{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T07:16:42Z","timestamp":1789888602536,"version":"4.0.1"},"reference-count":36,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,4,12]],"date-time":"2026-04-12T00:00:00Z","timestamp":1775952000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Oral biofilms are complex polymicrobial communities involved in the development of dental caries and periodontal diseases. Chemical antiseptics are commonly used as adjuncts to mechanical plaque control; however, their antimicrobial efficacy varies depending on composition and mechanism of action. The aim of this study was to comparatively evaluate the antimicrobial and antibiofilm activities of four commercially available dental products (Corsodyl, Ozosan, HybenX, and Elugel) against a broad spectrum of oral microorganisms. This in vitro study included Gram-positive and Gram-negative bacterial strains, comprising both reference strains and clinical isolates, as well as Candida albicans. Antimicrobial activity was assessed using the disc diffusion assay, while antibiofilm activity was evaluated using a crystal violet microplate assay. All experiments were performed in triplicate. Statistical analysis was conducted using two-way ANOVA followed by Tukey\u2019s post hoc test (p &lt; 0.05). All tested products exhibited antimicrobial activity. Inhibition zones ranged from 9 to 56 mm for Gram-positive bacteria, 12 to 38 mm for Gram-negative bacteria, and 13 to 43 mm for Candida albicans. Two-way ANOVA revealed a significant effect of the dental product (p &lt; 0.001), while incubation time was not significant (p &gt; 0.05). HybenX showed the highest antimicrobial efficacy, while chlorhexidine-based products demonstrated consistent activity. Antibiofilm inhibition exceeded 80% for several strains. Dental antiseptics exhibit significantly different antimicrobial and antibiofilm profiles, highlighting the importance of appropriate product selection in oral biofilm control.<\/jats:p>","DOI":"10.3390\/ijms27083450","type":"journal-article","created":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T08:49:02Z","timestamp":1776070142000},"page":"3450","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Targeting Oral Biofilms: Comparative In Vitro Evaluation of Commercial Dental Antiseptics Against Clinical and Reference Microbial Strains"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-1686-0150","authenticated-orcid":false,"given":"Vanessa","family":"Bolchis","sequence":"first","affiliation":[{"name":"Translational and Experimental Clinical Research Centre in Oral Health, Faculty of Dental Medicine, \u201cVictor Babes\u201d University of Medicine and Pharmacy Timisoara, 300041 Timisoara, Romania"},{"name":"Clinic of Preventive, Community Dentistry and Oral Health, Faculty of Dental Medicine, \u201cVictor Babes\u201d University of Medicine and Pharmacy Timisoara, 300041 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-7604-7708","authenticated-orcid":false,"given":"Delia","family":"Abrudan-Luca","sequence":"additional","affiliation":[{"name":"Translational and Experimental Clinical Research Centre in Oral Health, Faculty of Dental Medicine, \u201cVictor Babes\u201d University of Medicine and Pharmacy Timisoara, 300041 Timisoara, Romania"},{"name":"Clinic of Preventive, Community Dentistry and Oral Health, Faculty of Dental Medicine, \u201cVictor Babes\u201d University of Medicine and Pharmacy Timisoara, 300041 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6548-9607","authenticated-orcid":false,"given":"Ramona","family":"Dumitrescu","sequence":"additional","affiliation":[{"name":"Translational and Experimental Clinical Research Centre in Oral Health, Faculty of Dental Medicine, \u201cVictor Babes\u201d University of Medicine and Pharmacy Timisoara, 300041 Timisoara, Romania"},{"name":"Clinic of Preventive, Community Dentistry and Oral Health, Faculty of Dental Medicine, \u201cVictor Babes\u201d University of Medicine and Pharmacy Timisoara, 300041 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atena","family":"Galuscan","sequence":"additional","affiliation":[{"name":"Translational and Experimental Clinical Research Centre in Oral Health, Faculty of Dental Medicine, \u201cVictor Babes\u201d University of Medicine and Pharmacy Timisoara, 300041 Timisoara, Romania"},{"name":"Clinic of Preventive, Community Dentistry and Oral Health, Faculty of Dental Medicine, \u201cVictor Babes\u201d University of Medicine and Pharmacy Timisoara, 300041 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marioara Nicoleta","family":"Caraba","sequence":"additional","affiliation":[{"name":"Department of Cellular and Molecular Biology, Faculty of Medicine, \u201cVictor Babe\u0219\u201d University of Medicine and Pharmacy Timisoara, 300041 Timisoara, Romania"},{"name":"ANAPATMOL Research Center, \u201cVictor Babe\u0219\u201d University of Medicine and Pharmacy Timisoara, 300041 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ion Valeriu","family":"Caraba","sequence":"additional","affiliation":[{"name":"ANAPATMOL Research Center, \u201cVictor Babe\u0219\u201d University of Medicine and Pharmacy Timisoara, 300041 Timisoara, Romania"},{"name":"Faculty of Bioengineering of Animal Resources, University of Life Sciences \u201cKing Mihai I\u201d from Timisoara, 119 Calea Aradului, 300645 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9387-1141","authenticated-orcid":false,"given":"Roxana","family":"Popescu","sequence":"additional","affiliation":[{"name":"ANAPATMOL Research Center, \u201cVictor Babe\u0219\u201d University of Medicine and Pharmacy Timisoara, 300041 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mihaela Adina","family":"Dumitrache","sequence":"additional","affiliation":[{"name":"Oral Health and Community Dentistry Department, Faculty of Dental Medicine, \u201cCarol Davila\u201d University of Medicine and Pharmacy, 17-21 Calea Plevnei Street, Sector 1, 010221 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1417-5963","authenticated-orcid":false,"given":"Gabriela","family":"Ciavoi","sequence":"additional","affiliation":[{"name":"Department of Dental Medicine, Faculty of Medicine and Pharmacy, University of Oradea, 410073 Oradea, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2705-5020","authenticated-orcid":false,"given":"Daniela","family":"Jumanca","sequence":"additional","affiliation":[{"name":"Translational and Experimental Clinical Research Centre in Oral Health, Faculty of Dental Medicine, \u201cVictor Babes\u201d University of Medicine and Pharmacy Timisoara, 300041 Timisoara, Romania"},{"name":"Clinic of Preventive, Community Dentistry and Oral Health, Faculty of Dental Medicine, \u201cVictor Babes\u201d University of Medicine and Pharmacy Timisoara, 300041 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,4,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1111\/prd.12361","article-title":"The Dental Plaque Biofilm Matrix","volume":"86","author":"Jakubovics","year":"2021","journal-title":"Periodontology 2000"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"883","DOI":"10.1111\/jcpe.13295","article-title":"Adjunctive Effect of Mouthrinse on Treatment of Peri-implant Mucositis Using Mechanical Debridement: A Randomized Clinical Trial","volume":"47","author":"Philip","year":"2020","journal-title":"J. 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