{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T17:57:46Z","timestamp":1771955866863,"version":"3.50.1"},"reference-count":67,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T00:00:00Z","timestamp":1771891200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Program for the Development of Science and Technology of Jilin Province","award":["YDZJ202201ZYTS457"],"award-info":[{"award-number":["YDZJ202201ZYTS457"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Mechanosensitive Piezo1 channels participate in regulating pain sensitivity, insulin secretion, and vascular tension; however, their expression in the autonomic paraventricular nucleus (PVN) and role in modulating sympathetic outflow and cardiovascular function remain unstudied. In this study, unilateral PVN microinjection of the Piezo1 channel blocker Dooku1 (0.1, 1, 10, 100, and 200 pmol) administered to anesthetized male rats increased renal sympathetic nerve activity (RSNA) and mean artery pressure (MAP) in a dose-dependent manner, with maximum increases of 93 \u00b1 30% (p &lt; 0.0001) and 21 \u00b1 5 mmHg (p &lt; 0.0001), respectively, elicited by Dooku1 at 100 pmol. Similarly, PVN microinjection of the peptide Piezo1 channel blocker GsMTx4 (1 nmol) significantly increased RSNA (p &lt; 0.001) and MAP (p &lt; 0.0001). Conversely, PVN-microinjected Piezo1 channel activators Yoda1 (5 nmol) and Jedi2 (5 nmol) did not significantly alter RSNA or MAP. Western blot and qRT-PCR analyses of the hypothalamic PVN showed abundant Piezo1 mRNA and protein expression. Immunofluorescence detection showed that Piezo1 was expressed in pre-sympathetic PVN neurons with axons projecting to the rostral ventrolateral medulla. We conclude that Piezo1 channels expressed in the autonomic PVN neurons play an important role in regulating sympathetic outflow and cardiovascular function.<\/jats:p>","DOI":"10.3390\/ijms27052115","type":"journal-article","created":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T16:58:18Z","timestamp":1771952298000},"page":"2115","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Role of Piezo1 Channels Expressed in PVN in Regulating Sympathetic Nerve Activity and Arterial Blood Pressure in Rats"],"prefix":"10.3390","volume":"27","author":[{"given":"Yue","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Biotechnology, School of Life Science, Jilin Normal University, Siping 136000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Guo","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Dezhou University, Dezhou 253023, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jichun","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, School of Life Science, Jilin Normal University, Siping 136000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, School of Life Science, Jilin Normal University, Siping 136000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuying","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, School of Life Science, Jilin Normal University, Siping 136000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-8816-1410","authenticated-orcid":false,"given":"Gregory J.","family":"Miodonski","sequence":"additional","affiliation":[{"name":"Department of Kinesiology and Integrative Physiology, Michigan Technological University, Houghton, MI 49931-1200, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1344-5431","authenticated-orcid":false,"given":"Enshe","family":"Jiang","sequence":"additional","affiliation":[{"name":"School of Nursing and Health, Institutes of Nursing and Health, Henan University, Kaifeng 450046, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1639-5292","authenticated-orcid":false,"given":"Qing-Hui","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Kinesiology and Integrative Physiology, Michigan Technological University, Houghton, MI 49931-1200, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2685-2722","authenticated-orcid":false,"given":"Yi","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, School of Life Science, Jilin Normal University, Siping 136000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0058-6219","authenticated-orcid":false,"given":"Renjun","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, School of Life Science, Jilin Normal University, Siping 136000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,2,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3458","DOI":"10.1210\/en.2018-00453","article-title":"The Paraventricular Nucleus of the Hypothalamus: Development, Function, and Human Diseases","volume":"159","author":"Qin","year":"2018","journal-title":"Endocrinology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"538","DOI":"10.1016\/j.tins.2021.03.001","article-title":"The paraventricular nucleus of the thalamus: An integrative node underlying homeostatic behavior","volume":"44","author":"Penzo","year":"2021","journal-title":"Trends Neurosci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"e2032232024","DOI":"10.1523\/JNEUROSCI.2032-23.2024","article-title":"GLP-1 in the Hypothalamic Paraventricular Nucleus Promotes Sympathetic Activation and Hypertension","volume":"44","author":"Xu","year":"2024","journal-title":"J. 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