{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T17:25:53Z","timestamp":1785432353595,"version":"3.56.0"},"reference-count":42,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T00:00:00Z","timestamp":1776384000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001677","name":"INSERM","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100001677","id-type":"DOI","asserted-by":"crossref"}]},{"id":[{"id":"https:\/\/ror.org\/02vjkv261","id-type":"ROR","asserted-by":"publisher"}]},{"name":"W-MedPhys"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Adenomyosis is a benign gynaecological disorder in which endometrial glands and stroma enter the uterine myometrium with varying degrees of spreading. To analyse the presence of developmentally displaced endometrial glands and stroma in the foetal myometrium, a retrospective cohort of 420 foetal uteri, including one monozygotic twin pair, was histopathologically evaluated. The gestational age ranged between 18 and 37 weeks; the clinical characteristics included various foetal malformations with a predominantly normal karyotype, except in one case with trisomy 18. Ectopic endometrial tissue enclosed in the myometrium was discovered in twelve individual foetuses from the cohort (12\/420). The investigation of the histogenetic attributes of the misplaced endometrial tissue in both monozygotic twins\u2019 (MZ) foetal uteri revealed isolated glands and thin channels containing cords of endometrial-type glands penetrating the myometrium. Through immunohistochemistry, low levels of oestrogen receptors (ERs) were detected, whereas a moderate level of progesterone receptor (PR) expression was observed in the ectopic glandular and stromal cell nuclei in all cases. Additionally, the surrounding periglandular component consistently expressed the vimentin and CD10 stromal cell markers, while the myometrial smooth muscle cells revealed the strong expression of both alpha-Smooth Muscle Actin (\u03b1-SMA) and desmin marker proteins.<\/jats:p>","DOI":"10.3390\/ijms27083595","type":"journal-article","created":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T14:24:51Z","timestamp":1776435891000},"page":"3595","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Identification of Misplaced Endometrial Glands and Stroma in the Myometrium of Foetal Uteri Evocative of Developmental Adenomyosis"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0280-3603","authenticated-orcid":false,"given":"Jean","family":"Gogusev","sequence":"first","affiliation":[{"name":"Institut Cochin, INSERM UMR 1016, CNRS 8104, Universit\u00e9 Paris Cit\u00e9, 22 Rue M\u00e9chain, 75014 Paris, France"},{"name":"W-MedPhys, 128 Rue la Bo\u00e9tie, 75008 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7746-5170","authenticated-orcid":false,"given":"Yves","family":"Lepelletier","sequence":"additional","affiliation":[{"name":"W-MedPhys, 128 Rue la Bo\u00e9tie, 75008 Paris, France"},{"name":"Institut Imagine, Universit\u00e9 Paris Cit\u00e9, 24 Boulevard Montparnasse, 75015 Paris, France"},{"name":"INSERM UMR 1163, Laboratory of Cellular and Molecular Basis of Normal Hematopoiesis and Hematological Disorders: Therapeutical Implications, 24 Boulevard Montparnasse, 75015 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas","family":"Lopez","sequence":"additional","affiliation":[{"name":"W-MedPhys, 128 Rue la Bo\u00e9tie, 75008 Paris, France"},{"name":"ENOES\/ENESIA, 62 Rue de Miromesnil, 75008 Paris, France"},{"name":"Unit\u00e9 Mixte de Recherche \u201cInstitut de Physique Th\u00e9orique (IPhT)\u201d CEA-CNRS, UMR 3681, Route de l\u2019Orme des Merisiers, 91191 St Aubin-Gif-sur-Yvette, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0102-9262","authenticated-orcid":false,"given":"Patrick","family":"Barbet","sequence":"additional","affiliation":[{"name":"Service d\u2019Anatomie Pathologique, H\u00f4pital Universitaire Necker-Enfants Maladies, 149 Rue de S\u00e8vres, CEDEX 15, 75743 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-1121-1729","authenticated-orcid":false,"given":"Pierre","family":"Validire","sequence":"additional","affiliation":[{"name":"Service d\u2019Anatomie Pathologique, Institut Mutualiste Montsouris, 42 Bd Jourdan, 75014 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,4,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1093\/humupd\/4.4.312","article-title":"Pathophysiology of adenomyosis","volume":"4","author":"Ferenczy","year":"1998","journal-title":"Hum. Reprod. Update"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1007\/s00404-009-1191-0","article-title":"The pathophysiology of endometriosis and adenomyosis: Tissue injury and repair","volume":"280","author":"Leyendecker","year":"2009","journal-title":"Arch. Gynecol. Obstet."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1055\/s-0040-1716687","article-title":"Adenomyosis; Mechanisms and pathogenesis","volume":"38","author":"Zhai","year":"2020","journal-title":"Semin. Reprod. Med."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2087","DOI":"10.1007\/s43032-020-00361-w","article-title":"Origin and Pathogenic Mechanisms of Uterine Adenomyosis: What is known so far","volume":"28","author":"Stratopoulou","year":"2021","journal-title":"Reprod. