{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T17:03:56Z","timestamp":1769965436538,"version":"3.49.0"},"reference-count":79,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T00:00:00Z","timestamp":1769731200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Science Centre, Poland","award":["UMO-2019\/35\/B\/NZ9\/01567"],"award-info":[{"award-number":["UMO-2019\/35\/B\/NZ9\/01567"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The growing interest in sustainable and structurally diverse sorbent materials has intensified the search for effective biosorbents that can complement or replace conventional adsorbents. This work presents the potential use of Chlorella vulgaris dried biomass and its modifications (ultrasound-treated, lipid-extracted, and combined forms) for diclofenac (DCF) sorption from aqueous solutions. It was demonstrated that sorption efficiency significantly depends on the solution\u2019s pH. Lowering the pH from 6 to 2 increases the sorption from 5% to 68%, while 99% desorption occurred at pH 9. The adsorption isotherms for intact biomass and after lipid extraction (CV-E2) are best described by the Langmuir and Freundlich models; for ultrasonically treated biomass (CV-E1) by the Temkin model; and for ultrasound-assisted solvent extraction (CV-E3) by the Dubinin\u2013Radushkevich model. These findings demonstrate that cellular lipids and particle characteristics critically govern sorption mechanisms, highlighting dried Chlorella biomass as a structurally and chemically tunable biosorbent. Importantly, the key sorption experiments were performed under strongly acidic conditions (pH 2), which differ from typical wastewater or surface water matrices. Therefore, the presented results should be regarded as a proof of concept illustrating the mechanistic potential of dried Chlorella biomass as a tunable sorptive material, with prospective relevance for separation science and laboratory-scale analytical applications rather than direct environmental remediation.<\/jats:p>","DOI":"10.3390\/ijms27031413","type":"journal-article","created":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T17:38:34Z","timestamp":1769794714000},"page":"1413","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Investigations of Diclofenac Sorption on Intact and Modified Chlorella vulgaris Biomass with pH-Switchable Desorption"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2125-3608","authenticated-orcid":false,"given":"Ivan","family":"Liakh","sequence":"first","affiliation":[{"name":"Department of Toxicology, Faculty of Pharmacy, Medical University of Gda\u0144sk, Al. Gen. Hallera 107, 80-416 Gdansk, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6325-6906","authenticated-orcid":false,"given":"Adrian","family":"Szewczyk","sequence":"additional","affiliation":[{"name":"Department of Physical Chemistry, Faculty of Pharmacy, Medical University of Gda\u0144sk, Al. Gen. Hallera 107, 80-416 Gdansk, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6099-1024","authenticated-orcid":false,"given":"Magdalena","family":"Prokopowicz","sequence":"additional","affiliation":[{"name":"Department of Physical Chemistry, Faculty of Pharmacy, Medical University of Gda\u0144sk, Al. Gen. Hallera 107, 80-416 Gdansk, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9806-8844","authenticated-orcid":false,"given":"Magdalena","family":"Narajczyk","sequence":"additional","affiliation":[{"name":"Bioimaging Laboratory, Faculty of Biology, University of Gda\u0144sk, Ul. Wita Stwosza 59, 80-308 Gdansk, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4766-2434","authenticated-orcid":false,"given":"Anna","family":"Aksmann","sequence":"additional","affiliation":[{"name":"Department of Plant Experimental Biology and Biotechnology, Faculty of Biology, University of Gda\u0144sk, Ul. Wita Stwosza 59, 80-308 Gdansk, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8705-1438","authenticated-orcid":false,"given":"Darya","family":"Harshkova","sequence":"additional","affiliation":[{"name":"Department of Plant Experimental Biology and Biotechnology, Faculty of Biology, University of Gda\u0144sk, Ul. Wita Stwosza 59, 80-308 Gdansk, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4245-9197","authenticated-orcid":false,"given":"Bartosz","family":"Wielgomas","sequence":"additional","affiliation":[{"name":"Department of Toxicology, Faculty of Pharmacy, Medical University of Gda\u0144sk, Al. Gen. Hallera 107, 80-416 Gdansk, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,1,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1016\/j.aquatox.2005.09.009","article-title":"Ecotoxicology of Human Pharmaceuticals","volume":"76","author":"Fent","year":"2006","journal-title":"Aquat. Toxicol."