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Recent angiogenesis-focused data showed that prenatal alcohol exposure (PAE) disrupts inter-organ gene expression between the placenta and fetal cortex. The present study aimed to perform the first comprehensive and untargeted analysis of a murine placenta\u2013cortex transcriptomic database of PAE. Gene lists from a recently NCBI-deposited PAE Placenta\u2013Cortex transcriptomic database were analyzed using g:Profiler for unbiased functional profiling querying Gene Ontology, KEGG, and Reactome databases. Genes intersecting with cell\u2013cell communication terms were submitted to STRING and ShinyGO analyses to identify enriched protein\u2013protein interactions and pathways. Several ligand or receptor candidates were then validated by Western blot. g:Profiler revealed 21 enriched GO functional maps, seven KEGG pathways, and six Reactome pathways, of which 11 were related to cell-to-cell communication. STRING analysis exhibited substantial protein\u2013protein interaction enrichments supporting that proteins belonging to the functional maps and pathways are biologically connected. Notably, 38 ligands or receptors from endocrine families including angiotensinogen, leptin, somatostatin, or PACAP were identified. Western blot analysis of protein candidates showed different validation patterns. In particular, the PACAP receptor family confirmed transcriptomic findings and revealed sex-dependent PAE-impacted expression profiles. The present study indicates that PAE is associated with alterations in the transcriptomic placenta\u2013cortex expression profile, including changes in the expression ratios of several ligands and\/or receptors implicated in key physiological pathways such as energy balance, vascular development, and neurogenesis. These transcriptomic associations suggest that altered placenta\u2013fetal brain signaling at the gene expression level may be involved in alcohol-induced neurodevelopmental disorders, highlighting the need for future functional validation studies.<\/jats:p>","DOI":"10.3390\/ijms27041819","type":"journal-article","created":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T10:14:32Z","timestamp":1771236872000},"page":"1819","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Comprehensive Analysis of the Placenta\u2013Cortex Transcriptomic Database Reveals a Neuroactive Ligand\u2013Receptor Dysregulation After Prenatal Alcohol Exposure"],"prefix":"10.3390","volume":"27","author":[{"given":"Camille","family":"Sautreuil","sequence":"first","affiliation":[{"name":"University Rouen Normandie, INSERM U1245, Team Epigenetics and Pathophysiology of Neurodevelopmental Disorders, 76183 Rouen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1589-4097","authenticated-orcid":false,"given":"Maryline","family":"Lecointre","sequence":"additional","affiliation":[{"name":"University Rouen Normandie, INSERM U1245, Team Epigenetics and Pathophysiology of Neurodevelopmental Disorders, 76183 Rouen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6722-5955","authenticated-orcid":false,"given":"C\u00e9line","family":"Derambure","sequence":"additional","affiliation":[{"name":"University Rouen Normandie, INSERM U1245, Team Genetic Predisposition to Cancer, 76183 Rouen, France"},{"name":"Joint Genomics Facilities, Rouen University, 76183 Rouen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4370-2865","authenticated-orcid":false,"given":"Carole","family":"Brasse-Lagnel","sequence":"additional","affiliation":[{"name":"University Rouen Normandie, INSERM U1245, Team Epigenetics and Pathophysiology of Neurodevelopmental Disorders, 76183 Rouen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9391-7800","authenticated-orcid":false,"given":"Ga\u00ebl","family":"Nicolas","sequence":"additional","affiliation":[{"name":"University Rouen Normandie, INSERM U1245, Team Genomics for Brain Disorders, 76183 Rouen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie","family":"Gil","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Cit\u00e9, INSERM UMR-S 1144, Optimisation Th\u00e9rapeutique en Neuropsychopharmacologie, OTeN, F-75006 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel D.","family":"Savage","sequence":"additional","affiliation":[{"name":"Department of Neurosciences, School of Medicine, University of New Mexico, Albuquerque, NM 87106, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2858-148X","authenticated-orcid":false,"given":"St\u00e9phane","family":"Marret","sequence":"additional","affiliation":[{"name":"University