{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T02:00:18Z","timestamp":1768960818557,"version":"3.49.0"},"reference-count":87,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T00:00:00Z","timestamp":1768867200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"China agriculture research system of MOF and MARA","award":["CARS-46"],"award-info":[{"award-number":["CARS-46"]}]},{"name":"Sichuan freshwater fish innovation team of the National Modern Agricultural Industrial Technology System"},{"name":"Sichuan Science and Technology Planning Project","award":["2021YFYZ0015"],"award-info":[{"award-number":["2021YFYZ0015"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Body color is the most prominent phenotypic trait in animals. To investigate the molecular regulatory mechanisms underlying skin pigmentation and body color in Channa argus, single-nucleus RNA sequencing technology was employed to analyze cell diversity and functional changes in the skin of normal and albino C. argus. Three pigment-related cell types, seven immune-related cell types, and nine other skin-related structural and functional cell types were identified. The skin of albino C. argus, which appears white to the naked eye, contains numerous melanocytes and iridophores with reflective silver properties. Compared to normal C. argus, melanocytes in albino individuals contained fewer melanin granules, while iridophores exhibited increased chromogenic substances. Melanocyte-specific genes\u2014kitlg, myo5a, and scarb1\u2014were significantly downregulated in albino melanocytes (p &lt; 0.05). Conversely, iridophore-specific genes alk, pnp, and gpnmb were significantly upregulated in albino skin, whereas mlph was significantly downregulated (p &lt; 0.05). Weighted gene co-expression network analysis revealed that scarb1 was associated with the melanocyte module, alk was identified as a core gene, and pnp was linked to the iridophore module. Functionally, scarb1 is involved in pigment transport, pnp in purine synthesis, and alk is essential for iridophore development. Therefore, scarb1, pnp, and alk may be correlated to albinism in C. argus. Overall, this study constructed a single-cell transcriptional atlas of C. argus skin, providing valuable reference data for further research into the regulatory mechanisms governing body color formation and maintenance in this species.<\/jats:p>","DOI":"10.3390\/ijms27021023","type":"journal-article","created":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T13:02:07Z","timestamp":1768914127000},"page":"1023","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Single-Nucleus Transcriptional Profiling Revealed Cell Diversity and Albino Mutation Mechanism in the Skin of Channa argus"],"prefix":"10.3390","volume":"27","author":[{"given":"Lu","family":"Zhang","sequence":"first","affiliation":[{"name":"Fisheries Research Institute, Sichuan Academy of Agricultural Sciences (Sichuan Fisheries Research Institute), Chengdu 611731, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6718-2724","authenticated-orcid":false,"given":"Jian","family":"Zhou","sequence":"additional","affiliation":[{"name":"Fisheries Research Institute, Sichuan Academy of Agricultural Sciences (Sichuan Fisheries Research Institute), Chengdu 611731, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiang","family":"Li","sequence":"additional","affiliation":[{"name":"Fisheries Research Institute, Sichuan Academy of Agricultural Sciences (Sichuan Fisheries Research Institute), Chengdu 611731, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongyu","family":"Ke","sequence":"additional","affiliation":[{"name":"Fisheries Research Institute, Sichuan Academy of Agricultural Sciences (Sichuan Fisheries Research Institute), Chengdu 611731, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhipeng","family":"Huang","sequence":"additional","affiliation":[{"name":"Fisheries Research Institute, Sichuan Academy of Agricultural Sciences (Sichuan Fisheries Research Institute), Chengdu 611731, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4948-4715","authenticated-orcid":false,"given":"Zhongmeng","family":"Zhao","sequence":"additional","affiliation":[{"name":"Fisheries Research Institute, Sichuan Academy of Agricultural Sciences (Sichuan Fisheries Research Institute), Chengdu 611731, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Han","family":"Zhao","sequence":"additional","affiliation":[{"name":"Fisheries Research Institute, Sichuan Academy of Agricultural Sciences (Sichuan Fisheries Research Institute), Chengdu 611731, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chengyan","family":"Mou","sequence":"additional","affiliation":[{"name":"Fisheries Research Institute, Sichuan Academy of Agricultural Sciences (Sichuan Fisheries Research Institute), Chengdu 611731, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Fan","sequence":"additional","affiliation":[{"name":"Neijiang Academy of Agricultural Sciences, Neijiang 641000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuanliang","family":"Duan","sequence":"additional","affiliation":[{"name":"Fisheries Research Institute, Sichuan Academy of Agricultural Sciences (Sichuan Fisheries Research Institute), Chengdu 611731, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,1,20]]},"reference":[{"key":"ref_1","first-page":"54","article-title":"Industrialized breeding technology for Channa argus fingerlings","volume":"41","author":"Wang","year":"2020","journal-title":"J. Aquac."