{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T04:11:01Z","timestamp":1787976661405,"version":"build-2784847793"},"reference-count":55,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T00:00:00Z","timestamp":1769040000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003336","name":"Bulgarian National Science Fund","doi-asserted-by":"publisher","award":["KP-06-Austria\/10\/21.08.2023"],"award-info":[{"award-number":["KP-06-Austria\/10\/21.08.2023"]}],"id":[{"id":"10.13039\/501100003336","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Folate receptor alpha is expressed at low levels in normal tissues, but is elevated in aggressive breast cancer types and can be utilized for targeted nanoparticle delivery. Hence, we prepared a hybrid nanocarrier based on in-house synthesized mesoporous silica nanoparticles (MSNs) which were further lipid-coated and reinforced with folic acid (FA). Thorough physicochemical evaluation was performed including dynamic light scattering (DLS), powder x-ray diffraction (PXRD), thermogravimetric analysis (TGA), and nitrogen physisorption. In vitro dissolution of the model drug doxorubicin was carried out in release media with pH 7.4 and pH 5.5. The cytotoxic potential and cellular uptake were investigated in MCF-7 breast cancer cells via the MTT assay, doxorubicin fluorescence measurement, and microscopy. The potential amelioration of doxorubicin\u2019s cardiotoxicity was evaluated in vitro on the H9c2 cell line. The results showed MSNs with significant pore volume (1.38 cm3\/g) and relatively small sizes (98.05 \u00b1 1.34 nm). The lipid coat and FA attachment improved the physicochemical stability and sustained release pattern over 24 h. MSNs were non-toxic, while when doxorubicin-loaded, they caused moderate cytotoxicity. The highest cytotoxic activity was observed with folate-functionalized, doxorubicin-loaded nanoparticles (NPs). Even though non-loaded folate-functionalized NPs exhibited significant cytotoxicity, their physical mixture with doxorubicin was inferior in MCF-7 cytotoxicity as opposed to the corresponding loaded nanocarrier. Fluorescence-based quantification showed a higher intracellular accumulation of doxorubicin when delivered via NPs. These results demonstrate the potential to use folate-functionalized NPs as carriers for doxorubicin delivery in breast cancer cells. Its cardiotoxicity was significantly reduced in the case of loading onto the folic acid-functionalized lipid-coated MSNs. All these findings provide a promising proof-of-concept, although further experimental validation, particularly regarding targeting selectivity and safety, is required.<\/jats:p>","DOI":"10.3390\/ijms27021092","type":"journal-article","created":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T09:16:59Z","timestamp":1769159819000},"page":"1092","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Synthesis of Folic Acid-Functionalized Hybrid Mesoporous Silica Nanoparticles and In Vitro Evaluation on MCF-7 Breast Cancer Cells"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5335-461X","authenticated-orcid":false,"given":"Marta","family":"Slavkova","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Technology and Biopharmaceutics, Faculty of Pharmacy, Medical University of Sofia, 1000 Sofia, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1029-9870","authenticated-orcid":false,"given":"Yordan","family":"Yordanov","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Pharmacotherapy and Toxicology, Faculty of Pharmacy, Medical University of Sofia, 1000 Sofia, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6536-4898","authenticated-orcid":false,"given":"Christina","family":"Voycheva","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Technology and Biopharmaceutics, Faculty of Pharmacy, Medical University of Sofia, 1000 Sofia, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6690-6740","authenticated-orcid":false,"given":"Teodora","family":"Popova","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Technology and Biopharmaceutics, Faculty of Pharmacy, Medical University of Sofia, 1000 Sofia, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6059-9508","authenticated-orcid":false,"given":"Ivanka","family":"Spassova","sequence":"additional","affiliation":[{"name":"Institute of General and Inorganic