{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,26]],"date-time":"2026-07-26T02:51:01Z","timestamp":1785034261405,"version":"3.55.0"},"reference-count":73,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2025,12,30]],"date-time":"2025-12-30T00:00:00Z","timestamp":1767052800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100011711","name":"Department of Agriculture of Guangdong Province","doi-asserted-by":"publisher","award":["2024-XPY-00-016"],"award-info":[{"award-number":["2024-XPY-00-016"]}],"id":[{"id":"10.13039\/501100011711","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Lion-head goose is a large-sized breed native to Guangdong Province, China, exhibits remarkable capacity for fatty liver production under overfeeding conditions and is highly valued by local farmers and consumers. However, the molecular mechanisms driving fatty liver development in this breed are still unknown. In this study, we evaluated liver weight differences between normally fed and overfed Lion-head geese and further examined sex-specific differences following overfeeding. Overfeeding significantly increased liver weight more than 340%, and males possess a stronger capacity for lipid deposition under the same feeding regimen compared with females. RNA-Seq analysis identified 1476 differentially expressed genes (DEGs) shared by both sexes, which were mainly enriched in lipid and energy metabolism, oxidative stress, and mitochondrial pathways. In addition, 627 male-specific and 420 female-specific DEGs revealed sex-dependent differences, with males showing stronger transcriptional regulation and females exhibiting enhanced antioxidant and detoxification responses. Weighted gene co-expression network analysis (WGCNA) revealed 320 co-hub genes enriched in lipid and energy metabolism in overfeeding-induced fatty liver, along with 9 co-hub genes related to sex differences. Alternative splicing (AS) analysis detected 131 differentially spliced genes (DSGs). Integration of both approaches identified 7 overlapping genes, HYCC2 (Hyccin PI4KA lipid kinase complex subunit 2), AGL (Amylo-Alpha-1,6-Glucosidase and 4-Alpha-Glucanotransferase), CCDC62 (Coiled-coil domain containing 62), IGSF5 (Immunoglobulin superfamily member 5), MGARP (Mitochondria-localized glutamic acid-rich protein), CD80 (Cluster of Differentiation 80), and FPGS (Folylpolyglutamate synthase), as potential key regulators. These findings provide new insights into transcriptional and post-transcriptional regulation of overfeeding-induced fatty liver in geese.<\/jats:p>","DOI":"10.3390\/ijms27010407","type":"journal-article","created":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T15:30:32Z","timestamp":1767195032000},"page":"407","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Weighted Gene Co-Expression Network Analysis and Alternative Splicing Analysis Reveal Key Genes Regulating Overfeeding-Induced Fatty Liver in Lion-Head Goose"],"prefix":"10.3390","volume":"27","author":[{"given":"Jing","family":"Fu","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yezhi","family":"Lan","sequence":"additional","affiliation":[{"name":"Sanya Institute, China Agricultural University, Sanya 572025, China"},{"name":"State Key Laboratory of Animal Biotech Breeding, National Engineering Laboratory for Animal Breeding, MOA Key Laboratory of Animal Genetics and Breeding, College of Animal Science and Technology, Frontiers Science Center for Molecular Design Breeding, Beijing Key Laboratory for Animal Genetic Improvement, China Agricultural University, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuwen","family":"Liang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-5605-5470","authenticated-orcid":false,"given":"Xiaoguang","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China"},{"name":"College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruize","family":"Tang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuchuan","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yabiao","family":"Luo","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Biotech Breeding, National Engineering Laboratory for Animal Breeding, MOA Key Laboratory of Animal Genetics and Breeding, College of Animal Science and Technology, Frontiers Science Center