},{"key":"ref_2","unstructured":"Bureau of Fisheries and Fishery Administration, Ministry of Agriculture and Rural Affairs, National Fisheries Technology Extension Center, and China Society of Fisheries (2025). China Fishery Statistical Yearbook, (In Chinese)."},{"key":"ref_3","unstructured":"Wang, J., Zhao, X., Zhou, C., and Liao, Z. (1992). Comparative studies on the Ophiocephalus argus and O. A. kimurai, with the systematics of Ophiocephalus argus. Trans. Oceanol. Limnol., 51\u201357. (In Chinese)."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1591","DOI":"10.17582\/journal.pjz\/20191219001209","article-title":"The species identity of the two color morphs of northern snakehead (Channa argus) based on mtDNA control region sequences","volume":"53","author":"Zhou","year":"2021","journal-title":"Pak. J. Zool."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1080\/23802359.2022.2029601","article-title":"Genetic diversity of two color morphs of northern snakehead (Channa argus) unveiled by the mitochondrial DNA D-loop region","volume":"7","author":"Fan","year":"2022","journal-title":"Mitochondrial DNA Part B"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"e60388","DOI":"10.7554\/eLife.60388","article-title":"Regulation of dynamic pigment cell states at single-cell resolution","volume":"9","author":"Perillo","year":"2020","journal-title":"eLife"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1007\/s004380100464","article-title":"Genetic mapping of the dominant albino locus in rainbow trout (Oncorhynchus mykiss)","volume":"265","author":"Nakamura","year":"2001","journal-title":"Mol. Genet. Genom."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Zou, M., Zhang, X., Shi, Z., Lin, L., Ouyang, G., Zhang, G., Zheng, H., Wei, K., and Ji, W. (2015). A comparative transcriptome analysis between wild and albino yellow catfish (Pelteobagrus fulvidraco). PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0131504"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"R1014","DOI":"10.1016\/j.cub.2021.06.016","article-title":"Evolution: The genetics of trait evolution in cavefish","volume":"31","author":"Borowsky","year":"2021","journal-title":"Curr. Biol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1034\/j.1600-0749.2003.00072.x","article-title":"Quantitative analysis of eumelanin and pheomelanin in humans, mice, and other animals: A comparative review","volume":"16","author":"Ito","year":"2003","journal-title":"Pigment Cell Res."},{"key":"ref_11","first-page":"18","article-title":"Preliminary analyse of the pathogeny of albinism in fish","volume":"27","author":"Wang","year":"2003","journal-title":"Mar. Sci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1186\/s13059-021-02493-x","article-title":"Epigenetic dynamics shaping melanophore and iridophore cell fate in zebrafish","volume":"22","author":"Jang","year":"2021","journal-title":"Genome Biol."},{"key":"ref_13","first-page":"79","article-title":"De novo transcriptome analysis and comparison of the FFRC No.2 strain common carp (Cyprinus carpio) associated with its muscle growth","volume":"45","author":"Wang","year":"2021","journal-title":"J. Fish. China"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1006\/dbio.1995.1004","article-title":"Genetic control of adult pigment stripe development in zebrafish","volume":"167","author":"Johnson","year":"1995","journal-title":"Dev. Biol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1006\/dbio.2001.0418","article-title":"How the zebrafish gets its stripes","volume":"240","author":"Rawls","year":"2001","journal-title":"Dev. Biol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2997","DOI":"10.1242\/dev.096719","article-title":"Iridophores and their interactions with other chromatophores are required for stripe formation in zebrafish","volume":"140","author":"Krauss","year":"2013","journal-title":"Development"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Braasch, I., Schartl, M., and Volff, J.N. (2007). Evolution of pigment synthesis pathways by gene and genome duplication in fish. BMC Evol. Biol., 7.","DOI":"10.1186\/1471-2148-7-74"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Nikaido, M., Subkhankulova, T., Uroshlev, L.A., Kasianov, A.J., Sosa, K.C., Bavister, G., Yang, X., Rodrigues, F.S., Carney, T.J., and Schwetlick, H. (2021). Zebrafish pigment cells develop directly from persistent highly multipotent progenitors. bioRxiv.","DOI":"10.1101\/2021.06.17.448805"},{"key":"ref_19","first-page":"206","article-title":"Mechanisms underlying carotenoid-based coloration in fishes","volume":"27","author":"Li","year":"2018","journal-title":"J. Shanghai Ocean Univ."