Chemistry, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1799-0851","authenticated-orcid":false,"given":"Daniela","family":"Kovacheva","sequence":"additional","affiliation":[{"name":"Institute of General and Inorganic Chemistry, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Virginia","family":"Tzankova","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Pharmacotherapy and Toxicology, Faculty of Pharmacy, Medical University of Sofia, 1000 Sofia, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Borislav","family":"Tzankov","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Technology and Biopharmaceutics, Faculty of Pharmacy, Medical University of Sofia, 1000 Sofia, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,1,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Elmadani, M., Mokaya, P.O., Omer, A.A.A., Kiptulon, E.K., Klara, S., and Orsolya, M. (2025). Cancer Burden in Europe: A Systematic Analysis of the GLOBOCAN Database (2022). BMC Cancer, 25.","DOI":"10.1186\/s12885-025-13862-1"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1038\/s41392-024-02108-4","article-title":"Breast Cancer: Pathogenesis and Treatments","volume":"10","author":"Xiong","year":"2025","journal-title":"Signal Transduct. Target. Ther."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"15330338221141795","DOI":"10.1177\/15330338221141795","article-title":"High Expression of Folate Receptor Alpha (FOLR1) Is Associated With Aggressive Tumor Behavior, Poor Response to Chemoradiotherapy, and Worse Survival in Rectal Cancer","volume":"21","author":"Chen","year":"2022","journal-title":"Technol. Cancer Res. Treat."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1186\/s12951-016-0201-1","article-title":"Folate Receptor-Targeted Nanoparticle Delivery of HuR-RNAi Suppresses Lung Cancer Cell Proliferation and Migration","volume":"14","author":"Muralidharan","year":"2016","journal-title":"J. Nanobiotechnol."},{"key":"ref_5","first-page":"1","article-title":"Coating Methotrexate-PLGA Nanoparticles with Folic Acid-Chitosan Conjugate for Cancer Targeting","volume":"71","author":"Altawabeyeh","year":"2024","journal-title":"Pharmacia"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"109207","DOI":"10.1016\/j.carres.2024.109207","article-title":"GSH-Responsive and Folate Receptor-Targeted Pyridine Bisfolate-Encapsulated Chitosan Nanoparticles for Enhanced Intracellular Drug Delivery in MCF\u22127 Cells","volume":"543","author":"Elshami","year":"2024","journal-title":"Carbohydr. Res."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"113525","DOI":"10.1016\/j.biopha.2022.113525","article-title":"Folate Receptor-Mediated Delivery of Mitoxantrone-Loaded Solid Lipid Nanoparticles to Breast Cancer Cells","volume":"154","author":"Granja","year":"2022","journal-title":"Biomed. Pharmacother."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"100128","DOI":"10.1016\/j.nxmate.2024.100128","article-title":"Role of Nanoparticles in Enhancing Chemotherapy Efficacy for Cancer Treatment","volume":"2","author":"Ghazal","year":"2024","journal-title":"Next Mater."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1186\/s12943-023-01865-0","article-title":"Progressing Nanotechnology to Improve Targeted Cancer Treatment: Overcoming Hurdles in Its Clinical Implementation","volume":"22","author":"Chehelgerdi","year":"2023","journal-title":"Mol. Cancer"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"512","DOI":"10.1016\/j.jconrel.2022.03.043","article-title":"Strategies to Improve the EPR Effect: A Mechanistic Perspective and Clinical Translation","volume":"345","author":"Wang","year":"2022","journal-title":"J. Control. Release"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"714","DOI":"10.1038\/s44222-024-00203-3","article-title":"Nanoparticle Delivery to Tumours: From EPR and ATR Mechanisms to Clinical Impact","volume":"2","author":"Dasgupta","year":"2024","journal-title":"Nat. Rev. Bioeng."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"100299","DOI":"10.1016\/j.mtadv.2022.100299","article-title":"Fabrication of Active Targeting Lipid Nanoparticles: Challenges and Perspectives","volume":"16","author":"Menon","year":"2022","journal-title":"Mater. Today Adv."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"101332","DOI":"10.1016\/j.ijcha.2023.101332","article-title":"Doxorubicin-Induced Cardiotoxicity and Risk Factors","volume":"50","author":"Belger","year":"2024","journal-title":"IJC Heart Vasc."