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Androgen deprivation therapy (ADT) has been widely used as the first-line treatment for PCa. However, most PCa will progress to castration-resistant PCa (CRPC) that resists ADT 1 to 3 years after the treatment. Steroidogenesis from cholesterol is one of the mechanisms leading to ADT resistance. In PCa cells, low-density lipoprotein (LDL) mediated uptake is the major venue to acquire cholesterol. However, the mechanism of regulating this process is not fully understood. Fibroblast growth factor receptor 1 (FGFR1) is a receptor tyrosine kinase (RTK) that is ectopically expressed in PCa cells and promotes PCa progression by activating downstream signaling pathways. To comprehensively determine the roles of FGFR1 in PCa, we generated FGFR1-null DU145 cells and compared the transcriptomes of FGFR1-null and wild-type cells. We found that ablation of FGFR1 reduced the expression of genes promoting LDL uptake and de novo synthesis of cholesterol, thereby reducing the overall cholesterol pool in PCa cells. Detailed mechanistic studies further revealed that FGFR1 boosted the activation of sterol regulatory element-binding protein 2 (SREBP2) through ERK-dependent phosphorylation and cleavage, which, in turn, increased the expression of low-density lipoprotein receptor (LDLR) and enzymes involved in de novo cholesterol synthesis. Furthermore, in silico analyses demonstrated that high expression of FGFR1 was associated with high LDLR expression and clinicopathological features in PCa. Collectively, our data unveiled a previously unrecognized therapeutic avenue for CRPC by targeting FGFR1-driven cholesterol uptake and de novo synthesis.<\/jats:p>","DOI":"10.3390\/ijms27031190","type":"journal-article","created":{"date-parts":[[2026,1,26]],"date-time":"2026-01-26T08:14:23Z","timestamp":1769415263000},"page":"1190","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Ectopic FGFR1 Increases Intracellular Pool of Cholesterol in Prostate Cancer Cells"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2012-5148","authenticated-orcid":false,"given":"Ziying","family":"Liu","sequence":"first","affiliation":[{"name":"Center for Translational Cancer Research, Institute of Biosciences and Technology, Texas A&M Health Science Center, Texas A&M University, Houston, TX 77030, USA"},{"name":"Department of Translational Medical Sciences, Naresh K. Vashisht College of Medicine, Texas A&M University, Houston, TX 77030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuepeng","family":"Ke","sequence":"additional","affiliation":[{"name":"Center for Translational Cancer Research, Institute of Biosciences and Technology, Texas A&M Health Science Center, Texas A&M University, Houston, TX 77030, USA"},{"name":"Department of Translational Medical Sciences, Naresh K. Vashisht College of Medicine, Texas A&M University, Houston, TX 77030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingting","family":"Hong","sequence":"additional","affiliation":[{"name":"Department of Translational Medical Sciences, Naresh K. Vashisht College of Medicine, Texas A&M University, Houston, TX 77030, USA"},{"name":"Center for Epigenetics and Disease Prevention, Institute of Biosciences and Technology, Texas A&M University, Houston, TX 77030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-6923-555X","authenticated-orcid":false,"given":"Kennedy","family":"Smith","sequence":"additional","affiliation":[{"name":"Center for Translational Cancer Research, Institute of Biosciences and Technology, Texas A&M Health Science Center, Texas A&M University, Houston, TX 77030, USA"},{"name":"Department of Translational Medical Sciences, Naresh K. Vashisht College of Medicine, Texas A&M University, Houston, TX 77030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Davies","sequence":"additional","affiliation":[{"name":"Center for Translational Cancer Research, Institute of Biosciences and Technology, Texas A&M Health Science Center, Texas A&M University, Houston, TX 77030, USA"},{"name":"Department of Translational Medical Sciences, Naresh K. Vashisht College of Medicine, Texas A&M University, Houston, TX 77030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Translational Medical Sciences, Naresh K. Vashisht College of Medicine, Texas A&M University, Houston, TX 77030, USA"},{"name":"Center for Epigenetics and Disease Prevention, Institute of Biosciences and Technology, Texas A&M University, Houston, TX 77030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6815-8571","authenticated-orcid":false,"given":"Dekai","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Translational Medical Sciences, Naresh K. Vashisht College of Medicine, Texas A&M University, Houston, TX 77030, USA"},{"name":"Center for Infectious and Inflammatory Diseases, Institute of Biosciences and Technology, Texas A&M University, Houston, TX 