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Recently, it has been shown that these channels contribute to the release of glutamate from astrocytes. Since enhanced extracellular glutamate concentrations produce hyperexcitability, and microdialysis revealed elevated levels of the transmitter in the brains of epileptic patients, we asked whether astroglial glutamate release through LRRC8\/VRACs might contribute to the initiation of experimental temporal lobe epilepsy (TLE). Patch clamp, pharmacological, and single-cell transcript analyses were performed in the hippocampus of controls and mice with inducible deletion of LRRC8a in astrocytes. In addition, these mice were exposed to our unilateral intracortical kainate model of TLE. Tonic currents were recorded from CA1 pyramidal neurons as a measure of glutamate release. Our data show that neither expression of LRRC8a nor the amplitude of tonic currents was altered 4 h after status epilepticus-induced TLE. These findings do not suggest that increased astroglial glutamate release through LRRC8 channels contributes to the initiation of experimental TLE.<\/jats:p>","DOI":"10.3390\/ijms27031589","type":"journal-article","created":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T17:09:41Z","timestamp":1770311381000},"page":"1589","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["LRRC8-Mediated Glutamate Release from Astrocytes Is Not Increased During the Initiation of Experimental Temporal Lobe Epilepsy"],"prefix":"10.3390","volume":"27","author":[{"given":"Kamyab","family":"Sarmadi","sequence":"first","affiliation":[{"name":"Institute of Cellular Neurosciences I, University Hospital Bonn, University of Bonn, 53127 Bonn, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda","family":"Gaspar","sequence":"additional","affiliation":[{"name":"Institute of Cellular Neurosciences I, University Hospital Bonn, University of Bonn, 53127 Bonn, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0090-7553","authenticated-orcid":false,"given":"Peter","family":"Bedner","sequence":"additional","affiliation":[{"name":"Institute of Cellular Neurosciences I, University Hospital Bonn, University of Bonn, 53127 Bonn, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4749-5162","authenticated-orcid":false,"given":"Lukas","family":"Henning","sequence":"additional","affiliation":[{"name":"Institute of Cellular Neurosciences I, University Hospital Bonn, University of Bonn, 53127 Bonn, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5391-7387","authenticated-orcid":false,"given":"Christian","family":"Henneberger","sequence":"additional","affiliation":[{"name":"Institute of Cellular Neurosciences I, University Hospital Bonn, University of Bonn, 53127 Bonn, Germany"},{"name":"German Center for Neurodegenerative Diseases (DZNE), 53127 Bonn, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6612-7678","authenticated-orcid":false,"given":"Ronald","family":"Jabs","sequence":"additional","affiliation":[{"name":"Institute of Cellular Neurosciences I, University Hospital Bonn, University of Bonn, 53127 Bonn, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3509-2553","authenticated-orcid":false,"given":"Thomas J.","family":"Jentsch","sequence":"additional","affiliation":[{"name":"Leibniz-Forschungsinstitut f\u00fcr Molekulare Pharmakologie (FMP), 13125 Berlin, Germany"},{"name":"NeuroCure Cluster of Excellence, Charit\u00e9 Universit\u00e4tsmedizin Berlin, 10117 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2579-8357","authenticated-orcid":false,"given":"Christian","family":"Steinh\u00e4user","sequence":"additional","affiliation":[{"name":"Institute of Cellular Neurosciences I, University Hospital Bonn, University of Bonn, 53127 Bonn, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerald","family":"Seifert","sequence":"additional","affiliation":[{"name":"Institute of Cellular Neurosciences I, University Hospital Bonn, University of Bonn, 53127 Bonn, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,2,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1002\/glia.20174","article-title":"Astrocytic Swelling in Cerebral Ischemia as a Possible Cause of Injury and Target for Therapy","volume":"50","author":"Kimelberg","year":"2005","journal-title":"Glia"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1038\/nrm.2016.29","article-title":"VRACs and Other Ion Channels and Transporters in the Regulation of Cell Volume and Beyond","volume":"17","author":"Jentsch","year":"2016","journal-title":"Nat. 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