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"750","DOI":"10.14348\/molcells.2016.0173","article-title":"Innate color preference of zebrafish and its use in behavioral analyses","volume":"39","author":"Park","year":"2016","journal-title":"Mol. Cells"},{"key":"ref_21","first-page":"849","article-title":"Identification of mutation sites related to body color albinism in Cynoglossus semilaevis","volume":"31","author":"Sun","year":"2022","journal-title":"J. Shanghai Ocean Univ."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"979","DOI":"10.1111\/jfb.13556","article-title":"Molecular cloning and expression analysis of tyr and tyrp1 genes in normal and albino yellow catfish Tachysurus fulvidraco","volume":"92","author":"Zhang","year":"2018","journal-title":"J. Fish Biol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1007\/s003350020017","article-title":"Mutations in the agouti (ASIP), the extension (MC1R), and the brown (TYRP1) loci and their association to coat color phenotypes in horses (Equus caballus)","volume":"12","author":"Rieder","year":"2001","journal-title":"Mamm. Genome"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2775","DOI":"10.4238\/2015.March.31.7","article-title":"Involvement of the mitfa gene in the development of pigment cell in Japanese ornamental (Koi) carp (Cyprinus carpio L.)","volume":"14","author":"Liu","year":"2015","journal-title":"Genet. Mol. Res."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"3396","DOI":"10.1128\/MCB.24.8.3396-3403.2004","article-title":"Melanocytes and pigmentation are affected in dopachrome tautomerase knockout mice","volume":"24","author":"Guyonneau","year":"2004","journal-title":"Mol. Cell. Biol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"4827","DOI":"10.1242\/dev.115584","article-title":"Precise and efficient genome editing in zebrafish using the CRISPR\/Cas9 system","volume":"141","author":"Irion","year":"2014","journal-title":"Development"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1007\/s00438-017-1302-8","article-title":"A deletion in the Hermansky\u2013Pudlak syndrome 4 (Hps4) gene appears to be responsible for albinism in channel catfish","volume":"292","author":"Li","year":"2017","journal-title":"Mol. Genet. Genom."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1111\/pcmr.12205","article-title":"oca2 regulation of chromatophore differentiation and number is cell type specific in zebrafish","volume":"27","author":"Beirl","year":"2014","journal-title":"Pigment Cell Melanoma Res."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"572","DOI":"10.1007\/s11626-015-9871-5","article-title":"Transcriptome analysis reveals the potential mechanism of the albino skin development in pufferfish Takifugu obscurus","volume":"51","author":"Jin","year":"2015","journal-title":"In Vitro Cell. Dev. Biol.-Anim."},{"key":"ref_30","first-page":"715","article-title":"Exploration of Molecular Mechanism of Skin Albinism in Albino Northern Snakehead Channa argus var. (Teleostei: Channidae) Based on Transcriptome Sequencing","volume":"41","author":"Li","year":"2022","journal-title":"Fish. Sci."},{"key":"ref_31","first-page":"405","article-title":"Application of single cell transcriptome sequencing technology in livestock production","volume":"42","author":"Xia","year":"2022","journal-title":"Chin. J. Vet. Sci."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Zhou, A., Xie, S., Feng, Y., Sun, D., Liu, S., Sun, Z., Li, M., Zhang, C., and Zou, J. (2020). Insights into the albinism mechanism for two distinct color morphs of Northern Snakehead, Channa argus through histological and transcriptome analyses. Front. Genet., 11.","DOI":"10.3389\/fgene.2020.00830"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1038\/s41587-020-0465-8","article-title":"Systematic comparison of single-cell and sin-gle-nucleus RNA-sequencing methods","volume":"38","author":"Ding","year":"2020","journal-title":"Nat. Biotechnol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1038\/s41596-022-00752-0","article-title":"Optimized single-nucleus transcriptional profiling by combinatorial indexing","volume":"18","author":"Martin","year":"2023","journal-title":"Nat. Protoc."