Rouen Normandie, INSERM U1245, Team Epigenetics and Pathophysiology of Neurodevelopmental Disorders, 76183 Rouen, France"},{"name":"Department of Neonatal Paediatrics and Intensive Care, Rouen University Hospital and University of Rouen Normandie, 76000 Rouen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7306-0520","authenticated-orcid":false,"given":"Florent","family":"Marguet","sequence":"additional","affiliation":[{"name":"University Rouen Normandie, INSERM U1245, Team Epigenetics and Pathophysiology of Neurodevelopmental Disorders, 76183 Rouen, France"},{"name":"Department of Pathology, Rouen University Hospital, 76000 Rouen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7176-1320","authenticated-orcid":false,"given":"Bruno J.","family":"Gonzalez","sequence":"additional","affiliation":[{"name":"University Rouen Normandie, INSERM U1245, Team Epigenetics and Pathophysiology of Neurodevelopmental Disorders, 76183 Rouen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4081-6704","authenticated-orcid":false,"given":"Anthony","family":"Falluel-Morel","sequence":"additional","affiliation":[{"name":"University Rouen Normandie, INSERM U1245, Team Epigenetics and Pathophysiology of Neurodevelopmental Disorders, 76183 Rouen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,2,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1663","DOI":"10.1098\/rstb.2014.0066","article-title":"The placenta: A multifaceted, transient organ","volume":"370","author":"Burton","year":"2015","journal-title":"Philos. Trans. R. Soc. Lond. B Biol. Sci."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Ahmadi, S.M., Perez, M.L., and Guardia, C.M. (2025). Secretion of placental peptide hormones: Functions and trafficking. Front. Endocrinol., 16.","DOI":"10.3389\/fendo.2025.1584303"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"dev202088","DOI":"10.1242\/dev.202088","article-title":"Maternal-fetal cross-talk via the placenta: Influence on offspring development and metabolism","volume":"150","author":"Kramer","year":"2023","journal-title":"Development"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Bakalar, D., O\u2019Reilly, J.J., Lacaille, H., Salzbank, J., Ellegood, J., Lerch, J.P., Sasaki, T., Imamura, Y., Hashimoto-Torii, K., and Vacher, C.M. (2022). Lack of placental neurosteroid alters cortical development and female somatosensory function. Front. Endocrinol., 13.","DOI":"10.3389\/fendo.2022.972033"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Sautreuil, C., Lecointre, M., Dalmasso, J., Lebon, A., Leuillier, M., Janin, F., Lecuyer, M., Bekri, S., Marret, S., and Laquerri\u00e8re, A. (2024). Expression of placental CD146 is dysregulated by prenatal alcohol exposure and contributes in cortical vasculature development and positioning of vessel-associated oligodendrocytes. Front. Cell. Neurosci., 17.","DOI":"10.3389\/fncel.2023.1294746"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1186\/s40478-017-0444-6","article-title":"PLGF, a placental marker of fetal brain defects after in utero alcohol exposure","volume":"5","author":"Lecuyer","year":"2017","journal-title":"Acta Neuropathol. Commun."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Sautreuil, C., Lecointre, M., Derambure, C., Brasse-Lagnel, C., Leroux, P., Laquerri\u00e8re, A., Nicolas, G., Gil, S., Savage, D.D., and Marret, S. (2023). Prenatal alcohol exposure impairs the placenta-cortex transcriptomic signature, leading to dysregulation of angiogenic pathways. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms241713484"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"115453","DOI":"10.1016\/j.expneurol.2025.115453","article-title":"Placental Igf1 overexpression sex-specifically Impacts Mouse Placenta Structure, Altering Offspring Striatal Development and Behavior","volume":"394","author":"Carver","year":"2025","journal-title":"Exp. Neurol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"e13489","DOI":"10.1111\/jne.13489","article-title":"Neuroactive steroid exposure impacts neurodevelopment: Comparison of human and rodent placental contribution","volume":"37","author":"Vacher","year":"2025","journal-title":"J. Neuroendocrinol."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Tsompanidis, A., Warrier, V., and Baron-Cohen, S. (2023). The genetics of autism and steroid-related traits in prenatal and postnatal life. Front. Endocrinol., 14.","DOI":"10.3389\/fendo.2023.1126036"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1392","DOI":"10.1038\/s41593-021-00896-4","article-title":"Placental endocrine function shapes cerebellar development and social behavior","volume":"24","author":"Vacher","year":"2021","journal-title":"Nat. Neurosci."