{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T17:26:31Z","timestamp":1789665991541,"version":"4.0.1"},"reference-count":36,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T00:00:00Z","timestamp":1772236800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Fundamental Research Funds for the Central Public Welfare Research Institutes","award":["ZZ14-YQ-052, ZZ15-YQ-062, ZZ15-YQ-063, ZZ15-YQ-064, ZZ17-ND-10-01, ZZ17-ND-10-10, ZZ18-ND-10-08, ZZ19-YQ-051, ZZ19-ND-10-02, ZZ19-ND-10-04, ZZ19-ND-10-09"],"award-info":[{"award-number":["ZZ14-YQ-052, ZZ15-YQ-062, ZZ15-YQ-063, ZZ15-YQ-064, ZZ17-ND-10-01, ZZ17-ND-10-10, ZZ18-ND-10-08, ZZ19-YQ-051, ZZ19-ND-10-02, ZZ19-ND-10-04, ZZ19-ND-10-09"]}]},{"name":"Hebei Innovation Capability Enhancement Program","award":["24462502D"],"award-info":[{"award-number":["24462502D"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Chicory (Cichorium intybus L.) is a widely used nutritional and medicinal plant, whose roots are an important commercial source of inulin, while the aerial parts are often discarded during industrial processing. This study systematically compared chicory polysaccharides (CPs) extracted from aerial parts (CP-A) and roots (CP-R) with respect to their compositional features and cytoprotective effects in an oxygen\u2013glucose deprivation\/reperfusion (OGD\/R)-induced H9c2 cell injury model. CP-A and CP-R differed in molecular weight distribution and monosaccharide composition, with CP-R exhibiting a higher molecular weight and fructose content. Despite these differences, both fractions significantly improved cell viability and reduced oxidative and biochemical injury markers. Integrated proteomic and transcriptomic analyses indicated that CP-A and CP-R were associated with the modulation of stress-responsive signaling networks, prominently involving oxidative stress-linked MAPK\/NF-\u03baB pathways. These findings demonstrate comparable cytoprotective activities of polysaccharide-rich fractions from roots and aerial parts and support the valorization of chicory aerial biomass as a potential source of functional ingredients for cardiovascular health.<\/jats:p>","DOI":"10.3390\/ijms27052303","type":"journal-article","created":{"date-parts":[[2026,3,2]],"date-time":"2026-03-02T12:39:56Z","timestamp":1772455196000},"page":"2303","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Comparative Analysis of Polysaccharides from Chicory Roots and Aerial Parts Reveals Comparable Cytoprotective Effects Associated with MAPK\/NF-\u03baB Signaling"],"prefix":"10.3390","volume":"27","author":[{"given":"Yi","family":"Ying","sequence":"first","affiliation":[{"name":"State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, Artemisinin Research Center, and Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ang","family":"Ma","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, Artemisinin Research Center, and Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shujie","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, Artemisinin Research Center, and Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-9614-372X","authenticated-orcid":false,"given":"Wenfeng","family":"Qiu","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Henan University, Kaifeng 475004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongda","family":"Xuan","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, Artemisinin Research Center, and Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingchun","family":"Wang","sequence":"additional","affiliation":[{"name":"Inner Mongolia Academy of Traditional Chinese and Mongolian Medicine, Hohhot 010020, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiaoli","family":"Shi","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, Artemisinin Research Center, and Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Chai","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, Artemisinin Research Center, and Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-5057-6358","authenticated-orcid":false,"given":"Dandan","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, Artemisinin