{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T13:05:23Z","timestamp":1771938323079,"version":"3.50.1"},"reference-count":24,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T00:00:00Z","timestamp":1771891200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Guangxi Zhuang Autonomous Region Administration of traditional Chinese Medicine","award":["GXZYA20220010"],"award-info":[{"award-number":["GXZYA20220010"]}]},{"name":"2024 Guangxi Food Safety Standards Follow-up Evaluation Program"},{"DOI":"10.13039\/501100017691","name":"Guangxi Key Research and Development Program","doi-asserted-by":"publisher","award":["GUIKE AB24010004"],"award-info":[{"award-number":["GUIKE AB24010004"]}],"id":[{"id":"10.13039\/501100017691","id-type":"DOI","asserted-by":"publisher"}]},{"name":"The Open Project of Key Laboratory of Chinese Medicine Clinical Research, Education Department of Guangxi Zhuang Autonomous Region","award":["GXED-CMCR-05"],"award-info":[{"award-number":["GXED-CMCR-05"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>This study aimed to systematically identify the active constituents of Kadsura coccinea (Lem.) A. C. Smith (KC) and elucidate their potential mechanisms in treating rheumatoid arthritis (RA) using an integrated analytical and computational approach. Chemical profiling of KC root extract was performed by UPLC-Q-TOF-MS\/MS. Active compounds and their targets were predicted using the SwissTargetPrediction database, while RA-related genes were retrieved from OMIM, GeneCards, and DisGeNET. A compound\u2013target network was constructed and analyzed via Cytoscape. Functional enrichment analyses and protein\u2013protein interaction (PPI) clustering were conducted to identify key pathways. Molecular docking was employed to validate interactions between core compounds and key RA targets. A total of 90 compounds were identified, primarily 36 lignans and 29 triterpenoids. Network analysis revealed 145 overlapping targets between KC and RA. These targets were further associated with 65 compounds derived from KC. Key compounds such as kadcoccinone F, kadsuralignan I and schisantherin M were linked to hub targets including MAPK14, MMPs, and JAKs, which are involved in inflammatory signaling, matrix degradation, and immune regulation. Molecular docking confirmed strong binding affinities (\u0394G &lt; \u22125.0 kcal\/mol) between representative KC compounds and targets like MMP1, MMP2, JAK2 and JAK3, supported by analyses of hydrogen bonding, hydrophobic, and \u03c0-interactions. These results suggest that KC exerts anti-RA effects through multi-component, multi-target mechanisms, primarily modulating inflammatory signaling, immune cell recruitment, and tissue-destructive pathways. This study provides a pharmacological basis for the traditional use of KC in RA management and supports its potential as a complementary therapeutic agent.<\/jats:p>","DOI":"10.3390\/ijms27052097","type":"journal-article","created":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T12:19:23Z","timestamp":1771935563000},"page":"2097","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["UPLC-Q-TOF-MS\/MS and Network Pharmacology Approaches to Explore the Active Compounds and Mechanisms of Kadsura coccinea for Treating Rheumatoid Arthritis"],"prefix":"10.3390","volume":"27","author":[{"given":"Liya","family":"Qiao","sequence":"first","affiliation":[{"name":"Guangxi Zhuang Autonomous Region Chinese Medicinal Materials Product Quality Supervision and Inspection Station, Guangxi Botanical Garden of Medicinal Plants, Nanning 530023, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiashui","family":"Liao","sequence":"additional","affiliation":[{"name":"Guangxi Zhuang Autonomous Region Chinese Medicinal Materials Product Quality Supervision and Inspection Station, Guangxi Botanical Garden of Medicinal Plants, Nanning 530023, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongchun","family":"Huang","sequence":"additional","affiliation":[{"name":"Guangxi Zhuang Autonomous Region Chinese Medicinal Materials Product Quality Supervision and Inspection Station, Guangxi Botanical Garden of Medicinal Plants, Nanning 530023, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ping","family":"Li","sequence":"additional","affiliation":[{"name":"Guangxi Zhuang Autonomous Region Chinese Medicinal Materials Product Quality Supervision and Inspection Station, Guangxi Botanical Garden of Medicinal Plants, Nanning 530023, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hairong","family":"Long","sequence":"additional","affiliation":[{"name":"School of Biological Science and Food Engineering, Chuzhou University, Chuzhou 239001, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-4061-244X","authenticated-orcid":false,"given":"Lu","family":"Chen","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of High-Quality Formation and Utilization of Dao-di Herbs, National Engineering Research Center of Southwest Endangered Medicinal Resource Development, Guangxi Botanical Garden of Medicinal Plants, Nanning 530023, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tingting","family":"Tong","sequence":"additional","affiliation":[{"name":"Guangxi Zhuang Autonomous Region Chinese Medicinal Materials Product Quality Supervision and Inspection Station, Guangxi Botanical Garden of Medicinal Plants, Nanning 530023, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaowen","family":"Ji","sequence":"additional","affiliation":[{"name":"Guangxi Zhuang Autonomous Region Chinese Medicinal Materials Product Quality Supervision and Inspection Station, Guangxi Botanical Garden of Medicinal Plants, Nanning 530023, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mengli","family":"Zhang","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of High-Quality Formation and Utilization of Dao-di Herbs, National Engineering Research Center of Southwest Endangered Medicinal Resource Development, Guangxi Botanical Garden of Medicinal Plants, Nanning 530023, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yude","family":"Peng","sequence":"additional","affiliation":[{"name":"Guangxi Zhuang Autonomous Region Chinese Medicinal Materials Product Quality Supervision and Inspection Station, Guangxi Botanical Garden of Medicinal Plants, Nanning 530023, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5014-9415","authenticated-orcid":false,"given":"Yu","family":"Pan","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of High-Quality Formation and Utilization of Dao-di Herbs, National Engineering Research Center of Southwest Endangered Medicinal Resource Development, Guangxi Botanical Garden of Medicinal Plants, Nanning 530023, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xianghua","family":"Xia","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of High-Quality Formation and Utilization of Dao-di Herbs, National Engineering Research Center of Southwest Endangered Medicinal Resource Development, Guangxi Botanical Garden of Medicinal Plants, Nanning 530023, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,2,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2019","DOI":"10.1016\/S0140-6736(23)01525-8","article-title":"Rheumatoid arthritis","volume":"402","author":"Bathon","year":"2023","journal-title":"Lancet"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1194","DOI":"10.1001\/jama.2022.0786","article-title":"What Is Rheumatoid Arthritis?","volume":"327","author":"Smith","year":"2022","journal-title":"JAMA"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"120387","DOI":"10.1016\/j.jep.2025.120387","article-title":"Signal pathways in the treatment of Rheumatoid Arthritis with traditional Chinese medicine","volume":"353","author":"Wang","year":"2025","journal-title":"J. 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