{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T06:19:01Z","timestamp":1770963541579,"version":"3.50.1"},"reference-count":49,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T00:00:00Z","timestamp":1770768000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004663","name":"National Science and Technology Council","doi-asserted-by":"publisher","award":["NSTC114-2320-B-039-035-MY3"],"award-info":[{"award-number":["NSTC114-2320-B-039-035-MY3"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004663","name":"National Science and Technology Council","doi-asserted-by":"publisher","award":["NSTC114-2320-B-039-036-MY3"],"award-info":[{"award-number":["NSTC114-2320-B-039-036-MY3"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007300","name":"China Medical University","doi-asserted-by":"crossref","award":["CMU113-MF-51"],"award-info":[{"award-number":["CMU113-MF-51"]}],"id":[{"id":"10.13039\/501100007300","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100007300","name":"China Medical University","doi-asserted-by":"crossref","award":["CMU114-MF-30"],"award-info":[{"award-number":["CMU114-MF-30"]}],"id":[{"id":"10.13039\/501100007300","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100007300","name":"China Medical University","doi-asserted-by":"crossref","award":["CMU114-MF-31"],"award-info":[{"award-number":["CMU114-MF-31"]}],"id":[{"id":"10.13039\/501100007300","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Chronic inflammation-driven colorectal cancer (CRC) is critically mediated by interleukin-17A (IL-17A)-dependent immune responses and nuclear factor-\u03baB (NF-\u03baB) signaling, which promote immune cell infiltration and tumor progression. In this study, the anti-tumor efficacy and molecular mechanisms of a standardized extract of Hedyotis diffusa Willd. (HD) and its constituent, ferulic acid (FA), were investigated using an azoxymethane\/dextran sulfate sodium (AOM\/DSS)-induced colitis-associated CRC mouse model. HD and FA treatment markedly alleviated colitis, reduced tumor number and size, improved survival, and attenuated histopathological damage. Transcriptomic analysis revealed significant modulation of immune-related pathways, with prominent suppression of IL-17A and NF-\u03baB signaling. Molecular docking demonstrated binding of FA to IL-17A at Pro59 and Arg101, suggesting potential disruption of the IL-17A\/IL-17RA interaction. Consistently, both HD and FA reduced immune cell infiltration, downregulated IL-17A production, and inhibited NF-\u03baB activation in colonic tissues. Collectively, these findings demonstrated that HD exerted protective effects against inflammation-associated CRC through targeting the IL-17A\/IL-17RA axis and downstream NF-\u03baB signaling, providing mechanistic insight into IL-17A-centered immunomodulation in colorectal tumorigenesis.<\/jats:p>","DOI":"10.3390\/ijms27041745","type":"journal-article","created":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T17:45:36Z","timestamp":1770831936000},"page":"1745","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Hedyotis diffusa Suppresses Colitis-Associated Colorectal Cancer via Inhibition of the IL-17A-IL-17RA Axis and NF-\u03baB Signaling"],"prefix":"10.3390","volume":"27","author":[{"given":"Yun-Jhu","family":"Hou","sequence":"first","affiliation":[{"name":"Department of Veterinary Medicine, School of Veterinary Medicine, National Taiwan University, Taipei 106319, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6507-8015","authenticated-orcid":false,"given":"Chien-Yun","family":"Hsiang","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, China Medical University, Taichung 404328, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hsin-Yi","family":"Lo","sequence":"additional","affiliation":[{"name":"School of Chinese Medicine & Graduate Institute of Chinese Medicine, China Medical University, Taichung 404328, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0271-5416","authenticated-orcid":false,"given":"Fang-Chia","family":"Chang","sequence":"additional","affiliation":[{"name":"Graduate Institute of Veterinary Medicine, National Taiwan University, Taipei 106319, Taiwan"},{"name":"Neurobiology and Cognitive Science Center, National Taiwan University, Taipei 106319, Taiwan"},{"name":"Graduate Institute of Brain and Mind Science, National Taiwan University, Taipei 106319, Taiwan"},{"name":"Graduate Institute of Acupuncture Science, China Medical University, Taichung 404328, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2390-1461","authenticated-orcid":false,"given":"Tin-Yun","family":"Ho","sequence":"additional","affiliation":[{"name":"School of Chinese Medicine & Graduate Institute of Chinese Medicine, China Medical University, Taichung 404328, Taiwan"},{"name":"Department of Health and Nutrition Biotechnology, Asia University, Taichung 413305, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,2,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Basile, D., Di Nardo, P., Daffin\u00e0, M.G., Cortese, C., Giuliani, J., and Aprile, G. 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