{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T07:28:30Z","timestamp":1772177310664,"version":"3.50.1"},"reference-count":26,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T00:00:00Z","timestamp":1771545600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100031666","name":"University of Miskolc","doi-asserted-by":"crossref","award":["ME-TKTP-2025-046"],"award-info":[{"award-number":["ME-TKTP-2025-046"]}],"id":[{"id":"10.13039\/100031666","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The rising global incidence of Type 1 Diabetes Mellitus (T1DM) necessitates a deeper understanding of the molecular shifts underlying its metabolic complications, specifically the role of protein N-glycosylation. This study utilized a streptozotocin-induced C57Bl\/6 mouse model to examine temporal changes in plasma N-glycan profiles at 2, 8, and 20 weeks post-induction using HILIC-UPLC-FLR-MS. Following the successful establishment of persistent hyperglycemia and weight loss, glycomic analysis revealed significant structural remodeling of 20 individual glycan species, with complex, multi-sialylated structures proving most sensitive to disease progression. Notably, bi-antennary structures such as A2G1S1, A2G2S1, and A2G2S2(2) exhibited a marked decrease in relative abundance that strongly correlated with elevated blood glucose levels. In contrast, highly sialylated and fucosylated glycans like FA2G2S3 and FA3G3S3 showed a progressive increase over the 20-week period, suggesting an adaptive response to chronic metabolic stress and altered hepatic processing. Our findings demonstrate that chronic hyperglycemia is accompanied by substantial remodeling of the plasma N-glycome, characterized by increased sialylation and fucosylation. These alterations closely track the progression of metabolic dysregulation, suggesting that while they parallel blood glucose trends, they provide a distinct molecular readout of the systemic glycosylation response to glucotoxicity. This study offers a detailed longitudinal characterization of these glycomic changes, highlighting their potential value as descriptive markers of cumulative metabolic stress in rodent models of type 1 diabetes.<\/jats:p>","DOI":"10.3390\/ijms27042010","type":"journal-article","created":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T10:32:37Z","timestamp":1771583557000},"page":"2010","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Longitudinal Profiling of Plasma N-Glycomic Alterations in an STZ-Induced Mouse Model of Hyperglycemia"],"prefix":"10.3390","volume":"27","author":[{"given":"Adri\u00e1na","family":"Kut\u00e1s","sequence":"first","affiliation":[{"name":"Materials and Intelligent Technologies Higher Education and Industrial Cooperation Centre, University of Miskolc, 3515 Miskolc, Hungary"},{"name":"Institute of Molecular Life Sciences, Research Centre for Natural Sciences, Hungarian Research Network, 1117 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5435-4993","authenticated-orcid":false,"given":"Viola","family":"Pomozi","sequence":"additional","affiliation":[{"name":"Institute of Molecular Life Sciences, Research Centre for Natural Sciences, Hungarian Research Network, 1117 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Krisztina","family":"F\u00fcl\u00f6p","sequence":"additional","affiliation":[{"name":"Institute of Molecular Life Sciences, Research Centre for Natural Sciences, Hungarian Research Network, 1117 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0777-9569","authenticated-orcid":false,"given":"B\u00e9la","family":"Viskolcz","sequence":"additional","affiliation":[{"name":"Materials and Intelligent Technologies Higher Education and Industrial Cooperation Centre, University of Miskolc, 3515 Miskolc, Hungary"},{"name":"Institute of Chemistry, Faculty of Materials Science and Chemical Engineering, University of Miskolc, 3515 Miskolc, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Attila","family":"Garami","sequence":"additional","affiliation":[{"name":"Institute of Energy, Ceramic and Polymer Technology, University of Miskolc, 3515 Miskolc, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2672-095X","authenticated-orcid":false,"given":"Csaba","family":"V\u00e1radi","sequence":"additional","affiliation":[{"name":"Institute of Chemistry, Faculty of Materials Science and Chemical Engineering, University of Miskolc, 3515 Miskolc, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,2,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/j.diabres.2013.11.005","article-title":"Diabetes in the young\u2014A global view and worldwide estimates of numbers of children with type 1 diabetes","volume":"103","author":"Patterson","year":"2014","journal-title":"Diabetes Res. 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