{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T15:40:29Z","timestamp":1785426029582,"version":"3.56.0"},"reference-count":39,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T00:00:00Z","timestamp":1774828800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Natural Science Foundation of Nanping City, China","award":["N2025J006"],"award-info":[{"award-number":["N2025J006"]}]},{"name":"Scientific Research Startup Fund for Introduced Talents, Wuyi University","award":["YJ202526"],"award-info":[{"award-number":["YJ202526"]}]},{"award":["YJ202526"],"award-info":[{"award-number":["YJ202526"]}],"id":[{"id":"https:\/\/ror.org\/0488wz367","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>To explore the application potential of insect-derived functional microorganisms in short-cycle leafy vegetable production, we evaluated the effects of Serratia marcescens BRC-CXG2, isolated from larvae of Monochamus alternatus, on romaine lettuce in a pot experiment. Plant growth traits, biomass accumulation, nutritional quality, endogenous hormones, and rhizosphere microbial communities were systematically evaluated. The results demonstrated that inoculation significantly promoted seedling development. Plant height and root length increased by 48.7% and 29.1%, respectively, while shoot and root dry weights were 1.78- and 1.85-fold higher than those of the control. Vitamin C and total sugar contents increased by 76.4% and 98%, respectively. The levels of gibberellins (GA3)-, indole-3-acetic acid (IAA)-, and abscisic acid (ABA)-immunoreactive equivalents increased by 1.5-, 1.29-, and 1.75-fold. High-throughput 16S rDNA gene and ITS amplicon sequencing further revealed that inoculation reshaped the composition of bacterial and fungal communities in the rhizosphere. Collectively, these findings demonstrate that insect-derived S. marcescens exhibits significant growth-promoting potential in short-cycle leafy vegetable systems, with effects associated with hormone regulation, enhanced total sugar accumulation, and shifts in rhizosphere microbial community structure.<\/jats:p>","DOI":"10.3390\/ijms27073136","type":"journal-article","created":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T08:09:18Z","timestamp":1774944558000},"page":"3136","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Growth-Promoting and Quality-Enhancing Effects of Insect-Derived Serratia marcescens BRC-CXG2 on Romaine Lettuce"],"prefix":"10.3390","volume":"27","author":[{"given":"Xinran","family":"Hu","sequence":"first","affiliation":[{"name":"College of Tea and Food, Wuyi University, Wuyishan 354300, China"},{"name":"State Key Laboratory of Agricultural and Forestry Biosecurity, College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yukun","family":"Zhu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Agricultural and Forestry Biosecurity, College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhao","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Tea and Food, Wuyi University, Wuyishan 354300, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoxi","family":"Ji","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Agricultural and Forestry Biosecurity, College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhitong","family":"Lin","sequence":"additional","affiliation":[{"name":"College of Tea and Food, Wuyi University, Wuyishan 354300, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moyan","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Tea and Food, Wuyi University, Wuyishan 354300, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fen","family":"Li","sequence":"additional","affiliation":[{"name":"College of Tea and Food, Wuyi University, Wuyishan 354300, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaping","family":"Xu","sequence":"additional","affiliation":[{"name":"College of Tea and Food, Wuyi University, Wuyishan 354300, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaiqi","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Tea and Food, Wuyi University, Wuyishan 354300, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenyu","family":"Tian","sequence":"additional","affiliation":[{"name":"College of Tea and Food, Wuyi University, Wuyishan 354300, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaohong","family":"Han","sequence":"additional","affiliation":[{"name":"College of Tea and Food, Wuyi University, Wuyishan 354300, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,3,30]]},"reference":[{"key":"ref_1","first-page":"100421","article-title":"The dual nature of plant growth-promoting bacteria: Benefits, risks, and pathways to sustainable deployment","volume":"9","author":"Etesami","year":"2025","journal-title":"Curr. Res. Microb. Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"e17882","DOI":"10.7717\/peerj.17882","article-title":"Plant growth promoting bacteria (PGPB)-induced plant adaptations to stresses: An updated review","volume":"12","author":"Fania","year":"2024","journal-title":"PeerJ"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"de Andrade, L.A., Santos, C.H.B., Frezarin, E.T., Sales, L.R., and Rigobelo, E.C. (2023). Plant growth-promoting rhizobacteria for sustainable agricultural production. Microorganisms, 11.","DOI":"10.3390\/microorganisms11041088"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Kumar, M., Giri, V.P., Pandey, S., Gupta, A., Patel, M.K., Bajpai, A.B., Jenkins, S., and Siddique, K.H.M. (2021). Plant-growth-promoting rhizobacteria emerging as an effective bioinoculant to improve the growth, production, and stress tolerance of vegetable crops. