{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T16:46:37Z","timestamp":1790873197432,"version":"4.1.0"},"reference-count":27,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T00:00:00Z","timestamp":1768867200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Scientific Research Fund of Zhejiang Provincial Education Department","award":["Y202456387"],"award-info":[{"award-number":["Y202456387"]}]},{"name":"Basic Scientific Research Business Expenses Project of China Jiliang University","award":["2024YW89"],"award-info":[{"award-number":["2024YW89"]}]},{"name":"Basic Scientific Research Business Expenses Project of China Jiliang University","award":["2024YW110"],"award-info":[{"award-number":["2024YW110"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Rice blast, caused by the fungus Magnaporthe oryzae, is a devastating disease that threatens global food security, causing annual yield losses of 10\u201330%. Consequently, novel control strategies beyond conventional fungicides are urgently needed. Tetramycin, a polyene macrolide antibiotic, is known for its broad-spectrum antifungal activity. However, the specific mechanisms underlying its efficacy against rice blast remain to be fully elucidated. In this study, we demonstrate that tetramycin confers resistance through a dual mode of action. First, in vitro assays revealed that tetramycin directly inhibits M. oryzae mycelial growth. Second, and more critically, it functions as a potent immune elicitor in Oryza sativa. Transcriptome analysis coupled with physiological assays showed that tetramycin treatment triggers a rapid oxidative burst, characterized by significantly elevated activities of key defense enzymes, including superoxide dismutase, peroxidase, phenylalanine ammonia lyase, and polyphenol oxidase (PPO). This oxidative response is further orchestrated through the simultaneous activation of the jasmonic acid (JA) and salicylic acid (SA) signaling pathways, as evidenced by the distinct upregulation of their respective biosynthetic genes and hormone levels. Collectively, these findings indicate that tetramycin not only acts as a direct fungicide but also primes the rice innate immune system via a synergistic reactive oxygen species-JA-SA signaling network, offering a sustainable strategy for rice blast management.<\/jats:p>","DOI":"10.3390\/ijms27021024","type":"journal-article","created":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T13:02:07Z","timestamp":1768914127000},"page":"1024","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Mechanisms of Tetramycin-Induced Resistance to Rice Blast Disease in Oryza sativa L."],"prefix":"10.3390","volume":"27","author":[{"given":"Hui","family":"Jiang","sequence":"first","affiliation":[{"name":"Key Laboratory of Microbiological Metrology, Measurement & Bio-Product Quality Security, State Administration for Market Regulation, College of Life Science, China Jiliang University, Hangzhou 310018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caixia","family":"Zhao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Microbiological Metrology, Measurement & Bio-Product Quality Security, State Administration for Market Regulation, College of Life Science, China Jiliang University, Hangzhou 310018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danting","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Microbiological Metrology, Measurement & Bio-Product Quality Security, State Administration for Market Regulation, College of Life Science, China Jiliang University, Hangzhou 310018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7567-7428","authenticated-orcid":false,"given":"Kai","family":"Sun","sequence":"additional","affiliation":[{"name":"Key Laboratory of Microbiological Metrology, Measurement & Bio-Product Quality Security, State Administration for Market Regulation, College of Life Science, China Jiliang University, Hangzhou 310018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yipeng","family":"Xu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Microbiological Metrology, Measurement & Bio-Product Quality Security, State Administration for Market Regulation, College of Life Science, China Jiliang University, Hangzhou 310018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1354-4926","authenticated-orcid":false,"given":"Kun","family":"Pang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Microbiological Metrology, Measurement & Bio-Product Quality Security, State Administration for Market Regulation, College of Life Science, China Jiliang University, Hangzhou 