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1016\/j.fertnstert.2017.12.030","article-title":"Pathogenesis of uterine adenomyosis: Invagination or metaplasia? Fertil","volume":"109","author":"Donnez","year":"2018","journal-title":"Steril."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1541","DOI":"10.1016\/0002-9378(62)90170-9","article-title":"The elusive adenomyosis of the uterus. Its historical past and its present state of recognition","volume":"83","author":"Emge","year":"1962","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1030","DOI":"10.1177\/1933719112472736","article-title":"M\u00fcllerianosis: Four developmental (embryonic) mullerian diseases","volume":"20","author":"Batt","year":"2013","journal-title":"Reprod. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"746","DOI":"10.1016\/j.fertnstert.2023.03.006","article-title":"Endometriosis and adenomyosis: Shared pathophysiology","volume":"119","author":"Bulun","year":"2023","journal-title":"Fertil. Steril."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"778","DOI":"10.1016\/j.biocel.2010.03.008","article-title":"Endometriosis: New concepts in the pathogenesis","volume":"42","author":"Signorile","year":"2010","journal-title":"Int. J. Biochem. Cell Biol."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Signorile, P.G., Baldi, A., Viceconte, R., and Boccellino, M. (2025). The Role of Adenogenesis Factors in the Pathogenesis of Endometriosis. Int. J. Mol. Sci., 26.","DOI":"10.20944\/preprints202501.2226.v1"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"S105","DOI":"10.1016\/j.ajog.2024.08.043","article-title":"Endometriosis and adenomyosis unveiled through single-cell glasses","volume":"232","author":"Giudice","year":"2025","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Bouquet de Jolini\u00e8re, J., Ayoubi, J.M., Lesec, G., Validire, P., Goguin, A., Gianaroli, L., Dubuisson, J.B., Feki, A., and Gogusev, J. (2012). Identification of Displaced Endometrial Glands and Embryonic Duct Remnants in Female Fetal Reproductive Tract: Possible Pathogenetic Role in Endometriotic and Pelvic Neoplastic Processes. Front. Physiol., 3.","DOI":"10.3389\/fphys.2012.00444"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"939","DOI":"10.1016\/0002-9378(82)90189-2","article-title":"Effects of prenatal exposure of mice to \u201clow dose\u201d dyethylstilbestroland the development of adenomyosis associated with evidence of hyperprolactinemia","volume":"144","author":"Huseby","year":"1982","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Stephens, V.R., Rumph, J.T., Ameli, S., Bruner-Tran, K.L., and Osteen, K.G. (2022). The Potential Relationship Between Environmental Endocrine Disruptor Exposure and the Development of Endometriosis and Adenomyosis. Front. Physiol., 12.","DOI":"10.3389\/fphys.2021.807685"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1016\/j.reprotox.2007.07.006","article-title":"Long-term adverse effects of neonatal exposure to bisphenol A on the murine female reproductive tract","volume":"24","author":"Newbold","year":"2007","journal-title":"Reprod. Toxicol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"572","DOI":"10.1016\/j.fertnstert.2012.06.044","article-title":"The pathophysiology of uterine adenomyosis: An update","volume":"98","author":"Benagiano","year":"2012","journal-title":"Fertil. Steril."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Habiba, M., and Benagiano, G. (2021). Classifying Adenomyosis: Progress and Challenges. Int. J. Environ. Res. Public Health, 18.","DOI":"10.3390\/ijerph182312386"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Habiba, M., Guo, S.W., and Benagiano, G. (2023). Are Adenomyosis and Endometriosis Phenotypes of the Same Disease Process?. Biomolecules, 14.","DOI":"10.3390\/biom14010032"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1016\/j.jmig.2019.11.013","article-title":"Classification and Reporting Systems for Adenomyosis","volume":"27","author":"Munro","year":"2020","journal-title":"J. Minim. Invasive Gynecol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1016\/j.fertnstert.2018.01.035","article-title":"Introduction: Uterine adenomyosis, another enigmatic disease of our time","volume":"109","author":"Donnez","year":"2018","journal-title":"Fertil. Steril."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Moawad, G., Fruscalzo, A., Youssef, Y., Kheil, M., Tawil, T., Nehme, J., Pirtea, P., Guani, B., Afaneh, H., and Ayoubi, J.M. (2023). Adenomyosis: An Updated Review on Diagnosis and Classification. J. Clin. Med., 12.","DOI":"10.3390\/jcm12144828"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"S147","DOI":"10.1148\/radiographics.19.suppl_1.g99oc13s147","article-title":"Uterine adenomyosis: Endovaginal US and MR imaging features with histopathologic correlation","volume":"19","author":"Reinhold","year":"1999","journal-title":"Radiographics"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"114.e1","DOI":"10.1016\/j.ajog.2012.06.027","article-title":"Four subtypes of adenomyosis assessed by magnetic resonance imaging and their specification","volume":"207","author":"Kishi","year":"2012","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1016\/0002-9378(72)90781-8","article-title":"The elusive adenomyosis of the uterus\u2014Revisited","volume":"112","author":"Bird","year":"1972","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_25","first-page":"2221","article-title":"Adenomyosis: Current perspectives","volume":"16","author":"Azziz","year":"1989","journal-title":"Obstet. Gynecol. Clin. N. Am."