{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T11:10:26Z","timestamp":1785409826804,"version":"3.56.0"},"reference-count":84,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T00:00:00Z","timestamp":1776729600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["PI22\/00556"],"award-info":[{"award-number":["PI22\/00556"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"award":["PI22\/00556"],"award-info":[{"award-number":["PI22\/00556"]}],"id":[{"id":"https:\/\/ror.org\/00ca2c886","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Classical Hodgkin lymphoma (cHL) is a paradigmatic example of a malignancy in which the tumor microenvironment (TME) plays a dominant role in disease biology. Malignant Hodgkin and Reed\u2013Sternberg (HRS) cells typically constitute only a small minority of the tumor mass (approximately 1\u20135%). HRS cells are embedded within a complex, highly structured immune and stromal milieu that drives survival, immune evasion, and therapy response. Over the past decade, transcriptomic approaches, particularly single-cell RNA sequencing, have reshaped our understanding of cellular heterogeneity within cHL. However, these approaches lack spatial context, a limitation that is especially relevant in cHL, where cell\u2013cell interactions and physical proximity are central to immune evasion and tumor support. Recent advances in spatial transcriptomics now enable genome-scale, spatially resolved interrogation of gene expression in situ. In this review, we summarize spatially resolved studies of the cHL microenvironment, discuss what they reveal about HRS-centered cellular niches and immune evasion, and highlight how these findings may inform risk stratification, biomarker discovery and microenvironment-directed therapies.<\/jats:p>","DOI":"10.3390\/ijms27083689","type":"journal-article","created":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T11:42:45Z","timestamp":1776771765000},"page":"3689","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The Hodgkin Lymphoma Microenvironment: Insights from Spatial Transcriptomics"],"prefix":"10.3390","volume":"27","author":[{"given":"Ruth","family":"Alonso-Alonso","sequence":"first","affiliation":[{"name":"Translational Research, MD Anderson Cancer Center Foundation, 28033 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6338-1811","authenticated-orcid":false,"given":"Victoria","family":"Menendez","sequence":"additional","affiliation":[{"name":"uc3m\u2014Santander Big Data Institute (IBiDat), 28903 Getafe, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva M.","family":"V\u00e1zquez","sequence":"additional","affiliation":[{"name":"Pathology and Molecular Diagnostic Department, MD Anderson Cancer Center, 28033 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 L.","family":"Sol\u00f3rzano","sequence":"additional","affiliation":[{"name":"Pathology and Molecular Diagnostic Department, MD Anderson Cancer Center, 28033 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6974-0806","authenticated-orcid":false,"given":"Juan F.","family":"Garc\u00eda","sequence":"additional","affiliation":[{"name":"Translational Research, MD Anderson Cancer Center Foundation, 28033 Madrid, Spain"},{"name":"Pathology and Molecular Diagnostic Department, MD Anderson Cancer Center, 28033 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,4,21]]},"reference":[{"key":"ref_1","first-page":"229","article-title":"Global cancer statistics 2022: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries","volume":"74","author":"Bray","year":"2024","journal-title":"CA Cancer J. 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