{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T18:11:36Z","timestamp":1792174296913,"version":"4.4.0"},"reference-count":47,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T00:00:00Z","timestamp":1774915200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82204094"],"award-info":[{"award-number":["82204094"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Microplastics and nanoplastics (MPs\/NPs) have emerged as pervasive and persistent environmental contaminants, prompting significant concerns about their potential risks to human health. This review provides a comprehensive synthesis of the current state of knowledge on the reproductive toxicity induced by MPs\/NPs, with a particular focus on nanoplastics (NPs, &lt;100 nm) due to their enhanced ability to cross biological barriers and induce cellular damage. Following a systematic literature search, we detail the multiple exposure pathways\u2014including ingestion, inhalation, and dermal contact\u2014through which MPs\/NPs enter the human body and are disseminated to reproductive tissues. The core of this review elucidates the fundamental mechanisms underlying MPs\/NPs-induced reproductive damage. Compelling evidence from in vitro, animal, and initial human studies demonstrates that MP\/NP exposure can lead to diminished sperm quality and motility, testicular histological disruption, impaired ovarian folliculogenesis, granulosa cell apoptosis, and dysregulation of key reproductive hormones. We further summarize potential therapeutic interventions, such as antioxidants and traditional Chinese medicine compounds, and discuss key preventive and regulatory strategies. Despite the advancing evidence, critical challenges remain, including quantifying actual human exposure levels, understanding the effects of chronic, low-dose exposure, and elucidating the combined toxicity of MPs\/NPs with other environmental pollutants. This comprehensive analysis underscores the urgent need for further mechanistic research, robust epidemiological studies, and the formulation of evidence-based public health policies to mitigate exposure and safeguard global reproductive health.<\/jats:p>","DOI":"10.3390\/ijms27073191","type":"journal-article","created":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T16:34:08Z","timestamp":1774974848000},"page":"3191","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Advances in Male Reproductive Toxicology of Nanoplastics: Potential Risks to Human Reproduction\u2014A Systematic Review"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-6861-4987","authenticated-orcid":false,"given":"Hang","family":"Han","sequence":"first","affiliation":[{"name":"Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, School of Basic Medical Sciences, Ningxia Medical University, Yinchuan 750004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Xu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, School of Basic Medical Sciences, Ningxia Medical University, Yinchuan 750004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiuying","family":"Pei","sequence":"additional","affiliation":[{"name":"Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, School of Basic Medical Sciences, Ningxia Medical University, Yinchuan 750004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4101-1000","authenticated-orcid":false,"given":"Xufeng","family":"Fu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, School of Basic Medical Sciences, Ningxia Medical University, Yinchuan 750004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,3,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1016\/j.envpol.2019.05.102","article-title":"Recent advances in toxicological research of nanoplastics in the environment: A review","volume":"252","author":"Shen","year":"2019","journal-title":"Environ. Pollut."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1146\/annurev.publhealth.012809.103714","article-title":"Plastics and health risks","volume":"31","author":"Halden","year":"2010","journal-title":"Annu. Rev. Public Health"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"e1700782","DOI":"10.1126\/sciadv.1700782","article-title":"Production, use, and fate of all plastics ever made","volume":"3","author":"Geyer","year":"2017","journal-title":"Sci. Adv."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1455","DOI":"10.1126\/science.aba9475","article-title":"Evaluating scenarios toward zero plastic pollution","volume":"369","author":"Lau","year":"2020","journal-title":"Science"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"7760","DOI":"10.1021\/acs.est.0c02178","article-title":"COVID-19 pandemic repercussions on the use and management of plastics","volume":"54","author":"Prata","year":"2020","journal-title":"Environ. Sci. Technol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"140279","DOI":"10.1016\/j.scitotenv.2020.140279","article-title":"COVID-19 face masks: A potential source of microplastic fibers in the environment","volume":"737","author":"Fadare","year":"2020","journal-title":"Sci. Total Environ."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.envpol.2017.11.043","article-title":"Airborne microplastics: Consequences to human health?","volume":"234","author":"Prata","year":"2018","journal-title":"Environ. Pollut."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"12300","DOI":"10.1021\/acs.est.9b02540","article-title":"Plastic teabags release billions of microparticles and nanoparticles into tea","volume":"53","author":"Hernandez","year":"2019","journal-title":"Environ. Sci. Technol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1016\/j.watres.2019.03.091","article-title":"Exposure to microplastics (<10 \u03bcm) associated to plastic bottles mineral water consumption: The first quantitative study","volume":"157","author":"Zuccarello","year":"2019","journal-title":"Water Res."