{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T12:15:43Z","timestamp":1776341743871,"version":"3.51.2"},"reference-count":38,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T00:00:00Z","timestamp":1776297600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"State Program of the Sirius Federal Territory \u201cScientific and technological development of the Sirius Federal Territory\u201d","award":["18-03"],"award-info":[{"award-number":["18-03"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The central role of YB-1 in messenger ribonucleoprotein particle (mRNP) metabolism and stress-granule biology highlights the importance of defining the determinants of its self-assembly. YB-1 fibrillogenesis has been attributed primarily to the cold shock domain (CSD). Here, we show that the YB-1 fragment spanning residues 1\u2013129 (AP\u2013CSD) form amyloid fibrils under near-physiological ionic strength (0.12\u20130.15 M KCl). Fibrillization proceeds without a pronounced exponential growth phase and increases approximately linearly over 45\u201350 h. Far-UV circular dichroism (CD) and attenuated total reflection Fourier-transform infrared spectroscopy (ATR-FTIR) indicate no substantial change in overall secondary-structure content during aggregation. In parallel, 1H nuclear magnetic resonance (NMR) spectroscopy reveals the depletion of soluble species, and oriented fiber X-ray diffraction displays the hallmark cross-\u03b2 reflections at approximately 4.7 \u00c5 and 10 \u00c5. The prolonged formation time implies an activation barrier that is unlikely to require global refolding. Instead, it may reflect early association events such as dimerization or other local rearrangements required for primary nucleation, followed by consolidation into stable intermolecular contacts. Aggregation that preserves a largely native-like fold while establishing cross-\u03b2 order may reduce recognition by cellular quality-control systems that preferentially target globally unfolded or strongly destabilized states. This provides a plausible framework for how YB-1 derived assemblies could persist under stress and during age-associated proteostasis decline.<\/jats:p>","DOI":"10.3390\/ijms27083553","type":"journal-article","created":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T11:35:42Z","timestamp":1776339342000},"page":"3553","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["YB-1 AP\u2013CSD Forms Cross-\u03b2 Amyloid Fibrils Without Secondary-Structure Conversion In Vitro"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2547-8804","authenticated-orcid":false,"given":"Maria A.","family":"Timchenko","sequence":"first","affiliation":[{"name":"Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino 142290, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3962-1520","authenticated-orcid":false,"given":"Oxana V.","family":"Galzitskaya","sequence":"additional","affiliation":[{"name":"Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino 142290, Russia"},{"name":"Gamaleya Research Center of Epidemiology and Microbiology, Moscow 123098, Russia"},{"name":"Institute of Protein Research, Russian Academy of Sciences, Pushchino 142290, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-2813-442X","authenticated-orcid":false,"given":"Alexander V.","family":"Chulkov","sequence":"additional","affiliation":[{"name":"Department of Fundamental Medicine, Mari State University, Pl. Lenina 1, Yoshkar-Ola 424001, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4926-5654","authenticated-orcid":false,"given":"Ilya V.","family":"Likhachev","sequence":"additional","affiliation":[{"name":"Institute of Protein Research, Russian Academy of Sciences, Pushchino 142290, Russia"},{"name":"Institute of Mathematical Problems of Biology, Russian Academy of Sciences, Branch of the Keldysh Institute of Applied Mathematics, Russian Academy of Sciences, Pushchino 142290, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna V.","family":"Glyakina","sequence":"additional","affiliation":[{"name":"Institute of Protein Research, Russian Academy of Sciences, Pushchino 142290, Russia"},{"name":"Institute of Mathematical Problems of Biology, Russian Academy of Sciences, Branch of the Keldysh Institute of Applied Mathematics, Russian Academy of Sciences, Pushchino 142290, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maxim V.","family":"Molchanov","sequence":"additional","affiliation":[{"name":"Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino 142290, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nikolay V.","family":"Molochkov","sequence":"additional","affiliation":[{"name":"Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino 142290, Russia"},{"name":"Institute of Cell Biophysics, FRC PSCBR, Russian Academy of Sciences, Pushchino 142290, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2212-5640","authenticated-orcid":false,"given":"Nikita V.","family":"Penkov","sequence":"additional","affiliation":[{"name":"Institute of Cell Biophysics, FRC PSCBR, Russian Academy of Sciences, Pushchino 142290, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liya G.","family":"Bobyleva","sequence":"additional","affiliation":[{"name":"Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino 142290, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vitalii A.","family":"Balobanov","sequence":"additional","affiliation":[{"name":"Institute of Protein Research, Russian Academy of Sciences, Pushchino 142290, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0629-4014","authenticated-orcid":false,"given":"Alexander Ye.","family":"Yegorov","sequence":"additional","affiliation":[{"name":"Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino 142290, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3058-5460","authenticated-orcid":false,"given":"Sergey G.","family":"Guryanov","sequence":"additional","affiliation":[{"name":"Institute of Protein Research, Russian Academy of Sciences, Pushchino 142290, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexey D.","family":"Nikulin","sequence":"additional","affiliation":[{"name":"Institute of Protein Research, Russian Academy of Sciences, Pushchino 142290, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dmitry N.","family":"Lyabin","sequence":"additional","affiliation":[{"name":"Institute of Protein Research, Russian Academy of Sciences, Pushchino 142290, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6063-6789","authenticated-orcid":false,"given":"Ivan M.","family":"Vikhlyantsev","sequence":"additional","affiliation":[{"name":"Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino 142290, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander G.","family":"Bobylev","sequence":"additional","affiliation":[{"name":"Scientific Center of Genetics and Life Sciences, Sirius University of Science and Technology, Sirius Federal Territory 354340, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,4,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Mordovkina, D., Lyabin, D.N., Smolin, E.A., Sogorina, E.M., Ovchinnikov, L.P., and Eliseeva, I. 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