{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T14:28:16Z","timestamp":1785421696708,"version":"3.56.0"},"reference-count":39,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Institute of Allergy and Infectious Diseases interagency agreement","award":["AAI26003-001-00000"],"award-info":[{"award-number":["AAI26003-001-00000"]}]},{"award":["AAI26003-001-00000"],"award-info":[{"award-number":["AAI26003-001-00000"]}],"id":[{"id":"https:\/\/ror.org\/043z4tv69","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Opportunity Funds Management Core of the Centers for Medical Countermeasures against Radiation, National Institute of Allergy and Infectious Diseases","award":["U19AI067773"],"award-info":[{"award-number":["U19AI067773"]}]},{"award":["U19AI067773"],"award-info":[{"award-number":["U19AI067773"]}],"id":[{"id":"https:\/\/ror.org\/043z4tv69","id-type":"ROR","asserted-by":"publisher"}]},{"name":"AFRRI intramural","award":["AFR-B2-11616"],"award-info":[{"award-number":["AFR-B2-11616"]}]},{"name":"AFRRI intramural","award":["AFR-B2-15755"],"award-info":[{"award-number":["AFR-B2-15755"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Accurate radiation biodosimetry is urgently needed for medical management after large-scale radiation exposure. Partial-body irradiation with 5% bone marrow sparing (PBI\/BM5) provides a realistic radiation model. The current study specifically focused on the high-dose lethality window (12\u201316 Gy), where survival transitioned from 100% to 0%, representing a clinically distinct and underserved scenario requiring dedicated biodosimetry tools. We defined the survival profile of male C57BL\/6 mice after PBI\/BM5 and found that doses of 13.5\u201314.0 Gy were nonlethal within 12 days, whereas 15.0\u201315.5 Gy caused 100% mortality within 12 days, with a calculated LD50\/12 of 14.68 Gy. A separate cohort of 14.0 Gy showed 100% survival up to 90 days post-radiation. To develop serum cytokine-based biodosimetry in high-dose radiation exposure, mice were exposed to 12.0\u201316.0 Gy PBI\/BM5, and serum was collected on days 1, 3, and 7. A multiplex cytokine assay was used to quantify 70 total cytokines\/chemokines. After the exclusion of 4 targets outside detection limits, 66 markers were utilized for downstream analysis. PCA, clustering and heatmaps, and LASSO classification revealed that cytokine signatures can classify radiation groups\/status\/doses. A 4-cytokine panel (IL-7, GDF-15, IL-16 and FLT3L) could distinguish na\u00efve vs. irradiated mice on all study days. A 24-cytokine signature panel distinguished radiation survivors vs. non-survivors, and another 34-cytokine panel separated radiation doses (12\u201316 Gy); the prediction was better on day 7 compared to earlier time points. This exploratory study was specifically designed to define the systemic inflammatory response in a high-dose window where survival transitions from 100% to 0% (the \u2018lethality threshold\u2019) in a clinically relevant partial-body irradiation model. These findings show that serum cytokines have strong potential for high-dose triage, survival prediction, and dose discrimination within the near-lethal exposure range in a clinically relevant PBI\/BM5 model. Extension to lower dose ranges is an important direction for future work.<\/jats:p>","DOI":"10.3390\/ijms27073213","type":"journal-article","created":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T07:57:55Z","timestamp":1775116675000},"page":"3213","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Cytokine Profiling for the Prediction of Lethality and High-Dose Exposure in a Murine Partial Body Irradiation Model"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6408-8601","authenticated-orcid":false,"given":"Wanchang","family":"Cui","sequence":"first","affiliation":[{"name":"Armed Forces Radiobiology Research Institute, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA"},{"name":"Department of Pharmacology and Molecular Therapeutics, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa","family":"Hull","sequence":"additional","affiliation":[{"name":"Armed Forces Radiobiology Research Institute, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA"},{"name":"Henry M Jackson Foundation for the Advancement of Military Medicine, Inc., Bethesda, MD 20817, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asher","family":"Rothstein","sequence":"additional","affiliation":[{"name":"Armed Forces Radiobiology Research Institute, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA"},{"name":"Henry M Jackson Foundation for the Advancement of Military Medicine, Inc., Bethesda, MD 20817, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4700-989X","authenticated-orcid":false,"given":"Li","family":"Wang","sequence":"additional","affiliation":[{"name":"Armed Forces Radiobiology Research Institute, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA"},{"name":"Henry M Jackson Foundation for the Advancement of Military Medicine, Inc., Bethesda, MD 20817, USA"},{"name":"Department of Pathology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Lin","sequence":"additional","affiliation":[{"name":"Armed Forces Radiobiology Research Institute, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA"},{"name":"Henry M Jackson Foundation for the Advancement of Military Medicine, Inc., Bethesda, MD 20817, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Zhai","sequence":"additional","affiliation":[{"name":"Armed Forces Radiobiology Research Institute, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA"},{"name":"Henry M Jackson Foundation for the Advancement of Military Medicine, Inc., Bethesda, MD 20817, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alia","family":"Weaver","sequence":"additional","affiliation":[{"name":"Armed Forces Radiobiology Research Institute, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mang","family":"Xiao","sequence":"additional","affiliation":[{"name":"Armed Forces Radiobiology Research Institute, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,4,1]]},"reference":[{"key":"ref_1","first-page":"514","article-title":"Development of Biomarkers for Radiation Biodosimetry and Medical Countermeasures Research: Current Status, Utility, and Regulatory Pathways","volume":"197","author":"Satyamitra","year":"2022","journal-title":"Radiat. 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