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"24131","DOI":"10.1021\/acs.est.5c05922","article-title":"The Human Plastiphere: A Bioparticulate System Challenging Microplastic Risk Assessment and Governance","volume":"59","author":"Shruti","year":"2025","journal-title":"Environ. Sci. Technol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"107199","DOI":"10.1016\/j.envint.2022.107199","article-title":"Discovery and quantification of plastic particle pollution in human blood","volume":"163","author":"Leslie","year":"2022","journal-title":"Environ. Int."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"106274","DOI":"10.1016\/j.envint.2020.106274","article-title":"Plasticenta: First evidence of microplastics in human placenta","volume":"146","author":"Ragusa","year":"2021","journal-title":"Environ. Int."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.maturitas.2018.06.010","article-title":"Plastic contamination of the food chain: A threat to human health?","volume":"115","author":"Waring","year":"2018","journal-title":"Maturitas"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"8932","DOI":"10.1021\/acs.est.5b01090","article-title":"Potential health impact of environmentally released micro- and nanoplastics in the human food production chain: Experiences from nanotoxicology","volume":"49","author":"Bouwmeester","year":"2015","journal-title":"Environ. Sci. Technol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1006\/taap.2001.9240","article-title":"Size-dependent proinflammatory effects of ultrafine polystyrene particles: A role for surface area and oxidative stress in the enhanced activity of ultrafines","volume":"175","author":"Brown","year":"2001","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/j.tiv.2015.11.006","article-title":"Polystyrene nanoparticles internalization in human gastric adenocarcinoma cells","volume":"31","author":"Forte","year":"2016","journal-title":"Toxicol. In Vitro"},{"key":"ref_17","unstructured":"(2016). EFSA Panel on Contaminants in the Food Chain. Presence of microplastics and nanoplastics in food, with particular focus on seafood. Efsa J., 14, e4501."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1312","DOI":"10.1016\/j.scitotenv.2017.07.144","article-title":"Enhanced uptake of bpa in the presence of nanoplastics can lead to neurotoxic effects in adult zebrafish","volume":"609","author":"Chen","year":"2017","journal-title":"Sci. Total Environ."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Yong, C.Q.Y., Valiyaveettil, S., and Tang, B.L. (2020). Toxicity of microplastics and nanoplastics in mammalian systems. Int. J. Environ. Res. Public Health, 17.","DOI":"10.3390\/ijerph17051509"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/j.biomaterials.2016.01.064","article-title":"Carboxyl- and amino-functionalized polystyrene nanoparticles differentially affect the polarization profile of M1 and M2 macrophage subsets","volume":"85","author":"Fuchs","year":"2016","journal-title":"Biomaterials"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"166258","DOI":"10.1016\/j.scitotenv.2023.166258","article-title":"Adverse effects of microplastics and nanoplastics on the reproductive system: A comprehensive review of fertility and potential harmful interactions","volume":"903","author":"Hong","year":"2023","journal-title":"Sci. Total Environ."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"171299","DOI":"10.1016\/j.scitotenv.2024.171299","article-title":"Comparing the effects and mechanisms of exposure to polystyrene nanoplastics with different functional groups on the male reproductive system","volume":"922","author":"Gao","year":"2024","journal-title":"Sci. Total Environ."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"139138","DOI":"10.1016\/j.chemosphere.2023.139138","article-title":"Effects of micro(nano)plastics on the reproductive system: A review","volume":"336","author":"Song","year":"2023","journal-title":"Chemosphere"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2881","DOI":"10.1002\/tox.23923","article-title":"Polystyrene nanoplastics aggravate reproductive system damage in obese male mice by perturbation of the testis redox homeostasis","volume":"38","author":"Cai","year":"2023","journal-title":"Environ. Toxicol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"3993","DOI":"10.1007\/s10815-025-03644-w","article-title":"Kisspeptin as a marker for male infertility: A comparative study of serum and seminal plasma kisspeptin between fertile and infertile men","volume":"42","author":"Parkpinyo","year":"2025","journal-title":"J. Assist. Reprod. Genet."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Hu, Y., Jiang, S., Xu, Y., Zhang, Y., Zhang, Q., Zhou, W., Liang, J., and Su, W. (2025). Comparative analysis of reproductive toxicity of polystyrene-nanoplastics and polystyrene-microplastics in rat Sertoli cells. Andrology.","DOI":"10.1111\/andr.70115"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"107220","DOI":"10.1016\/j.envint.2022.107220","article-title":"Repression of autophagy leads to acrosome biogenesis disruption caused by a sub-chronic oral administration of polystyrene nanoparticles","volume":"163","author":"Zhou","year":"2022","journal-title":"Environ. Int."