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms222212245"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1038\/s41598-018-37118-0","article-title":"Pangenome of Serratia marcescens strains from nosocomial and environmental origins reveals different populations and the links between them","volume":"9","author":"Abreo","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Ho, T.M., Le, M.V., Nguyen, H.H.T., Phan, Q., Bui, T.P., Ly, L.K., Lam, V.B., Vandecasteele, M., Goormachtig, S., and Chu, H.H. (2025). Serratia marcescens Strain VIRS2 Isolated from Saline Soil Enhances Rice Growth and Salt Tolerance. Microbiol. Res., 16.","DOI":"10.3390\/microbiolres16050097"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Matteoli, F.P., Passarelli-Araujo, H., Reis, R.J.A., da Rocha, L.O., de Souza, E.M., Aravind, L., Olivares, F.L., and Venancio, T.M. (2018). Genome sequencing and assessment of plant growth-promoting properties of a Serratia marcescens strain isolated from vermicompost. BMC Genom., 19.","DOI":"10.1186\/s12864-018-5130-y"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Gong, A., Wang, G., Sun, Y., Song, M., Dimuna, C., Gao, Z., Wang, H., and Yang, P. (2022). Dual activity of Serratia marcescens Pt-3 in phosphate-solubilizing and production of antifungal volatiles. BMC Microbiol., 22.","DOI":"10.1186\/s12866-021-02434-5"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"815","DOI":"10.1007\/s00203-009-0513-8","article-title":"Characterization of the mineral phosphate solubilizing activity of Serratia marcescens CTM 50650 isolated from the phosphate mine of Gafsa","volume":"191","author":"Farhat","year":"2009","journal-title":"Arch. Microbiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"65834","DOI":"10.1007\/s11356-024-35554-2","article-title":"Survey and genomic characterization of Serratia marcescens on endophytism, biofilm, and phosphorus solubilization in rice plants","volume":"31","author":"Ajulo","year":"2024","journal-title":"Environ. Sci. Pollut. Res. Int."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"114459","DOI":"10.1016\/j.scienta.2025.114459","article-title":"Metabolite-mediated microbial community modulation by Serratia marcescens enhances cucumber seedling growth in artificial cultivation systems","volume":"352","author":"Feng","year":"2025","journal-title":"Sci. Hortic."},{"key":"ref_12","first-page":"555814","article-title":"Serratia A Novel Source of Secondary Metabolites","volume":"11","year":"2018","journal-title":"Adv. Biotechnol. Microbiol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/j.hjb.2016.12.006","article-title":"Plant growth-promoting endophyte Serratia marcescens AL2-16 enhances the growth of Achyranthes aspera L., a medicinal plant","volume":"23","author":"Khaidem","year":"2016","journal-title":"Hayati J. Biosci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1007\/s11274-012-1163-6","article-title":"Multifarious beneficial traits and plant growth promoting potential of Serratia marcescens KiSII and Enterobacter sp. RNF 267 isolated from the rhizosphere of coconut palms (Cocos nucifera L.)","volume":"29","author":"George","year":"2013","journal-title":"World J. Microbiol. Biotechnol."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Zheng, J., Liu, C., Liu, J., and Zhuang, J.Y. (2022). Study of the effect of bacterial-mediated legume plant growth using bacterial strain Serratia marcescens N1.14 X-45. Front. Microbiol., 13.","DOI":"10.3389\/fmicb.2022.988692"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1007\/s11274-025-04288-9","article-title":"The multifaceted roles of gut microbiota in insect physiology, metabolism, and environmental adaptation: Implications for pest management strategies","volume":"41","author":"Haider","year":"2025","journal-title":"World J. Microbiol. Biotechnol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1038\/s41522-023-00435-y","article-title":"The pivotal roles of gut microbiota in insect plant interactions for sustainable pest management","volume":"9","author":"Zhang","year":"2023","journal-title":"npj Biofilms Microbiomes"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"126956","DOI":"10.1016\/j.micres.2021.126956","article-title":"The endophytic bacterial entomopathogen Serratia marcescens promotes plant growth and improves resistance against Nilaparvata lugens in rice","volume":"256","author":"Niu","year":"2022","journal-title":"Microbiol. Res."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Li, Q., Gao, H., Zhang, X., Ni, J., and Mao, H. (2022). Describing Lettuce Growth Using Morphological Features Combined with Nonlinear Models. Agronomy, 12.","DOI":"10.3390\/agronomy12040860"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"3711","DOI":"10.1093\/jxb\/erac074","article-title":"Serratia marcescens PLR enhances lateral root formation through supplying PLR-derived auxin and enhancing auxin biosynthesis in Arabidopsis thaliana","volume":"73","author":"Zhang","year":"2022","journal-title":"J. Exp. Bot."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Zhang, X., Peng, J., Hao, X., Feng, G., Shen, Y., Wang, G., and Chen, Z. (2024). Serratia marcescens LYGN1 reforms the rhizosphere microbial community and promotes cucumber and pepper growth in plug seedling cultivation. Plants, 13.","DOI":"10.3390\/plants13050592"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"6804","DOI":"10.1073\/pnas.1800442115","article-title":"Effect of environmental changes on vegetable and legume yields and nutritional quality","volume":"115","author":"Scheelbeek","year":"2018","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"25994","DOI":"10.1038\/s41598-025-09671-y","article-title":"Effects of different combinations of organic fertilizers on the yield and quality of four leafy vegetables","volume":"15","author":"Liu","year":"2025","journal-title":"Sci. Rep."