, Paseo Col\u00f3n intersecci\u00f3n Paseo Tollocan, Colonia Residencial Col\u00f3n, Toluca CP 50120, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-0758-3016","authenticated-orcid":false,"given":"Eduardo Javier","family":"Quiroz-Fabela","sequence":"additional","affiliation":[{"name":"Laboratorio de Toxicolog\u00eda Ambiental, Facultad de Qu\u00edmica, Universidad Aut\u00f3noma del Estado de M\u00e9xico, Paseo Col\u00f3n intersecci\u00f3n Paseo Tollocan, Colonia Residencial Col\u00f3n, Toluca CP 50120, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francisco","family":"Escobar-Huerfano","sequence":"additional","affiliation":[{"name":"Laboratorio de Toxicolog\u00eda Ambiental, Facultad de Qu\u00edmica, Universidad Aut\u00f3noma del Estado de M\u00e9xico, Paseo Col\u00f3n intersecci\u00f3n Paseo Tollocan, Colonia Residencial Col\u00f3n, Toluca CP 50120, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karina Elisa","family":"Rosales-P\u00e9rez","sequence":"additional","affiliation":[{"name":"Laboratorio de Toxicolog\u00eda Ambiental, Facultad de Qu\u00edmica, Universidad Aut\u00f3noma del Estado de M\u00e9xico, Paseo Col\u00f3n intersecci\u00f3n Paseo Tollocan, Colonia Residencial Col\u00f3n, Toluca CP 50120, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3119-463X","authenticated-orcid":false,"given":"Gustavo Axel","family":"Elizalde-Vel\u00e1zquez","sequence":"additional","affiliation":[{"name":"Laboratorio de Toxicolog\u00eda Ambiental, Facultad de Qu\u00edmica, Universidad Aut\u00f3noma del Estado de M\u00e9xico, Paseo Col\u00f3n intersecci\u00f3n Paseo Tollocan, Colonia Residencial Col\u00f3n, Toluca CP 50120, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sindy","family":"San Juan-Reyes","sequence":"additional","affiliation":[{"name":"Departamento de Neonatolog\u00eda, Hospital de Ginecolog\u00eda y Obstetricia No. 221, Instituto Mexicano del Seguro Social (IMSS), Avenida Josefa Ortiz De Dom\u00ednguez 304, Toluca CP 50150, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Selene Elizabeth","family":"Herrera-V\u00e1zquez","sequence":"additional","affiliation":[{"name":"Laboratorio de Toxicolog\u00eda Ambiental, Facultad de Qu\u00edmica, Universidad Aut\u00f3noma del Estado de M\u00e9xico, Paseo Col\u00f3n intersecci\u00f3n Paseo Tollocan, Colonia Residencial Col\u00f3n, Toluca CP 50120, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,12,21]]},"reference":[{"key":"ref_1","first-page":"91","article-title":"Conflicto Por El Agua En El Sistema de Riego Tepetitl\u00e1n Despu\u00e9s de La Trasferencia","volume":"24","year":"2012","journal-title":"Reg. 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