{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T01:22:27Z","timestamp":1792804947256,"version":"4.5.0"},"reference-count":24,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2025,12,30]],"date-time":"2025-12-30T00:00:00Z","timestamp":1767052800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Temas Libres","award":["930\/17"],"award-info":[{"award-number":["930\/17"]}]},{"name":"FONDECYT","award":["1181699"],"award-info":[{"award-number":["1181699"]}],"id":[{"id":"https:\/\/ror.org\/016nafs32","id-type":"ROR","asserted-by":"crossref"}]},{"name":"FONDECYT","award":["1231596"],"award-info":[{"award-number":["1231596"]}],"id":[{"id":"https:\/\/ror.org\/016nafs32","id-type":"ROR","asserted-by":"crossref"}]},{"name":"OAIC"},{"name":"HCUCH","id":[{"id":"https:\/\/ror.org\/02xtpdq88","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Metabolic dysfunction-associated steatotic liver disease (MASLD) has become highly prevalent worldwide, and its pathogenesis and progression mechanisms remain incompletely understood. An increased activation of innate immune cells in the liver contributes to hepatic fibrogenesis via a chronic loop of inflammation and regeneration processes. Among them are mast cells (MCs), whose role in hepatic cirrhosis secondary to MASLD remains poorly studied. Our aim was to evaluate differences in MC density in cirrhotic liver tissue among patients with MASLD and other chronic liver disease etiologies. For this, a retrospective study of MC count was performed in cirrhotic liver explants obtained from MASLD, alcohol-related liver disease (ALD), and autoimmune hepatitis (AIH). We included a control group of subjects without liver damage. Tryptase-positive MCs were identified by indirect immunofluorescence and quantified as MC density per low-power field (MC\/LPF). Group differences were analyzed using the Kruskal\u2013Wallis test with Dunn\u2019s multiple comparisons, considering p &lt; 0.05 as statistically significant. A significantly higher MC density was observed in MASLD, ALD, and AIH patients compared with the control group. The group analysis showed that ALD patients exhibited higher MC density than AIH, with no observed difference between ALD and MASLD. MC density was correlated positively with tobacco smoking and alcohol use in the full analyzed group, suggesting them as risk factors of high MC liver infiltration. We conclude that MC density is augmented in MASLD-related cirrhosis, highlighting potential links between lifestyle factors and MC-mediated hepatic inflammation. Future studies should explore the mechanisms driving this association and evaluate whether targeting MCs could help mitigate fibrosis progression.<\/jats:p>","DOI":"10.3390\/ijms27010392","type":"journal-article","created":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T15:30:32Z","timestamp":1767195032000},"page":"392","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Increased Intrahepatic Mast Cell Density in Liver Cirrhosis Due to MASLD and Other Non-Infectious Chronic Liver Diseases"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9755-8593","authenticated-orcid":false,"given":"Nicol\u00e1s","family":"Ortiz-L\u00f3pez","sequence":"first","affiliation":[{"name":"Laboratory of Immunogastroenterology I, Section of Gastroenterology, Department of Medicine, Hospital Cl\u00ednico Universidad de Chile, Santiago 8380456, Chile"},{"name":"Graduate School, Faculty of Medicine, Universidad de Chile, Santiago 8380453, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3936-4111","authenticated-orcid":false,"given":"Araceli","family":"Pinto-Le\u00f3n","sequence":"additional","affiliation":[{"name":"Laboratory of Immunogastroenterology I, Section of Gastroenterology, Department of Medicine, Hospital Cl\u00ednico Universidad de Chile, Santiago 8380456, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javiera","family":"Favi","sequence":"additional","affiliation":[{"name":"Laboratory of Immunogastroenterology I, Section of Gastroenterology, Department of Medicine, Hospital Cl\u00ednico Universidad de Chile, Santiago 8380456, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dannette","family":"Gu\u00ed\u00f1ez Francois","sequence":"additional","affiliation":[{"name":"Section of Gastroenterology, Department of Medicine, Hospital Cl\u00ednico Universidad de Chile, Santiago 8380456, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Larissa","family":"Aleman","sequence":"additional","affiliation":[{"name":"Laboratory of Immunogastroenterology I, Section of Gastroenterology, Department of Medicine, Hospital Cl\u00ednico Universidad de Chile, Santiago 8380456, Chile"},{"name":"Graduate School, Faculty of Medicine, Universidad de Chile, Santiago 8380453, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Carre\u00f1o-Toro","sequence":"additional","affiliation":[{"name":"Department of Pathology, Hospital Cl\u00ednico Universidad de Chile, Santiago 8380456, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alejandra","family":"Zazueta","sequence":"additional","affiliation":[{"name":"Program of Microbiology and Mycology, Institute of Biomedical Sciences, Faculty of Medicine, Universidad de Chile, Santiago 8380453, Chile"},{"name":"Medical Technology, Faculty of Medicine Cl\u00ednica Alemana, Universidad del Desarrollo, Santiago 7630426, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabien","family":"Magne","sequence":"additional","affiliation":[{"name":"Program of Microbiology and Mycology, Institute of Biomedical Sciences, Faculty of Medicine, Universidad de Chile, Santiago 8380453, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaime","family":"Poniachik","sequence":"additional","affiliation":[{"name":"Section of Gastroenterology, Department of Medicine, Hospital Cl\u00ednico Universidad de Chile, Santiago 8380456, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroll J.","family":"Beltr\u00e1n","sequence":"additional","affiliation":[{"name":"Laboratory of Immunogastroenterology I, Section of Gastroenterology, Department of Medicine, Hospital Cl\u00ednico Universidad de Chile, Santiago 8380456, Chile"},{"name":"Laboratory of Immunogastroenterology II, Department of Clinical Biochemistry and Immunology, Faculty of Pharmacy, Universidad de Concepci\u00f3n, Concepci\u00f3n 4070386, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,12,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e34934","DOI":"10.1097\/MD.0000000000034934","article-title":"Prevalence of metabolic dysfunction-associated steatotic liver disease (MASLD) in a middle-aged population with overweight and normal liver enzymes, and diagnostic accuracy of noninvasive proxies","volume":"103","author":"Boone","year":"2024","journal-title":"Medicine"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Yanai, H., Adachi, H., Hakoshima, M., Iida, S., and Katsuyama, H. 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