{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T23:49:54Z","timestamp":1773272994171,"version":"3.50.1"},"reference-count":32,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T00:00:00Z","timestamp":1773187200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Chemotherapy has significantly improved survival in breast cancer and, in the neoadjuvant setting, contributes to tumor downstaging and increased rates of breast-conserving surgery while enabling in vivo assessment of tumor biology and chemosensitivity. Pathological complete response (pCR) is a key endpoint associated with favorable outcomes; however, tumor heterogeneity highlights the need for reliable predictive biomarkers. This study evaluated the mRNA expression of 13 candidate genes in relation to molecular subtypes and pathological response to neoadjuvant chemotherapy (NAC) to identify potential predictive and prognostic markers. Pretreatment core biopsies from 92 patients receiving NAC were analyzed by quantitative RT-PCR. Molecular subtypes were determined by immunohistochemistry (ER, PR, HER2, Ki67), and pathological response was classified using the Miller\u2013Payne scale as good (MP 4\/5) or poor (MP 1\u20133). Multivariate logistic regression assessed associations between gene expression, subtype, and pCR. Hormone receptor-positive tumors showed significantly higher expression of AXL, FGFR1, RAP80, GAS6, BTRCP, and ZNF217. Significant associations with pCR were observed for AXL, FGFR1, YAP, and BRCA1. Low AXL and BRCA1 expression levels were independently associated with pCR. In addition, their combined low expression was associated most strongly with breast pCR in this cohort. These findings should be interpreted as exploratory and require validation in independent cohorts.<\/jats:p>","DOI":"10.3390\/ijms27062564","type":"journal-article","created":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T09:00:37Z","timestamp":1773219637000},"page":"2564","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Gene Expression Profiles Associated with Molecular Subtypes and Pathological Response to Neoadjuvant Treatment in Surgical Breast Cancer"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7670-5603","authenticated-orcid":false,"given":"Sonia","family":"Baulies","sequence":"first","affiliation":[{"name":"Gynecologic Oncology and Breast Pathology Unit, Department of Obstetrics, Gynecology, and Reproduction, Dexeus Mujer, Dexeus University Hospital, 08028 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8866-9881","authenticated-orcid":false,"given":"Miguel Angel","family":"Molina-Vila","sequence":"additional","affiliation":[{"name":"Pangaea Oncology, Laboratory of Molecular Biology, Coyote Research Group, Dexeus University Hospital, 08028 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francesc","family":"Tresserra","sequence":"additional","affiliation":[{"name":"Pathology Unit, Department of Obstetrics, Gynecology, and Reproduction, Dexeus Mujer, Dexeus University Hospital, 08028 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6586-0111","authenticated-orcid":false,"given":"Ignacio","family":"Rodr\u00edguez","sequence":"additional","affiliation":[{"name":"Statistics and Epidemiology Unit, Department of Obstetrics, Gynecology, and Reproduction, Dexeus Mujer, Dexeus University Hospital, 08028 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yannick","family":"Hurni","sequence":"additional","affiliation":[{"name":"Gynecologic Oncology and Breast Pathology Unit, Department of Obstetrics, Gynecology, and Reproduction, Dexeus Mujer, Dexeus University Hospital, 08028 Barcelona, Spain"},{"name":"Department of Paediatrics, Obstetrics, Gynaecology and Preventive Medicine and Public Health, Faculty of Medicine, Universidad Autonoma Barcelona, 08193 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana","family":"Gim\u00e9nez-Capit\u00e1n","sequence":"additional","affiliation":[{"name":"Pangaea Oncology, Laboratory of Molecular Biology, Coyote Research Group, Dexeus University Hospital, 08028 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3164-6686","authenticated-orcid":false,"given":"Silvia","family":"Cabrera","sequence":"additional","affiliation":[{"name":"Gynecologic Oncology and Breast Pathology Unit, Department of Obstetrics, Gynecology, and Reproduction, Dexeus Mujer, Dexeus University Hospital, 08028 Barcelona, Spain"},{"name":"Department of Paediatrics, Obstetrics, Gynaecology and Preventive Medicine and Public Health, Faculty of Medicine, Universidad Autonoma Barcelona, 08193 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rafael","family":"F\u00e1bregas","sequence":"additional","affiliation":[{"name":"Gynecologic Oncology and Breast Pathology Unit, Department of Obstetrics, Gynecology, and Reproduction, Dexeus Mujer, Dexeus University Hospital, 08028 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,3,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/S1470-2045(17)30777-5","article-title":"Long-term outcomes for neoadjuvant versus adjuvant chemotherapy in early breast cancer: Meta-analysis of individual patient data from ten randomised trials","volume":"19","author":"Asselain","year":"2018","journal-title":"Lancet Oncol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1093\/oxfordjournals.jncimonographs.a003469","article-title":"Preoperative chemotherapy in patients with operable breast cancer: Nine-year results from national surgical adjuvant breast and bowel project b-18","volume":"2001","author":"Wolmark","year":"2001","journal-title":"J. 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