{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T18:42:18Z","timestamp":1785436938684,"version":"3.56.0"},"reference-count":47,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T00:00:00Z","timestamp":1776384000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002674","name":"Ministry of Science and Higher Education, Russian Federation","doi-asserted-by":"publisher","award":["01201253310"],"award-info":[{"award-number":["01201253310"]}],"id":[{"id":"10.13039\/501100002674","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002674","name":"Russian Academy of Sciences","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100002674","id-type":"DOI","asserted-by":"crossref"}]},{"id":[{"id":"https:\/\/ror.org\/05qrfxd25","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Oxidative stress occurs when there is an excess of reactive oxygen species (ROS) in the cell, primarily produced by mitochondria. Excess ROS trigger membrane lipid peroxidation (LPO), cause mitochondrial swelling, and release proapoptotic proteins into the cytoplasm, which can lead to apoptosis. It is assumed that antioxidants that reduce excessive ROS formation by mitochondria can increase the body\u2019s resistance to stress factors. We investigated the effects of hypoxia and the antioxidant Ambiol (2-methyl-4-dimethylaminomethylbenzimidazole-5-ol dihydrochloride) on the functional characteristics of mitochondria, which were assessed by measuring lipid peroxidation intensity using spectrofluorimetry, mitochondrial membranes fatty acid composition using chromatography, mitochondrial morphology using atomic force microscopy, and respiration rate using polarography. Injecting mice with Ambiol at a dose of 10\u22126 mol\/kg for 5 days prevented the stress-induced activation of lipid peroxidation, a decrease in the unsaturation index of C18 and C20 fatty acids in mitochondrial membranes, and swelling of these organelles. The drug also increased the efficiency of oxidative phosphorylation during the oxidation of NAD-dependent substrates. Furthermore, Ambiol increased the lifespan of mice by 3.0\u20134.0 times under various types of hypoxia. Ambiol\u2019s ability to maintain initial (control) levels of C18 and C20 unsaturated fatty acids appears to protect against stress-induced mitochondrial dysfunction.<\/jats:p>","DOI":"10.3390\/ijms27083589","type":"journal-article","created":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T14:24:51Z","timestamp":1776435891000},"page":"3589","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Ambiol Prevents Changes in the Functional Characteristics of Mitochondria Under Hypoxia"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3790-1112","authenticated-orcid":false,"given":"Irina V.","family":"Zhigacheva","sequence":"first","affiliation":[{"name":"Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, St. Kosygin, 4, Moscow 119334, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5504-456X","authenticated-orcid":false,"given":"Natalya I.","family":"Krikunova","sequence":"additional","affiliation":[{"name":"Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, St. Kosygin, 4, Moscow 119334, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena M.","family":"Mil","sequence":"additional","affiliation":[{"name":"Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, St. Kosygin, 4, Moscow 119334, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ludmila I.","family":"Matienko","sequence":"additional","affiliation":[{"name":"Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, St. Kosygin, 4, Moscow 119334, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4243-2787","authenticated-orcid":false,"given":"Marina A.","family":"Yakovleva","sequence":"additional","affiliation":[{"name":"Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, St. Kosygin, 4, Moscow 119334, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander N.","family":"Goloshchapov","sequence":"additional","affiliation":[{"name":"Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, St. Kosygin, 4, Moscow 119334, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,4,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2038","DOI":"10.2174\/092986732713200425202016","article-title":"Chemistry and pharmacology of modulators of oxidative stress","volume":"27","author":"Saso","year":"2020","journal-title":"Curr. 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