{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T18:10:51Z","timestamp":1790964651007,"version":"4.1.0"},"reference-count":39,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T00:00:00Z","timestamp":1769040000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Universidad Nacional de San Luis (UNSL), Argentina","award":["PROICO 02-0720"],"award-info":[{"award-number":["PROICO 02-0720"]}]},{"name":"Institutional Research Program","award":["238\/21"],"award-info":[{"award-number":["238\/21"]}]},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Argentina","award":["PIP 2021-2023\/00969"],"award-info":[{"award-number":["PIP 2021-2023\/00969"]}]},{"DOI":"10.13039\/501100003074","name":"Agencia Nacional de Promoci\u00f3n Cient\u00edfica y Tecnol\u00f3gica (ANPCyT), Argentina","doi-asserted-by":"crossref","award":["PICT-2020-SERIEA-03793"],"award-info":[{"award-number":["PICT-2020-SERIEA-03793"]}],"id":[{"id":"10.13039\/501100003074","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Endometriosis (EDT) is a chronic, estrogen-dependent disease characterized by inflammation, fibrosis, pelvic pain, and infertility. Current therapies show limited long-term efficacy and adverse effects, underscoring the need for novel therapeutic approaches. Elevated copper (Cu) levels have been reported in both patients and animal models of EDT, making Cu chelation a promising strategy. This work aimed to evaluate the impact of ammonium tetrathiomolybdate (TM) on the expression of markers related to the interconnected processes of inflammation, innervation, and fibrogenesis in mice with induced EDT. Twenty-four female C57BL\/6 mice were assigned to Sham, EDT, or EDT+TM groups. Treatment with TM began on postoperative day 15, with samples collected one month after EDT induction. Peritoneal fluid cytokines (TNF-\u03b1, IL-1\u03b2, IL-6, TGF-\u03b21) were quantified by ELISA. Endometriotic-like lesions were examined for mRNA expression of cytokines, neurotrophins (Ngf, Bdnf, Ngfr), neural markers (Uchl1, Gap43), neuropeptides and nociceptive markers (Tac1\/Tacr1, Calca\/Calcrl\/Ramp1, Trpv1), and fibrogenic markers (Vim, Acta2, Col1a1, Fmod) by RT-qPCR. Neurotrophin protein levels were measured by ELISA, and collagen content was assessed through Masson\u2019s staining. TM significantly modulated inflammatory, neural, nociceptive, and fibrogenic markers, reducing most of them along with collagen content. These findings suggest that TM could impact key pathological mechanisms involved in EDT.<\/jats:p>","DOI":"10.3390\/ijms27021099","type":"journal-article","created":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T09:16:59Z","timestamp":1769159819000},"page":"1099","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Copper-Targeted Therapy in Experimental Endometriosis: Effects of Ammonium Tetrathiomolybdate on Markers of the Interconnected Processes of Inflammation, Innervation, and Fibrogenesis"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2556-1205","authenticated-orcid":false,"given":"Mar\u00eda Bel\u00e9n","family":"Delsouc","sequence":"first","affiliation":[{"name":"Laboratorio de Biolog\u00eda de la Reproducci\u00f3n, Instituto Multidisciplinario de Investigaciones Biol\u00f3gicas de San Luis (IMIBIO-SL), CONICET, Facultad de Qu\u00edmica, Bioqu\u00edmica y Farmacia, Universidad Nacional de San Luis (UNSL), San Luis D5700HHW, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3434-2178","authenticated-orcid":false,"given":"Roc\u00edo Ayelem","family":"Conforti","sequence":"additional","affiliation":[{"name":"Laboratorio de Biolog\u00eda de la Reproducci\u00f3n, Instituto Multidisciplinario de Investigaciones Biol\u00f3gicas de San Luis (IMIBIO-SL), CONICET, Facultad de Qu\u00edmica, Bioqu\u00edmica y Farmacia, Universidad Nacional de San Luis (UNSL), San Luis D5700HHW, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-2488-0978","authenticated-orcid":false,"given":"Ana Sofia","family":"Zabala","sequence":"additional","affiliation":[{"name":"Laboratorio de Biolog\u00eda de la Reproducci\u00f3n, Instituto Multidisciplinario de Investigaciones Biol\u00f3gicas de San Luis (IMIBIO-SL), CONICET, Facultad de Qu\u00edmica, Bioqu\u00edmica y Farmacia, Universidad Nacional de San Luis (UNSL), San Luis D5700HHW, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2039-0731","authenticated-orcid":false,"given":"Ver\u00f3nica Palmira","family":"Filippa","sequence":"additional","affiliation":[{"name":"Laboratorio de Histolog\u00eda, Facultad de Qu\u00edmica, Bioqu\u00edmica y Farmacia, Universidad Nacional de San Luis (UNSL), San Luis D5700HHW, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0598-4101","authenticated-orcid":false,"given":"Leonardo","family":"Mari\u00f1o-Repizo","sequence":"additional","affiliation":[{"name":"Instituto de Qu\u00edmica de San Luis (INQUISAL), CONICET, San Luis D5700HHW, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7031-0643","authenticated-orcid":false,"given":"Sandra Silvina","family":"Vallcaneras","sequence":"additional","affiliation":[{"name":"Laboratorio de Biolog\u00eda de la Reproducci\u00f3n, Instituto Multidisciplinario de Investigaciones Biol\u00f3gicas de San Luis (IMIBIO-SL), CONICET, Facultad de Qu\u00edmica, Bioqu\u00edmica y Farmacia, Universidad Nacional de San Luis (UNSL), San Luis D5700HHW, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4334-5845","authenticated-orcid":false,"given":"Marilina","family":"Casais","sequence":"additional","affiliation":[{"name":"Laboratorio de Biolog\u00eda de la Reproducci\u00f3n, Instituto Multidisciplinario de Investigaciones Biol\u00f3gicas de San Luis (IMIBIO-SL), CONICET, Facultad de Qu\u00edmica, Bioqu\u00edmica y Farmacia, Universidad Nacional de San Luis (UNSL), San Luis D5700HHW, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,1,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1001\/jama.2025.2975","article-title":"Endometriosis","volume":"334","author":"Mackenzie","year":"2025","journal-title":"JAMA"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Garvey, M. 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