{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:49:38Z","timestamp":1787028578940,"version":"build-2736575974"},"reference-count":65,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T00:00:00Z","timestamp":1770076800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["24-25-00481"],"award-info":[{"award-number":["24-25-00481"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Obesity is a major risk factor for heart failure and atrial fibrillation. This study investigated the effects of diet-induced obesity on the molecular and cellular mechanisms of cardiomyocyte contractility in the left and right atria (LA and RA). Female Wistar rats were fed a Western diet (WD) for 18 weeks. Sarcomere dynamics and calcium transients were measured in unloaded cardiomyocytes. Actin\u2013myosin interactions and contractile protein phosphorylation were assessed via an in vitro motility assay and phosphoprotein-specific gel electrophoresis. WD-fed rats developed obesity, hypertension, and metabolic alterations in the absence of echocardiographic or histological evidence of cardiac remodeling or systolic dysfunction. In LA cardiomyocytes, contractile dysfunction was indicated by increased calcium transient amplitude coupled with reduced shortening amplitude and relengthening velocity. This functional impairment correlated with a slowed myosin cross-bridge cycle and dephosphorylation of cMyBP-C. In contrast, RA cardiomyocytes displayed only molecular changes in response to obesity, including altered phosphorylation of most sarcomeric proteins and a decelerated cross-bridge cycle, but showed no evident contractile dysfunction. Thus, an 18-week WD reflects the early stages of contractile impairment, where functional deficits are specific to the LA, while RA alterations are confined to the molecular level.<\/jats:p>","DOI":"10.3390\/ijms27031508","type":"journal-article","created":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T16:46:28Z","timestamp":1770137188000},"page":"1508","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Western Diet-Induced Impairment of Left Atrium Cardiomyocyte Contractility in Female Wistar Rats Is Associated with Slowdown in the Cross-Bridge Cycle and Dephosphorylation of cMyBP-C"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5159-4465","authenticated-orcid":false,"given":"Elena","family":"Mukhlynina","sequence":"first","affiliation":[{"name":"Institute of Immunology and Physiology, Russian Academy of Sciences, Pervomayskaya Str. 106, 620078 Yekaterinburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4432-8863","authenticated-orcid":false,"given":"Xenia","family":"Butova","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Problems RAS, Khoroshevskoye sh., 76A, 123007 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3188-9992","authenticated-orcid":false,"given":"Tatiana","family":"Myachina","sequence":"additional","affiliation":[{"name":"Institute of Immunology and Physiology, Russian Academy of Sciences, Pervomayskaya Str. 106, 620078 Yekaterinburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1424-2479","authenticated-orcid":false,"given":"Raisa","family":"Simonova","sequence":"additional","affiliation":[{"name":"Institute of Immunology and Physiology, Russian Academy of Sciences, Pervomayskaya Str. 106, 620078 Yekaterinburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-6418-8980","authenticated-orcid":false,"given":"Yulia","family":"Antonets","sequence":"additional","affiliation":[{"name":"Institute of Immunology and Physiology, Russian Academy of Sciences, Pervomayskaya Str. 106, 620078 Yekaterinburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8357-2650","authenticated-orcid":false,"given":"Anna","family":"Leiberova","sequence":"additional","affiliation":[{"name":"Institute of Immunology and Physiology, Russian Academy of Sciences, Pervomayskaya Str. 106, 620078 Yekaterinburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5583-5342","authenticated-orcid":false,"given":"Anastasia","family":"Kochurova","sequence":"additional","affiliation":[{"name":"Institute of Immunology and Physiology, Russian Academy of Sciences, Pervomayskaya Str. 106, 620078 Yekaterinburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-8483-0265","authenticated-orcid":false,"given":"Evgeniya","family":"Gusarova","sequence":"additional","affiliation":[{"name":"Institute of Immunology and Physiology, Russian Academy of Sciences, Pervomayskaya Str. 106, 620078 Yekaterinburg, Russia"},{"name":"Regional Perinatal Center, Vorovskogo Str. 70, 454052 Chelyabinsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7965-2364","authenticated-orcid":false,"given":"Tatiana","family":"Chumarnaya","sequence":"additional","affiliation":[{"name":"Institute of Immunology and Physiology, Russian Academy of Sciences, Pervomayskaya Str. 106, 620078 Yekaterinburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2976-2108","authenticated-orcid":false,"given":"Galina","family":"Kopylova","sequence":"additional","affiliation":[{"name":"Institute of Immunology and Physiology, Russian Academy of Sciences, Pervomayskaya Str. 106, 620078 Yekaterinburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6660-4673","authenticated-orcid":false,"given":"Daniil","family":"Shchepkin","sequence":"additional","affiliation":[{"name":"Institute of Immunology and Physiology, Russian Academy of Sciences, Pervomayskaya Str. 106, 620078 Yekaterinburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,2,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"109924","DOI":"10.1016\/j.diabres.2022.109924","article-title":"Geographic distribution of metabolic syndrome and its components in the general adult population: A meta-analysis of global data from 28 million individuals","volume":"188","author":"Noubiap","year":"2022","journal-title":"Diabetes Res. Clin. Pract."},{"key":"ref_2","first-page":"536","article-title":"Obesity and the Western Diet: How We Got Here","volume":"117","author":"Rakhra","year":"2020","journal-title":"Mo. Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"100375","DOI":"10.1016\/j.metop.2025.100375","article-title":"Obesity: Prevalence, causes, consequences, management, preventive strategies and future research directions","volume":"27","author":"Ahmed","year":"2025","journal-title":"Metab. Open"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"e3502","DOI":"10.1002\/dmrr.3502","article-title":"Metabolic syndrome and cardiovascular diseases: Going beyond traditional risk factors","volume":"38","author":"Barbalho","year":"2022","journal-title":"Diabetes\/Metab. Res. Rev."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1093\/eurheartj\/ehaa612","article-title":"2020 ESC Guidelines for the diagnosis and management of atrial fibrillation","volume":"42","author":"Hindricks","year":"2021","journal-title":"Eur. Heart J."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"100025","DOI":"10.1016\/j.medntd.2019.100025","article-title":"Gender differences in cardiovascular disease","volume":"4","author":"Gao","year":"2019","journal-title":"Med. Nov. Technol. Devices"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1129","DOI":"10.1016\/j.jacc.2016.11.081","article-title":"Relationship between physical activity, body mass index, and risk of heart failure","volume":"69","author":"Pandey","year":"2017","journal-title":"J. Am. Coll. Cardiol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"19154","DOI":"10.1038\/s41598-019-55529-5","article-title":"How dietary patterns affect left ventricular structure, function and remodelling","volume":"9","author":"Maugeri","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"422","DOI":"10.1016\/j.jacbts.2022.10.009","article-title":"Western diet causes heart failure with reduced ejection fraction","volume":"8","author":"Maurya","year":"2023","journal-title":"JACC Basic Transl. Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1186\/s13062-023-00363-z","article-title":"Long-term administration of Western diet induced metabolic syndrome and cardiac microvascular dysfunction","volume":"18","author":"Liu","year":"2023","journal-title":"Biol. Direct"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"E323","DOI":"10.1152\/ajpendo.00024.2008","article-title":"Cardiac inflammation associated with a Western diet is mediated via activation of RAGE by AGEs","volume":"295","author":"Tikellis","year":"2008","journal-title":"Am. J. Physiol.-Endocrinol. Metab."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"2045894018817741","DOI":"10.1177\/2045894018817741","article-title":"Adverse physiologic effects of Western diet on right ventricular structure and function","volume":"9","author":"Brittain","year":"2019","journal-title":"Pulm. Circ."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Ferron, A.J.T., Francisqueti, F.V., Minatel, I.O., Silva, C.C.V.d.A., Bazan, S.G.Z., Kitawara, K.A.H., Garcia, J.L., Corr\u00eaa, C.R., Moreto, F., and Ferreira, A.L.A. (2018). Association between cardiac remodeling and metabolic alteration in obesity induced by Western diet. Nutrients, 10.","DOI":"10.3390\/nu10111675"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"454","DOI":"10.1186\/s12933-024-02565-9","article-title":"Evaluating sex-specific responses to Western diet across the lifespan: Impact on cardiac function and transcriptomic signatures in C57BL\/6J mice at 530 and 640\/750 days of age","volume":"23","author":"Stepanyan","year":"2024","journal-title":"Cardiovasc. Diabetol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1386","DOI":"10.1002\/ehf2.15152","article-title":"Semaglutide normalizes increased cardiomyocyte calcium transients in a rat model of high fat diet-induced obesity","volume":"12","author":"Sequeira","year":"2025","journal-title":"ESC Heart Fail."