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1007\/s13346-024-01648-0","article-title":"A Comprehensive Review on Doxorubicin: Mechanisms, Toxicity, Clinical Trials, Combination Therapies and Nanoformulations in Breast Cancer","volume":"15","author":"Bisht","year":"2025","journal-title":"Drug Deliv. Transl. Res."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"8766","DOI":"10.1021\/acs.chemmater.7b03033","article-title":"Cancer-Targeting Ultrasmall Silica Nanoparticles for Clinical Translation: Physicochemical Structure and Biological Property Correlations","volume":"29","author":"Chen","year":"2017","journal-title":"Chem. Mater."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Kashapov, R., Ibragimova, A., Pavlov, R., Gabdrakhmanov, D., Kashapova, N., Burilova, E., Zakharova, L., and Sinyashin, O. (2021). Nanocarriers for Biomedicine: From Lipid Formulations to Inorganic and Hybrid Nanoparticles. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22137055"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Amin, M.U., Ali, S., Tariq, I., Ali, M.Y., Pinnapreddy, S.R., Preis, E., W\u00f6lk, C., Harvey, R.D., Hause, G., and Br\u00fc\u00dfler, J. (2021). Ultrasound-Responsive Smart Drug Delivery System of Lipid Coated Mesoporous Silica Nanoparticles. Pharmaceutics, 13.","DOI":"10.3390\/pharmaceutics13091396"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1016\/j.actbio.2020.07.027","article-title":"Engineering of Monosized Lipid-Coated Mesoporous Silica Nanoparticles for CRISPR Delivery","volume":"114","author":"Noureddine","year":"2020","journal-title":"Acta Biomater."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"5097","DOI":"10.1021\/acsbiomaterials.3c00989","article-title":"Correction to \u201cLipid-Coated MCM-41 Mesoporous Silica Nanoparticles Loaded with Berberine Improved Inhibition of Acetylcholine Esterase and Amyloid Formation\u201d","volume":"9","author":"Singh","year":"2023","journal-title":"ACS Biomater. Sci. Eng."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Luchini, A., and Vitiello, G. (2019). Understanding the Nano-Bio Interfaces: Lipid-Coatings for Inorganic Nanoparticles as Promising Strategy for Biomedical Applications. Front. Chem., 7.","DOI":"10.3389\/fchem.2019.00343"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"104821","DOI":"10.1016\/j.jddst.2023.104821","article-title":"Preparation and Characterization of Glyceryl Dibehenate and Glyceryl Monostearate -Based Lyotropic Liquid Crystal Nanoparticles as Carriers for Hydrophobic Drugs","volume":"87","author":"Bahadori","year":"2023","journal-title":"J. Drug Deliv. Sci. Technol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"121802","DOI":"10.1016\/j.lfs.2023.121802","article-title":"Recent Advances in Using Folate Receptor 1 (FOLR1) for Cancer Diagnosis and Treatment, with an Emphasis on Cancers That Affect Women","volume":"326","author":"Varaganti","year":"2023","journal-title":"Life Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1051","DOI":"10.1515\/pac-2014-1117","article-title":"Physisorption of Gases, with Special Reference to the Evaluation of Surface Area and Pore Size Distribution (IUPAC Technical Report)","volume":"87","author":"Thommes","year":"2015","journal-title":"Pure Appl. Chem."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"136412","DOI":"10.1016\/j.molstruc.2023.136412","article-title":"Crystal Structure Determination and DFT Analysis of Doxorubicin Hydrochloride for Controlled-Release Drug Formulations","volume":"1294","author":"Bezzon","year":"2023","journal-title":"J. Mol. Struct."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"386","DOI":"10.1007\/s11483-020-09633-z","article-title":"Influence of Soya Lecithin, Sorbitan and Glyceryl Monostearate on Physicochemical Properties of Organogels","volume":"15","author":"Ghan","year":"2020","journal-title":"Food Biophys."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/S0168-3659(99)00223-0","article-title":"Vitamin A-Loaded Solid Lipid Nanoparticles for Topical Use: Drug Release Properties","volume":"66","author":"Jenning","year":"2000","journal-title":"J. Control. Release"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Hosonuma, M., and Yoshimura, K. (2023). Association between pH Regulation of the Tumor Microenvironment and Immunological State. Front. Oncol., 13.","DOI":"10.3389\/fonc.2023.1175563"},{"key":"ref_28","first-page":"3966386","article-title":"Acidic Tumor Microenvironment Promotes Pancreatic Cancer through miR-451a\/MEF2D Axis","volume":"2022","author":"Xu","year":"2022","journal-title":"J. Oncol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"237","DOI":"10.2174\/1389200218666170105165444","article-title":"Reduction of Doxorubicin-Induced Cardiotoxicity Using Nanocarriers: A Review","volume":"18","author":"Fojtu","year":"2017","journal-title":"Curr. Drug Metab."