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Hejna, M., Kapu\u015bci\u0144ska, D., and Aksmann, A. (2022). Pharmaceuticals in the Aquatic Environment: A Review on Eco-Toxicology and the Remediation Potential of Algae. Int. J. Environ. Res. Public Health, 19.","DOI":"10.3390\/ijerph19137717"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/j.envint.2016.09.014","article-title":"Diclofenac and Its Transformation Products: Environmental Occurrence and Toxicity\u2014A Review","volume":"96","author":"Lonappan","year":"2016","journal-title":"Environ. Int."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Hern\u00e1ndez-Zamora, M., Cruz-Castillo, L.M., Mart\u00ednez-Jer\u00f3nimo, L., and Mart\u00ednez-Jer\u00f3nimo, F. (2025). Diclofenac Produces Diverse Toxic Effects on Aquatic Organisms of Different Trophic Levels, Including Microalgae, Cladocerans, and Fish. Water, 17.","DOI":"10.3390\/w17101489"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1465","DOI":"10.1016\/j.jece.2017.02.018","article-title":"Removal of Sodium Diclofenac from Aqueous Solution by Adsorbents Derived from Cocoa Pod Husks","volume":"5","author":"Murniati","year":"2017","journal-title":"J. Environ. Chem. Eng."},{"key":"ref_6","first-page":"32","article-title":"Adsorption of Diclofenac from Aqueous Solution Using Cyclamen persicum Tubers Based Activated Carbon (CTAC)","volume":"20","author":"Jodeh","year":"2016","journal-title":"J. Assoc. Arab Univ. Basic Appl. Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"10380","DOI":"10.1016\/j.ijhydene.2016.01.096","article-title":"Adsorption of Diclofenac from Aqueous Solution Using Activated Carbon Prepared from Olive Stones","volume":"41","author":"Larous","year":"2016","journal-title":"Int. J. Hydrogen Energy"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"7318","DOI":"10.1007\/s11356-019-06844-x","article-title":"Modified Biochar from Moringa Seed Powder for the Removal of Diclofenac from Aqueous Solution","volume":"27","author":"Bagheri","year":"2020","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"74521","DOI":"10.1007\/s11356-023-27767-8","article-title":"Algal Sorbents and Prospects for Their Application in the Sustainable Recovery of Rare Earth Elements from E-Waste","volume":"30","author":"Pinto","year":"2023","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1080\/26388081.2022.2158132","article-title":"Live Algal Sorbents for the Removal of Potentially Toxic Elements: A Review","volume":"4","author":"Read","year":"2023","journal-title":"Appl. Phycol."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Touliabah, H.E.-S., El-Sheekh, M.M., Ismail, M.M., and El-Kassas, H. (2022). A Review of Microalgae- and Cyanobacteria-Based Biodegradation of Organic Pollutants. Molecules, 27.","DOI":"10.3390\/molecules27031141"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1002\/jctb.7517","article-title":"Chemical Activation of Microcystis aeruginosa Biomass: A Promising Approach for Enhanced Diclofenac Sorption and Water Treatment","volume":"99","author":"Momin","year":"2024","journal-title":"J. Chem. Technol. Biotechnol."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Santos, C.E., de Coimbra, R.N., Bermejo, S.P., P\u2019erez, A.I.G., and Cabero, M.O. (2017). Comparative Assessment of Pharmaceutical Removal from Wastewater by the Microalgae Chlorella sorokiniana, Chlorella vulgaris and Scenedesmus obliquus. Biological Wastewater Treatment and Resource Recovery, InTech.","DOI":"10.5772\/66772"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1080\/07388550500248571","article-title":"Use of Algae for Removing Heavy Metal Ions from Wastewater: Progress and Prospects","volume":"25","author":"Mehta","year":"2005","journal-title":"Crit. Rev. Biotechnol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1007\/s11274-019-2648-3","article-title":"Algae as a Green Technology for Heavy Metals Removal from Various Wastewater","volume":"35","author":"Salama","year":"2019","journal-title":"World J. Microbiol. Biotechnol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.biortech.2014.01.068","article-title":"A Comprehensive Review on Biosorption of Heavy Metals by Algal Biomass: Materials, Performances, Chemistry, and Modeling Simulation Tools","volume":"160","author":"He","year":"2014","journal-title":"Bioresour. Technol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1007\/s00284-007-9070-z","article-title":"Bioaccumulation of Heavy Metals by Green Algae","volume":"56","author":"Doshi","year":"2008","journal-title":"Curr. Microbiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1111\/j.1574-6968.1999.tb13386.x","article-title":"Biodegradation of Aromatic Compounds by Microalgae","volume":"170","author":"Semple","year":"1999","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"106594","DOI":"10.1016\/j.envint.2021.106594","article-title":"Microalgae-Based Technology for Antibiotics Removal: From Mechanisms to Application of Innovational Hybrid Systems","volume":"155","author":"Xiong","year":"2021","journal-title":"Environ. Int."