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1038\/s41586-020-2157-4","article-title":"Construction of a human cell landscape at single-cell level","volume":"581","author":"Han","year":"2020","journal-title":"Nature"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1091","DOI":"10.1016\/j.cell.2018.02.001","article-title":"Mapping the mouse cell atlas by microwell-seq","volume":"172","author":"Han","year":"2018","journal-title":"Cell"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"e45181","DOI":"10.7554\/eLife.45181","article-title":"Thyroid hormone regulates distinct paths to maturation in pigment cell lineages","volume":"8","author":"Saunders","year":"2019","journal-title":"eLife"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"12027","DOI":"10.1073\/pnas.1710254114","article-title":"Alk and Ltk ligands are essential for iridophore development in zebrafish mediated by the receptor tyrosine kinase Ltk","volume":"114","author":"Mo","year":"2017","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Nagao, Y., Takada, H., Miyadai, M., Adachi, T., Seki, R., Kamei, Y., Hara, I., Taniguchi, Y., Naruse, K., and Hibi, M. (2018). Distinct interactions of Sox5 and Sox10 in fate specification of pigment cells in medaka and zebrafish. PLoS Genet., 14.","DOI":"10.1371\/journal.pgen.1007260"},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Bannasch, D.L., Affolter, V.K., York, D., Rebhun, R.B., Grahn, R.A., Weich, K.M., and Kallenberg, A. (2020). Pigment intensity in dogs is associated with a copy number variant upstream of KITLG. Genes, 11, Erratum in Genes 2021, 12, 357. https:\/\/doi.org\/10.3390\/genes12030357.","DOI":"10.3390\/genes12030357"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1038\/s42003-020-0922-4","article-title":"Single-cell transcriptomes of the human skin reveal age-related loss of fibroblast priming","volume":"3","author":"Raddatz","year":"2020","journal-title":"Commun. Biol."},{"key":"ref_42","first-page":"117","article-title":"Overview of fish pigment cells and the ecological significance","volume":"36","author":"Yu","year":"2020","journal-title":"J. Guangxi Acad. Sci."},{"key":"ref_43","first-page":"30","article-title":"Research progress on color-related genes of fish","volume":"32","author":"Meng","year":"2020","journal-title":"J. Hunan Univ. Arts Sci. (Sci. Technol.)"},{"key":"ref_44","first-page":"11969","article-title":"Melanogenesis, its regulatory process, and insights on biomedical, biotechnological, and pharmacological potentials of melanin as antiviral biochemical","volume":"11","author":"Fatoki","year":"2021","journal-title":"Biointerface Res. Appl. Chem."},{"key":"ref_45","first-page":"100944","article-title":"Distinct skin morphological and transcriptomic profiles between wild and albino Oscar Astronotus ocellatus","volume":"41","author":"Wang","year":"2022","journal-title":"Comp. Biochem. Physiol. Part D Genom. Proteom."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Lee, S.Y., and Lee, H.J. (2020). Comprehensive RNA-seq analysis to evaluate the pigmentation-related genes involved in albinism of cichlid fish, Aulonocara baenschi. Front. Mar. Sci., 7.","DOI":"10.3389\/fmars.2020.00723"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1111\/pcmr.12568","article-title":"An ongoing role for Wnt signaling in differentiating melanocytes In Vivo","volume":"30","author":"Vibert","year":"2017","journal-title":"Pigment Cell Melanoma Res."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"4113","DOI":"10.1242\/dev.128.21.4113","article-title":"Zebrafish colourless encodes sox10 and specifies non-ectomesenchymal neural crest fates","volume":"128","author":"Dutton","year":"2001","journal-title":"Development"},{"key":"ref_49","first-page":"545","article-title":"Microscopic observation of chromatophores in skin, scale, and fin of mandarinfish Siniperca chuatsi","volume":"39","author":"Wu","year":"2020","journal-title":"Fish. Sci."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1186\/s13059-023-02887-z","article-title":"Genetic mapping and molecular mechanism behind color variation in the Asian vine snake","volume":"24","author":"Tang","year":"2023","journal-title":"Genome Biol."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Higdon, C.W., Mitra, R.D., and Johnson, S.L. (2013). Gene expression analysis of zebrafish melanocytes, iridophores, and retinal pigmented epithelium reveals indicators of biological function and developmental origin. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0067801"},{"key":"ref_52","first-page":"24","article-title":"Advances in the research of pigment cell lineage and its developmental mechanism","volume":"37","author":"Wen","year":"2014","journal-title":"J. Nat. Sci. Hunan Norm. Univ."