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Ayash, T.A., Allard, M.J., Chevin, M., and S\u00e9bire, G. (2024). IL-1 blockade mitigates autism and cerebral palsy traits in offspring in-utero exposed to group B streptococcus chorioamnionitis. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms252111393"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Patel, P., Ku, J., Uzoaru, I., and Goldstein, J.A. (2025). Placental biomarkers for the prediction of neurodevelopmental disorders. Front. Cell Dev. Biol., 13.","DOI":"10.3389\/fcell.2025.1663960"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1038\/s41572-023-00420-x","article-title":"Fetal alcohol spectrum disorders","volume":"9","author":"Popova","year":"2023","journal-title":"Nat. Rev. Dis. Primers"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"952","DOI":"10.1002\/ana.23699","article-title":"Prenatal alcohol exposure affects vasculature development in the neonatal brain","volume":"72","author":"Marret","year":"2012","journal-title":"Ann. Neurol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"e186096","DOI":"10.1172\/jci.insight.186096","article-title":"Prenatal alcohol exposure is associated with altered feto-placental blood flow and sex-specific placental changes","volume":"10","author":"Steane","year":"2025","journal-title":"JCI Insight"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"W207","DOI":"10.1093\/nar\/gkad347","article-title":"g:Profiler-interoperable web service for functional enrichment analysis and gene identifier mapping (2023 update)","volume":"51","author":"Kolberg","year":"2023","journal-title":"Nucleic Acids Res."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Lu, J., Piper, S.J., Zhao, P., Miller, L.J., Wootten, D., and Sexton, P.M. (2022). Targeting VIP and PACAP receptor signaling: New insights into designing drugs for the PACAP subfamily of receptors. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23158069"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1359","DOI":"10.1111\/j.1530-0277.1995.tb01625.x","article-title":"Effect of pre- or postnatal exposure to ethanol on the total number of neurons in the principal sensory nucleus of the trigeminal nerve: Cell proliferation and neuronal death","volume":"19","author":"Miller","year":"1995","journal-title":"Alcohol Clin. Exp. Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1056","DOI":"10.1126\/science.287.5455.1056","article-title":"Ethanol-induced apoptotic neurodegeneration and fetal alcohol syndrome","volume":"287","author":"Ikonomidou","year":"2000","journal-title":"Science"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"105791","DOI":"10.1016\/j.nbd.2022.105791","article-title":"In utero alcohol exposure impairs vessel-associated positioning and differentiation of oligodendrocytes in the developing neocortex","volume":"171","author":"Brosolo","year":"2022","journal-title":"Neurobiol. Dis."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"150875","DOI":"10.1016\/j.bbrc.2024.150875","article-title":"Maternal alcohol consumption up to mouse organogenesis disrupts fetal-placental interface at mid-gestation associated with dysregulation of AQP3 immunoexpression","volume":"736","author":"Barril","year":"2024","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1036","DOI":"10.1111\/acer.14846","article-title":"Collaborative Initiative on Fetal Alcohol Spectrum Disorders. Prenatal alcohol exposure contributes to negative pregnancy outcomes by altering fetal vascular dynamics and the placental transcriptome","volume":"46","author":"Pinson","year":"2022","journal-title":"Alcohol Clin. Exp. Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1016\/j.alcohol.2009.10.002","article-title":"Effects of moderate drinking during pregnancy on placental gene expression","volume":"44","author":"Rosenberg","year":"2010","journal-title":"Alcohol"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"147951","DOI":"10.1016\/j.gene.2023.147951","article-title":"RNA-seq analysis reveals prenatal alcohol exposure is associated with placental inflammatory cells and gene expression","volume":"894","author":"Williams","year":"2024","journal-title":"Gene"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1159\/000547695","article-title":"sFlt-1\/PlGF ratio predicts serious outcomes in early-onset fetal growth restriction","volume":"18","author":"Shinohara","year":"2025","journal-title":"Fetal Diagn. Ther."