{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T09:02:33Z","timestamp":1785402153159,"version":"3.56.0"},"reference-count":96,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,4,18]],"date-time":"2026-04-18T00:00:00Z","timestamp":1776470400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Facultad de Ciencia de la Salud, Universidad Aut\u00f3noma de Tlaxcala"},{"id":[{"id":"https:\/\/ror.org\/021vseb03","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003141","name":"Secretar\u00eda de Ciencia, Humanidades, Tecnolog\u00eda e Innovaci\u00f3n","doi-asserted-by":"crossref","award":["CBF-2025-I-3072"],"award-info":[{"award-number":["CBF-2025-I-3072"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"crossref"}]},{"award":["CBF-2025-I-3072"],"award-info":[{"award-number":["CBF-2025-I-3072"]}],"id":[{"id":"https:\/\/ror.org\/059ex5q34","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Breast carcinoma is a major cause of cancer-related mortality among women worldwide. Identifying novel molecular targets remains essential, particularly for aggressive triple-negative breast cancer (TNBC). Leucine-rich alpha-2-glycoprotein 1 (LRG1) has been linked to tumor progression and angiogenesis, but its molecular mechanisms in breast cancer are poorly defined. We evaluated the effects of recombinant human LRG1 (rhLRG1) on cell viability and migration in MDA-MB-231 TNBC cells and performed transcriptomic profiling followed by functional enrichment analyses using GenArise, Cytoscape, and R-based tools. RhLRG1 treatment significantly increased cell viability and migration. Transcriptomic analysis revealed activation of key oncogenic cascades, including the PI3K\/AKT, MAPK, and RAS signaling pathways. Hub-gene analysis identified upregulated genes involved in proliferation (NRAS, STAT5B, IGF2), angiogenesis (PGF, ANGPT2), and apoptosis (CASP8, BAD), whereas downregulated genes were associated with apoptotic resistance (BCL2, MCL1) and adhesion (LAMB1, ITGB4). Functional enrichment highlighted LRG1\u2019s role in the bioinformatic analysis of differentially expressed genes that were obtained from microarray assays. LRG1 remodels the tumor microenvironment by promoting proliferation, angiogenesis, and apoptotic sensitivity while repressing resistance-related genes. These findings position LRG1 as a potential diagnostic biomarker and therapeutic target for advanced breast carcinoma.<\/jats:p>","DOI":"10.3390\/ijms27083613","type":"journal-article","created":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T08:24:23Z","timestamp":1776673463000},"page":"3613","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Identification and Integration of LRG1-Induced Differentially Expressed Gene (DEG) Hub Profiles in Breast Cancer Cells"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3841-2663","authenticated-orcid":false,"given":"Federico","family":"Osorio-Antonio","sequence":"first","affiliation":[{"name":"Departamento de Gen\u00e9tica M\u00e9dica, Hospital Infantil de Tlaxcala, Servicios de Salud del Instituto Mexicano del Seguro Social para el Bienestar, Tlaxcala 90606, Mexico"},{"name":"Facultad de Biotecnolog\u00eda, Universidad Popular Aut\u00f3noma del Estado de Puebla, Puebla 72410, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela Michel","family":"Diaz-Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Departamento de Fisiolog\u00eda, Biof\u00edsica y Neurociencias, Centro de Investigaci\u00f3n y de Estudios Avanzados del Instituto Polit\u00e9cnico Nacional, Ciudad de M\u00e9xico 07360, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8674-3953","authenticated-orcid":false,"given":"Gabriela Elizabeth","family":"Campos-Viguri","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Ciencia Aplicada y Tecnolog\u00eda Avanzada Unidad Morelos, Instituto Polit\u00e9cnico Nacional, Atlacholoaya 62790, Mexico"},{"name":"Direcci\u00f3n de Infecciones Cr\u00f3nicas y C\u00e1ncer, Centro de Investigaci\u00f3n Sobre Enfermedades Infecciosas, Instituto Nacional de Salud P\u00fablica, Cuernavaca 62100, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1349-4746","authenticated-orcid":false,"given":"Jos\u00e9 Manuel","family":"S\u00e1nchez-L\u00f3pez","sequence":"additional","affiliation":[{"name":"Departamento de Gen\u00e9tica M\u00e9dica, Hospital Infantil de Tlaxcala, Servicios de Salud del Instituto Mexicano del Seguro Social para el Bienestar, Tlaxcala 90606, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Luis","family":"Cortez-S\u00e1nchez","sequence":"additional","affiliation":[{"name":"Environmental Epigenetics and Mental Health Laboratory, Faculty of Chemical Biological Sciences, Autonomous University of Campeche, Campeche 24085, Mexico"},{"name":"Health Sciences, University of the Americas Puebla (UDLAP), Puebla 72810, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9835-3335","authenticated-orcid":false,"given":"Francisco","family":"Castel\u00e1n","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Celular y Fisiolog\u00eda, Unidad For\u00e1nea Tlaxcala, Instituto de Investigaciones Biom\u00e9dicas, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Tlaxcala 90070, Mexico"},{"name":"Centro Tlaxcala de Biolog\u00eda de la Conducta, Universidad Aut\u00f3noma de Tlaxcala, Tlaxcala 90070, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9553-2568","authenticated-orcid":false,"given":"Jes\u00fas Ramses","family":"Ch\u00e1vez-Rios","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Celular y Fisiolog\u00eda, Unidad For\u00e1nea Tlaxcala, Instituto de Investigaciones Biom\u00e9dicas, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Tlaxcala 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