{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,31]],"date-time":"2026-01-31T01:11:22Z","timestamp":1769821882531,"version":"3.49.0"},"reference-count":70,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T00:00:00Z","timestamp":1769731200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82402580"],"award-info":[{"award-number":["82402580"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Basement membrane (BM) breaching is a critical hallmark of cutaneous squamous cell carcinoma (cSCC) invasion. This study aimed to identify novel BM-related genes (BMRGs) to effectively distinguish invasive cSCC from actinic keratosis (AK) and Bowen\u2019s disease (BD), and to identify potential therapeutic targets. Single-cell RNA sequencing was used for BMRGs identification within keratinocytes and fibroblasts clusters. Protein\u2013protein interaction network analysis and Lasso regression were performed for hub BMRGs screening, together with nomogram model construction and validation. In this study, 6\u20139 central hub BMRGs were identified for each stage during cSCC progression with a good AUC value (&gt;0.8). In keratinocytes, BMRGs such as integrins (ITGB1, ITGA3, ITGA6), laminins (LAMA3, LAMC1), CD44, and FN1 were upregulated in cSCC compared to AK or BD (adjusted p &lt; 0.05); in fibroblasts, BMRGs including ITGB1, ITGAV, LUM, BGN, SDC1, and FN1 were upregulated in cSCC (adjusted p &lt; 0.05), suggesting their collective role in BM breaching and invasion, as well as a higher risk of BD. This study provides novel biological insights into the differentiation of progression pathways from AK or BD to cSCC, as well as potential targets for therapeutic intervention.<\/jats:p>","DOI":"10.3390\/ijms27031394","type":"journal-article","created":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T13:04:46Z","timestamp":1769778286000},"page":"1394","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Identification of Basement Membrane-Related Biomarkers in the Progression of Cutaneous Squamous Cell Carcinoma"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6504-7024","authenticated-orcid":false,"given":"Shuaijun","family":"Zou","sequence":"first","affiliation":[{"name":"Department of Dermatology, Naval Medical Centre, Naval Medical University, Shanghai 200052, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sijia","family":"Huang","sequence":"additional","affiliation":[{"name":"School of Medicine, Shanghai University, Shanghai 200444, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Naval Diving Medicine, Naval Medical Centre, Naval Medical University, Shanghai 200052, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-6118-1879","authenticated-orcid":false,"given":"Ruiqian","family":"Yao","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Naval Medical Centre, Naval Medical University, Shanghai 200052, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoyan","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Naval Medical Centre, Naval Medical University, Shanghai 200052, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haixia","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Naval Medical Centre, Naval Medical University, Shanghai 200052, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lin","family":"Du","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Naval Medical Centre, Naval Medical University, Shanghai 200052, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liangzhe","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Naval Medical Centre, Naval Medical University, Shanghai 200052, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuanjie","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Naval Medical Centre, Naval Medical University, Shanghai 200052, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,1,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1038\/s41568-023-00583-5","article-title":"Advances in cutaneous squamous cell carcinoma","volume":"23","author":"Winge","year":"2023","journal-title":"Nat. Rev. Cancer"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"935","DOI":"10.1056\/NEJMoa1811850","article-title":"Randomized Trial of Four Treatment Approaches for Actinic Keratosis","volume":"380","author":"Jansen","year":"2019","journal-title":"N. Engl. J. Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1081","DOI":"10.1001\/jamadermatol.2015.1187","article-title":"Incidence Estimate of Nonmelanoma Skin Cancer (Keratinocyte carcinomas) in the US Population, 2012","volume":"151","author":"Rogers","year":"2015","journal-title":"JAMA Dermatol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1056\/NEJMra1708701","article-title":"Update on Keratinocyte Carcinomas","volume":"379","author":"Nehal","year":"2018","journal-title":"N. Engl. J. Med."