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"6705","DOI":"10.1113\/JP286437","article-title":"Mechanical loading reveals intrinsic cardiomyocyte stiffness in cardiometabolic disease","volume":"602","author":"Janssens","year":"2024","journal-title":"J. Physiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"965","DOI":"10.1161\/CIRCULATIONAHA.120.052414","article-title":"Reduced right ventricular sarcomere contractility in HFpEF and obesity","volume":"143","author":"Aslam","year":"2021","journal-title":"Circulation"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"H1193","DOI":"10.1152\/ajpheart.00903.2024","article-title":"Structural and functional adaptations of human cardiomyocytes in metabolic disease and heart failure","volume":"328","author":"Huettemeister","year":"2025","journal-title":"Am. J. Physiol.-Heart Circ. Physiol."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Wen, Y., Dei\u00dfler, P.M., Prime\u00dfnig, U., Dushe, S., Falk, V., Parwani, A.S., Boldt, L.-H., Blaschke, F., Knosalla, C., and Grubitzsch, H. (2021). Impaired relaxation and reduced lusitropic reserve in atrial myocardium in obese patients. Front. Cardiovasc. Med., 8.","DOI":"10.3389\/fcvm.2021.739907"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.yjmcc.2017.12.012","article-title":"Cellular mechanisms of metabolic-syndrome-related atrial decompensation in a rat model of HFpEF","volume":"115","author":"Hohendanner","year":"2018","journal-title":"J. Mol. Cell. Cardiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"h7013","DOI":"10.1136\/bmj.h7013","article-title":"Atrial fibrillation as risk factor for cardiovascular disease and death in women compared with men: Systematic review and meta-analysis of cohort studies","volume":"532","author":"Emdin","year":"2016","journal-title":"BMJ"},{"key":"ref_22","first-page":"254","article-title":"Differential sex-dependent susceptibility to diastolic dysfunction and arrhythmia in cardiomyocytes from obese diabetic heart failure with preserved ejection fraction model","volume":"121","author":"Shen","year":"2024","journal-title":"Cardiovasc. Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1038\/ijo.2017.225","article-title":"A new animal diet based on human Western diet is a robust diet-induced obesity model: Comparison to high-fat and cafeteria diets in term of metabolic and gut microbiota disruption","volume":"42","author":"Bortolin","year":"2018","journal-title":"Int. J. Obes."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"13","DOI":"10.46235\/1028-7221-16992-SOA","article-title":"Suitability of a new diet-induced model of metabolic syndrome","volume":"28","author":"Mukhlynina","year":"2025","journal-title":"Russ. J. Immunol."},{"key":"ref_25","first-page":"2760","article-title":"Evaluation of prediabetes patients","volume":"28","author":"Genc","year":"2024","journal-title":"Eur. Rev. Med. Pharmacol. Sci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1042\/BJ20070392","article-title":"Western diet causes derangements of fatty acid metabolism and contractile dysfunction in Wistar rats","volume":"406","author":"Wilson","year":"2007","journal-title":"Biochem. J."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"2818","DOI":"10.1161\/CIRCULATIONAHA.108.832915","article-title":"Increased glucose uptake prevents FA oxidation but causes dysfunction in obesity","volume":"119","author":"Yan","year":"2009","journal-title":"Circulation"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"e008296","DOI":"10.1161\/CIRCEP.120.008296","article-title":"Ion channel and structural remodeling in obesity-mediated atrial fibrillation","volume":"13","author":"McCauley","year":"2020","journal-title":"Circ. Arrhythmia Electrophysiol."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Vileigas, D.F., Marciano, C.L.d.C., Mota, G.A.F., de Souza, S.L.B., Sant\u2019ana, P.G., Okoshi, K., Padovani, C.R., and Cicogna, A.C. (2020). Temporal Measures in Cardiac Structure and Function During the Development of Obesity Induced by Different Types of Western Diet in a Rat Model. Nutrients, 12.","DOI":"10.3390\/nu12010068"},{"key":"ref_30","first-page":"423","article-title":"Cardiac remodeling in diet-induced obesity","volume":"26","author":"Leopoldo","year":"2010","journal-title":"Can. J. Cardiol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"H655","DOI":"10.1152\/ajpheart.00684.2015","article-title":"Early cardiac changes induced by Western-type diet","volume":"310","author":"Silva","year":"2016","journal-title":"Am. J. Physiol.-Heart Circ. Physiol."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Ponce-Balbuena, D., Tyrrell, D.J., Cruz-Cort\u00e9s, C., Guerrero-Serna, G., Monteiro Da Rocha, A., Herron, T.J., Song, J., Raza, D.S., Anumonwo, J., and Goldstein, D.R. (2025). Paradoxical SERCA2a Dysregulation Contributes to Atrial Fibrillation in a Model of Diet-Induced Obesity. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms26125603"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.hlc.2007.04.006","article-title":"Body mass index is an independent determinant of left atrial size","volume":"17","author":"Ayer","year":"2008","journal-title":"Heart Lung Circ."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1982","DOI":"10.1016\/j.jacc.2009.07.034","article-title":"The aging process of the heart: Obesity is the main risk factor for left atrial enlargement during aging the MONICA\/KORA (monitoring of trends and determinations in cardiovascular disease\/cooperative research in the region of Augsburg) study","volume":"54","author":"Stritzke","year":"2009","journal-title":"J. Am. Coll. Cardiol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.carpath.2017.06.003","article-title":"Left atrial remodeling, hypertrophy, and fibrosis in mouse models of heart failure","volume":"30","author":"Hanif","year":"2017","journal-title":"Cardiovasc. Pathol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jacc.2015.04.058","article-title":"Electrophysiological, Electroanatomical, and Structural Remodeling of the Atria as Consequences of Sustained Obesity","volume":"66","author":"Mahajan","year":"2015","journal-title":"J. Am. Coll. Cardiol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"866","DOI":"10.1016\/j.yjmcc.2009.11.014","article-title":"Phosphorylation and function of cMyBP-C","volume":"48","author":"Barefield","year":"2009","journal-title":"J. Mol. Cell. Cardiol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1007\/s10974-012-9312-y","article-title":"Myosin-binding protein-C phosphorylation modulates actomyosin function in a graded manner","volume":"33","author":"Weith","year":"2012","journal-title":"J. Muscle Res. Cell Motil."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.pbiomolbio.2020.02.008","article-title":"Cardiomyocyte calcium handling in health and disease","volume":"157","author":"Sutanto","year":"2020","journal-title":"Prog. Biophys. Mol. Biol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1969","DOI":"10.1161\/CIRCRESAHA.117.310083","article-title":"Calcium signaling and cardiac arrhythmias","volume":"120","author":"Landstrom","year":"2017","journal-title":"Circ. Res."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"e13378","DOI":"10.1111\/apha.13378","article-title":"Disturbed cardiac mitochondrial and cytosolic calcium handling in a metabolic risk-related rat model of heart failure with preserved ejection fraction","volume":"228","author":"Kuster","year":"2020","journal-title":"Acta Physiol."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Kiess, T.-O., and Kocksk\u00e4mper, J. (2019). SERCA activity controls systolic nuclear calcium. Front. Physiol., 10.","DOI":"10.3389\/fphys.2019.00056"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1016\/S0006-2952(02)01625-8","article-title":"Sarcoplasmic reticulum Ca2+ uptake in LV hypertrophy","volume":"65","author":"Gupta","year":"2003","journal-title":"Biochem. Pharmacol."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Krstic, A.M., Power, A.S., and Ward, M.-L. (2023). Increased mitochondrial calcium fluxes in RV hypertrophy. Life, 13.","DOI":"10.3390\/life13020540"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"859","DOI":"10.1111\/j.1540-8167.2010.01718.x","article-title":"Comparative expression of proteins in left and right atrial appendages from patients with mitral valve disease at sinus rhythm and atrial fibrillation","volume":"21","author":"Modrego","year":"2010","journal-title":"J. Cardiovasc. Electrophysiol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1161\/CIRCEP.110.954636","article-title":"Left-to-right atrial inward rectifier potassium current gradients in patients with paroxysmal versus chronic atrial fibrillation","volume":"3","author":"Voigt","year":"2010","journal-title":"Circ. Arrhythm. Electrophysiol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1415","DOI":"10.1016\/j.hrthm.2009.06.018","article-title":"Left versus right atrial difference in dominant frequency, K+ channel transcripts, and fibrosis in patients developing atrial fibrillation after cardiac surgery","volume":"6","author":"Swartz","year":"2009","journal-title":"Heart Rhythm"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1161\/CIRCGENETICS.111.961631","article-title":"Whole genome expression differences in human left and right atria ascertained by RNA sequencing","volume":"5","author":"Hsu","year":"2012","journal-title":"Circ. Cardiovasc. Genet."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/j.ijcard.2016.09.061","article-title":"Review: The outcomes of different vessel diameter in patients receiving coronary artery stenting","volume":"224","author":"Tsai","year":"2016","journal-title":"Int. J. Cardiol."},{"key":"ref_50","first-page":"242","article-title":"Transcriptomic Landscape of Human Left and Right Atria Associated with Atrial Fibrillation","volume":"29","year":"2025","journal-title":"Anatol. J. Cardiol."