{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T06:59:24Z","timestamp":1783407564175,"version":"3.54.6"},"reference-count":49,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T00:00:00Z","timestamp":1773878400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Science and Technology Department of Sichuan Province","award":["2024YFHZ0334"],"award-info":[{"award-number":["2024YFHZ0334"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The gut\u2013brain axis is increasingly implicated in Alzheimer\u2019s disease (AD) pathogenesis, but the potential correlation between AD-associated gut microbiota and central inflammation remains largely unclear. This study aimed to explore their correlative link, with a focus on changes and involvement of Th17 cell-related factors in the gut\u2013brain axis. Healthy C57BL\/6J mice were pretreated with antibiotics for 1 week to deplete the indigenous gut microbiota, followed by 2 weeks of fecal microbiota transplantation (FMT) using feces from APP\/PS1 AD model mice. Hematoxylin\u2013eosin (H&amp;E) staining, ELISA, reverse transcription-quantitative polymerase chain reaction (RT-qPCR), 16S rDNA sequencing, and correlation analysis were performed to evaluate ileal and central pathological changes, Th17 cell-related inflammatory mediators, ileal microbiota composition, and their potential correlations. The results demonstrated that AD-FMT significantly induced ileal inflammatory infiltration and central inflammation in recipient mice, which was accompanied by abnormal expression of Th17 cell-related indicators, elevated levels of Th17-associated inflammatory factors, upregulated ROR\u03b3t mRNA expression, and perturbed ileal microbiota composition. Correlation analysis further suggested that specific ileal bacterial taxa were closely correlated with Th17 cell-related inflammatory factors. These findings suggest a potential correlation between AD-associated microbiota and central inflammation, possibly by regulating intestinal Th17 cell-related indicators and altering gut microbial composition. This study provides correlative evidence supporting the involvement of the gut\u2013brain axis in AD-related pathogenesis, highlighting the link between gut microbiota, central inflammation and Th17-related factors.<\/jats:p>","DOI":"10.3390\/ijms27062791","type":"journal-article","created":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T11:50:36Z","timestamp":1773921036000},"page":"2791","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Fecal Microbiota Transplantation from APP\/PS1 Mice Induces Th17-Related Inflammatory Parameters and Pathological Changes in the Gut\u2013Brain Axis of Healthy C57BL\/6J Mice"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-9046-5950","authenticated-orcid":false,"given":"Dongni","family":"Lei","sequence":"first","affiliation":[{"name":"School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaomeng","family":"Zhou","sequence":"additional","affiliation":[{"name":"School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-1363-8763","authenticated-orcid":false,"given":"Hao","family":"Zheng","sequence":"additional","affiliation":[{"name":"School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-2701-8237","authenticated-orcid":false,"given":"Yu","family":"Kang","sequence":"additional","affiliation":[{"name":"School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiyong","family":"Yan","sequence":"additional","affiliation":[{"name":"School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,3,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1016\/S0140-6736(15)01124-1","article-title":"Alzheimer\u2019s disease","volume":"388","author":"Scheltens","year":"2016","journal-title":"Lancet"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"736","DOI":"10.1177\/13872877251331236","article-title":"Dilemmas in diagnosing Alzheimer\u2019s disease: The peril and promise of self-fulfilling prophecies","volume":"105","author":"Malek","year":"2025","journal-title":"J. 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