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"6582","DOI":"10.1158\/1078-0432.CCR-14-1768","article-title":"Mutational Landscape of Aggressive Cutaneous Squamous Cell Carcinoma","volume":"20","author":"Pickering","year":"2014","journal-title":"Clin. Cancer Res."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1038\/s41419-021-04477-y","article-title":"Single-cell transcriptomic analysis reveals the critical molecular pattern of UV-induced cutaneous squamous cell carcinoma","volume":"13","author":"Yan","year":"2021","journal-title":"Cell Death Dis."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1038\/s41525-021-00226-4","article-title":"The landscape of driver mutations in cutaneous squamous cell carcinoma","volume":"6","author":"Chang","year":"2021","journal-title":"npj Genom. Med."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1016\/j.jaad.2020.04.088","article-title":"Validation of a 40-gene expression profile test to predict metastatic risk in localized high-risk cutaneous squamous cell carcinoma","volume":"84","author":"Wysong","year":"2021","journal-title":"J. Am. Acad. Dermatol."},{"key":"ref_9","first-page":"e46853","article-title":"40-Gene Expression Profile Representative of Metastatic Risk of Squamous Cell Carcinoma in a Mohs Surgical Patient","volume":"15","author":"Slater","year":"2023","journal-title":"Cureus"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1664","DOI":"10.1016\/j.jid.2020.12.024","article-title":"The Genomic Landscape of Actinic Keratosis","volume":"141","author":"Thomson","year":"2021","journal-title":"J. Investig. Dermatol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"416","DOI":"10.1186\/s12967-022-03622-1","article-title":"From Bowen disease to cutaneous squamous cell carcinoma: Eight markers were verified from transcriptomic and proteomic analyses","volume":"20","author":"Biao","year":"2022","journal-title":"J. Transl. Med."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1016\/j.pathol.2023.10.021","article-title":"Bowen disease is not synonymous with intraepidermal squamous cell carcinoma","volume":"56","author":"Wood","year":"2024","journal-title":"Pathology"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1111\/bjd.14852","article-title":"Current perspective on actinic keratosis: A review","volume":"177","author":"Siegel","year":"2017","journal-title":"Br. J. Dermatol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"S2","DOI":"10.1016\/j.jaad.2012.09.052","article-title":"Prevalence and awareness of actinic keratosis: Barriers and opportunities","volume":"68","author":"Rosen","year":"2013","journal-title":"J. Am. Acad. Dermatol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1111\/exd.14730","article-title":"Targeted deep sequencing reveals genomic alterations of actinic keratosis\/cutaneous squamous cell carcinoma in situ and cutaneous squamous cell carcinoma","volume":"32","author":"Kim","year":"2022","journal-title":"Exp. Dermatol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/j.jare.2022.11.008","article-title":"Extracellular matrix in skin diseases: The road to new therapies","volume":"51","author":"Malta","year":"2023","journal-title":"J. Adv. Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"R207","DOI":"10.1016\/j.cub.2017.02.006","article-title":"Basement membranes","volume":"27","author":"Jayadev","year":"2017","journal-title":"Curr. Biol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"892","DOI":"10.1038\/s41563-020-00894-0","article-title":"Basement membrane stiffness determines metastases formation","volume":"20","author":"Reuten","year":"2021","journal-title":"Nat. Mater."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"151220","DOI":"10.1016\/j.ejcb.2022.151220","article-title":"Multiple roles for basement membrane proteins in cancer progression and EMT","volume":"101","author":"Banerjee","year":"2022","journal-title":"Eur. J. Cell Biol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1146\/annurev-cellbio-111315-125227","article-title":"Cellular and Molecular Mechanisms of MT1-MMP-Dependent Cancer Cell Invasion","volume":"32","author":"Marchesin","year":"2016","journal-title":"Annu. Rev. Cell Dev. Biol."