77030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4869-3198","authenticated-orcid":false,"given":"Sanjukta","family":"Chakraborty","sequence":"additional","affiliation":[{"name":"Center for Translational Cancer Research, Institute of Biosciences and Technology, Texas A&M Health Science Center, Texas A&M University, Houston, TX 77030, USA"},{"name":"Department of Medical Physiology, Naresh K. Vashisht College of Medicine, Texas A&M University, Bryan, TX 77807, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7962-0517","authenticated-orcid":false,"given":"Yubin","family":"Zhou","sequence":"additional","affiliation":[{"name":"Center for Translational Cancer Research, Institute of Biosciences and Technology, Texas A&M Health Science Center, Texas A&M University, Houston, TX 77030, USA"},{"name":"Department of Translational Medical Sciences, Naresh K. Vashisht College of Medicine, Texas A&M University, Houston, TX 77030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7800-9222","authenticated-orcid":false,"given":"Fen","family":"Wang","sequence":"additional","affiliation":[{"name":"Center for Translational Cancer Research, Institute of Biosciences and Technology, Texas A&M Health Science Center, Texas A&M University, Houston, TX 77030, USA"},{"name":"Department of Translational Medical Sciences, Naresh K. Vashisht College of Medicine, Texas A&M University, Houston, TX 77030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,1,24]]},"reference":[{"key":"ref_1","first-page":"10","article-title":"Cancer statistics, 2025","volume":"75","author":"Siegel","year":"2025","journal-title":"CA Cancer J. Clin."},{"key":"ref_2","first-page":"365","article-title":"Mechanisms of resistance in castration-resistant prostate cancer (CRPC)","volume":"4","author":"Chandrasekar","year":"2015","journal-title":"Transl. Androl. Urol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"e188127","DOI":"10.1172\/JCI188127","article-title":"Nonvesicular cholesterol transport in physiology","volume":"135","author":"Ferrari","year":"2025","journal-title":"J. Clin. Investig."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1038\/s41580-019-0190-7","article-title":"Mechanisms and regulation of cholesterol homeostasis","volume":"21","author":"Luo","year":"2020","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"474","DOI":"10.1016\/j.ccell.2017.09.003","article-title":"Androgen Receptor Pathway-Independent Prostate Cancer Is Sustained through FGF Signaling","volume":"32","author":"Bluemn","year":"2017","journal-title":"Cancer Cell"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Wang, C., Liu, Z., Ke, Y., and Wang, F. (2019). Intrinsic FGFR2 and Ectopic FGFR1 Signaling in the Prostate and Prostate Cancer. Front. Genet., 10.","DOI":"10.3389\/fgene.2019.00012"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1362","DOI":"10.1038\/bjc.2011.367","article-title":"Upregulated FGFR1 expression is associated with the transition of hormone-naive to castrate-resistant prostate cancer","volume":"105","author":"Armstrong","year":"2011","journal-title":"Br. J. Cancer"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/pros.10311","article-title":"Chronic activity of ectopic type 1 fibroblast growth factor receptor tyrosine kinase in prostate epithelium results in hyperplasia accompanied by intraepithelial neoplasia","volume":"58","author":"Wang","year":"2004","journal-title":"Prostate"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"4897","DOI":"10.1038\/sj.onc.1210288","article-title":"Conditional activation of FGFR1 in the prostate epithelium induces angiogenesis with concomitant differential regulation of Ang-1 and Ang-2","volume":"26","author":"Winter","year":"2007","journal-title":"Oncogene"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1016\/j.ccr.2007.11.004","article-title":"Inducible FGFR-1 activation leads to irreversible prostate adenocarcinoma and an epithelial-to-mesenchymal transition","volume":"12","author":"Acevedo","year":"2007","journal-title":"Cancer Cell"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"3716","DOI":"10.1158\/0008-5472.CAN-12-3274","article-title":"FGFR1 Is Essential for Prostate Cancer Progression and Metastasis","volume":"73","author":"Yang","year":"2013","journal-title":"Cancer Res."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/j.canlet.2021.11.030","article-title":"FGFR blockade by pemigatinib treats naive and castration resistant prostate cancer","volume":"526","author":"Chiodelli","year":"2022","journal-title":"Cancer Lett."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1038\/s41467-021-27322-4","article-title":"Single-cell analysis of human primary prostate cancer reveals the heterogeneity of tumor-associated epithelial cell states","volume":"13","author":"Song","year":"2022","journal-title":"Nat. Commun."