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"115836","DOI":"10.1016\/j.biopha.2023.115836","article-title":"PEG-Modified Carbon-Based Nanoparticles as Tumor-Targeted Drug Delivery System Reducing Doxorubicin-Induced Cardiotoxicity","volume":"168","author":"Wang","year":"2023","journal-title":"Biomed. Pharmacother."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1209","DOI":"10.1016\/j.biopha.2017.09.059","article-title":"A Review on the Efficacy and Toxicity of Different Doxorubicin Nanoparticles for Targeted Therapy in Metastatic Breast Cancer","volume":"95","author":"Shafei","year":"2017","journal-title":"Biomed. Pharmacother."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1016\/j.micromeso.2015.12.024","article-title":"Controlled Synthesis of Monodispersed Mesoporous Silica Nanoparticles: Particle Size Tuning and Formation Mechanism Investigation","volume":"225","author":"Lv","year":"2016","journal-title":"Microporous Mesoporous Mater."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Kim, M.-K., Ki, D.-H., Na, Y.-G., Lee, H.-S., Baek, J.-S., Lee, J.-Y., Lee, H.-K., and Cho, C.-W. (2021). Optimization of Mesoporous Silica Nanoparticles through Statistical Design of Experiment and the Application for the Anticancer Drug. Pharmaceutics, 13.","DOI":"10.3390\/pharmaceutics13020184"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"26880","DOI":"10.1021\/acsami.5b09483","article-title":"Enhancing Cellular Uptake and Doxorubicin Delivery of Mesoporous Silica Nanoparticles via Surface Functionalization: Effects of Serum","volume":"7","author":"Shahabi","year":"2015","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"13379","DOI":"10.1021\/acs.langmuir.1c02056","article-title":"Zeta Potential and Colloidal Stability Predictions for Inorganic Nanoparticle Dispersions: Effects of Experimental Conditions and Electrokinetic Models on the Interpretation of Results","volume":"37","author":"Pochapski","year":"2021","journal-title":"Langmuir"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1865","DOI":"10.1517\/17425247.2014.935335","article-title":"Compritol 888 ATO: A Multifunctional Lipid Excipient in Drug Delivery Systems and Nanopharmaceuticals","volume":"11","author":"Aburahma","year":"2014","journal-title":"Expert. Opin. Drug Deliv."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"834","DOI":"10.1007\/s44174-023-00153-1","article-title":"Multifaceted Applications of Solid Lipid: A Comprehensive Review","volume":"2","author":"Patra","year":"2024","journal-title":"Biomed. Mater. Devices"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"95","DOI":"10.3390\/ddc2010006","article-title":"Stearylamine Liposome as an Anti-Parasitic Agent","volume":"2","author":"Vassoudevane","year":"2023","journal-title":"Drugs Drug Candidates"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.ejpb.2016.10.013","article-title":"Lipid Nanoparticles (SLN, NLC): Overcoming the Anatomical and Physiological Barriers of the Eye\u2013Part II-Ocular Drug-Loaded Lipid Nanoparticles","volume":"110","author":"Espina","year":"2017","journal-title":"Eur. J. Pharm. Biopharm."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"33235","DOI":"10.1021\/acsomega.0c04925","article-title":"Dimerization of Doxorubicin Causes Its Precipitation","volume":"5","author":"Yamada","year":"2020","journal-title":"ACS Omega"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"110543","DOI":"10.1016\/j.micromeso.2020.110543","article-title":"Aminated Hollow Mesoporous Silica Nanoparticles as an Enhanced Loading and Sustained Releasing Carrier for Doxorubicin Delivery","volume":"309","author":"Nguyen","year":"2020","journal-title":"Microporous Mesoporous Mater."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"524","DOI":"10.1177\/0885328217724638","article-title":"Amino-Functionalized Mesoporous Silica Nanoparticles as Efficient Carriers for Anticancer Drug Delivery","volume":"32","author":"He","year":"2017","journal-title":"J. Biomater. Appl."