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Chen, K., Liu, S., Lu, C., and Gu, X. (2022). A prognostic and therapeutic hallmark developed by the integrated profile of basement membrane and immune infiltrative landscape in lung adenocarcinoma. Front. Immunol., 13.","DOI":"10.3389\/fimmu.2022.1058493"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Sun, W., Wang, J., Wang, Z., Xu, M., Lin, Q., Sun, P., and Yuan, Y. (2023). Combining WGCNA and machine learning to construct basement membrane-related gene index helps to predict the prognosis and tumor microenvironment of HCC patients and verifies the carcinogenesis of key gene CTSA. Front. Immunol., 14.","DOI":"10.3389\/fimmu.2023.1185916"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1645","DOI":"10.7150\/ijbs.81939","article-title":"Novel Implication of the Basement Membrane for Breast Cancer Outcome and Immune Infiltration","volume":"19","author":"Tian","year":"2023","journal-title":"Int. J. Biol. Sci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"eabn2265","DOI":"10.1126\/sciadv.abn2265","article-title":"A basement membrane discovery pipeline uncovers network complexity, regulators, and human disease associations","volume":"8","author":"Jayadev","year":"2022","journal-title":"Sci. Adv."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"592","DOI":"10.1038\/s41568-021-00376-8","article-title":"Decoding leader cells in collective cancer invasion","volume":"21","author":"Bocci","year":"2021","journal-title":"Nat. Rev. Cancer"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1392","DOI":"10.1038\/ncb1658","article-title":"Fibroblast-led collective invasion of carcinoma cells with differing roles for RhoGTPases in leading and following cells","volume":"9","author":"Gaggioli","year":"2007","journal-title":"Nat. Cell Biol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"eade2120","DOI":"10.1126\/sciadv.ade2120","article-title":"Fibroblasts generate topographical cues that steer cancer cell migration","volume":"9","author":"Baschieri","year":"2023","journal-title":"Sci. Adv."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"236","DOI":"10.4161\/cam.28733","article-title":"Assembly, heterogeneity, and breaching of the basement membranes","volume":"8","author":"Glentis","year":"2014","journal-title":"Cell Adhes. Migr."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1186\/s13062-024-00529-3","article-title":"Integrative genomics unveils basement membrane-related diagnostic markers and therapeutic targets in esophageal squamous cell carcinoma","volume":"19","author":"Zhang","year":"2024","journal-title":"Biol. Direct"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Bu, W., Cao, M., Wu, X., and Gao, Q. (2024). Prognosis prediction of head and neck squamous cell carcinoma through the basement membrane-related lncRNA risk model. Front. Mol. Biosci., 11.","DOI":"10.3389\/fmolb.2024.1421335"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"5120","DOI":"10.1038\/s41467-020-18794-x","article-title":"Concepts of extracellular matrix remodelling in tumour progression and metastasis","volume":"11","author":"Winkler","year":"2020","journal-title":"Nat. Commun."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1016\/j.devcel.2019.03.026","article-title":"The Extracellular Matrix Modulates the Metastatic Journey","volume":"49","author":"Kai","year":"2019","journal-title":"Dev. Cell"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"2456","DOI":"10.1083\/jcb.201903066","article-title":"Beyond proteases: Basement membrane mechanics and cancer invasion","volume":"218","author":"Chang","year":"2019","journal-title":"J. Cell Biol."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Bourgot, I., Primac, I., Louis, T., No\u00ebl, A., and Maquoi, E. (2020). Reciprocal Interplay Between Fibrillar Collagens and Collagen-Binding Integrins: Implications in Cancer Progression and Metastasis. Front. Oncol., 10.","DOI":"10.3389\/fonc.2020.01488"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1565","DOI":"10.1007\/s00438-022-01943-w","article-title":"FN1 encoding fibronectin as a pivotal signaling gene for therapeutic intervention against pancreatic cancer","volume":"297","author":"Ashok","year":"2022","journal-title":"Mol. Genet. Genom."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"14105","DOI":"10.1038\/ncomms14105","article-title":"Fibronectin-guided migration of carcinoma collectives","volume":"8","author":"Gopal","year":"2017","journal-title":"Nat. Commun."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"e3","DOI":"10.1017\/S1462399409001355","article-title":"Laminin-binding integrins and their tetraspanin partners as potential antimetastatic targets","volume":"12","author":"Stipp","year":"2010","journal-title":"Expert Rev. Mol. Med."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"2958","DOI":"10.1091\/mbc.e17-02-0126","article-title":"\u03b15\u03b21-integrinpromotes tension-dependent mammary epithelial cell invasion by engaging the fibronectin synergy site","volume":"28","author":"Miroshnikova","year":"2017","journal-title":"Mol. Biol. Cell"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"7431","DOI":"10.7150\/thno.76232","article-title":"Elevated ITGA5 facilitates hyperactivated mTORC1-mediated progression of laryngeal squamous cell carcinoma via upregulation of EFNB2","volume":"12","author":"Li","year":"2022","journal-title":"Theranostics"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1186\/s12964-023-01338-3","article-title":"Integrin\u03b2-1 in disorders and cancers: Molecular mechanisms and therapeutic targets","volume":"22","author":"Su","year":"2024","journal-title":"Cell Commun. Signal."