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1002\/path.5751","article-title":"Single-cell analysis of mouse and human prostate reveals novel fibroblasts with specialized distribution and microenvironment interactions","volume":"255","author":"Joseph","year":"2021","journal-title":"J. Pathol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"e20210564","DOI":"10.1084\/jem.20210564","article-title":"Lipid-loaded tumor-associated macrophages sustain tumor growth and invasiveness in prostate cancer","volume":"219","author":"Masetti","year":"2022","journal-title":"J. Exp. Med."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1038\/s41467-023-36325-2","article-title":"Dissecting the immune suppressive human prostate tumor microenvironment via integrated single-cell and spatial transcriptomic analyses","volume":"14","author":"Hirz","year":"2023","journal-title":"Nat. Commun."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1180","DOI":"10.1126\/science.abn0478","article-title":"Lineage plasticity in prostate cancer depends on JAK\/STAT inflammatory signaling","volume":"377","author":"Chan","year":"2022","journal-title":"Science"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1289","DOI":"10.1038\/s41592-019-0619-0","article-title":"Fast, sensitive and accurate integration of single-cell data with Harmony","volume":"16","author":"Korsunsky","year":"2019","journal-title":"Nat. Methods"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"778","DOI":"10.1038\/s42003-020-01476-1","article-title":"Single-cell analysis supports a luminal-neuroendocrine transdifferentiation in human prostate cancer","volume":"3","author":"Dong","year":"2020","journal-title":"Commun. Biol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1088","DOI":"10.1038\/s41467-021-21246-9","article-title":"Inference and analysis of cell-cell communication using CellChat","volume":"12","author":"Jin","year":"2021","journal-title":"Nat. Commun."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"4459","DOI":"10.1158\/0008-5472.CAN-17-3226","article-title":"Aberrant FGFR Tyrosine Kinase Signaling Enhances the Warburg Effect by Reprogramming LDH Isoform Expression and Activity in Prostate Cancer","volume":"78","author":"Liu","year":"2018","journal-title":"Cancer Res."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1146\/annurev-physiol-012110-142233","article-title":"Niemann-pick C1-like 1 (NPC1L1) protein in intestinal and hepatic cholesterol transport","volume":"73","author":"Jia","year":"2011","journal-title":"Annu. Rev. Physiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1087","DOI":"10.1126\/science.aao6575","article-title":"A LIMA1 variant promotes low plasma LDL cholesterol and decreases intestinal cholesterol absorption","volume":"360","author":"Zhang","year":"2018","journal-title":"Science"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2419","DOI":"10.1038\/s41467-018-04724-5","article-title":"Spatial maps of prostate cancer transcriptomes reveal an unexplored landscape of heterogeneity","volume":"9","author":"Berglund","year":"2018","journal-title":"Nat. Commun."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"17801","DOI":"10.1074\/jbc.273.28.17801","article-title":"Second-site cleavage in sterol regulatory element-binding protein occurs at transmembrane junction as determined by cysteine panning","volume":"273","author":"Duncan","year":"1998","journal-title":"J. Biol. Chem."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"15224","DOI":"10.1074\/jbc.M800910200","article-title":"Growth factor-induced phosphorylation of sterol regulatory element-binding proteins inhibits sumoylation, thereby stimulating the expression of their target genes, low density lipoprotein uptake, and lipid synthesis","volume":"283","author":"Arito","year":"2008","journal-title":"J. Biol. Chem."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"903","DOI":"10.1016\/j.chembiol.2014.05.013","article-title":"Spatiotemporal control of fibroblast growth factor receptor signals by blue light","volume":"21","author":"Kim","year":"2014","journal-title":"Chem. Biol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1016\/j.cmet.2005.04.010","article-title":"Control of lipid metabolism by phosphorylation-dependent degradation of the SREBP family of transcription factors by SCF(Fbw7)","volume":"1","author":"Sundqvist","year":"2005","journal-title":"Cell Metab."