Research Center, and Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3014-6364","authenticated-orcid":false,"given":"Hai-Ning","family":"Lyu","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, Artemisinin Research Center, and Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,2,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"127676","DOI":"10.1016\/j.foodchem.2020.127676","article-title":"Chicory (Cichorium intybus L.) as a Food Ingredient\u2014Nutritional Composition, Bioactivity, Safety, and Health Claims: A Review","volume":"336","author":"Krulj","year":"2021","journal-title":"Food Chem."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Janda, K., Gutowska, I., Geszke-Moritz, M., and Jakubczyk, K. (2021). The Common Chicory (Cichorium intybus L.) as a Source of Extracts with Health-Promoting Properties\u2014A Review. Molecules, 26.","DOI":"10.3390\/molecules26061814"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Birsa, M.L., and Sarbu, L.G. (2023). Health Benefits of Key Constituents in Cichorium intybus L.. Nutrients, 15.","DOI":"10.3390\/nu15061322"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Zhao, Y., Zhang, Y., Luo, Y., Chen, F., Qiang, M., Liu, M., Pan, R., Chang, Q., Wang, N., and Usmani, M.W. (2025). Anxiolytic Effects of Cichorium intybus L. Oligo-Polysaccharides by Modulating Gut Microbiota, Neuronal Signaling Pathways, and Neuroinflammation in Chronic Sleep Deprivation-Stressed Mice. Foods, 14.","DOI":"10.3390\/foods14111859"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"103086","DOI":"10.1016\/j.fochx.2025.103086","article-title":"Optimization of Compound Enzyme\u2013Ultrasound Extraction of Chicory Polysaccharides and Their Effects on the Quality of Fermented Milk","volume":"31","author":"Ning","year":"2025","journal-title":"Food Chem. X"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Duda, \u0141., Budryn, G., Olszewska, M.A., Rutkowska, M., Kruczkowska, W., Grabowska, K., Ko\u0142at, D., Ja\u015bkiewicz, A., Pasieka, Z.W., and K\u0142osi\u0144ski, K.K. (2025). Evaluation of Inulin and Polyphenol Content and the Cytotoxicity of Cichorium Intybus L. Var. Foliosum Root Extracts Obtained by Pectinase- and Pressure-Assisted Extraction. Nutrients, 17.","DOI":"10.3390\/nu17061040"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"5659","DOI":"10.1007\/s11101-025-10085-x","article-title":"The Chicory Root (Cichorium intybus var. sativum) Frontier: Pioneering Biotechnological Advancements","volume":"24","author":"Akram","year":"2025","journal-title":"Phytochem. Rev."},{"key":"ref_8","first-page":"102201","article-title":"Photoprotective and Anti-Inflammatory Potential of Polyphenol-Rich Extracts from Agricultural by-Products of Chicory Leaves, Red Onion Peel, Vineyard Pruning, and Olive Pruning in UV-B-Exposed 3D Human Skin Equivalents","volume":"23","author":"Tchoumtchoua","year":"2025","journal-title":"J. Agric. Food Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"e70550","DOI":"10.1002\/fsn3.70550","article-title":"Chicory (Cichorium intybus) Leaves Extract: Phenolic Composition, Antibacterial Activity, and Antioxidant Capacity Assessment","volume":"13","author":"Mohammed","year":"2025","journal-title":"Food Sci. Nutr."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Krepkova, L.V., Babenko, A.N., Lemyaseva, S.V., Saybel, O.L., Sherwin, C.M., and Enioutina, E.Y. (2023). Modulation of Hepatic Functions by Chicory (Cichorium intybus L.) Extract: Preclinical Study in Rats. Pharmaceuticals, 16.","DOI":"10.3390\/ph16101471"},{"key":"ref_11","first-page":"40","article-title":"Composition of Chicory Root, Peel, Seed and Leaf Ethanol Extracts and Biological Properties of Their Non-Inulin Fractions","volume":"49","author":"Jurgobnski","year":"2011","journal-title":"Food Technol. Biotechnol."