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1523","DOI":"10.3892\/ijo.2013.2097","article-title":"Integrin alpha 6: A novel therapeutic target in esophageal squamous cell carcinoma","volume":"43","author":"Kwon","year":"2013","journal-title":"Int. J. Oncol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1007\/s10120-021-01239-9","article-title":"Integrin \u03b21 orchestrates the abnormal cell-matrix attachment and invasive behaviour of E-cadherin dysfunctional cells","volume":"25","author":"Figueiredo","year":"2021","journal-title":"Gastric Cancer"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1186\/s13045-018-0605-5","article-title":"The biology and role of CD44 in cancer progression: Therapeutic implications","volume":"11","author":"Chen","year":"2018","journal-title":"J. Hematol. Oncol."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Bourguignon, L.Y.W., and Bikle, D. (2015). Selective Hyaluronan\u00e2\u20ac\u201dCD44 Signaling Promotes miRNA-21 Expression and Interacts with Vitamin D Function during Cutaneous Squamous Cell Carcinomas Progression Following UV Irradiation. Front. Immunol., 6.","DOI":"10.3389\/fimmu.2015.00224"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1046\/j.1365-2133.2003.05028.x","article-title":"Hyaluronan, CD44 and versican in epidermal keratinocyte tumours","volume":"148","author":"Karvinen","year":"2003","journal-title":"Br. J. Dermatol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/bs.irn.2020.03.005","article-title":"Molecular determinants of the interaction between glioblastoma CD133+ cancer stem cells and the extracellular matrix","volume":"Volume 151","author":"Bryukhovetskiy","year":"2020","journal-title":"International Review of Neurobiology"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1056","DOI":"10.1111\/bjd.12816","article-title":"A truncating mutation in the laminin-332\u03b1 chain highlights the role of the LG45 proteolytic domain in regulating keratinocyte adhesion and migration","volume":"170","author":"Diociaiuti","year":"2014","journal-title":"Br. J. Dermatol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"e925735-1\u2013e925735-15","DOI":"10.12659\/MSM.925735","article-title":"Identification of Therapeutic and Prognostic Biomarkers of Lamin C (LAMC) Family Members in Head and Neck Squamous Cell Carcinoma","volume":"26","author":"Jiang","year":"2020","journal-title":"Med. Sci. Monit."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1186\/s13046-022-02516-w","article-title":"LAMC2 marks a tumor-initiating cell population with an aggressive signature in pancreatic cancer","volume":"41","author":"Cave","year":"2022","journal-title":"J. Exp. Clin. Cancer Res."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1016\/j.omtn.2019.08.005","article-title":"Long Non-coding RNA LINC00628 Interacts Epigenetically with the LAMA3 Promoter and Contributes to Lung Adenocarcinoma","volume":"18","author":"Xu","year":"2019","journal-title":"Mol. Ther. Nucleic Acids"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"eabe9716","DOI":"10.1126\/sciadv.abe9716","article-title":"Structural insights into integrin \u03b15\u03b21 opening by fibronectin ligand","volume":"7","author":"Schumacher","year":"2021","journal-title":"Sci. Adv."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"154179","DOI":"10.1016\/j.prp.2022.154179","article-title":"High FN1 expression correlates with gastric cancer progression","volume":"239","author":"Li","year":"2022","journal-title":"Pathol. Res. Pract."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"924","DOI":"10.1038\/s41467-017-00985-8","article-title":"Cancer-associated fibroblasts induce metalloprotease-independent cancer cell invasion of the basement membrane","volume":"8","author":"Glentis","year":"2017","journal-title":"Nat. Commun."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"100017","DOI":"10.1016\/j.xjidi.2021.100017","article-title":"Epidermal Integrin \u03b13\u03b21 Regulates Tumor-Derived Proteases BMP-1, Matrix Metalloprotease-9, and Matrix Metalloprotease-3","volume":"1","author":"Longmate","year":"2021","journal-title":"JID Innov."},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"van der Goot, F.G., Jiao, Y., Feng, X., Zhan, Y., Wang, R., Zheng, S., Liu, W., and Zeng, X. (2012). Matrix metalloproteinase-2 Promotes \u03b1v\u03b23 Integrin-Mediated Adhesion and Migration of Human Melanoma Cells by Cleaving Fibronectin. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0041591"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"34670","DOI":"10.18632\/oncotarget.16668","article-title":"Subcloning and characterization of highly metastatic cells derived from human esophageal squamous cell carcinoma KYSE150 cells by in vivo selection","volume":"8","author":"Okuda","year":"2017","journal-title":"Oncotarget"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1111\/jop.12025","article-title":"Epithelial and stromal syndecan-1 and -2 are distinctly expressed in oral- and cutaneous squamous cell carcinomas","volume":"42","author":"Omar","year":"2012","journal-title":"J. Oral Pathol. Med."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"e23354","DOI":"10.1096\/fj.202301287RR","article-title":"Single-cell sequencing of head and neck carcinoma: Transcriptional landscape and prognostic model based on malignant epithelial cell features","volume":"38","author":"Xiong","year":"2023","journal-title":"FASEB J."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"4388","DOI":"10.18632\/aging.202401","article-title":"Lumican inhibits immune escape and carcinogenic pathways in colorectal adenocarcinoma","volume":"13","author":"Zang","year":"2021","journal-title":"Aging"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"146461","DOI":"10.1016\/j.gene.2022.146461","article-title":"Expression and prognostic analysis of BGN in head and neck squamous cell carcinoma","volume":"827","author":"Zhao","year":"2022","journal-title":"Gene"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1111\/jop.13381","article-title":"Relationship of biglycan and decorin expression with clinicopathological features and prognosis in patients with oral squamous cell carcinoma","volume":"52","author":"Liu","year":"2022","journal-title":"J. Oral Pathol. Med."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1016\/S0901-5027(97)80869-7","article-title":"Down-regulated expression of CD44 variant 6 in oral squamous cell carcinomas and its relationship to regional lymph node metastasis","volume":"26","author":"Kunishi","year":"1999","journal-title":"Int. J. Oral Maxillofac. Surg."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1007\/s00795-018-0207-x","article-title":"Enhanced expression of nidogen 1 around the nest of basal cell carcinoma compared with that around squamous cell carcinoma","volume":"52","author":"Hirakawa","year":"2018","journal-title":"Med. Mol. Morphol."},{"key":"ref_64","first-page":"191","article-title":"Cutaneous squamous cell carcinoma: A comprehensive clinicopathologic classification. Part One","volume":"33","author":"Cassarino","year":"2007","journal-title":"J. Cutan. Pathol."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1097\/DAD.0b013e31827c7f31","article-title":"Lumican as a Novel Marker for Differential Diagnosis of Bowen Disease and Actinic Keratosis","volume":"35","author":"Takayama","year":"2013","journal-title":"Am. J. Dermatopathol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"i884","DOI":"10.1093\/bioinformatics\/bty560","article-title":"fastp: An ultra-fast all-in-one FASTQ preprocessor","volume":"34","author":"Chen","year":"2018","journal-title":"Bioinformatics"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"e47","DOI":"10.1093\/nar\/gkv007","article-title":"limma powers differential expression analyses for RNA-sequencing and microarray studies","volume":"43","author":"Ritchie","year":"2015","journal-title":"Nucleic Acids Res."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1038\/nprot.2008.211","article-title":"Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources","volume":"4","author":"Huang","year":"2008","journal-title":"Nat. Protoc."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1021\/acs.jproteome.8b00702","article-title":"Cytoscape StringApp: Network Analysis and Visualization of Proteomics Data","volume":"18","author":"Doncheva","year":"2018","journal-title":"J. Proteome Res."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1177\/1536867X1501500212","article-title":"A general-purpose nomogram generator for predictive logistic regression models","volume":"15","author":"Zlotnik","year":"2015","journal-title":"Stata J."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/3\/1394\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T13:06:02Z","timestamp":1769778362000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/3\/1394"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,30]]},"references-count":70,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2026,2]]}},"alternative-id":["ijms27031394"],"URL":"https:\/\/doi.org\/10.3390\/ijms27031394","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,30]]}}}