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"R255","DOI":"10.1530\/ERC-19-0472","article-title":"Fibroblast growth factors signaling in bone metastasis","volume":"27","author":"Labanca","year":"2020","journal-title":"Endocr. Relat. Cancer"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"720","DOI":"10.3389\/fcell.2020.00720","article-title":"Editorial: Resident and Ectopic FGF Signaling in Development and Disease","volume":"8","author":"Wang","year":"2020","journal-title":"Front. Cell Dev. Biol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1011","DOI":"10.1038\/s42003-024-06704-6","article-title":"FGFR1 governs iron homeostasis via regulating intracellular protein degradation pathways of IRP2 in prostate cancer cells","volume":"7","author":"Lin","year":"2024","journal-title":"Commun. Biol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1525","DOI":"10.1002\/ijc.33922","article-title":"Crosstalk of FGFR1 signaling and choline metabolism promotes cell proliferation and survival in prostate cancer cells","volume":"150","author":"Fan","year":"2022","journal-title":"Int. J. Cancer"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"14839","DOI":"10.1074\/jbc.RA118.002907","article-title":"Ectopic fibroblast growth factor receptor 1 promotes inflammation by promoting nuclear factor-kappaB signaling in prostate cancer cells","volume":"293","author":"Wang","year":"2018","journal-title":"J. Biol. Chem."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"876","DOI":"10.1158\/2159-8290.CD-12-0400","article-title":"Compensatory pathways in oncogenic kinase signaling and resistance to targeted therapies: Six degrees of separation","volume":"2","author":"Trusolino","year":"2012","journal-title":"Cancer Discov."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"580","DOI":"10.4161\/cc.8.4.7657","article-title":"Paths of FGFR-driven tumorigenesis","volume":"8","author":"Acevedo","year":"2009","journal-title":"Cell Cycle"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1038\/s42255-020-0174-0","article-title":"Cholesterol metabolism in cancer: Mechanisms and therapeutic opportunities","volume":"2","author":"Huang","year":"2020","journal-title":"Nat. Metab."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"e0434","DOI":"10.1097\/HC9.0000000000000434","article-title":"An overview of the cholesterol metabolism and its proinflammatory role in the development of MASLD","volume":"8","author":"Zhang","year":"2024","journal-title":"Hepatol. Commun."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"10079","DOI":"10.1073\/pnas.1611956113","article-title":"Clues to the mechanism of cholesterol transfer from the structure of NPC1 middle lumenal domain bound to NPC2","volume":"113","author":"Li","year":"2016","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"6316","DOI":"10.1074\/jbc.M109.073601","article-title":"Inhibition of ERK1\/2 and activation of liver X receptor synergistically induce macrophage ABCA1 expression and cholesterol efflux","volume":"285","author":"Zhou","year":"2010","journal-title":"J. Biol. Chem."},{"key":"ref_40","first-page":"316","article-title":"Current understanding of resistance to abiraterone and enzalutamide in advanced prostate cancer","volume":"14","author":"Antonarakis","year":"2016","journal-title":"Clin. Adv. Hematol. Oncol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"3063","DOI":"10.1158\/0008-5472.CAN-18-3295","article-title":"Cholesterol Induces Epithelial-to-Mesenchymal Transition of Prostate Cancer Cells by Suppressing Degradation of EGFR through APMAP","volume":"79","author":"Jiang","year":"2019","journal-title":"Cancer Res."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1007\/s00249-014-0953-1","article-title":"Acid-mediated Lipinski\u2019s second rule: Application to drug design and targeting in cancer","volume":"43","author":"Omran","year":"2014","journal-title":"Eur. Biophys. J."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Cicione, A., Brassetti, A., Lombardo, R., Franco, A., Turchi, B., D\u2019annunzio, S., Nacchia, A., Tubaro, A., Simone, G., and De Nunzio, C. (2022). Metabolic Syndrome and Physical Inactivity May Be Shared Etiological Agents of Prostate Cancer and Coronary Heart Diseases. Cancers, 14.","DOI":"10.3390\/cancers14040936"},{"key":"ref_44","first-page":"631","article-title":"Impairment of autophagy may represent the molecular mechanism behind the relationship between obesity and inflammation in patients with BPH and LUTS","volume":"73","author":"Giglio","year":"2021","journal-title":"Minerva Urol. Nephrol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"562","DOI":"10.1038\/nprot.2012.016","article-title":"Differential gene and transcript expression analysis of RNA-seq experiments with TopHat and Cufflinks","volume":"7","author":"Trapnell","year":"2012","journal-title":"Nat. Protoc."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/3\/1190\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T05:10:42Z","timestamp":1770181842000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/3\/1190"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,24]]},"references-count":45,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2026,2]]}},"alternative-id":["ijms27031190"],"URL":"https:\/\/doi.org\/10.3390\/ijms27031190","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,24]]}}}