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"124041","DOI":"10.1016\/j.jhazmat.2020.124041","article-title":"Removal of Pharmaceutical and Personal Care Products (PPCPs) from Wastewater Using Microalgae: A Review","volume":"403","author":"Hena","year":"2021","journal-title":"J. Hazard. Mater."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Bano, F., Malik, A., and Ahammad, S.Z. (2021). Removal of Estradiol, Diclofenac, and Triclosan by Naturally Occurring Microalgal Consortium Obtained from Wastewater. Sustainability, 13.","DOI":"10.3390\/su13147690"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"69242","DOI":"10.1007\/s11356-021-15505-x","article-title":"Biosorption of Ketoprofen and Diclofenac by Living Cells of the Green Microalgae Chlorella sp","volume":"28","author":"Hifney","year":"2021","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1519","DOI":"10.32604\/phyton.2021.015087","article-title":"Photodegradation and Removal of Diclofenac by the Green Alga Nannochloropsis oculata","volume":"90","year":"2021","journal-title":"Phyton"},{"key":"ref_24","first-page":"210","article-title":"Diclofenac Removal by the Microalgae Species Chlorella vulgaris, Nannochloropsis oculata, Scenedesmus acutus, and Scenedesmus obliquus","volume":"12","year":"2022","journal-title":"3 Biotech"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1416","DOI":"10.1002\/jctb.6911","article-title":"Cell Growth and Removal Capacity of Ibuprofen and Diclofenac by Parachlorella kessleri at Bench Scale","volume":"97","year":"2022","journal-title":"J. Chem. Technol. Biotechnol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"109769","DOI":"10.1016\/j.ecoenv.2019.109769","article-title":"Biodegradation of Diclofenac by Two Green Microalgae: Picocystis sp. and Graesiella sp","volume":"186","author":"Cimetiere","year":"2019","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1357","DOI":"10.4014\/jmb.2209.09012","article-title":"Biotechnological Approaches for Biomass and Lipid Production Using Microalgae Chlorella and Its Future Perspectives","volume":"32","author":"Je","year":"2022","journal-title":"J. Microbiol. Biotechnol."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Bito, T., Okumura, E., Fujishima, M., and Watanabe, F. (2020). Potential of Chlorella as a Dietary Supplement to Promote Human Health. Nutrients, 12.","DOI":"10.3390\/nu12092524"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Aci\u00e9n, F.G., Fern\u00e1ndez, J.M., and Molina-Grima, E. (2014). Economics of Microalgae Biomass Production. Biofuels from Algae, Elsevier.","DOI":"10.1016\/B978-0-444-59558-4.00014-0"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Ashour, M., Mansour, A.T., Alkhamis, Y.A., and Elshobary, M. (2024). Usage of Chlorella and Diverse Microalgae for CO2 Capture\u2014Towards a Bioenergy Revolution. Front. Bioeng. Biotechnol., 12.","DOI":"10.3389\/fbioe.2024.1387519"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"133057","DOI":"10.1016\/j.chemosphere.2021.133057","article-title":"Integrating Anaerobic Digestion and Microalgae Cultivation for Dairy Wastewater Treatment and Potential Biochemicals Production from the Harvested Microalgal Biomass","volume":"291","author":"Kusmayadi","year":"2022","journal-title":"Chemosphere"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Zakaria, S.M., Kamal, S.M.M., Harun, M.R., Omar, R., and Siajam, S.I. (2017). Subcritical Water Technology for Extraction of Phenolic Compounds from Chlorella sp. Microalgae and Assessment on Its Antioxidant Activity. Molecules, 22.","DOI":"10.3390\/molecules22071105"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1227","DOI":"10.1002\/ceat.202100008","article-title":"Extraction of Chlorophylls and Carotenoids from Microalgae: COSMO-SAC-Assisted Solvent Screening","volume":"44","author":"Frankenberg","year":"2021","journal-title":"Chem. Eng. Technol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"134615","DOI":"10.1016\/j.foodchem.2022.134615","article-title":"Pulsed Electric Field (PEF) Recovery of Biomolecules from Chlorella: Extract Efficiency, Nutrient Relative Value, and Algae Morphology Analysis","volume":"404","author":"Wang","year":"2023","journal-title":"Food Chem."