{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T11:05:57Z","timestamp":1773659157781,"version":"3.50.1"},"reference-count":62,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T00:00:00Z","timestamp":1773446400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"Korean government","doi-asserted-by":"publisher","award":["2022M3A9E4095340"],"award-info":[{"award-number":["2022M3A9E4095340"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003664","name":"Korea Forest Service","doi-asserted-by":"publisher","award":["RS-2024-00404789"],"award-info":[{"award-number":["RS-2024-00404789"]}],"id":[{"id":"10.13039\/501100003664","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Inflammatory bowel disease (IBD) is triggered by genetic predisposition and chronic inflammation, with aberrant activation of the innate immune complex NLRP3 inflammasome playing a pivotal role in its pathogenesis. In this study, we investigated the effects of a hot water extract from the brown alga Endarachne binghamiae (EB-WE) on the inhibition of NLRP3 inflammasome activation, with a focus on its antioxidant properties, in various inflammation models. In bone marrow-derived macrophages (BMDMs), NLRP3 inflammasome activation was induced using LPS and ATP, and EB-WE pretreatment (100, 200 \u00b5g\/mL) significantly reduced the secretion of IL-1\u03b2 and IL-18. Confocal immunofluorescence analysis further confirmed that EB-WE suppressed the formation of the NLRP3-ASC\/caspase-1 complex. Furthermore, the in vivo anti-IBD efficacy of EB-WE was assessed using a DSS-induced mouse model, in which colonic inflammation and NLRP3-mediated responses were prominent. Oral administration of EB-WE (2 or 5 mg\/day) markedly ameliorated clinical symptoms, such as weight loss, diarrhea, and rectal bleeding, and significantly reduced the disease activity index (DAI). EB-WE also decreased serum pro-inflammatory cytokine levels and the expression of NLRP3 inflammasome-related molecules in colon tissue at both the gene and protein levels. In both BMDMs and the IBD mouse model, we further analyzed the upstream regulatory pathway involving NOX2-iNOS. EB-WE efficiently inhibited the activation of the NOX-iNOS axis and NF-\u03baB phosphorylation, thereby alleviating inflammasome activation associated with DSS-induced oxidative stress and neutrophil\/macrophage infiltration. Collectively, these results demonstrate that EB-WE effectively suppresses the formation and activation of the NLRP3 inflammasome by modulating the NOX-iNOS axis and the NF-\u03baB pathway via antioxidant mechanisms. These findings suggest that EB-WE holds promise as a novel marine-derived natural therapeutic agent for the treatment of chronic inflammatory diseases.<\/jats:p>","DOI":"10.3390\/ijms27062674","type":"journal-article","created":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T10:10:43Z","timestamp":1773655843000},"page":"2674","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Endarachne binghamiae Extract Alleviates Colitis by Suppressing NLRP3 Inflammasome Activation via Regulation of NOX\u2013iNOS Crosstalk"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-2767-2065","authenticated-orcid":false,"given":"Sang Seop","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Microbiology, College of Medicine, Konyang University, Daejon 32992, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sang Hoon","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Microbiology, College of Medicine, Konyang University, Daejon 32992, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"So Yeon","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Microbiology, College of Medicine, Konyang University, Daejon 32992, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bong Ho","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biological Engineering, College of Engineering, Hanbat National University, Daejon 34158, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8905-6104","authenticated-orcid":false,"given":"Yung-Choon","family":"Yoo","sequence":"additional","affiliation":[{"name":"Department of Microbiology, College of Medicine, Konyang University, Daejon 32992, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,3,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"116679","DOI":"10.1016\/j.biopha.2024.116679","article-title":"NLRP3 inflammasome and its role in autoimmune diseases: A promising therapeutic target","volume":"175","author":"Ren","year":"2024","journal-title":"Biomed. 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