},{"key":"ref_12","first-page":"1060","article-title":"Determination of Some Biochemicals, Phytochemicals and Antioxidant Properties of Different Parts of Cichorium intybus L.: A Comparative Study","volume":"23","author":"Shad","year":"2013","journal-title":"J. Anim. Plant Sci."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Leyane, T.S., Jere, S.W., and Houreld, N.N. (2022). Oxidative Stress in Ageing and Chronic Degenerative Pathologies: Molecular Mechanisms Involved in Counteracting Oxidative Stress and Chronic Inflammation. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23137273"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Zhang, Z., and Guo, J. (2025). Deciphering Oxidative Stress in Cardiovascular Disease Progression: A Blueprint for Mechanistic Understanding and Therapeutic Innovation. Antioxidants, 14.","DOI":"10.3390\/antiox14010038"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"31348","DOI":"10.1021\/acs.jafc.5c09909","article-title":"Rosmarinic Acid Suppresses Keap1\/Nrf2 Signaling Pathway via Targeting USP15 to Attenuate Myocardial Ischemia\/Reperfusion Injury","volume":"73","author":"Zhao","year":"2025","journal-title":"J. Agric. Food Chem."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"141385","DOI":"10.1016\/j.ijbiomac.2025.141385","article-title":"Extraction, Optimization, Purification and Characterization of Inulin from Chicory Roots Using Conventional and Greener Extraction Techniques","volume":"306","author":"Singh","year":"2025","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1016\/j.foodhyd.2017.10.004","article-title":"Sequential Extraction and Physicochemical Characterization of Polysaccharides from Chicory (Cichorium intybus) Root Pulp","volume":"77","author":"Guo","year":"2018","journal-title":"Food Hydrocoll."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"6077","DOI":"10.1021\/jf401621h","article-title":"Structural and Water-Holding Characteristics of Untreated and Ensiled Chicory Root Pulp","volume":"61","author":"Ramasamy","year":"2013","journal-title":"J. Agric. Food Chem."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Zou, X., Cai, J., Xiao, J., Zhang, M., Jia, X., Dong, L., Hu, K., Yi, Y., Zhang, R., and Huang, F. (2023). Purification, Characterization and Bioactivity of Different Molecular-Weight Fractions of Polysaccharide Extracted from Litchi Pulp. Foods, 12.","DOI":"10.3390\/foods12010194"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Zeng, W., Chen, L., Xiao, Z., Li, Y., Ma, J., Ding, J., and Yang, J. (2023). Comparative Study on the Structural Properties and Bioactivities of Three Different Molecular Weights of Lycium barbarum Polysaccharides. Molecules, 28.","DOI":"10.3390\/molecules28020701"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Xuan, H.-D., Shen, S.-N., Fu, C.-J., Zhou, J., Chen, R.-X., Zhang, J.-Z., Liu, X.-X., Li, F.-Q., Zhao, Y.-F., and Zhang, D. (2025). Enhancing the Anti-Inflammatory Efficacy of Polysaccharides through Processing Techniques: A Case Study on Atractylodes macrocephala. Nat. Prod. Res., 1\u20139.","DOI":"10.1080\/14786419.2025.2548615"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"116357","DOI":"10.1016\/j.carbpol.2020.116357","article-title":"Chemical structure elucidation of an inulin-type fructan isolated from Lobelia chinensis lour with anti-obesity activity on diet-induced mice","volume":"240","author":"Zhang","year":"2020","journal-title":"Carbohydr. Polym."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Wang, X.-F., Chen, X., Tang, Y., Wu, J.-M., Qin, D.-L., Yu, L., Yu, C.-L., Zhou, X.-G., and Wu, A.-G. (2022). The Therapeutic Potential of Plant Polysaccharides in Metabolic Diseases. Pharmaceuticals, 15.","DOI":"10.3390\/ph15111329"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Liu, W., Jiang, J., Tang, Z., Yang, Z., and Qin, J. (2025). De Novo Transcriptome Sequencing Analysis Revealed the Expression Patterns of Genes in Different Organs and the Molecular Basis of Polysaccharide Synthesis in Bletilla striata. Genes, 16.","DOI":"10.3390\/genes16050558"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1016\/j.carbpol.2013.09.081","article-title":"Inulin-type fructans: A review on different aspects of biochemical and pharmaceutical technology","volume":"101","author":"Pessoa","year":"2014","journal-title":"Carbohydr. Polym."