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Mocan, D., Jipa, R., Jipa, D.A., Lala, R.I., Puschita, M., Rasinar, F.C., Balta, D.F., Groza, I.S., and Uzum, A. (2025). Soluble CD146 in heart failure: Pathophysiological role and diagnostic potential. Biomedicines, 13.","DOI":"10.20944\/preprints202504.1104.v1"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1577","DOI":"10.1016\/S0006-2952(97)00017-8","article-title":"Human placental cholinergic system","volume":"53","author":"Sastry","year":"1997","journal-title":"Biochem Pharmacol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"660","DOI":"10.1016\/j.placenta.2008.05.009","article-title":"Somatostatin as a regulator of first-trimester human trophoblast functions","volume":"29","author":"Biondi","year":"2008","journal-title":"Placenta"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1177\/0960327120954259","article-title":"Gene expression in rat placenta after exposure to di(2-ethylhexyl) phthalate","volume":"40","author":"Xu","year":"2021","journal-title":"Hum. Exp. Toxicol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1210\/jcem-56-3-567","article-title":"Fetal and maternal plasma levels of immunoreactive somatostatin at delivery: Evidence for its increase in the umbilical artery and its arterio-venous gradient in the feto-placental circulation","volume":"56","author":"Saito","year":"1983","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1038\/s41586-024-08108-2","article-title":"Leptin-activated hypothalamic BNC2 neurons acutely suppress food intake","volume":"636","author":"Tan","year":"2024","journal-title":"Nature"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Elgazzaz, M., Ogbi, S., Moronge, D., Mellott, E., Cooper, G., Backer, K., Hitchings, J., Zarate, L.V., Kavuri, S., and Lee, T.J. (2025). Vascular endothelial growth factor receptors 1 and 3 mediate placental trophoblast leptin production in preeclampsia, inducing vascular dysfunction. bioRxiv.","DOI":"10.1101\/2025.05.27.656489"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Rumer, K.K., Sehgal, S., Kramer, A., Bogart, K.P., and Winn, V.D. (2024). The effects of leptin on human cytotrophoblast invasion are gestational age and dose-dependent. Front. Endocrinol., 15.","DOI":"10.3389\/fendo.2024.1386309"},{"key":"ref_35","first-page":"645","article-title":"Leptin concentrations in the umbilical vein and artery. Relationship to maternal and neonatal anthropometry","volume":"47","author":"Yang","year":"2002","journal-title":"J. Reprod. Med."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1080\/10284150600630676","article-title":"Ontogenic expression of putative feeding peptides in the rat fetal brain and placenta","volume":"9","author":"Beloosesky","year":"2006","journal-title":"Nutr. Neurosci."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1007\/s12031-025-02366-z","article-title":"More than three decades after discovery of the neuroprotective effect of PACAP, what is still preventing its clinical use?","volume":"75","author":"Cherait","year":"2025","journal-title":"J. Mol. Neurosci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1002\/mrd.10221","article-title":"Expression patterns of pituitary adenylate cyclase activating polypeptide and its type I receptor mRNAs in the rat placenta","volume":"64","author":"Koh","year":"2003","journal-title":"Mol. Reprod. Dev."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1016\/j.earlhumdev.2014.06.001","article-title":"Pituitary adenylate cyclase-activating polypeptide (PACAP) in fetal cord blood","volume":"90","author":"Winters","year":"2014","journal-title":"Early Hum. Dev."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/S0165-3806(98)00086-8","article-title":"Expression of PACAP, and PACAP type 1 (PAC1) receptor mRNA during development of the mouse embryo","volume":"109","author":"Sheward","year":"1998","journal-title":"Brain Res. Dev. Brain Res."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1177","DOI":"10.1002\/jnr.21026","article-title":"Pituitary adenylate cyclase-activating polypeptide regulates forebrain neural stem cells and neurogenesis in vitro and in vivo","volume":"84","author":"Ohta","year":"2006","journal-title":"J. Neurosci. Res."