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Kim, J.C., Huynh, H.T., Luong, P.K., Trinh, T.N., Wu, Y., Grandi, E., and Woo, S.H. (2026). Mechanisms underlying local Ca2+ signalling differences between right and left atrial myocytes at normal and increased frequencies. J. Physiol., early view.","DOI":"10.1113\/JP287884"},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Butova, X., Myachina, T., Simonova, R., Kochurova, A., Mukhlynina, E., Kopylova, G., Shchepkin, D., and Khokhlova, A. (2023). The inter-chamber differences in the contractile function between left and right atrial cardiomyocytes in atrial fibrillation in rats. Front. Cardiovasc. Med., 10.","DOI":"10.3389\/fcvm.2023.1203093"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"11765","DOI":"10.1073\/pnas.201394198","article-title":"Estrogen receptors activate atrial natriuretic peptide in the rat heart","volume":"98","author":"Jankowski","year":"2001","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Myachina, T.A., Butova, X.A., Simonova, R.A., Volzhaninov, D.A., Kochurova, A.M., Kopylova, G.V., Shchepkin, D.V., and Khokhlova, A.D. (2025). The Contractile Function of Ventricular Cardiomyocytes Is More Sensitive to Acute 17\u03b2-Estradiol Treatment Compared to Atrial Cardiomyocytes. Cells, 14.","DOI":"10.3390\/cells14080561"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.bbrc.2020.12.102","article-title":"Differing effects of estrogen deficiency on the contractile function of atrial and ventricular myocardium","volume":"541","author":"Khokhlova","year":"2021","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"3130","DOI":"10.1002\/ehf2.13419","article-title":"Right-ventricular dysfunction in HFpEF is linked to altered cardiomyocyte Ca2+ homeostasis and myofilament sensitivity","volume":"8","author":"Hegemann","year":"2021","journal-title":"ESC Heart Fail."},{"key":"ref_57","first-page":"100075","article-title":"Roles of cMyBP-C phosphorylation on cardiac contractile dysfunction in db\/db mice","volume":"8","author":"Desai","year":"2024","journal-title":"J. Mol. Cell. Cardiol. Plus"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"e15279","DOI":"10.14814\/phy2.15279","article-title":"Intermittent fasting and exercise therapy abates STZ-induced diabetotoxicity in rats through modulation of adipocytokines hormone, oxidative glucose metabolic, and glycolytic pathway","volume":"10","author":"Chijiokwu","year":"2022","journal-title":"Physiol. Rep."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1093\/clinchem\/36.1.15","article-title":"Estimating LDL cholesterol by Friedewald equation","volume":"36","author":"Warnick","year":"1990","journal-title":"Clin. Chem."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"101189","DOI":"10.1016\/j.mex.2020.101189","article-title":"A combined Langendorff-injection technique for isolation of cardiomyocytes","volume":"8","author":"Butova","year":"2021","journal-title":"MethodsX"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"732","DOI":"10.1134\/S0006350919050178","article-title":"Development and Program Implementation of an Algorithm to Estimate the Mean Sarcomere Length of a Cardiomyocyte","volume":"64","author":"Myachina","year":"2019","journal-title":"Biophysics"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/0076-6879(82)85009-X","article-title":"Preparation of myosin and its subfragments","volume":"85","author":"Margossian","year":"1982","journal-title":"Methods Enzymol."},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Butova, X., Myachina, T., Simonova, R., Kochurova, A., Bozhko, Y., Arkhipov, M., Solovyova, O., Kopylova, G., Shchepkin, D., and Khokhlova, A. (2022). Peculiarities of the Acetylcholine Action on the Contractile Function of Cardiomyocytes from the Left and Right Atria in Rats. Cells, 11.","DOI":"10.3390\/cells11233809"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1021\/acs.biochem.6b00994","article-title":"Structural and functional effects of cardiomyopathy-causing mutations in the Troponin T-Binding Region of Cardiac Tropomyosin","volume":"56","author":"Matyushenko","year":"2017","journal-title":"Biochemistry"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"2199","DOI":"10.1529\/biophysj.106.081117","article-title":"Automatic detection of single fluorophores in Live Cells","volume":"92","author":"Mashanov","year":"2007","journal-title":"Biophys. J."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/3\/1508\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T16:47:23Z","timestamp":1770137243000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/3\/1508"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,3]]},"references-count":65,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2026,2]]}},"alternative-id":["ijms27031508"],"URL":"https:\/\/doi.org\/10.3390\/ijms27031508","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,3]]}}}