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"746","DOI":"10.1002\/jsfa.12967","article-title":"Bioactive Polysaccharides in Different Plant Parts of Aconitum carmichaelii","volume":"104","author":"Fu","year":"2024","journal-title":"J. Sci. Food Agric."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Feng, S., Tang, M., Jiang, Z., Ruan, Y., Liu, L., Kong, Q., Xiang, Z., Chen, T., Zhou, L., and Yang, H. (2022). Optimization of Extraction Process, Structure Characterization, and Antioxidant Activity of Polysaccharides from Different Parts of Camellia oleifera Abel. Foods, 11.","DOI":"10.3390\/foods11203185"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Qiu, X., Ou, Y., Lu, S., Liang, Y., Zhang, Y., Li, M., Li, G., Ma, H., Wu, Y., and He, Z. (2024). Study of the Structure and Bioactivity of Polysaccharides from Different Parts of Stemona tuberosa Lour. Molecules, 29.","DOI":"10.3390\/molecules29061347"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1080\/09540105.2020.1742667","article-title":"Green Chicory Leaf Extract Exerts Anti-Inflammatory Effects through Suppressing LPS-Induced MAPK\/NF-\u03baB Activation and Hepatoprotective Activity in Vitro","volume":"31","author":"Bayazid","year":"2020","journal-title":"Food Agric. Immunol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ijbiomac.2017.05.169","article-title":"A Polysaccharide of Dendrobium officinale Ameliorates H2O2-Induced Apoptosis in H9c2 Cardiomyocytes via PI3K\/AKT and MAPK Pathways","volume":"104","author":"Zhang","year":"2017","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"138207","DOI":"10.1016\/j.ijbiomac.2024.138207","article-title":"A Polysaccharide from Glycyrrhiza uralensis Attenuates Myocardial Fibrosis via Modulating the MAPK\/PI3K\/AKT Signaling Pathway","volume":"286","author":"Niu","year":"2025","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Wang, X., Liu, R., and Liu, D. (2025). The Role of the MAPK Signaling Pathway in Cardiovascular Disease: Pathophysiological Mechanisms and Clinical Therapy. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms26062667"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"e40812","DOI":"10.1016\/j.heliyon.2024.e40812","article-title":"Investigating the NF-\u03baB Signaling Pathway in Heart Failure: Exploring Potential Therapeutic Approaches","volume":"10","author":"Ghiasi","year":"2024","journal-title":"Heliyon"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"18891","DOI":"10.1038\/s41598-023-46033-y","article-title":"Daeshiho-Tang Attenuates Inflammatory Response and Oxidative Stress in LPS-Stimulated Macrophages by Regulating TLR4\/MyD88, NF-\u03baB, MAPK, and Nrf2\/HO-1 Pathways","volume":"13","author":"Oh","year":"2023","journal-title":"Sci. Rep."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"e14603","DOI":"10.1111\/cns.14603","article-title":"Transcription Factor CEBPB Mediates Intracranial Aneurysm Rupture by Inflammatory and Immune Response","volume":"30","author":"Tian","year":"2024","journal-title":"CNS Neurosci. Ther."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1831","DOI":"10.1038\/s41467-026-68542-w","article-title":"1,3-and 1,4-linked polysaccharides uptake in intestinal cells relies on clathrin\/dynamin 1\/Rab5-dependent endocytosis","volume":"17","author":"Liao","year":"2026","journal-title":"Nat. Commun."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/5\/2303\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T05:19:54Z","timestamp":1772687994000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/5\/2303"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,28]]},"references-count":36,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2026,3]]}},"alternative-id":["ijms27052303"],"URL":"https:\/\/doi.org\/10.3390\/ijms27052303","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,28]]}}}