},{"key":"ref_24","first-page":"1457","article-title":"Plant growth-promoting rhizobacteria Bacillus velezensis JB0319 promotes lettuce growth under salt stress by modulating plant physiology and changing the rhizosphere bacterial community","volume":"29","author":"Bai","year":"2023","journal-title":"Physiol. Mol. Biol. Plants"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"104969","DOI":"10.1016\/j.apsoil.2023.104969","article-title":"Lettuce growth improvement by Azospirillum argentinense and fluorescent Pseudomonas co-inoculation depends on strain compatibility","volume":"189","author":"Merlo","year":"2023","journal-title":"Appl. Soil Ecol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1590\/S1517-83822008000300003","article-title":"Comparison of plant growth-promotion with Pseudomonas aeruginosa and Bacillus subtilis in three vegetables","volume":"39","author":"Adesemoye","year":"2008","journal-title":"Braz. J. Microbiol."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Oliveira, C.E.D.S., Sena Oliveira, T.J.S., Jalal, A., Fernandes, G.C., Bastos, A.C., da Silva, M.R., Sant\u2019Ana, G.R., Aguilar, J.V., de Camargos, L.S., and Zoz, T. (2025). Inoculation and co-inoculation of lettuce and arugula hydroponically influence nitrogen metabolism, plant growth, nutrient acquisition and photosynthesis. Front. Plant Sci., 16.","DOI":"10.3389\/fpls.2025.1547821"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Aloo, B.N., Dessureault-Rompr\u00e9, J., Tripathi, V., Nyongesa, B.O., and Were, B.A. (2023). Signaling and crosstalk of rhizobacterial and plant hormones that mediate abiotic stress tolerance in plants. Front. Microbiol., 14.","DOI":"10.3389\/fmicb.2023.1171104"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"883","DOI":"10.1093\/aob\/mcl027","article-title":"Role of cytokinin and auxin in shaping root architecture: Regulating vascular differentiation, lateral root initiation, root apical dominance and root gravitropism","volume":"97","author":"Aloni","year":"2006","journal-title":"Ann. Bot."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Castro-Camba, R., S\u00e1nchez, C., Vidal, N., and Vielba, J.M. (2022). Plant development and crop yield: The role of gibberellins. Plants, 11.","DOI":"10.3390\/plants11192650"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"740","DOI":"10.1038\/nature01387","article-title":"Auxin promotes Arabidopsis root growth by modulating gibberellin response","volume":"421","author":"Fu","year":"2003","journal-title":"Nature"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Khan, N. (2025). Molecular insights into ABA-mediated regulation of stress tolerance and development in plants. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms26167872"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Kempa, S., Krasensky, J., Dal Santo, S., Kopka, J., and Jonak, C. (2008). A central role of abscisic acid in stress-regulated carbohydrate metabolism. PLoS ONE, 3.","DOI":"10.1371\/journal.pone.0003935"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Guo, J., Beemster, G.T.S., Liu, F., Wang, Z., and Li, X. (2023). Abscisic acid regulates carbohydrate metabolism, redox homeostasis and hormonal regulation to enhance cold tolerance in spring barley. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms241411348"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Zhao, S.Y., Meng, Y.L., Yang, Z.H., Li, B.L., Li, Y.Y., Han, H., Liu, L., Duan, P.F., and Chen, Z.J. (2025). Rhizosphere microbiome metagenomics in PGPR-mediated alleviation of combined stress from polypropylene microplastics and Cd in hybrid Pennisetum. Front. Microbiol., 16.","DOI":"10.3389\/fmicb.2025.1549043"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"fiae088","DOI":"10.1093\/femsec\/fiae088","article-title":"The rhizosphere microbiome of 51 potato cultivars with diverse plant growth characteristics","volume":"100","author":"Martins","year":"2024","journal-title":"FEMS Microbiol. Ecol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/j.foodchem.2016.12.052","article-title":"Comparison between titrimetric and spectrophotometric methods for quantification of vitamin C","volume":"224","author":"Mazalli","year":"2017","journal-title":"Food Chem."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"508","DOI":"10.1042\/bj0570508","article-title":"The estimation of carbohydrates in plant extracts by anthrone","volume":"57","author":"Yemm","year":"1954","journal-title":"Biochem. J."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Chen, R., Ren, L., Yang, G., Cheng, Z., Zhao, D., Zhang, C., Feng, H., Hu, H., and Yang, H. (2025). Estimation of leaf chlorophyll content of maize from hyperspectral data using e2d-cos feature selection, deep neural network, and transfer learning. Agriculture, 15.","DOI":"10.3390\/agriculture15101072"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/7\/3136\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T09:26:45Z","timestamp":1775122005000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/7\/3136"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,30]]},"references-count":39,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2026,4]]}},"alternative-id":["ijms27073136"],"URL":"https:\/\/doi.org\/10.3390\/ijms27073136","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,30]]}}}