{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T21:56:54Z","timestamp":1790200614002,"version":"4.1.0"},"reference-count":51,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T00:00:00Z","timestamp":1768435200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"CONICYT-FONDAP","award":["15130011"],"award-info":[{"award-number":["15130011"]}]},{"name":"FONDAP Continuation Project","award":["1523A0008"],"award-info":[{"award-number":["1523A0008"]}]},{"name":"ANID-FONDECYT-Regular","award":["1170925"],"award-info":[{"award-number":["1170925"]}]},{"name":"ANID-FONDECYT-Regular","award":["1210644"],"award-info":[{"award-number":["1210644"]}]},{"name":"ANID-FONDECYT-Regular","award":["1251598"],"award-info":[{"award-number":["1251598"]}]},{"name":"ANID-FONDECYT-Regular","award":["1240295"],"award-info":[{"award-number":["1240295"]}]},{"name":"ANID-FONDECYT-Regular","award":["1200951"],"award-info":[{"award-number":["1200951"]}]},{"name":"ANID-FONDECYT Iniciaci\u00f3n","award":["11251459"],"award-info":[{"award-number":["11251459"]}]},{"name":"VRIP-UBB","award":["GI2301146"],"award-info":[{"award-number":["GI2301146"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Thrombotic cardiovascular diseases are frequent side effects of cancer therapy with cytotoxic drugs such as Doxorubicin. Endothelial cell senescence is emerging as a critical mechanism underlying endothelial dysfunction in this context. Senescent cells, although unable to proliferate, secrete bioactive molecules that alter the tissue microenvironment, a feature known as the senescence-associated secretory phenotype (SASP). Besides soluble molecules, senescent cells also release extracellular vesicles (EVs). Previous studies indicate that senescent endothelial cells produce a secretome that promotes platelet activation; however, the contribution of EVs remains unclear. Here, we show that human microvascular endothelial cells (HMEC-1) exposed to Doxorubicin undergo senescence, display endothelial dysfunction, and release EVs. We found no differences in the concentration or size distribution of EVs from senescent and non-senescent cells. Nevertheless, EVs from senescent HMEC-1 promoted platelet activation more strongly than EVs from control cells. Notably, EVs alone did not induce platelet aggregation, suggesting that soluble factors are also required to support platelet-dependent hemostasis. These findings reveal that EVs from senescent endothelial cells contribute to platelet activation, a process that may favor thrombosis in patients receiving Doxorubicin-based chemotherapy.<\/jats:p>","DOI":"10.3390\/ijms27020869","type":"journal-article","created":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T12:31:41Z","timestamp":1768480301000},"page":"869","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Extracellular Vesicles (EVs) Derived from Senescent Endothelial Cells Promote Platelet Activation"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1911-6006","authenticated-orcid":false,"given":"Whitney","family":"Venturini","sequence":"first","affiliation":[{"name":"Departamento de Medicina Traslacional, Facultad de Medicina, Universidad Cat\u00f3lica del Maule, Talca 3460000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-7689-7380","authenticated-orcid":false,"given":"Angel","family":"Cayo","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Traslacional, Facultad de Medicina, Universidad Cat\u00f3lica del Maule, Talca 3460000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7647-5198","authenticated-orcid":false,"given":"Gabriel","family":"Diaz-Serrano","sequence":"additional","affiliation":[{"name":"Faculty of Health Sciences, University of Talca, Talca 3460000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sofia","family":"Sanhueza","sequence":"additional","affiliation":[{"name":"Cell Communication Laboratory, Center for Studies on Exercise, Metabolism and Cancer (CEMC), Institute of Biomedical Sciences (ICBM), Faculty of Medicine, University of Chile, Santiago 8380453, Chile"},{"name":"Advanced 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Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danitza","family":"Rebolledo-Mira","sequence":"additional","affiliation":[{"name":"Faculty of Health Sciences, University of Talca, Talca 3460000, Chile"},{"name":"Center for Medical Research, Medical School, University of Talca, Talca 3460000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catalina","family":"Silva-Pereira","sequence":"additional","affiliation":[{"name":"Faculty of Health Sciences, University of Talca, Talca 3460000, Chile"},{"name":"Center for Medical Research, Medical School, University of Talca, Talca 3460000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisca","family":"Torres-Orellana","sequence":"additional","affiliation":[{"name":"Faculty of Health Sciences, University of Talca, Talca 3460000, Chile"},{"name":"Center for Medical Research, Medical School, University of Talca, Talca 3460000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felipe","family":"Troncoso","sequence":"additional","affiliation":[{"name":"Vascular Physiology Laboratory, Group of Research and Innovation in Vascular Health (GRIVAS Health), Basic Sciences Department, Faculty of Sciences, Universidad del Bio-Bio, Chillan 3780000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7688-4621","authenticated-orcid":false,"given":"Carlos","family":"Escudero","sequence":"additional","affiliation":[{"name":"Vascular Physiology Laboratory, Group of Research and Innovation in Vascular Health (GRIVAS Health), Basic Sciences Department, Faculty of Sciences, Universidad del Bio-Bio, Chillan 3780000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0099-4108","authenticated-orcid":false,"given":"Eduardo","family":"Fuentes","sequence":"additional","affiliation":[{"name":"MIBI: Interdisciplinary Group on Mitochondrial Targeting and Bioenergetics, Department of Clinical Biochemistry and Immunohematology, Thrombosis Research Center, Medical Technology School, Faculty of Health Sciences, Universidad de Talca, Talca 3460000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8755-1590","authenticated-orcid":false,"given":"Andrew F. G.","family":"Quest","sequence":"additional","affiliation":[{"name":"Cell Communication Laboratory, Center for Studies on Exercise, Metabolism and Cancer (CEMC), Institute of Biomedical Sciences (ICBM), Faculty of Medicine, University of Chile, Santiago 8380453, Chile"},{"name":"Advanced Center for Chronic Diseases (ACCDIS), Faculty of Medicine, University of Chile, Santiago 8380453, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudio","family":"Valenzuela","sequence":"additional","affiliation":[{"name":"Center for Medical Research, Medical School, University of Talca, Talca 3460000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2442-7514","authenticated-orcid":false,"given":"Juan C. 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