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"864","DOI":"10.1016\/j.ultsonch.2010.11.016","article-title":"The Characterization of Acoustic Cavitation Bubbles\u2014An Overview","volume":"18","author":"Ashokkumar","year":"2011","journal-title":"Ultrason. Sonochem."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1439","DOI":"10.1126\/science.247.4949.1439","article-title":"Sonochemistry","volume":"247","author":"Suslick","year":"1990","journal-title":"Science"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"102517","DOI":"10.1016\/j.jwpe.2021.102517","article-title":"Fluorescence Descriptors for Algal Organic Matter and Microalgae Disintegration during Ultrasonication","volume":"45","author":"Khan","year":"2022","journal-title":"J. Water Process Eng."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1007\/s00128-018-2348-y","article-title":"Effects of Low-Frequency Ultrasound on Microcystis aeruginosa from Cell Inactivation to Disruption","volume":"101","author":"Tan","year":"2018","journal-title":"Bull. Environ. Contam. Toxicol."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"An, J., Miao, X., Wang, L., Li, X., Liu, X., and Gao, H. (2021). Visualizing the Integrity of Chloroplast Envelope by Rhodamine and Nile Red Staining. Front. Plant Sci., 12.","DOI":"10.3389\/fpls.2021.668414"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"e462","DOI":"10.1002\/pld3.462","article-title":"Live-cell Imaging of the Chloroplast Outer Envelope Membrane Using Fluorescent Dyes","volume":"6","author":"Ichikawa","year":"2022","journal-title":"Plant Direct."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/S1387-1811(02)00545-0","article-title":"Nomenclature of Structural and Compositional Characteristics of Ordered Microporous and Mesoporous Materials with Inorganic Hosts","volume":"58","author":"McCusker","year":"2003","journal-title":"Microporous Mesoporous Mater."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"4499","DOI":"10.1016\/j.biortech.2010.01.065","article-title":"Using FTIR Spectroscopy for Rapid Determination of Lipid Accumulation in Response to Nitrogen Limitation in Freshwater Microalgae","volume":"101","author":"Dean","year":"2010","journal-title":"Bioresour. Technol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1007\/s12011-020-02055-5","article-title":"Green Synthesis of CuFe2O4@Ag Nanocomposite Using the Chlorella vulgaris and Evaluation of Its Effect on the Expression of NorA Efflux Pump Gene Among Staphylococcus aureus Strains","volume":"198","author":"Kahzad","year":"2020","journal-title":"Biol. Trace Elem. Res."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1807","DOI":"10.1002\/jctb.4027","article-title":"Optimization of Lipid Production for Algal Biodiesel in Nitrogen Stressed Cells of Dunaliella salina Using FTIR Analysis","volume":"88","author":"Liu","year":"2013","journal-title":"J. Chem. Technol. Biotechnol."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Forfang, K., Zimmermann, B., Kosa, G., Kohler, A., and Shapaval, V. (2017). FTIR Spectroscopy for Evaluation and Monitoring of Lipid Extraction Efficiency for Oleaginous Fungi. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0170611"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1016\/j.ecoenv.2018.01.012","article-title":"Removal of Pharmaceutical Pollutants from Synthetic Wastewater Using Chemically Modified Biomass of Green Alga Scenedesmus obliquus","volume":"151","author":"Ali","year":"2018","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Silva, A., Coimbra, R.N., Escapa, C., Figueiredo, S.A., Freitas, O.M., and Otero, M. (2020). Green Microalgae Scenedesmus obliquus Utilization for the Adsorptive Removal of Nonsteroidal Anti-Inflammatory Drugs (NSAIDs) from Water Samples. Int. J. Environ. Res. Public Health, 17.","DOI":"10.3390\/ijerph17103707"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Coimbra, R.N., Escapa, C., V\u00e1zquez, N.C., Noriega-Hevia, G., and Otero, M. (2018). Utilization of Non-Living Microalgae Biomass from Two Different Strains for the Adsorptive Removal of Diclofenac from Water. Water, 10.","DOI":"10.3390\/w10101401"},{"key":"ref_49","first-page":"1","article-title":"Chlorella vulgaris Biomass Enriched by Biosorption of Polyphenols","volume":"10","author":"Quintelas","year":"2015","journal-title":"Algal Res."