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Mice in which the SATB1 gene is conditionally deleted in hematopoietic cells (SATB1cKO mice) develop SS as early as 4 weeks of age; however, the etiology of the disease remains to be elucidated. Here, we found that the frequency of abnormally appearing CD4+CD8+ double positive (DP) T cells in the periphery of SATB1cKO mice was higher in the salivary glands than that in the spleen, suggesting a possible involvement of DP T cells in the pathogenesis of SS in SATB1cKO mice. To investigate the nature of DP T cells, we established DP T cell hybridomas by fusing T cells from the cervical lymph nodes of SATB1cKO mice with the BW5147 thymoma cell line. Among six DP hybridoma clones, the TCR\u03b2 gene from five clones exhibited a fetal or immature phenotype. In addition, four out of five clones exhibited upregulated transcription of IL-2 in the salivary glands of T\/B cell-deficient RAG2\u2212\/\u2212 mice, suggesting that autoreactive T cells were enriched in the DP T cell population of SATB1cKO mice. These results suggest that unusual DP T cells in SATB1cKO mice may be involved in autoimmune pathogenesis in SATB1cKO mice.<\/jats:p>","DOI":"10.3390\/ijms27010414","type":"journal-article","created":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T15:30:32Z","timestamp":1767195032000},"page":"414","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Establishment of Autoreactive CD4+CD8+ T Cell Hybridomas from Sj\u00f6gren\u2019s Disease Model, SATB1 Conditional Knockout Mice"],"prefix":"10.3390","volume":"27","author":[{"given":"Shuhei","family":"Mashimo","sequence":"first","affiliation":[{"name":"Department of Molecular Immunology, Toho University School of Medicine, Tokyo 143-8540, Japan"},{"name":"Department of Dermatology, Toho University Omori Medical Center, Tokyo 143-8541, Japan"},{"name":"Department of Dermatology, Toho University Graduate School of Medicine, Tokyo 143-8540, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michitsune","family":"Arita","sequence":"additional","affiliation":[{"name":"Department of Molecular Immunology, Toho University School of Medicine, Tokyo 143-8540, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5985-8245","authenticated-orcid":false,"given":"Taku","family":"Kuwabara","sequence":"additional","affiliation":[{"name":"Department of Molecular Immunology, Toho University School of Medicine, Tokyo 143-8540, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taku","family":"Naito","sequence":"additional","affiliation":[{"name":"Department of Molecular Immunology, Toho University School of Medicine, Tokyo 143-8540, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sakurako","family":"Takizawa","sequence":"additional","affiliation":[{"name":"Department of Molecular Immunology, Toho University School of Medicine, Tokyo 143-8540, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5097-7811","authenticated-orcid":false,"given":"Akiko","family":"Inoue","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology, Toho University Omori Medical Center, Tokyo 143-8541, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1163-870X","authenticated-orcid":false,"given":"Akira","family":"Ishiko","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Toho University Omori Medical Center, Tokyo 143-8541, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1565-8804","authenticated-orcid":false,"given":"Motonari","family":"Kondo","sequence":"additional","affiliation":[{"name":"Department of Molecular Immunology, Toho University School of Medicine, Tokyo 143-8540, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9278-7677","authenticated-orcid":false,"given":"Yuriko","family":"Tanaka","sequence":"additional","affiliation":[{"name":"Department of Molecular Immunology, Toho University School of Medicine, Tokyo 143-8540, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,12,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1016\/S0140-6736(05)66990-5","article-title":"Sj\u00f6gren\u2019s Syndrome","volume":"366","author":"Fox","year":"2005","journal-title":"Lancet"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1038\/s41584-025-01268-z","article-title":"2023 International Rome Consensus for the Nomenclature of Sj\u00f6gren Disease","volume":"21","author":"Baer","year":"2025","journal-title":"Nat. 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