{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T09:08:46Z","timestamp":1773306526087,"version":"3.50.1"},"reference-count":62,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T00:00:00Z","timestamp":1773187200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003787","name":"Natural Science Foundation of Hebei Province","doi-asserted-by":"publisher","award":["H2025206764"],"award-info":[{"award-number":["H2025206764"]}],"id":[{"id":"10.13039\/501100003787","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Science and Technology Program Project of Hebei","award":["252W2501D"],"award-info":[{"award-number":["252W2501D"]}]},{"name":"University Student Innovation Training Program","award":["USIP20241047"],"award-info":[{"award-number":["USIP20241047"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Paeoniae Radix Alba Carbonisata (PRAC), carbonized decoction pieces of the traditional Chinese medicine Paeoniae Radix Alba, has been used in clinical practice for hepatoprotective purposes. Hepatic amyloidosis (HA), a severe complication of systemic AA amyloidosis, is characterized by the deposition of fibrillar amyloid proteins leading to progressive hepatic dysfunction. However, its role in HA remains unclear. Amyloid lysozyme (LYSO-6) was used to induce the NCTC1469 cell injury model and the HA mouse model. The effects of PRAC extract (PRAC-E) on liver injury were then evaluated using biochemical assays, enzyme-linked immunosorbent assay (ELISA), Congo red (CR) staining, Hematoxylin and Eosin (H&amp;E) staining, and immunohistochemical staining. Liver transcriptomics combined with Western blotting was used to analyze the expression levels of key proteins in the cGMP\/PKG\/ATP2A1 signaling axis. UHPLC-Q-Exactive Orbitrap MS combined with network pharmacology was used to characterize the chemical components of PRAC-E and identify its core active constituents against HA. Quantitative analysis of core components was performed by UHPLC-QTRAP-MS\/MS. Molecular docking predicted the binding stability of core components and key targets. The results showed that PRAC-E significantly alleviated HA. Collectively, PRAC-E restored calcium pump activity, corrected calcium homeostasis imbalance, reduced inflammatory factor levels, regulated Phosphodiesterase 5A (PDE5A), and activated the cGMP\/PKG\/ATP2A1 signaling axis. The main components of PRAC-E were phenolic acids, terpenoids, and flavonoids. Among these, six core components (SCCs) related to HA were Gallate (16.96 mg\/g), Paeoniflorin (14.27 mg\/g), Albiflorin (7.20 mg\/g), Benzoyl paeoniflorin (5.33 mg\/g), Methyl gallate (0.78 mg\/g), and Catechin (0.09 mg\/g). Molecular docking analysis demonstrated that SCCs formed stable complexes (\u2206G \u2264 \u22126.2 kcal\/mol) with ATP2A1.<\/jats:p>","DOI":"10.3390\/ijms27062582","type":"journal-article","created":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T14:43:18Z","timestamp":1773240198000},"page":"2582","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Protective Effect of Paeoniae Radix Alba Carbonisata on Hepatic Amyloidosis by Regulating Calcium Homeostasis"],"prefix":"10.3390","volume":"27","author":[{"given":"Gangqiang","family":"Liu","sequence":"first","affiliation":[{"name":"School of Pharmacy, Hebei Medical University, Shijiazhuang 050017, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zerui","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Hebei Medical University, Shijiazhuang 050017, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huihui","family":"Xu","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Hebei Medical University, Shijiazhuang 050017, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinyu","family":"Jia","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Hebei Medical University, Shijiazhuang 050017, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhong","family":"Xue","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Hebei Medical University, Shijiazhuang 050017, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Ge","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Hebei Medical University, Shijiazhuang 050017, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xueqing","family":"Ji","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Hebei Medical University, Shijiazhuang 050017, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lijian","family":"Cui","sequence":"additional","affiliation":[{"name":"Experiment Center, Hebei University of Chinese Medicine, Shijiazhuang 050020, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yun","family":"Huang","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Hebei Medical University, Shijiazhuang 050017, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,3,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1159\/000506617","article-title":"When to suspect a diagnosis of amyloidosis","volume":"143","author":"Vaxman","year":"2020","journal-title":"Acta Haematol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1007\/s12016-019-08759-4","article-title":"Systemic amyloidosis: A contemporary overview","volume":"59","author":"Aguanno","year":"2020","journal-title":"Clin. 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