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1016\/S0002-9378(84)90366-1","article-title":"Confirmation of the preoperative diagnoses for hysterectomy","volume":"150","author":"Lee","year":"1984","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"816","DOI":"10.1093\/ajcp\/75.6.816","article-title":"The use of antiavidin antibody and avidin-biotin-peroxidase complex in immunoperoxidase technics","volume":"75","author":"Hsu","year":"1981","journal-title":"Am. J. Clin. Pathol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1055\/s-0040-1718922","article-title":"Pathology and Pathogenesis of Adenomyosis","volume":"38","author":"Antero","year":"2020","journal-title":"Semin. Reprod. Med."},{"key":"ref_29","first-page":"1099","article-title":"Histopathological characteristics of adenomyosis: Structure and microstructure","volume":"38","author":"Liu","year":"2023","journal-title":"Histol. Histopathol."},{"key":"ref_30","first-page":"688","article-title":"Adenomyosis: Symptoms, Histology, and Pregnancy Terminations","volume":"95","author":"Levgur","year":"2000","journal-title":"Obstet. Gynecol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"e12442","DOI":"10.1002\/rmb2.12442","article-title":"How does the extent of fibrosis in adenomyosis lesions contribute to heavy menstrual bleeding?","volume":"21","author":"Huang","year":"2022","journal-title":"Reprod. Med. Biol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"113","DOI":"10.12891\/ceog16152014","article-title":"Distinct types of uterine adenomyosis based on laparoscopic and histopathologic criteria","volume":"41","author":"Pistofidis","year":"2014","journal-title":"Clin. Exp. Obstet. Gynecol."},{"key":"ref_33","first-page":"2258","article-title":"Expression and cellular localization of estrogen receptors \u03b1 and \u03b2 in the human fetus","volume":"86","author":"Takeyama","year":"2001","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/j.diff.2020.10.001","article-title":"Ontogeny of estrogen receptors in human male and female fetal reproductive tracts","volume":"118","author":"Cunha","year":"2021","journal-title":"Differentiation"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1016\/j.bpobgyn.2006.01.010","article-title":"Adenomyosis: The pathophysiology of an oestrogen-dependent disease","volume":"20","author":"Kitawaki","year":"2006","journal-title":"Best Pract. Res. Clin. Obstet. Gynaecol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"e42768","DOI":"10.1097\/MD.0000000000042768","article-title":"Identification of novel causally related genes in adenomyosis: An integrated summary data-based Mendelian randomization study and bioinformatics analysis","volume":"104","author":"Yang","year":"2025","journal-title":"Medicine"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"59","DOI":"10.3109\/00016349409013396","article-title":"Endometriosis in monozygotic twins","volume":"73","author":"Moen","year":"1994","journal-title":"Acta Obstet. Gynecol. Scand."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"941","DOI":"10.1016\/S0015-0282(97)00359-2","article-title":"Endometriosis in monozygotic twins","volume":"68","author":"Hadfield","year":"1997","journal-title":"Fertil. Steril."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Palumbo, M., Della Corte, L., Ascione, M., D\u2019Angelo, G., Colacurci, D., Baldini, G.M., Pellicano, M., Giampaolino, P., and Bifulco, G. (2025). Genetic and Epigenetic Components in the Pathogenesis of Adenomyosis and Endometriosis in Adolescents. Biomedicines, 13.","DOI":"10.3390\/biomedicines13122988"},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Giacomini, E., Minetto, S., Li Piani, L., Pagliardini, L., Somigliana, E., and Vigano, P. (2021). Genetics and Inflammation in Endometriosis: Improving Knowledge for Development of New Pharmacological Strategies. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22169033"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"3802","DOI":"10.1038\/s41596-021-00556-8","article-title":"CODEX multiplexed tissue imaging with DNA-conjugated antibodies","volume":"16","author":"Black","year":"2021","journal-title":"Nat. Protoc."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"100197","DOI":"10.1016\/j.modpat.2023.100197","article-title":"Multiplex Immunohistochemistry and Immunofluorescence: A Practical Update for Pathologists","volume":"36","author":"Harms","year":"2023","journal-title":"Mod. Pathol."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/8\/3595\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T09:38:47Z","timestamp":1776677927000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/8\/3595"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,17]]},"references-count":42,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2026,4]]}},"alternative-id":["ijms27083595"],"URL":"https:\/\/doi.org\/10.3390\/ijms27083595","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,17]]}}}