{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T21:37:46Z","timestamp":1791581866044,"version":"4.3.4"},"reference-count":81,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de Nivel Superior","doi-asserted-by":"crossref","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Brazilian National Council of Scientific and Technological Development","award":["09890\/2022-5"],"award-info":[{"award-number":["09890\/2022-5"]}]},{"name":"Brazilian National Council of Scientific and Technological Development","award":["408598\/2023-9"],"award-info":[{"award-number":["408598\/2023-9"]}]},{"name":"Brazilian National Council of Scientific and Technological Development","award":["310532\/2022-1"],"award-info":[{"award-number":["310532\/2022-1"]}]},{"name":"Brazilian National Council of Scientific and Technological Development","award":["403665\/2020-5"],"award-info":[{"award-number":["403665\/2020-5"]}]},{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","award":["APQ-05316-23"],"award-info":[{"award-number":["APQ-05316-23"]}],"id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Tocantins State Foundation for Research Aid"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Brown spot, caused by the seedborne fungus Bipolaris oryzae, remains a major constraint in rice production. Here, we used in vitro and in vivo assays to evaluate the biocontrol potential of three Bacillus strains (Ba. cereus OQ725688.1, Ba. velezensis OP938696.1, and Ba. subtilis OP937353.1) against Bi. oryzae in two rice cultivars (\u201cRubelita\u201d and \u201cPredileta\u201d). Ba. cereus showed the highest in vitro mycelial inhibition (\u224895%), whereas Ba. velezensis was the most effective under greenhouse conditions, reducing disease severity by up to 60% and increasing seedling vigor by 51% compared with infected controls. \u201cPredileta\u201d showed the strongest response to bacterial treatment, maintaining severity scores below 2 even under high inoculum pressure. Functional assays confirmed that all strains displayed amylolytic, catalase, and phosphate-solubilizing activities, with Ba. velezensis uniquely expressing strong cellulase and protease activities. Genome analysis of Ba. velezensis OP938696.1 revealed multiple biosynthetic gene clusters for antifungal polyketides and lipopeptides. These integrated biochemical and genomic traits demonstrate the novelty and potential of this Neotropical strain as a multifunctional agent capable of suppressing Bi. oryzae while enhancing rice seedling performance. Incorporating such a native strain into seed and soil management offers a sustainable strategy for rice protection in Neotropical systems.<\/jats:p>","DOI":"10.3390\/ijms27031455","type":"journal-article","created":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T09:00:33Z","timestamp":1770022833000},"page":"1455","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Bacillus velezensis Enhances Rice Resistance to Brown Spot by Integrating Antifungal and Growth Promotion Functions"],"prefix":"10.3390","volume":"27","author":[{"given":"Elizabeth B. E.","family":"Pires","sequence":"first","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Biotecnologia, Universidade Federal do Tocantins (UFT), Gurupi 77402-970, TO, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5396-7053","authenticated-orcid":false,"given":"Maira S.","family":"Tique Obando","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Biotecnologia, Universidade Federal do Tocantins (UFT), Gurupi 77402-970, TO, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5772-0477","authenticated-orcid":false,"given":"Luis","family":"Janssen","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Biotecnologia, Universidade Federal do Tocantins (UFT), Gurupi 77402-970, TO, Brazil"},{"name":"Departamento de Biologia Celular, Universitdade de Bras\u00edlia, Bras\u00edlia 70297-400, DF, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bergmann M.","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"Departamento de Biologia Celular, Universitdade de Bras\u00edlia, Bras\u00edlia 70297-400, DF, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8333-7984","authenticated-orcid":false,"given":"Odaiza F.","family":"Souza","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Biotecnologia, Universidade Federal do Tocantins (UFT), Gurupi 77402-970, TO, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcelo L.","family":"Dias","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-gradua\u00e7\u00e3o em Biodiversidade e Biotecnologia\u2014Rede Bionorte, Universidade Federal do Tocantins (UFT), Gurupi 77402-970, TO, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0164-505X","authenticated-orcid":false,"given":"Lu\u00eds O.","family":"Viteri Jumbo","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Biotecnologia, Universidade Federal do Tocantins (UFT), Gurupi 77402-970, TO, Brazil"},{"name":"Programa de P\u00f3s-gradua\u00e7\u00e3o em Produ\u00e7\u00e3o Vegetal, Universidade Federal do Tocantins, Gurupi 77410-530, TO, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodrigo R.","family":"Fidelis","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-gradua\u00e7\u00e3o em Produ\u00e7\u00e3o Vegetal, Universidade Federal do Tocantins, Gurupi 77410-530, TO, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3830-9463","authenticated-orcid":false,"given":"Gil R.","family":"Santos","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Biotecnologia, Universidade Federal do Tocantins (UFT), Gurupi 77402-970, TO, Brazil"},{"name":"Programa de P\u00f3s-gradua\u00e7\u00e3o em Produ\u00e7\u00e3o Vegetal, Universidade Federal do Tocantins, Gurupi 77410-530, TO, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raimundo N. C.","family":"Rocha","sequence":"additional","affiliation":[{"name":"Embrapa Arroz e Feij\u00e3o, Santo Ant\u00f4nio de Goi\u00e1s 75375-000, GO, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8334-3313","authenticated-orcid":false,"given":"Guy","family":"Smagghe","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Biotecnologia, Universidade Federal do Tocantins (UFT), Gurupi 77402-970, TO, Brazil"},{"name":"Institute of Entomology, Guizhou University, Guiyang 550025, China"},{"name":"Department of Biology, Vrije Universiteit Brussel (VUB), 1050 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tito","family":"Bacca","sequence":"additional","affiliation":[{"name":"Facultad de Ingenier\u00eda Agron\u00f3mica, Universidad del Tolima, Ibagu\u00e9 730001, Tolima, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1174-6564","authenticated-orcid":false,"given":"Eugenio E.","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Biotecnologia, Universidade Federal do Tocantins (UFT), Gurupi 77402-970, TO, Brazil"},{"name":"Departamento de Entomologia, Universidade Federal de Vi\u00e7osa, Vi\u00e7osa 36570-900, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2327-7120","authenticated-orcid":false,"given":"Rudolf","family":"Haumann","sequence":"additional","affiliation":[{"name":"Department of Bioprocess Engineering, Institute of Food Science and Biotechnology, University of Hohenheim, 70599 Stuttgart, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raimundo W. S.","family":"Aguiar","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Biotecnologia, Universidade Federal do Tocantins (UFT), Gurupi 77402-970, TO, Brazil"},{"name":"Programa de P\u00f3s-gradua\u00e7\u00e3o em Biodiversidade e Biotecnologia\u2014Rede Bionorte, Universidade Federal do Tocantins (UFT), Gurupi 77402-970, TO, Brazil"},{"name":"Programa de P\u00f3s-gradua\u00e7\u00e3o em Produ\u00e7\u00e3o Vegetal, Universidade Federal do Tocantins, Gurupi 77410-530, TO, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,2,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Abeysekara, I., and Rathnayake, I. (2025, November 13). Global Trends in Rice Production, Consumption and Trade. 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