310018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoping","family":"Yu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Microbiological Metrology, Measurement & Bio-Product Quality Security, State Administration for Market Regulation, College of Life Science, China Jiliang University, Hangzhou 310018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8844-4228","authenticated-orcid":false,"given":"Xuping","family":"Shentu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Microbiological Metrology, Measurement & Bio-Product Quality Security, State Administration for Market Regulation, College of Life Science, China Jiliang University, Hangzhou 310018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,1,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Duan, S., Ai, H., Liu, S., Zhou, A., Cao, Y., and Huang, X. (2024). Functional nutritional rice: Current progresses and future prospects. Front. Plant Sci., 15.","DOI":"10.3389\/fpls.2024.1488210"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"121925","DOI":"10.1016\/j.envpol.2023.121925","article-title":"The application of zinc oxide nanoparticles: An effective strategy to protect rice from rice blast and abiotic stresses","volume":"331","author":"Qiu","year":"2023","journal-title":"Environ. Pollut."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"414","DOI":"10.1111\/j.1364-3703.2011.00783.x","article-title":"The Top 10 fungal pathogens in molecular plant pathology","volume":"13","author":"Dean","year":"2012","journal-title":"Mol. Plant Pathol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1111\/ppa.13283","article-title":"Pathogenicity and population structure analysis of Pyricularia oryzae in different districts of Jiangsu province, China","volume":"70","author":"Qi","year":"2020","journal-title":"Plant Pathol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/j.tibtech.2008.12.002","article-title":"Against the grain: Safeguarding rice from rice blast disease","volume":"27","author":"Skamnioti","year":"2009","journal-title":"Trends Biotechnol."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Devanna, B.N., Jain, P., Solanke, A.U., Das, A., Thakur, S., Singh, P.K., Kumari, M., Dubey, H., Jaswal, R., and Pawar, D. (2022). Understanding the Dynamics of Blast Resistance in Rice-Magnaporthe oryzae Interactions. J. Fungi., 8.","DOI":"10.3390\/jof8060584"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1475","DOI":"10.1007\/s10529-013-1229-z","article-title":"In vitro antifungal activity of antifungalmycin 702, a new polyene macrolide antibiotic, against the rice blast fungus Magnaporthe grisea","volume":"35","author":"Xiong","year":"2013","journal-title":"Biotechnol. Lett."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"111013","DOI":"10.1016\/j.ecoenv.2020.111013","article-title":"Mixtures of an insecticide, a fungicide and a herbicide induce high toxicities and systemic physiological disturbances in winter Apis mellifera honey bees","volume":"203","author":"Almasri","year":"2020","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1016\/j.envint.2009.10.005","article-title":"Contamination of vineyard soils with fungicides: A review of environmental and toxicological aspects","volume":"36","author":"Komarek","year":"2010","journal-title":"Environ. Int."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"12025","DOI":"10.1088\/1755-1315\/894\/1\/012025","article-title":"Pesticide residues in food and potential risk of health problems: A systematic literature review","volume":"894","author":"Yura","year":"2021","journal-title":"IOP Conf. Ser. Earth Environ. Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2234","DOI":"10.1002\/cbic.201200402","article-title":"Genome mining of the biosynthetic gene cluster of the polyene macrolide antibiotic tetramycin and characterization of a P450 monooxygenase involved in the hydroxylation of the tetramycin B polyol segment","volume":"13","author":"Cao","year":"2012","journal-title":"Chembiochem"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"602","DOI":"10.1016\/j.micres.2013.09.017","article-title":"Enhancement of nystatin production by redirecting precursor fluxes after disruption of the tetramycin gene from Streptomyces ahygroscopicus","volume":"169","author":"Ren","year":"2014","journal-title":"Microbiol. Res."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"863","DOI":"10.1094\/PDIS-09-17-1396-RE","article-title":"Baseline Sensitivity and Control Efficacy of Tetramycin Against Phytophthora capsici Isolates in China","volume":"102","author":"Ma","year":"2018","journal-title":"Plant Dis."