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1007\/978-3-319-33826-2_10","article-title":"Validation of transcriptomics-based in vitro methods","volume":"856","author":"Corvi","year":"2016","journal-title":"Adv. Exp. Med. Biol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"105074","DOI":"10.1016\/j.nbd.2020.105074","article-title":"In utero alcohol exposure exacerbates endothelial protease activity from pial microvessels and impairs GABA interneuron positioning","volume":"145","author":"Lecointre","year":"2020","journal-title":"Neurobiol. Dis."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"613","DOI":"10.1111\/acer.15017","article-title":"Sex-related differences among individuals assessed for fetal alcohol spectrum disorder in Canada","volume":"47","author":"Flannigan","year":"2023","journal-title":"Alcohol Clin. Exp. Res."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Ellenberger, D.T., Lyu, Z., Herrington, R.T.B., Kinkade, J.A., Leone, G.W., Sze, J.Y., Bivens, N.J., Baker, R.F., Roberts, R.M., and Joshi, T. (2025). Disruption of the Placenta-Brain Axis in Transgenic Mice Lacking Serotonin Transporter (SERT) in Trophoblast Cells. Int. J. Mol. Sci., 27.","DOI":"10.3390\/ijms27010436"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"112790","DOI":"10.1016\/j.drugalcdep.2025.112790","article-title":"Prevalence of congenital heart defects among children with and without diagnosed fetal alcohol spectrum disorders, 2016\u20132022","volume":"274","author":"Dorsey","year":"2025","journal-title":"Drug. Alcohol Depend."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1607","DOI":"10.1093\/cvr\/cvae139","article-title":"Embryonic alcohol exposure in zebrafish predisposes adults to cardiomyopathy and diastolic dysfunction","volume":"120","author":"Weeks","year":"2024","journal-title":"Cardiovasc. Res."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1007\/s12012-018-09503-8","article-title":"Prenatal alcohol exposure causes adverse cardiac extracellular matrix changes and dysfunction in neonatal mice","volume":"19","author":"Ninh","year":"2019","journal-title":"Cardiovasc. Toxicol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.alcohol.2017.05.004","article-title":"Challenges of diagnosing fetal alcohol spectrum disorders in foster and adopted children","volume":"67","author":"Bakhireva","year":"2018","journal-title":"Alcohol"},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Celis, C., Troncoso, F., L\u00f3pez, E., Escudero-Guevara, E., Acurio, J., and Escudero, C. (2025). Dysregulated levels of proangiogenic proteins in the placentas of children with autism spectrum disorder and attention-deficit hyperactivity disorder. Front. Med., 12.","DOI":"10.3389\/fmed.2025.1693975"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"793","DOI":"10.1016\/j.pcl.2025.05.002","article-title":"Placental role in fetal neonatal neurologic outcomes","volume":"72","author":"Chalak","year":"2025","journal-title":"Pediatr. Clin. N. Am."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1093\/nar\/gkg034","article-title":"STRING: A database of predicted functional associations between proteins","volume":"31","author":"Huynen","year":"2003","journal-title":"Nucleic Acids Res."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"D638","DOI":"10.1093\/nar\/gkac1000","article-title":"The STRING database in 2023: Protein-protein association networks and functional enrich-ent analyses for any sequenced genome of interest","volume":"51","author":"Szklarczyk","year":"2023","journal-title":"Nucleic Acids Res."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"89","DOI":"10.12997\/jla.2023.12.2.89","article-title":"Are only p-values less than 0.05 significant? A p-value greater than 0.05 is also significant!","volume":"12","author":"Kwak","year":"2023","journal-title":"J. Lipid Atheroscler."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1037\/a0024338","article-title":"Effect size estimates: Current use, calculations, and interpretation","volume":"141","author":"Fritz","year":"2012","journal-title":"J. Exp. Psychol. Gen."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/4\/1819\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T05:14:14Z","timestamp":1771305254000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/4\/1819"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,14]]},"references-count":55,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2026,2]]}},"alternative-id":["ijms27041819"],"URL":"https:\/\/doi.org\/10.3390\/ijms27041819","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,14]]}}}