{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T00:38:47Z","timestamp":1789087127083,"version":"build-2803163510"},"reference-count":37,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T00:00:00Z","timestamp":1769472000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Multidrug-resistant (MDR) Escherichia coli (E. coli) at the poultry\u2013human interface motivates evaluating strictly lytic bacteriophages as targeted biocontrol candidates. A lytic E. coli phage (GADS24) was isolated from poultry waste in Saudi Arabia. Plaque formation and host range were assessed against 10 clinical E. coli isolates. Virion morphology was examined by transmission electron microscopy (TEM). Whole-genome sequencing (Illumina) and annotation (Prokka\/RAST) were followed by comparative genomics (BLASTn 2.15.0, ANI JSpeciesWS: 2014\u20132025 Ribocon GmbH\u2014Version: 5.0.3, dDDH GGDC: GGDC 3.0 and phylogenetic\/proteomic analyses for taxonomic placement. GADS24 formed clear plaques and lysed 5 of 10 clinical E. coli isolates tested. TEM revealed an icosahedral capsid (~72.6 nm) and a contractile tail (~131.7 nm), consistent with Tevenvirinae\/Mosigvirus morphology. The dsDNA genome is 168,896 bp (GC 43.8%) with 268 predicted ORFs and two tRNA genes (tRNA-Arg and tRNA-Met); no lysogeny-related genes were detected. The closest relative was Escherichia phage JN02 (98.44% ANI; 57.8% dDDH), supporting assignment to Mosigvirus while indicating a genome-resolved distinct lineage. The genome is available in GenBank (OQ703618). GADS24 represents a genome-resolved, strictly lytic Mosigvirus with in vitro biocontrol-relevant phenotyping against E. coli, supporting follow-up development for poultry-associated infection control and deeper phage\u2013host interaction studies.<\/jats:p>","DOI":"10.3390\/ijms27031276","type":"journal-article","created":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T15:41:56Z","timestamp":1769528516000},"page":"1276","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["A Lytic Mosigvirus Phage (GADS24) from a Poultry-Farm Environment: Genome-Resolved Characterization and In Vitro Biocontrol-Relevant Phenotyping Against Escherichia coli"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7803-2078","authenticated-orcid":false,"given":"Jehan","family":"Alrahimi","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, Faculty of Sciences, King Abdulaziz University, Jeddah 22254, Saudi Arabia"},{"name":"Immunology Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah 22254, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ghadah","family":"Alsubhi","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Faculty of Sciences, King Abdulaziz University, Jeddah 22254, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9599-050X","authenticated-orcid":false,"given":"Alia","family":"Aldahlawi","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Faculty of Sciences, King Abdulaziz University, Jeddah 22254, Saudi Arabia"},{"name":"Immunology Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah 22254, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5251-559X","authenticated-orcid":false,"given":"Fatemah S.","family":"Basingab","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Faculty of Sciences, King Abdulaziz University, Jeddah 22254, Saudi Arabia"},{"name":"Immunology Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah 22254, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4661-0149","authenticated-orcid":false,"given":"Mohammed A.","family":"Imam","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Parasitology, Faculty of Medicine at Al-Qunfudah, Umm Al-Qura University, Al-Qunfudah 21961, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hashim","family":"Felemban","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah 22254, Saudi Arabia"},{"name":"Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah 22254, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Najwa","family":"Alharbi","sequence":"additional","affiliation":[{"name":"Microbiology Department, Science College, King Abdulaziz University, Jeddah 22254, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sana","family":"Alshaik","sequence":"additional","affiliation":[{"name":"Microbiology Department, Science College, King Abdulaziz University, Jeddah 22254, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8355-7741","authenticated-orcid":false,"given":"Hala S.","family":"Sonbol","sequence":"additional","affiliation":[{"name":"Biochemistry Department, Faculty of Science, King Abdulaziz University, Jeddah 22254, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0909-6558","authenticated-orcid":false,"given":"Kawther","family":"Zaher","sequence":"additional","affiliation":[{"name":"Immunology Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah 22254, Saudi Arabia"},{"name":"Department of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah 22254, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1736-181X","authenticated-orcid":false,"given":"Esam I.","family":"Azhar","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah 22254, Saudi Arabia"},{"name":"Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah 22254, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,1,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1038\/nrmicro2298","article-title":"Population genetics of commensal Escherichia coli","volume":"8","author":"Tenaillon","year":"2010","journal-title":"Nat. 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