for Molecular Design Breeding, Beijing Key Laboratory for Animal Genetic Improvement, China Agricultural University, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunpeng","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,12,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1007\/BF02537194","article-title":"Plasma lipoproteins and liver lipids in two breeds of geese with different susceptibility to hepatic steatosis: Changes induced by development and force-feeding","volume":"26","author":"Hermier","year":"1991","journal-title":"Lipids"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Zhao, M., Xing, Y., Liu, L., Fan, X., Liu, L., Geng, T., and Gong, D. (2020). GC-TOF-MS-Based Metabolomics Analyses of Liver and Intestinal Contents in the Overfed vs. Normally-Fed Geese. Animals, 10.","DOI":"10.3390\/ani10122375"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"giad003","DOI":"10.1093\/gigascience\/giad003","article-title":"Chromosome-level genome assembly of goose provides insight into the adaptation and growth of local goose breeds","volume":"12","author":"Zhao","year":"2022","journal-title":"Gigascience"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Chen, J., Zhang, S., Chen, G., Deng, X., Zhang, D., Wen, H., Yin, Y., Lin, Z., Zhang, X., and Luo, W. (2022). Transcriptome Sequencing Reveals Pathways Related to Proliferation and Differentiation of Shitou Goose Myoblasts. Animals, 12.","DOI":"10.3390\/ani12212956"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"103280","DOI":"10.1016\/j.psj.2023.103280","article-title":"Transcriptome and lipidome integration unveils mechanisms of fatty liver formation in Shitou geese","volume":"103","author":"Hong","year":"2024","journal-title":"Poult. Sci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1457","DOI":"10.1002\/hep.30626","article-title":"Sex Differences in Nonalcoholic Fatty Liver Disease: State of the Art and Identification of Research Gaps","volume":"70","author":"Lonardo","year":"2019","journal-title":"Hepatology"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1126\/science.ade7614","article-title":"Sex matters in liver fat regulation","volume":"378","author":"Waxman","year":"2022","journal-title":"Science"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"101533","DOI":"10.1016\/j.animal.2025.101533","article-title":"Spontaneous steatosis stimulation in geese induces liver fattening but impacts sexual maturation and muscle growth in a sex-dependent manner","volume":"19","author":"Knudsen","year":"2025","journal-title":"Animal"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"105305","DOI":"10.1016\/j.psj.2025.105305","article-title":"RNA sequencing and genome-wide association analysis reveal key genes responsible for different feather colors in Youjiang goose","volume":"104","author":"Zhao","year":"2025","journal-title":"Poult. Sci."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Tang, J., Ouyang, H., Chen, X., Jiang, D., Tian, Y., Huang, Y., and Shen, X. (2023). Comparative Transcriptome Analyses of Leg Muscle during Early Growth between Geese (Anser cygnoides) Breeds Differing in Body Size Characteristics. Genes, 14.","DOI":"10.3390\/genes14051048"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1016\/j.molcel.2019.09.017","article-title":"Alternative Splicing Regulatory Networks: Functions, Mechanisms, and Evolution","volume":"76","author":"Ule","year":"2019","journal-title":"Mol. Cell"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1229","DOI":"10.1074\/jbc.R700033200","article-title":"Genome-wide analysis of alternative pre-mRNA splicing","volume":"283","author":"Hartmann","year":"2008","journal-title":"J. Biol. Chem."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"386","DOI":"10.1038\/nrm1645","article-title":"Understanding alternative splicing: Towards a cellular code","volume":"6","author":"Matlin","year":"2005","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"e2405157","DOI":"10.1002\/advs.202405157","article-title":"The Spatial-Temporal Alternative Splicing Profile Reveals the Functional Diversity of FXR1 Isoforms in Myogenesis","volume":"11","author":"Wang","year":"2024","journal-title":"Adv. Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1080\/00071668.2020.1812527","article-title":"Research progress into the physiological changes in metabolic pathways in waterfowl with hepatic steatosis","volume":"62","author":"Wei","year":"2021","journal-title":"Br. Poult. Sci."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"H\u00e9rault, F., Hou\u00e9e-Bigot, M., Ba\u00e9za, E., Bouchez, O., Esquerr\u00e9, D., Klopp, C., and Diot, C. (2019). RNA-seq analysis of hepatic gene expression of common Pekin, Muscovy, mule and hinny ducks fed ad libitum or overfed. BMC Genom., 20.","DOI":"10.1186\/s12864-018-5415-1"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1274","DOI":"10.1021\/acs.biochem.2c00624","article-title":"Effects of UBE3A on Cell and Liver Metabolism Through the Ubiquitination of PDHA1 and ACAT1","volume":"62","author":"Peng","year":"2023","journal-title":"Biochemistry"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"9245699","DOI":"10.1155\/jobe\/9245699","article-title":"Genomic Exploration of Nonalcoholic Fatty Liver Disease: Insights From Gene Expression and Variation in Morbidly Obese Individuals","volume":"2025","author":"Rafaa","year":"2025","journal-title":"J. Obes."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1407","DOI":"10.1002\/jcp.31041","article-title":"The role of GPLD1 in chronic diseases","volume":"238","author":"Cao","year":"2023","journal-title":"J. Cell. Physiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"4214","DOI":"10.1038\/s41467-024-48471-2","article-title":"Liver ACOX1 regulates levels of circulating lipids that promote metabolic health through adipose remodeling","volume":"15","author":"Lu","year":"2024","journal-title":"Nat. Commun."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"E5593","DOI":"10.1073\/pnas.1419161111","article-title":"rMATS: Robust and flexible detection of differential alternative splicing from replicate RNA\u2013Seq data","volume":"111","author":"Shen","year":"2014","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Shimizu, K., Nishimuta, S., Fukumura, Y., Michinaga, S., Egusa, Y., Hase, T., Terada, T., Sakurai, F., Mizuguchi, H., and Tomita, K. (2022). Liver-specific overexpression of lipoprotein lipase improves glucose metabolism in high-fat diet-fed mice. PLoS ONE, 17.","DOI":"10.1371\/journal.pone.0274297"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"7184","DOI":"10.1038\/s41467-024-51489-1","article-title":"The lipid-metabolism enzyme ECI2 reduces neutrophil extracellular traps formation for colorectal cancer suppression","volume":"15","author":"Chen","year":"2024","journal-title":"Nat. Commun."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Wang, Y., Li, X., Cao, Y., Xiao, C., Liu, Y., Jin, H., and Cao, Y. (2021). Effect of the ACAA1 Gene on Preadipocyte Differentiation in Sheep. Front. Genet., 12.","DOI":"10.3389\/fgene.2021.649140"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"R453","DOI":"10.1152\/ajpregu.00198.2019","article-title":"Kinetic study of the expression of genes related to hepatic steatosis, glucose and lipid metabolism, and cellular stress during overfeeding in mule ducks","volume":"318","author":"Pioche","year":"2020","journal-title":"Am. J. Physiol. Regul. Integr. Comp. Physiol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1186\/2251-6581-13-51","article-title":"Modulation of de novo purine biosynthesis leads to activation of AMPK and results in improved glucose handling and insulin sensitivity","volume":"13","author":"Sadasivan","year":"2014","journal-title":"J. Diabetes Metab. Disord."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"E901","DOI":"10.1152\/ajpendo.00087.2011","article-title":"Metabolic consequences of ENPP1 overexpression in adipose tissue","volume":"301","author":"Pan","year":"2011","journal-title":"Am. J. Physiol. Endocrinol. Metab."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"909","DOI":"10.1152\/physrev.00026.2013","article-title":"Mitochondrial reactive oxygen species (ROS) and ROS-induced ROS release","volume":"94","author":"Zorov","year":"2014","journal-title":"Physiol. Rev."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1977","DOI":"10.1194\/jlr.M085613","article-title":"Mitochondrial dysfunction-related lipid changes occur in nonalcoholic fatty liver disease progression","volume":"59","author":"Peng","year":"2018","journal-title":"J. Lipid Res."