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/j.ydbio.2010.04.023","article-title":"Interplay between Foxd3 and Mitf regulates cell fate plasticity in the zebrafish neural crest","volume":"344","author":"Curran","year":"2010","journal-title":"Dev. Biol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"3757","DOI":"10.1242\/dev.126.17.3757","article-title":"nacre encodes a zebrafish microphthalmia-related protein that regulates neural-crest-derived pigment cell fate","volume":"126","author":"Lister","year":"1999","journal-title":"Development"},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Petratou, K., Subkhankulova, T., Lister, J.A., Rocco, A., Schwetlick, H., and Kelsh, R.N. (2018). A systems biology approach uncovers the core gene regulatory network governing iridophore fate choice from the neural crest. PLoS Genet., 14.","DOI":"10.1101\/318402"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"e21261","DOI":"10.1096\/fj.201903141RR","article-title":"Novel mutations in the Myo5a gene cause a dilute coat color phenotype in mice","volume":"35","author":"Zhang","year":"2021","journal-title":"FASEB J."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"985","DOI":"10.1016\/j.jid.2016.11.026","article-title":"MYO5A gene is a target of MITF in melanocytes","volume":"137","author":"Alves","year":"2017","journal-title":"J. Investig. Dermatol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"E127","DOI":"10.1086\/667861","article-title":"The vitamin A\u2013redox hypothesis: A biochemical basis for honest signaling via carotenoid pigmentation","volume":"180","author":"Hill","year":"2012","journal-title":"Am. Nat."},{"key":"ref_59","unstructured":"Xu, Q. (2022). Preliminary Study on Correlation of Scarb1 to Body Color Phenotype of Oujiang Color Common Carp. [Master\u2019s Thesis, Shanghai Ocean University]. (In Chinese)."},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Sundvold, H., Helgeland, H., Baranski, M., Omholt, S.W., and V\u00e5ge, D.I. (2011). Characterisation of a novel paralog of scavenger receptor class B member I (SCARB1) in Atlantic salmon (Salmo salar). BMC Genet., 12.","DOI":"10.1186\/1471-2156-12-52"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"737334","DOI":"10.1016\/j.aquaculture.2021.737334","article-title":"microRNA-430b targets scavenger receptor class B member 1 (scarb1) and inhibits coloration and carotenoid synthesis in koi carp (Cyprinus carpio L.)","volume":"546","author":"Tian","year":"2022","journal-title":"Aquaculture"},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Du, J. (2019). The Regulation Mechanism of Scarb1 and Scarb1-Like Genes in Controlling Red Coloration in Oujiang Color Common Carp (Cyprinus carpio var. Color). [Ph.D. Thesis, Shanghai Ocean University]. (In Chinese).","DOI":"10.1007\/s10695-018-0551-8"},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Kinslechner, K., Sch\u00fctz, B., Pistek, M., Rapolter, P., Weitzenb\u00f6ck, H.P., Hundsberger, H., Mikulits, W., Grillari, J., R\u00f6hrl, C., and Hengstschl\u00e4ger, M. (2019). Loss of SR-BI down-regulates MITF and suppresses extracellular vesicle release in human melanoma. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20051063"},{"key":"ref_64","first-page":"1965","article-title":"Development of pigment cells and analysis of the expression of related genes in the early embryo of Oryzias curvinotus","volume":"45","author":"Huang","year":"2021","journal-title":"J. Fish. China"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1016\/S0163-7258(00)00097-8","article-title":"Purine nucleoside phosphorylases: Properties, functions, and clinical aspects","volume":"88","author":"Bzowska","year":"2000","journal-title":"Pharmacol. Ther."},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Keebaugh, A.C., Mitchell, H.A., Gaval-Cruz, M., Freeman, K.G., Edwards, G.L., Weinshenker, D., and Thomas, J.W. (2011). PRTFDC1 is a genetic modifier of HPRT-deficiency in the mouse. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0022381"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"7921","DOI":"10.1038\/sj.onc.1210589","article-title":"PRTFDC1, a possible tumor-suppressor gene, is frequently silenced in oral squamous-cell carcinomas by aberrant promoter hypermethylation","volume":"26","author":"Suzuki","year":"2007","journal-title":"Oncogene"},{"key":"ref_68","first-page":"279","article-title":"Transcriptome analysis of bone marrow mesenchymal stem cells osteogenic induction in chicken","volume":"59","author":"Wang","year":"2023","journal-title":"Chin. J. Anim. Sci."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1038\/ng.106","article-title":"SLC2A9 is a newly identified urate transporter influencing serum urate concentration, urate excretion and gout","volume":"40","author":"Vitart","year":"2008","journal-title":"Nat. Genet."},{"key":"ref_70","first-page":"1681","article-title":"Novel genomic profiling identifies copy number changes in childhood leukemia","volume":"68","author":"Schiffman","year":"2008","journal-title":"Cancer Res."