},{"key":"ref_43","unstructured":"Grumezescu, A.M. (2019). Chapter 5-Reversible Core\u2013Shell Crosslinked Micelles for Controlled Release of Bioactive Agents. Nanoarchitectonics in Biomedicine, William Andrew Publishing."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1007\/s00280-022-04400-y","article-title":"Cytotoxic Mechanisms of Doxorubicin at Clinically Relevant Concentrations in Breast Cancer Cells","volume":"89","author":"Nicoletto","year":"2022","journal-title":"Cancer Chemother. Pharmacol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1038\/s41427-021-00320-0","article-title":"Simulation of Nanoparticles Interacting with a Cell Membrane: Probing the Structural Basis and Potential Biomedical Application","volume":"13","author":"Zhang","year":"2021","journal-title":"NPG Asia Mater."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"103800","DOI":"10.1016\/j.jddst.2022.103800","article-title":"pH-Responsive Sustained Delivery of Doxorubicin Using Aminated and PEGylated Mesoporous Silica Nanoparticles Leads to Enhanced Antitumor Efficacy in Pre-Clinical Orthotopic Breast Cancer Model","volume":"77","author":"Mal","year":"2022","journal-title":"J. Drug Deliv. Sci. Technol."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Day, C.M., Sweetman, M.J., Song, Y., Plush, S.E., and Garg, S. (2021). Functionalized Mesoporous Silica Nanoparticles as Delivery Systems for Doxorubicin: Drug Loading and Release. Appl. Sci., 11.","DOI":"10.3390\/app11136121"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Bayoumi, M., Youshia, J., El-Kawy, O.A., Gaber, S.A.A., Arafa, M.G., Nasr, M., and Sammour, O.A. (2025). Folic Acid-Decorated Lipidic Nanocapsules Co-Loaded with Atorvastatin and Curcumin to Enhance Glioma Targeting in Mice. Pharmaceuticals, 18.","DOI":"10.3390\/ph18111623"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1124\/mol.119.115725","article-title":"Insights into Doxorubicin-Induced Cardiotoxicity: Molecular Mechanisms, Preventive Strategies, and Early Monitoring","volume":"96","author":"Wenningmann","year":"2019","journal-title":"Mol. Pharmacol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"778","DOI":"10.1016\/j.ijpharm.2012.07.061","article-title":"Carboxylic Modified Spherical Mesoporous Silicas As Drug Delivery Carriers","volume":"436","author":"Popova","year":"2012","journal-title":"Int. J. Pharm."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.bios.2018.05.025","article-title":"Probing the Specific Binding of Folic Acid to Folate Receptor Using Amino-Functionalized Mesoporous Silica Nanoparticles for Differentiation of MCF 7 Tumoral Cells from MCF 10A","volume":"115","author":"Soleymani","year":"2018","journal-title":"Biosens. Bioelectron."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1080\/10837450.2019.1703739","article-title":"Folic Acid Receptor-Targeted Solid Lipid Nanoparticles to Enhance Cytotoxicity of Letrozole through Induction of Caspase-3 Dependent-Apoptosis for Breast Cancer Treatment","volume":"25","author":"Yassemi","year":"2020","journal-title":"Pharm. Dev. Technol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/0022-1759(83)90303-4","article-title":"Rapid Colorimetric Assay for Cellular Growth and Survival: Application to Proliferation and Cytotoxicity Assays","volume":"65","author":"Mosmann","year":"1983","journal-title":"J. Immunol. Methods"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1038\/nmeth.2089","article-title":"NIH Image to ImageJ: 25 Years of Image Analysis","volume":"9","author":"Schneider","year":"2012","journal-title":"Nat. Methods"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"14836","DOI":"10.1038\/ncomms14836","article-title":"A BaSiC Tool for Background and Shading Correction of Optical Microscopy Images","volume":"8","author":"Peng","year":"2017","journal-title":"Nat. Commun."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/2\/1092\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T09:19:30Z","timestamp":1769159970000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/2\/1092"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,22]]},"references-count":55,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2026,1]]}},"alternative-id":["ijms27021092"],"URL":"https:\/\/doi.org\/10.3390\/ijms27021092","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,22]]}}}