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"2661","DOI":"10.3748\/wjg.v24.i25.2661","article-title":"Apoptosis and non-alcoholic fatty liver diseases","volume":"24","author":"Kanda","year":"2018","journal-title":"World J. Gastroenterol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"skad059","DOI":"10.1093\/jas\/skad059","article-title":"Glucose-induced enhanced anti-oxidant activity inhibits apoptosis in goose fatty liver","volume":"101","author":"Sun","year":"2023","journal-title":"J. Anim. Sci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1080\/00071668708416953","article-title":"Contribution of lipoprotein lipase to differences in fatness between broiler and layer-strain chickens","volume":"28","author":"Griffin","year":"1987","journal-title":"Br. Poult. Sci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"9369","DOI":"10.1016\/S0021-9258(18)98359-6","article-title":"Low density lipoprotein receptor internalizes low density and very low density lipoproteins that are bound to heparan sulfate proteoglycans via lipoprotein lipase","volume":"268","author":"Mulder","year":"1993","journal-title":"J. Biol. Chem."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Wang, W., Li, W., Liu, W., Wang, Z., Xie, B., Yang, X., and Tang, Z. (2024). Exploring Multi-Tissue Alternative Splicing and Skeletal Muscle Metabolism Regulation in Obese- and Lean-Type Pigs. Genes, 15.","DOI":"10.3390\/genes15020196"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"2686","DOI":"10.1038\/s41436-019-0561-7","article-title":"Liver fibrosis during clinical ascertainment of glycogen storage disease type III: A need for improved and systematic monitoring","volume":"21","author":"Halaby","year":"2019","journal-title":"Genet. Med."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1016\/j.bbrc.2019.06.028","article-title":"MGARP is ultrastructurally located in the inner faces of mitochondrial membranes","volume":"516","author":"Zhang","year":"2019","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1293","DOI":"10.1007\/s12035-013-8602-8","article-title":"MGARP regulates mouse neocortical development via mitochondrial positioning","volume":"49","author":"Jia","year":"2014","journal-title":"Mol. Neurobiol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"100728","DOI":"10.1016\/j.drup.2020.100728","article-title":"The role of alternative splicing in cancer: From oncogenesis to drug resistance","volume":"53","author":"Sciarrillo","year":"2020","journal-title":"Drug Resist. Updat."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"2203","DOI":"10.3748\/wjg.v26.i18.2203","article-title":"Folic acid attenuates high-fat diet-induced steatohepatitis via deacetylase SIRT1-dependent restoration of PPAR\u03b1","volume":"26","author":"Xin","year":"2020","journal-title":"World J. Gastroenterol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1093\/carcin\/bgn288","article-title":"CCDC62\/ERAP75 functions as a coactivator to enhance estrogen receptor beta-mediated transactivation and target gene expression in prostate cancer cells","volume":"30","author":"Chen","year":"2009","journal-title":"Carcinogenesis"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"606","DOI":"10.1177\/1535370216688569","article-title":"An estrogen receptor \u03b2-selective agonist inhibits non-alcoholic steatohepatitis in preclinical models by regulating bile acid and xenobiotic receptors","volume":"242","author":"Ponnusamy","year":"2017","journal-title":"Exp. Biol. Med."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"5996","DOI":"10.1002\/alz.14062","article-title":"The MIND diet, brain transcriptomic alterations, and dementia","volume":"20","author":"Li","year":"2024","journal-title":"Alzheimers Dement."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"6430","DOI":"10.1158\/1078-0432.CCR-13-0550","article-title":"Germline variants and advanced colorectal adenomas: Adenoma prevention with celecoxib trial genome-wide association study","volume":"19","author":"Wang","year":"2013","journal-title":"Clin. Cancer Res."