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"107185","DOI":"10.1016\/j.intimp.2020.107185","article-title":"AHNAK2 is a novel prognostic marker and correlates with immune infiltration in papillary thyroid cancer: Evidence from integrated analysis","volume":"90","author":"Zheng","year":"2021","journal-title":"Int. Immunopharmacol."},{"key":"ref_72","doi-asserted-by":"crossref","unstructured":"Wang, Q., Peng, C., Yang, M., Huang, F., Duan, X., Wang, S., Cheng, H., Yang, H., Zhao, H., and Qin, Q. (2021). Single-cell RNA-seq landscape midbrain cell responses to red spotted grouper nervous necrosis virus infection. PLoS Pathog., 17.","DOI":"10.1371\/journal.ppat.1009665"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1186\/s13059-019-1662-y","article-title":"EmptyDrops: Distinguishing cells from empty droplets in droplet-based single-cell RNA sequencing data","volume":"20","author":"Lun","year":"2019","journal-title":"Genome Biol."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1038\/nbt.4096","article-title":"Integrating single-cell transcriptomic data across different conditions, technologies, and species","volume":"36","author":"Butler","year":"2018","journal-title":"Nat. Biotechnol."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1016\/j.cels.2019.03.003","article-title":"DoubletFinder: Doublet detection in single-cell RNA sequencing data using artificial nearest neighbors","volume":"8","author":"McGinnis","year":"2019","journal-title":"Cell Syst."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"1289","DOI":"10.1038\/s41592-019-0619-0","article-title":"Fast, sensitive and accurate integration of single-cell data with Harmony","volume":"16","author":"Korsunsky","year":"2019","journal-title":"Nat. Methods"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"D721","DOI":"10.1093\/nar\/gky900","article-title":"CellMarker: A manually curated resource of cell markers in human and mouse","volume":"47","author":"Zhang","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"ref_78","first-page":"baz046","article-title":"PanglaoDB: A web server for exploration of mouse and human single-cell RNA sequencing data","volume":"2019","author":"Gan","year":"2019","journal-title":"Database"},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1038\/nature22796","article-title":"Multilineage communication regulates human liver bud development from pluripotency","volume":"546","author":"Camp","year":"2017","journal-title":"Nature"},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1038\/75556","article-title":"Gene ontology: Tool for the unification of biology","volume":"25","author":"Ashburner","year":"2000","journal-title":"Nat. Genet."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1093\/nar\/28.1.27","article-title":"KEGG: Kyoto encyclopedia of genes and genomes","volume":"28","author":"Kanehisa","year":"2000","journal-title":"Nucleic Acids Res."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"5170","DOI":"10.1016\/j.ygeno.2020.09.031","article-title":"Single-cell RNA-seq reveals different subsets of non-specific cytotoxic cells in teleost","volume":"112","author":"Niu","year":"2020","journal-title":"Genomics"},{"key":"ref_83","doi-asserted-by":"crossref","unstructured":"Efremova, M., Vento-Tormo, M., Teichmann, S.A., and Vento-Tormo, R. (2019). CellPhoneDB v2.0: Inferring cell-cell communication from combined expression of multi-subunit receptor-ligand complexes. bioRxiv.","DOI":"10.1101\/680926"},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1038\/nbt.2859","article-title":"The dynamics and regulators of cell fate decisions are revealed by pseudotemporal ordering of single cells","volume":"32","author":"Trapnell","year":"2014","journal-title":"Nat. Biotechnol."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"979","DOI":"10.1038\/nmeth.4402","article-title":"Reversed graph embedding resolves complex single-cell trajectories","volume":"14","author":"Qiu","year":"2017","journal-title":"Nat. Methods"},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1038\/nmeth.4150","article-title":"Single-cell mRNA quantification and differential analysis with Census","volume":"14","author":"Qiu","year":"2017","journal-title":"Nat. Methods"},{"key":"ref_87","doi-asserted-by":"crossref","unstructured":"Langfelder, P., and Horvath, S. (2008). WGCNA: An R package for weighted correlation network analysis. BMC Bioinform., 9.","DOI":"10.1186\/1471-2105-9-559"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/2\/1023\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T13:03:05Z","timestamp":1768914185000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/2\/1023"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,20]]},"references-count":87,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2026,1]]}},"alternative-id":["ijms27021023"],"URL":"https:\/\/doi.org\/10.3390\/ijms27021023","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,20]]}}}