{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T04:09:24Z","timestamp":1791950964302,"version":"4.3.4"},"reference-count":73,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2025,12,25]],"date-time":"2025-12-25T00:00:00Z","timestamp":1766620800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004704","name":"National Research Council of Thailand","doi-asserted-by":"publisher","award":["N41A650402"],"award-info":[{"award-number":["N41A650402"]}],"id":[{"id":"10.13039\/501100004704","id-type":"DOI","asserted-by":"publisher"}]},{"name":"TAI.C.M.S. Standard industrial company limited"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>With an aging population and the increase in life expectancy, colorectal cancer is becoming increasingly common. Currently, the mainstay of treatment is chemotherapy, which can cause a variety of side effects. However, herbalists in many cultures have long used herbs to promote health and treat various diseases, including colon cancer. This study investigated the chemical components of shell extracts from Plukenetia volubilis (sacha inchi) and their effects on colon cancer cells. First, cytotoxicity against both normal and cancer cells was assessed, followed by cell migration and invasion. Finally, transcriptomic and proteomic analyses were performed to investigate molecular mechanisms. Phytochemical analysis showed a total phenolic content of 134.61 \u00b1 0.27 mgGAE\/g extract and a total flavonoid content of 5.75 \u00b1 0.01 mgQE\/g extract; moreover, lidocaine (10.57%) and linolenic acid (10.39%) were identified as the most abundant compounds. In vitro, the extract inhibited cell migration, invasion, and colony formation and was associated with potential modulations of the Hippo pathways and epithelial\u2013mesenchymal transition. Therefore, it can be concluded that these extracts are effective in inhibiting the progression of colon cancer cells, and thus, they can prospectively be developed as a dietary supplement or therapeutic agent for the treatment of colon cancer in the future.<\/jats:p>","DOI":"10.3390\/ijms27010234","type":"journal-article","created":{"date-parts":[[2025,12,26]],"date-time":"2025-12-26T00:50:21Z","timestamp":1766710221000},"page":"234","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Anticancer Effects of Sacha Inchi (Plukenetia volubilis) Shell Extract on Colon Cancer Cells: Integrated GC-MS, LC-MS, Transcriptomic, and Proteomic Analyses"],"prefix":"10.3390","volume":"27","author":[{"given":"Supawadee","family":"Osotprasit","sequence":"first","affiliation":[{"name":"Faculty of Allied Health Sciences, Burapha University, Chonburi 20131, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9129-2082","authenticated-orcid":false,"given":"Saowaros","family":"Suwansa-Ard","sequence":"additional","affiliation":[{"name":"Centre for Bioinnovation, University of the Sunshine Coast, Maroochydore, QLD 4558, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1454-2076","authenticated-orcid":false,"given":"Scott F.","family":"Cummins","sequence":"additional","affiliation":[{"name":"Centre for Bioinnovation, University of the Sunshine Coast, Maroochydore, QLD 4558, Australia"},{"name":"School of Science, Technology and Engineering, University of the Sunshine Coast, Sippy Downs, QLD 4558, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4876-7767","authenticated-orcid":false,"given":"Tianfang","family":"Wang","sequence":"additional","affiliation":[{"name":"Centre for Bioinnovation, University of the Sunshine Coast, Maroochydore, QLD 4558, Australia"},{"name":"School of Science, Technology and Engineering, University of the Sunshine Coast, Sippy Downs, QLD 4558, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-3656-2673","authenticated-orcid":false,"given":"Stuart J.","family":"Smith","sequence":"additional","affiliation":[{"name":"Centre for Bioinnovation, University of the Sunshine Coast, Maroochydore, QLD 4558, Australia"},{"name":"School of Science, Technology and Engineering, University of the Sunshine Coast, Sippy Downs, QLD 4558, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tepparit","family":"Samrit","sequence":"additional","affiliation":[{"name":"Faculty of Allied Health Sciences, Burapha University, Chonburi 20131, Thailand"},{"name":"Food Bioactive Compounds Research Unit, Faculty of Allied Health Sciences, Burapha University, Chonburi 20131, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Athit","family":"Chaiwichien","sequence":"additional","affiliation":[{"name":"Faculty of Allied Health Sciences, Burapha University, Chonburi 20131, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Narin","family":"Changklungmoa","sequence":"additional","affiliation":[{"name":"Faculty of Allied Health Sciences, Burapha University, Chonburi 20131, Thailand"},{"name":"Food Bioactive Compounds Research Unit, Faculty of Allied Health Sciences, Burapha University, Chonburi 20131, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4229-0803","authenticated-orcid":false,"given":"Pornanan","family":"Kueakhai","sequence":"additional","affiliation":[{"name":"Faculty of Allied Health Sciences, Burapha University, Chonburi 20131, Thailand"},{"name":"Food Bioactive Compounds Research Unit, Faculty of Allied Health Sciences, Burapha University, Chonburi 20131, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,12,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"27034","DOI":"10.1038\/s41598-024-76947-0","article-title":"Repurposing of sericin combined with dactolisib or vitamin D to combat non-small lung cancer cells through computational and biological investigations","volume":"14","author":"Helmy","year":"2024","journal-title":"Sci. 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