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"211","DOI":"10.17512\/ios.2017.2.6","article-title":"The Effect of Biomass (Chlorella vulgaris, Scenedesmus armatus) Concentrations on Zn 2+, Pb 2+ and Cd 2+ Biosorption from Zinc Smelting Wastewater","volume":"20","year":"2017","journal-title":"Eng. Prot. Environ."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"899","DOI":"10.1007\/s10098-018-1497-3","article-title":"Algae-Based Sorbents for Removal of Gallium from Semiconductor Manufacturing Wastewater","volume":"20","author":"Li","year":"2018","journal-title":"Clean Technol. Environ. Policy"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/j.algal.2016.06.008","article-title":"Comparative Assessment of Diclofenac Removal from Water by Different Microalgae Strains","volume":"18","author":"Escapa","year":"2016","journal-title":"Algal Res."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1016\/j.jhazmat.2015.10.033","article-title":"Micropollutant Removal in an Algal Treatment System Fed with Source Separated Wastewater Streams","volume":"304","author":"Butkovskyi","year":"2016","journal-title":"J. Hazard. Mater."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"2018","DOI":"10.1039\/C4EM00094C","article-title":"Simultaneous Removal of Inorganic and Organic Compounds in Wastewater by Freshwater Green Microalgae","volume":"16","author":"Zhou","year":"2014","journal-title":"Environ. Sci. Process Impacts"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.jenvman.2012.06.042","article-title":"Bioaccumulation and Biosorption of Copper and Lead by a Unicellular Algae Chlamydomonas reinhardtii in Single and Binary Metal Systems: A Comparative Study","volume":"111","author":"Flouty","year":"2012","journal-title":"J. Environ. Manag."},{"key":"ref_56","first-page":"1","article-title":"Utilization of Chlorella vulgaris after the Extraction Process in Wastewater Treatment as a Biosorption Material for Ciprofloxacin Removal","volume":"24","year":"2023","journal-title":"J. Ecol. Eng."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"14128","DOI":"10.1021\/acsomega.2c00696","article-title":"Enhanced Tetracycline Removal from Highly Concentrated Aqueous Media by Lipid-Free Chlorella sp. Biomass","volume":"7","year":"2022","journal-title":"ACS Omega"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.biortech.2018.02.079","article-title":"Bioremediation of Cephalexin with Non-Living Chlorella sp., Biomass after Lipid Extraction","volume":"257","author":"Angulo","year":"2018","journal-title":"Bioresour. Technol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/j.carbon.2003.09.022","article-title":"Adsorption of Organic Molecules from Aqueous Solutions on Carbon Materials","volume":"42","year":"2004","journal-title":"Carbon"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1002\/etc.3339","article-title":"Pharmaceuticals in the Environment\u2014Global Occurrences and Perspectives","volume":"35","author":"Weber","year":"2015","journal-title":"Environ. Toxicol. Chem."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"6086","DOI":"10.1016\/j.watres.2013.07.027","article-title":"PH Dynamics in Sewers and Its Modeling","volume":"47","author":"Sharma","year":"2013","journal-title":"Water Res."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/j.micromeso.2018.07.046","article-title":"Aminopropyl-Functionalized Mesoporous Silica SBA-15 as Drug Carrier for Cefazolin: Adsorption Profiles, Release Studies, and Mineralization Potential","volume":"274","author":"Szewczyk","year":"2019","journal-title":"Microporous Mesoporous Mater."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"646","DOI":"10.1016\/j.molliq.2019.01.005","article-title":"Application of Dubinin\u2013Radushkevich Isotherm Model at the Solid\/Solution Interface: A Theoretical Analysis","volume":"277","author":"Hu","year":"2019","journal-title":"J. Mol. Liq."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"2154","DOI":"10.2134\/jeq2006.0013","article-title":"The Role of Lipids on Sorption Characteristics of Freshwater\u2014And Wastewater-Irrigated Soils","volume":"35","author":"Drori","year":"2006","journal-title":"J. Environ. Qual."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"929","DOI":"10.1016\/S0146-6380(99)00076-5","article-title":"Contribution of Lipids to the Nonlinear Sorption of Polycyclic Aromatic Hydrocarbons to Soil Organic Matter","volume":"30","author":"Kohl","year":"1999","journal-title":"Org. Geochem."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"1609","DOI":"10.1016\/j.chemosphere.2004.11.073","article-title":"Effects of Lipids on the Sorption of Hydrophobic Organic Compounds on Geosorbents: A Case Study Using Phenanthrene","volume":"58","author":"Tremblay","year":"2005","journal-title":"Chemosphere"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"20051","DOI":"10.1039\/C5RA27264E","article-title":"Biosorption of Cadmium by a Lipid Extraction Residue of Lipid-Rich Microalgae","volume":"6","author":"Zheng","year":"2016","journal-title":"RSC Adv."