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.pestbp.2018.02.012","article-title":"Toxicity and biochemical action of the antibiotic fungicide tetramycin on Colletotrichum scovillei","volume":"147","author":"Gao","year":"2018","journal-title":"Pestic. Biochem. Physiol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"501","DOI":"10.7164\/antibiotics.54.501","article-title":"Association of induced disease resistance of rhododendron seedlings with inoculation of Streptomyces sp. R-5 and treatment with actinomycin D and amphotericin B to the tissue-culture medium","volume":"54","author":"Shimizu","year":"2001","journal-title":"J. Antibiot."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2919","DOI":"10.1007\/s11274-012-1102-6","article-title":"Suppression of Fusarium oxysporum and induced resistance of plants involved in the biocontrol of Cucumber Fusarium Wilt by Streptomyces bikiniensis HD-087","volume":"28","author":"Zhao","year":"2012","journal-title":"World J. Microbiol. Biotechnol."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Tian, B., Tang, C., Liu, J., Jin, B., and Zhang, C. (2025). Tetramycin ameliorates tebuconazole.azoxystrobin to control leaf spot and viral diseases of Taizishen. Front. Plant Sci., 16.","DOI":"10.3389\/fpls.2025.1543462"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Jin, H., Jiang, H., Li, D., Xu, Y., Sun, K., Pang, K., Yu, X., and Shentu, X. (2025). Antifungal activity and mechanism of the antibiotic fungicide tetramycin against Magnaporthe oryzae. Front. Agron., 7.","DOI":"10.3389\/fagro.2025.1674503"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Wang, Q., Zhang, C., Long, Y., Wu, X., Su, Y., Lei, Y., and Ai, Q. (2021). Bioactivity and Control Efficacy of the Novel Antibiotic Tetramycin against Various Kiwifruit Diseases. Antibiotics, 10.","DOI":"10.3390\/antibiotics10030289"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Dow, L., Gallart, M., Ramarajan, M., Law, S.R., and Thatcher, L.F. (2023). Streptomyces and their specialised metabolites for phytopathogen control\u2014Comparative in vitro and in planta metabolic approaches. Front. Plant Sci., 14.","DOI":"10.3389\/fpls.2023.1151912"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1038\/nature05286","article-title":"The plant immune system","volume":"444","author":"Jones","year":"2006","journal-title":"Nature"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1146\/annurev-cellbio-092910-154055","article-title":"Hormonal modulation of plant immunity","volume":"28","author":"Pieterse","year":"2012","journal-title":"Annu. Rev. Cell Dev. Biol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"E1192","DOI":"10.1073\/pnas.1201616109","article-title":"Plant hormone jasmonate prioritizes defense over growth by interfering with gibberellin signaling cascade","volume":"109","author":"Yang","year":"2012","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Fan, H., Yan, X., Fu, M., Liu, D., Awan, A.W., Chen, P., Rasheed, S.M., Gao, L., and Zhang, R. (2022). Interactive Effect of Biological Agents Chitosan, Lentinan and Ningnanmycin on Papaya Ringspot Virus Resistance in Papaya (Carica papaya L.). Molecules, 27.","DOI":"10.3390\/molecules27217474"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Ma, J., Morel, J.B., Riemann, M., and Nick, P. (2022). Jasmonic acid contributes to rice resistance against Magnaporthe oryzae. BMC Plant Biol., 22.","DOI":"10.1186\/s12870-022-03948-4"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Haghpanah, M., Namdari, A., Kaleji, M.K., Nikbakht-Dehkordi, A., Arzani, A., and Araniti, F. (2025). Interplay Between ROS and Hormones in Plant Defense Against Pathogens. Plants, 14.","DOI":"10.3390\/plants14091297"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"122159","DOI":"10.1016\/j.carbpol.2024.122159","article-title":"The quantitative molecular weight-antimicrobial acivity relationship for chitosan polymers, oligomers, and derivatives","volume":"337","year":"2024","journal-title":"Carbohydr. Polym."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/2\/1024\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T13:03:12Z","timestamp":1768914192000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/2\/1024"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,20]]},"references-count":27,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2026,1]]}},"alternative-id":["ijms27021024"],"URL":"https:\/\/doi.org\/10.3390\/ijms27021024","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,20]]}}}