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1496992","DOI":"10.3389\/fimmu.2024.1496992","article-title":"Research progress of CD80 in the development of immunotherapy drugs","volume":"15","author":"Li","year":"2024","journal-title":"Front. Immunol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"558","DOI":"10.1126\/science.aav7062","article-title":"Restriction of PD-1 function by cis-PD-L1\/CD80 interactions is required for optimal T cell responses","volume":"364","author":"Sugiura","year":"2019","journal-title":"Science"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"e202551771","DOI":"10.1002\/eji.202551771","article-title":"Treg Control of CD80\/CD86 Expression Mediates Immune System Homeostasis","volume":"55","author":"Kim","year":"2025","journal-title":"Eur. J. Immunol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"505","DOI":"10.32598\/bcn.2024.6930.1","article-title":"Expanding the Phenotype and Genotype Spectrum of a Novel Mutation in Hypomyelinating Leukodystrophy-5 with a Review of the Literature on 42 Cases","volume":"16","author":"Bayat","year":"2025","journal-title":"Basic Clin. Neurosci."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Trybus, M., Hryniewicz-Jankowska, A., Czogalla, A., and Sikorski, A.F. (2025). EFR3A, an intriguing gene, and protein with a scaffolding function. Cells, 14.","DOI":"10.20944\/preprints202501.2282.v1"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"831","DOI":"10.1080\/17425255.2018.1492552","article-title":"Personalized therapy when tackling nonalcoholic fatty liver disease: A focus on sex, genes, and drugs","volume":"14","author":"Skubic","year":"2018","journal-title":"Expert Opin. Drug Metab. Toxicol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1291","DOI":"10.1007\/s12325-017-0556-1","article-title":"NAFLD as a Sexual Dimorphic Disease: Role of Gender and Reproductive Status in the Development and Progression of Nonalcoholic Fatty Liver Disease and Inherent Cardiovascular Risk","volume":"34","author":"Ballestri","year":"2017","journal-title":"Adv. Ther."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Atallah, E., Trehiou, S., Alquier-Bacquie, V., Lasserre, F., Arroyo, J., Molette, C., and Remignon, H. (2024). Development of hepatic steatosis in male and female mule ducks after respective force-feeding programs. Front. Physiol., 15.","DOI":"10.3389\/fphys.2024.1392968"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1016\/j.hbpd.2022.10.003","article-title":"Wnt\/beta-catenin signaling and its modulators in nonalcoholic fatty liver diseases","volume":"22","author":"Ezhilarasan","year":"2023","journal-title":"Hepatobiliary Pancreat. Dis. Int."},{"key":"ref_53","first-page":"2201","article-title":"Role and mechanisms of action of microRNA-21 as regards the regulation of the WNT\/\u03b2-catenin signaling pathway in the pathogenesis of non-alcoholic fatty liver disease","volume":"44","author":"Wang","year":"2019","journal-title":"Int. J. Mol. Med."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Nie, C., He, T., Zhang, W., Zhang, G., and Ma, X. (2018). Branched Chain Amino Acids: Beyond Nutrition Metabolism. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19040954"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"2311","DOI":"10.1210\/clinem\/dgaa175","article-title":"Plasma BCAA Changes in Patients With NAFLD Are Sex Dependent","volume":"105","author":"Grzych","year":"2020","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1111\/imr.13214","article-title":"The role of efferocytosis-fueled macrophage metabolism in the resolution of inflammation","volume":"319","author":"Schilperoort","year":"2023","journal-title":"Immunol. Rev."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"554","DOI":"10.1124\/dmd.112.048439","article-title":"Altered UDP-glucuronosyltransferase and sulfotransferase expression and function during progressive stages of human nonalcoholic fatty liver disease","volume":"41","author":"Hardwick","year":"2013","journal-title":"Drug Metab. Dispos."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1093\/toxsci\/kfj061","article-title":"Human sulfotransferases and their role in chemical metabolism","volume":"90","author":"Gamage","year":"2006","journal-title":"Toxicol. Sci."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1006\/abbi.2001.2368","article-title":"Structure and function of sulfotransferases","volume":"390","author":"Negishi","year":"2001","journal-title":"Arch. Biochem. Biophys."