{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T12:20:10Z","timestamp":1774441210113,"version":"3.50.1"},"reference-count":52,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T00:00:00Z","timestamp":1774396800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Science and Higher Education of the Russian Federation\n\t\t\t\t\t\t\t              https:\/\/ror.org\/00ghqgy32","award":["#AAAA-A18-118011990043-5"],"award-info":[{"award-number":["#AAAA-A18-118011990043-5"]}]},{"name":"Ministry of Science and Higher Education of the Russian Federation\n\t\t\t\t\t\t\t              https:\/\/ror.org\/00ghqgy32","award":["#AAAA-A20-120120790036-3"],"award-info":[{"award-number":["#AAAA-A20-120120790036-3"]}]},{"name":"Ministry of Science and Higher Education of the Russian Federation\n\t\t\t\t\t\t\t              https:\/\/ror.org\/00ghqgy32","award":["#123051600030-1"],"award-info":[{"award-number":["#123051600030-1"]}]},{"name":"Ministry of Science and Higher Education of the Russian Federation\n\t\t\t\t\t\t\t              https:\/\/ror.org\/00ghqgy32","award":["#126020216231-0"],"award-info":[{"award-number":["#126020216231-0"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Previously, the role of decreased biodiversity of gut microbiota in polycystic ovary syndrome (PCOS) was demonstrated, but the objective criteria for assessing the representation of microorganisms associated with hyperandrogenemia (HA) were limited. A total of 175 premenopausal women (26 women with PCOS and HA and 149 women without HA, including 19 healthy controls) were recruited during the Eastern Siberia PCOS Epidemiology and Phenotype (ESPEP) Study (2016\u20132019). Methods included a questionnaire survey, clinical examination, pelvic U\/S, blood and feces sampling. Gut microbiome was analyzed by high-throughput sequencing of the V1\u2013V3 of the variable regions of the 16S rRNA gene (Illumina MiSeq, San Diego, CA, USA). Amplicon libraries of 16S rDNA were processed using the QIIME2 bioinformatics pipeline. All data were analyzed using R 3.6.3. The gut microbiocenosis in women with HA was characterized by a higher representation of Lactobacillus and a lower prevalence of the Clostridia class. For Faecalibacterium, Christensenellaceae_R-7_group, and [Eubacterium] eligens group the cut-off values of their relative presence, associated with HA, were estimated as: \u22640.043%, \u22640.039%, and \u22640.02%, respectively. Conclusions: Women with PCOS-associated HA demonstrate a lower prevalence, predominantly, of Clostridia class gut microorganisms, compared with those without any forms of HA. The study presents the quantitative criteria for assessing the representation of gut microorganisms, negatively associated with hyperandrogenic phenotypes of PCOS. The threshold values proposed may be useful to justify the administration of probiotics in PCOS patients with HA.<\/jats:p>","DOI":"10.3390\/ijms27072974","type":"journal-article","created":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T09:11:14Z","timestamp":1774429874000},"page":"2974","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Gut Microorganisms as Markers of Hyperandrogenemia in Premenopausal Women with Polycystic Ovary Syndrome"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6271-7803","authenticated-orcid":false,"given":"Larisa","family":"Suturina","sequence":"first","affiliation":[{"name":"Federal State Budgetary Scientific Institution \u201cScientific Centre for Family Health and Human Reproduction Problems\u201d, 16, Timiryazeva Str., 664003 Irkutsk, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9720-068X","authenticated-orcid":false,"given":"Natalia","family":"Belkova","sequence":"additional","affiliation":[{"name":"Federal State Budgetary Scientific Institution \u201cScientific Centre for Family Health and Human Reproduction Problems\u201d, 16, Timiryazeva Str., 664003 Irkutsk, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tuyana","family":"Sidorova","sequence":"additional","affiliation":[{"name":"Federal State Budgetary Scientific Institution \u201cScientific Centre for Family Health and Human Reproduction Problems\u201d, 16, Timiryazeva Str., 664003 Irkutsk, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-3614-6631","authenticated-orcid":false,"given":"Nadezhda","family":"Smurova","sequence":"additional","affiliation":[{"name":"Federal State Budgetary Scientific Institution \u201cScientific Centre for Family Health and Human Reproduction Problems\u201d, 16, Timiryazeva Str., 664003 Irkutsk, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ilia","family":"Igumnov","sequence":"additional","affiliation":[{"name":"Federal State Budgetary Scientific Institution \u201cScientific Centre for Family Health and Human Reproduction Problems\u201d, 16, Timiryazeva Str., 664003 Irkutsk, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7662-8529","authenticated-orcid":false,"given":"Lyudmila","family":"Lazareva","sequence":"additional","affiliation":[{"name":"Federal State Budgetary Scientific Institution \u201cScientific Centre for Family Health and Human Reproduction Problems\u201d, 16, Timiryazeva Str., 664003 Irkutsk, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irina","family":"Danusevich","sequence":"additional","affiliation":[{"name":"Federal State Budgetary Scientific Institution \u201cScientific Centre for Family Health and Human Reproduction Problems\u201d, 16, Timiryazeva Str., 664003 Irkutsk, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iana","family":"Nadeliaeva","sequence":"additional","affiliation":[{"name":"Federal State Budgetary Scientific Institution \u201cScientific Centre for Family Health and Human Reproduction Problems\u201d, 16, Timiryazeva Str., 664003 Irkutsk, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leonid","family":"Sholokhov","sequence":"additional","affiliation":[{"name":"Federal State Budgetary Scientific Institution \u201cScientific Centre for Family Health and Human Reproduction Problems\u201d, 16, Timiryazeva Str., 664003 Irkutsk, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liliia","family":"Belenkaia","sequence":"additional","affiliation":[{"name":"Federal State Budgetary Scientific Institution \u201cScientific Centre for Family Health and Human Reproduction Problems\u201d, 16, Timiryazeva Str., 664003 Irkutsk, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3407-9365","authenticated-orcid":false,"given":"Alina","family":"Atalyan","sequence":"additional","affiliation":[{"name":"Federal State Budgetary Scientific Institution \u201cScientific Centre for Family Health and Human Reproduction Problems\u201d, 16, Timiryazeva Str., 664003 Irkutsk, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,3,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"211","DOI":"10.23736\/S0026-4784.19.04404-6","article-title":"Criteria, phenotypes and prevalence of polycystic ovary syndrome","volume":"71","author":"Belenkaia","year":"2019","journal-title":"Minerva Ginecol."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Nandakumar, M., Das, P., Sathyapalan, T., Butler, A.E., and Atkin, S.L. 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