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"310","DOI":"10.1016\/j.cej.2012.09.099","article-title":"Removal of Pharmaceutical Compounds by Activated Carbon Prepared from Agricultural By-Product","volume":"211\u2013212","author":"Baccar","year":"2012","journal-title":"Chem. Eng. J."},{"key":"ref_69","doi-asserted-by":"crossref","unstructured":"\u015acigalski, P., and Kosobucki, P. (2024). Dendrimer Coated Silica as a Sorbent for Dispersive Solid-Phase Extraction of Select Non-Steroidal Anti-Inflammatory Drugs from Water. Molecules, 29.","DOI":"10.3390\/molecules29020380"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"115494","DOI":"10.1016\/j.envpol.2020.115494","article-title":"Use of Nanocellulose Extracted from Grass for Adsorption Abatement of Ciprofloxacin and Diclofenac Removal with Phyto, and Fish Toxicity Studies","volume":"268","author":"Shahnaz","year":"2021","journal-title":"Environ. Pollut."},{"key":"ref_71","first-page":"78","article-title":"Optimal Removal of Diclofenac and Amoxicillin by Activated Carbon Prepared from Coconut Shell through Response Surface Methodology","volume":"38","author":"Daouda","year":"2021","journal-title":"S. Afr. J. Chem. Eng."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"113200","DOI":"10.1016\/j.indcrop.2020.113200","article-title":"Adsorption of Non-Steroidal Anti-Inflammatory Drug (NSAID) by Agro-Industrial by-Product with Chemical and Thermal Modification: Adsorption Studies and Mechanism","volume":"161","author":"Quesada","year":"2021","journal-title":"Ind. Crops Prod."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"121284","DOI":"10.1016\/j.jhazmat.2019.121284","article-title":"Effective Adsorption of Diclofenac Sodium from Neutral Aqueous Solution by Low-Cost Lignite Activated Cokes","volume":"384","author":"Wu","year":"2020","journal-title":"J. Hazard. Mater."},{"key":"ref_74","doi-asserted-by":"crossref","unstructured":"Fischer, K., Sydow, S., Griebel, J., Naumov, S., Elsner, C., Thomas, I., Abdul Latif, A., and Schulze, A. (2020). Enhanced Removal and Toxicity Decline of Diclofenac by Combining UVA Treatment and Adsorption of Photoproducts to Polyvinylidene Difluoride. Polymers, 12.","DOI":"10.3390\/polym12102340"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"5553212","DOI":"10.1155\/2022\/5553212","article-title":"Improper Estimation of Thermodynamic Parameters in Adsorption Studies with Distribution Coefficient KD (qe\/Ce) or Freundlich Constant (KF): Considerations from the Derivation of Dimensionless Thermodynamic Equilibrium Constant and Suggestions","volume":"2022","author":"Tran","year":"2022","journal-title":"Adsorpt. Sci. Technol."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"1981","DOI":"10.1021\/je800661q","article-title":"Is the Free Energy Change of Adsorption Correctly Calculated?","volume":"54","author":"Liu","year":"2009","journal-title":"J. Chem. Eng. Data"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"1275","DOI":"10.1080\/01496395.2023.2189050","article-title":"Misinterpretation of Dubinin\u2013Radushkevich Isotherm and Its Implications on Adsorption Parameter Estimates","volume":"58","author":"Mahanty","year":"2023","journal-title":"Sep. Sci. Technol."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"100376","DOI":"10.1016\/j.colcom.2021.100376","article-title":"On the Use of the Dubinin-Radushkevich Equation to Distinguish between Physical and Chemical Adsorption at the Solid-Water Interface","volume":"41","author":"Puccia","year":"2021","journal-title":"Colloid Interface Sci. Commun."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"104267","DOI":"10.1016\/j.arabjc.2022.104267","article-title":"Some Consideration Triggered by Misquotation of Temkin Model and the Derivation of Its Correct Form","volume":"15","author":"Zhou","year":"2022","journal-title":"Arab. J. Chem."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/3\/1413\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T05:12:32Z","timestamp":1769922752000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/3\/1413"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,30]]},"references-count":79,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2026,2]]}},"alternative-id":["ijms27031413"],"URL":"https:\/\/doi.org\/10.3390\/ijms27031413","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,30]]}}}