},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Naik, A., Beli\u010d, A., Zanger, U.M., and Rozman, D. (2013). Molecular Interactions between NAFLD and Xenobiotic Metabolism. Front. Genet., 4.","DOI":"10.3389\/fgene.2013.00002"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"103287","DOI":"10.1016\/j.redox.2024.103287","article-title":"The deubiquitinase OTUD1 stabilizes NRF2 to alleviate hepatic ischemia\/reperfusion injury","volume":"75","author":"Zhang","year":"2024","journal-title":"Redox Biol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"694","DOI":"10.1038\/s41419-022-05145-5","article-title":"OTUD1 deubiquitinase regulates NF-\u03baB- and KEAP1-mediated inflammatory responses and reactive oxygen species-associated cell death pathways","volume":"13","author":"Oikawa","year":"2022","journal-title":"Cell Death Dis."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"26","DOI":"10.3164\/jcbn.11-005FR","article-title":"Lipoic acid: Energy metabolism and redox regulation of transcription and cell signaling","volume":"48","author":"Packer","year":"2011","journal-title":"J. Clin. Biochem. Nutr."},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"McDermott-Roe, C., Leleu, M., Rowe, G.C., Palygin, O., Bukowy, J.D., Kuo, J., Rech, M., Hermans-Beijnsberger, S., Schaefer, S., and Adami, E. (2017). Transcriptome-wide co-expression analysis identifies LRRC2 as a novel mediator of mitochondrial and cardiac function. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0170458"},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Bai, D.P., Chen, Y., Hu, Y.Q., He, W.F., Shi, Y.Z., Fan, Q.M., Luo, R.T., and Li, A. (2020). Transcriptome analysis of genes related to gonad differentiation and development in Muscovy ducks. BMC Genom., 21.","DOI":"10.1186\/s12864-020-06852-z"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"2001","DOI":"10.1007\/s00018-013-1484-0","article-title":"Changes in the expression of the glutamate transporter EAAT3\/EAAC1 in health and disease","volume":"71","author":"Bianchi","year":"2014","journal-title":"Cell. Mol. Life Sci."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1038\/s41597-024-03567-8","article-title":"Telomere-to-telomere genome assembly of the goose Anser cygnoides","volume":"11","author":"Zhao","year":"2024","journal-title":"Sci. Data"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"907","DOI":"10.1038\/s41587-019-0201-4","article-title":"Graph-based genome alignment and genotyping with HISAT2 and HISAT-genotype","volume":"37","author":"Kim","year":"2019","journal-title":"Nat. Biotechnol."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"2078","DOI":"10.1093\/bioinformatics\/btp352","article-title":"The Sequence Alignment\/Map format and SAMtools","volume":"25","author":"Li","year":"2009","journal-title":"Bioinformatics"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2","volume":"15","author":"Love","year":"2014","journal-title":"Genome Biol."},{"key":"ref_71","doi-asserted-by":"crossref","unstructured":"Langfelder, P., and Horvath, S. (2008). WGCNA: An R package for weighted correlation network analysis. BMC Bioinform., 9.","DOI":"10.1186\/1471-2105-9-559"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"2498","DOI":"10.1101\/gr.1239303","article-title":"Cytoscape: A software environment for integrated models of biomolecular interaction networks","volume":"13","author":"Shannon","year":"2003","journal-title":"Genome Res."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1038\/nprot.2008.73","article-title":"Analyzing real-time PCR data by the comparative C(T) method","volume":"3","author":"Schmittgen","year":"2008","journal-title":"Nat. Protoc."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/1\/407\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,6]],"date-time":"2026-01-06T05:12:42Z","timestamp":1767676362000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/1\/407"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,30]]},"references-count":73,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2026,1]]}},"alternative-id":["ijms27010407"],"URL":"https:\/\/doi.org\/10.3390\/ijms27010407","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12,30]]}}}