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.reprotox.2021.10.002","article-title":"Polystyrene microplastics disturb maternal-fetal immune balance and cause reproductive toxicity in pregnant mice","volume":"106","author":"Hu","year":"2021","journal-title":"Reprod. Toxicol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"23","DOI":"10.5334\/aogh.4056","article-title":"The Minderoo-Monaco Commission on Plastics and Human Health","volume":"89","author":"Landrigan","year":"2023","journal-title":"Ann. Glob. Health"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"151","DOI":"10.5334\/aogh.2831","article-title":"Human Health and Ocean Pollution","volume":"86","author":"Landrigan","year":"2020","journal-title":"Ann. Glob. Health"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"e2300582121","DOI":"10.1073\/pnas.2300582121","article-title":"Rapid single-particle chemical imaging of nanoplastics by SRS microscopy","volume":"121","author":"Qian","year":"2024","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"122947","DOI":"10.1016\/j.envres.2025.122947","article-title":"Associations between PM2.5 components, temperature, and semen quality: Insights from seminal plasma metabolome","volume":"286","author":"Tan","year":"2025","journal-title":"Environ. Res."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1631\/jzus.B2300138","article-title":"Nrf2-mediated ferroptosis of spermatogenic cells involved in male reproductive toxicity induced by polystyrene nanoplastics in mice","volume":"25","author":"Fu","year":"2024","journal-title":"J. Zhejiang Univ. B"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"105893","DOI":"10.1016\/j.tiv.2024.105893","article-title":"Integrated transcriptomic and metabolomic analysis reveals the underlying mechanisms for male reproductive toxicity of polystyrene nanoplastics in mouse spermatocyte-derived GC-2spd(ts) cells","volume":"100","author":"Han","year":"2024","journal-title":"Toxicol. In Vitro"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1277","DOI":"10.1002\/tox.23763","article-title":"Integrated fecal microbiome and metabolome analysis explore the link between polystyrene nanoplastics exposure and male reproductive toxicity in mice","volume":"38","author":"Fu","year":"2023","journal-title":"Environ. Toxicol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"154429","DOI":"10.1016\/j.tox.2026.154429","article-title":"Polystyrene nanoplastics disrupt iron homeostasis by promoting FPN1 ubiquitination in GC-2spd(ts) cells","volume":"523","author":"Cui","year":"2026","journal-title":"Toxicology"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"162189","DOI":"10.1016\/j.scitotenv.2023.162189","article-title":"Paeoniflorin attenuates polystyrene nanoparticle-induced reduction in reproductive capacity and increase in germline apoptosis through suppressing DNA damage checkpoints in Caenorhabditis elegans","volume":"871","author":"Hua","year":"2023","journal-title":"Sci. Total Environ."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"119979","DOI":"10.1016\/j.envres.2024.119979","article-title":"Research progress on degradation of biodegradable micro-nano plastics and its toxic effect mechanism on soil ecosystem","volume":"262","author":"Liu","year":"2024","journal-title":"Environ. Res."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"2873","DOI":"10.1038\/s41591-025-03902-5","article-title":"Health impacts of microplastic and nanoplastic exposure","volume":"31","author":"Lamoree","year":"2025","journal-title":"Nat. Med."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Bazeli, J., Banikazemi, Z., Hamblin, M.R., and Chaleshtori, R.S. (2023). Could probiotics protect against human toxicity caused by polystyrene nanoplastics and microplastics?. Front. Nutr., 10.","DOI":"10.3389\/fnut.2023.1186724"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Zhang, X., Wu, Y., Fu, X., He, S., Shi, L., Xu, H., Shi, X., Yang, Y., Zhu, Y., and Wang, Y. (2024). Toxicity to the Male Reproductive System after Exposure to Polystyrene Nanoplastics: A Macrogenomic and Metabolomic Analysis. Toxics, 12.","DOI":"10.3390\/toxics12080531"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"115618","DOI":"10.1016\/j.ecoenv.2023.115618","article-title":"The male reproductive toxicity after nanoplastics and microplastics exposure: Sperm quality and changes of different cells in testis","volume":"267","author":"Gao","year":"2023","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Tolardo, V., Magr\u00ec, D., Fumagalli, F., Cassano, D., Athanassiou, A., Fragouli, D., and Gioria, S. (2022). In Vitro High-Throughput Toxicological Assessment of Nanoplastics. Nanomaterials, 12.","DOI":"10.3390\/nano12121947"},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Wu, B., Yu, H., Yi, J., Lei, P., He, J., Ruan, J., Xu, P., Tao, R., Jin, L., and Wu, W. (2024). Behavioral Studies of Zebrafish Reveal a New Perspective on the Reproductive Toxicity of Micro- and Nanoplastics. Toxics, 12.","DOI":"10.3390\/toxics12030178"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"117736","DOI":"10.1016\/j.ecoenv.2025.117736","article-title":"Exposure to nano-polystyrene during pregnancy leads to Alzheimer\u2019s disease-related pathological changes in adult offspring","volume":"290","author":"Wang","year":"2025","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Liu, Y., Hao, F., Liang, H., Liu, W., and Guo, Y. (2025). Exposure to polystyrene nanoplastics impairs sperm metabolism and pre-implantation embryo development in mice. Front. Cell Dev. Biol., 13.","DOI":"10.3389\/fcell.2025.1562331"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"5589","DOI":"10.1021\/acsnano.4c15112","article-title":"Polylactic Acid Micro\/Nanoplastic Exposure Induces Male Reproductive Toxicity by Disrupting Spermatogenesis and Mitochondrial Dysfunction in Mice","volume":"19","author":"Zhao","year":"2025","journal-title":"ACS Nano"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/7\/3191\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T04:10:39Z","timestamp":1775189439000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/7\/3191"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,31]]},"references-count":47,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2026,4]]}},"alternative-id":["ijms27073191"],"URL":"https:\/\/doi.org\/10.3390\/ijms27073191","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,31]]}}}