{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T15:09:48Z","timestamp":1785424188392,"version":"3.56.0"},"reference-count":27,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T00:00:00Z","timestamp":1776643200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Ministry of Education, Culture, Sports, Science, and Technology","doi-asserted-by":"publisher","award":["25K14971"],"award-info":[{"award-number":["25K14971"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"award":["25K14971"],"award-info":[{"award-number":["25K14971"]}],"id":[{"id":"https:\/\/ror.org\/048rj2z13","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Excessive neutrophil extracellular trap (NET) formation (NETosis), frequently associated with reactive oxygen species (ROS), exacerbates cutaneous inflammation induced by acute ultraviolet B (UVB) exposure. Although hispidin has potent antioxidant activity, its protective effects against acute UVB-induced skin inflammation and its relationship with NET-associated responses remain unclear. We investigated the effects of topical hispidin on acute UVB-induced skin injury in mice and examined its effects on ROS-associated NET-related responses in differentiated HL-60 cells. In a mouse model, topical hispidin (0.1% and 0.5%) ameliorated UVB-induced skin damage in a dose-dependent manner, as evidenced by improved clinical and histological findings. Hispidin treatment was associated with reduced systemic oxidative stress and decreased cutaneous expression of CXCL2, C5a, IL-1\u03b2, NLRP3, Ly6G, PAD4, and citrullinated histone H3. In differentiated HL-60 cells, hispidin reduced ROS-associated signals and suppressed PMA-triggered extracellular DNA release, but did not suppress A23187-triggered extracellular DNA release under experimental conditions. Cell viability analysis showed that hispidin did not significantly affect differentiated HL-60 cell viability at tested concentrations under the present experimental conditions. Topical hispidin alleviates acute UVB-induced skin inflammation by suppressing neutrophil infiltration and NET-related inflammatory responses. Hispidin may therefore represent a promising candidate as a topical modulator of oxidative stress- and NET-associated skin inflammation.<\/jats:p>","DOI":"10.3390\/ijms27083667","type":"journal-article","created":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T13:22:47Z","timestamp":1776691367000},"page":"3667","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Hispidin Ameliorates Acute Ultraviolet B-Induced Skin Inflammation by Targeting Reactive Oxygen Species-Dependent Neutrophil Extracellular Trap Formation"],"prefix":"10.3390","volume":"27","author":[{"given":"Yuina","family":"Arakaki","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Faculty of Pharmaceutical Sciences, Suzuka University of Medical Science, Suzuka-City 513-8670, Mie, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koshi","family":"Tominaga","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Pharmaceutical Sciences, Suzuka University of Medical Science, Suzuka-City 513-8670, Mie, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keiichi","family":"Hiramoto","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Pharmaceutical Sciences, Suzuka University of Medical Science, Suzuka-City 513-8670, Mie, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-9308-1701","authenticated-orcid":false,"given":"Masashi","family":"Imai","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Pharmaceutical Sciences, Suzuka University of Medical Science, Suzuka-City 513-8670, Mie, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akihiro","family":"Morita","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Pharmaceutical Sciences, Suzuka University of Medical Science, Suzuka-City 513-8670, Mie, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6824-6408","authenticated-orcid":false,"given":"Tomonari","family":"Tsutsumi","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Pharmaceutical Sciences, Suzuka University of Medical Science, Suzuka-City 513-8670, Mie, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroyuki","family":"Yasuda","sequence":"additional","affiliation":[{"name":"Division of Pathological Sciences, Department of Pharmacology and Experimental Therapeutics, Kyoto Pharmaceutical University, Misasagi 5, Yamashina, Kyoto 607-8414, Kyoto Prefecture, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eisuke F.","family":"Sato","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Pharmaceutical Sciences, Suzuka University of Medical Science, Suzuka-City 513-8670, Mie, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,4,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/j.ajpath.2011.03.010","article-title":"The immune-modulating cytokine and endogenous Alarmin interleukin-33 is upregulated in skin exposed to inflammatory UVB radiation","volume":"179","author":"Byrne","year":"2011","journal-title":"Am. 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